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The map label for this gene is argF [H]

Identifier: 220904941

GI number: 220904941

Start: 2014197

End: 2015216

Strand: Direct

Name: argF [H]

Synonym: Ddes_1676

Alternate gene names: 220904941

Gene position: 2014197-2015216 (Clockwise)

Preceding gene: 220904940

Following gene: 220904942

Centisome position: 70.1

GC content: 54.8

Gene sequence:

>1020_bases
ATGAACAGACTACAACACAGAGACTTTCTGAAAGAAATCGATTTCACGCCGGAAGACCTCACCTACCTGCTGGACCTGGC
TGCCAATCTCAAACAGGCCAAAAAATCCCGCCGGGAACCCGAGTTTCTGAAGGGCAGGAACATTGTTATTCTTTTTGAAA
AAGACTCCACCCGTACCCGCTGTTCTTTTGAGGTGGCCGCCTATGATCAGGGCGCGCGCGTGACCTACCTTGGACCTTCC
GGTTCACAAATGGGCAAAAAAGAATCCCTGGCCGATACGGCCCGTGTGCTTTCCCGCTTTTATGACGGCATTGAATATCG
CGGCTTCGGACAGGAAAGGGTCGAGGCGCTGGCCGAGCATGCCTCGGTACCGGTATGGAACGGCCTGACCAATGAATGGC
ATCCCACCCAGTTCCTGGCAGACATGCTCACCATGCGCGAATGCTGCTCCAAGCCCCTGAACAGGCAAACCCTGGCCTAT
ATGGGTGACGCCCGCTATAACATGGGTAATTCTCTTATGATCGGCTCCGCCCTTCTGGGGCTGGATTTTCGTTCTGTGGC
ACCAAAGGCGCTGTGGACAGCGGACGAAGTTTACGAGATGGCCTGCCACATCGCCTCCGGCACTGGCGCGCGCATCAGCC
GCACCGAAAACGTGGTGGAAGGCGTACAGGGCTGCGACTTCATTTCTACAGACGTCTGGGTATCCATGGGCGAACCCGAT
GACGTGTGGAAAGAACGCATTGAACTGCTCACGCCCTATCGTGTAACGGGCGACACCATGCGGCAGACCGGCAATGACGA
CTGCAAGTTCTTGCATTGCCTGCCAAGTTTTCATAACCGTGACACAAGCATGGGCGAAGAGATTTTCCAGCGCTTCGGCA
TAGAATGCATGGAAGTCAACGACGAGGTTTTCGAGTCACCACGCAATGTGGCCTTTGAAGAAGCCGAAAATCGCCTTCAC
ACCATCAAGGCGGTCATGGTCGCCACATTGGCCGAAAGCCCGCTTGTTTTCAACGTATAG

Upstream 100 bases:

>100_bases
ACAAGCGGCGCAATGGCGGCCAGCCCAACGTGCTCAAGCACAACCCGCCCGCGCCCCGGCGCGGATGGCAGGACCCACTA
CCTCAAAGGCGAAACAGCGC

Downstream 100 bases:

>100_bases
TCTGAAAAAGCTGGAAGAAACAGGGCGGCACAAATAGACCGAACCACACATCCCACAGGGGGCATACCTCGTCCGCCTTG
CTACGCAAGGTGCCTGCAGG

Product: ornithine carbamoyltransferase

Products: NA

Alternate protein names: OTCase [H]

Number of amino acids: Translated: 339; Mature: 339

Protein sequence:

>339_residues
MNRLQHRDFLKEIDFTPEDLTYLLDLAANLKQAKKSRREPEFLKGRNIVILFEKDSTRTRCSFEVAAYDQGARVTYLGPS
GSQMGKKESLADTARVLSRFYDGIEYRGFGQERVEALAEHASVPVWNGLTNEWHPTQFLADMLTMRECCSKPLNRQTLAY
MGDARYNMGNSLMIGSALLGLDFRSVAPKALWTADEVYEMACHIASGTGARISRTENVVEGVQGCDFISTDVWVSMGEPD
DVWKERIELLTPYRVTGDTMRQTGNDDCKFLHCLPSFHNRDTSMGEEIFQRFGIECMEVNDEVFESPRNVAFEEAENRLH
TIKAVMVATLAESPLVFNV

Sequences:

>Translated_339_residues
MNRLQHRDFLKEIDFTPEDLTYLLDLAANLKQAKKSRREPEFLKGRNIVILFEKDSTRTRCSFEVAAYDQGARVTYLGPS
GSQMGKKESLADTARVLSRFYDGIEYRGFGQERVEALAEHASVPVWNGLTNEWHPTQFLADMLTMRECCSKPLNRQTLAY
MGDARYNMGNSLMIGSALLGLDFRSVAPKALWTADEVYEMACHIASGTGARISRTENVVEGVQGCDFISTDVWVSMGEPD
DVWKERIELLTPYRVTGDTMRQTGNDDCKFLHCLPSFHNRDTSMGEEIFQRFGIECMEVNDEVFESPRNVAFEEAENRLH
TIKAVMVATLAESPLVFNV
>Mature_339_residues
MNRLQHRDFLKEIDFTPEDLTYLLDLAANLKQAKKSRREPEFLKGRNIVILFEKDSTRTRCSFEVAAYDQGARVTYLGPS
GSQMGKKESLADTARVLSRFYDGIEYRGFGQERVEALAEHASVPVWNGLTNEWHPTQFLADMLTMRECCSKPLNRQTLAY
MGDARYNMGNSLMIGSALLGLDFRSVAPKALWTADEVYEMACHIASGTGARISRTENVVEGVQGCDFISTDVWVSMGEPD
DVWKERIELLTPYRVTGDTMRQTGNDDCKFLHCLPSFHNRDTSMGEEIFQRFGIECMEVNDEVFESPRNVAFEEAENRLH
TIKAVMVATLAESPLVFNV

Specific function: Arginine biosynthesis; sixth step. [C]

COG id: COG0078

COG function: function code E; Ornithine carbamoyltransferase

Gene ontology:

Cell location: Cytoplasm (Probable) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the ATCase/OTCase family [H]

Homologues:

Organism=Homo sapiens, GI38788445, Length=334, Percent_Identity=36.8263473053892, Blast_Score=194, Evalue=1e-49,
Organism=Homo sapiens, GI18105007, Length=339, Percent_Identity=25.6637168141593, Blast_Score=80, Evalue=2e-15,
Organism=Escherichia coli, GI1790703, Length=334, Percent_Identity=60.4790419161677, Blast_Score=414, Evalue=1e-117,
Organism=Escherichia coli, GI1786469, Length=334, Percent_Identity=58.3832335329341, Blast_Score=400, Evalue=1e-113,
Organism=Escherichia coli, GI2367364, Length=208, Percent_Identity=35.5769230769231, Blast_Score=83, Evalue=2e-17,
Organism=Escherichia coli, GI48994908, Length=381, Percent_Identity=23.6220472440945, Blast_Score=77, Evalue=2e-15,
Organism=Saccharomyces cerevisiae, GI6322373, Length=328, Percent_Identity=28.0487804878049, Blast_Score=131, Evalue=2e-31,
Organism=Saccharomyces cerevisiae, GI6322331, Length=166, Percent_Identity=31.9277108433735, Blast_Score=78, Evalue=2e-15,
Organism=Drosophila melanogaster, GI24642586, Length=188, Percent_Identity=32.4468085106383, Blast_Score=74, Evalue=2e-13,

Paralogues:

None

Copy number: 140 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 2066 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR006132
- InterPro:   IPR006130
- InterPro:   IPR006131
- InterPro:   IPR002292 [H]

Pfam domain/function: PF00185 OTCace; PF02729 OTCace_N [H]

EC number: =2.1.3.3 [H]

Molecular weight: Translated: 38431; Mature: 38431

Theoretical pI: Translated: 4.89; Mature: 4.89

Prosite motif: PS00097 CARBAMOYLTRANSFERASE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.4 %Cys     (Translated Protein)
3.8 %Met     (Translated Protein)
6.2 %Cys+Met (Translated Protein)
2.4 %Cys     (Mature Protein)
3.8 %Met     (Mature Protein)
6.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNRLQHRDFLKEIDFTPEDLTYLLDLAANLKQAKKSRREPEFLKGRNIVILFEKDSTRTR
CCCCHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHCCCCHHHCCCCEEEEEECCCCCCE
CSFEVAAYDQGARVTYLGPSGSQMGKKESLADTARVLSRFYDGIEYRGFGQERVEALAEH
EEEEEEEECCCCEEEEECCCCCCCCCHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHH
ASVPVWNGLTNEWHPTQFLADMLTMRECCSKPLNRQTLAYMGDARYNMGNSLMIGSALLG
CCCCCCCCCCCCCCHHHHHHHHHHHHHHHCCCCCCCHHHHHCCCCCCCCCCEEHHHHHHC
LDFRSVAPKALWTADEVYEMACHIASGTGARISRTENVVEGVQGCDFISTDVWVSMGEPD
CCHHHHCCHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHCCCCEECCCEEEECCCCH
DVWKERIELLTPYRVTGDTMRQTGNDDCKFLHCLPSFHNRDTSMGEEIFQRFGIECMEVN
HHHHHHHHHHCCCCCCCHHHHHCCCCCCCEEEECCCCCCCCHHHHHHHHHHHCCEEEECC
DEVFESPRNVAFEEAENRLHTIKAVMVATLAESPLVFNV
HHHHHCCCCCHHHHHHHHHHHHHHHHHHHHCCCCEEECC
>Mature Secondary Structure
MNRLQHRDFLKEIDFTPEDLTYLLDLAANLKQAKKSRREPEFLKGRNIVILFEKDSTRTR
CCCCHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHCCCCHHHCCCCEEEEEECCCCCCE
CSFEVAAYDQGARVTYLGPSGSQMGKKESLADTARVLSRFYDGIEYRGFGQERVEALAEH
EEEEEEEECCCCEEEEECCCCCCCCCHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHH
ASVPVWNGLTNEWHPTQFLADMLTMRECCSKPLNRQTLAYMGDARYNMGNSLMIGSALLG
CCCCCCCCCCCCCCHHHHHHHHHHHHHHHCCCCCCCHHHHHCCCCCCCCCCEEHHHHHHC
LDFRSVAPKALWTADEVYEMACHIASGTGARISRTENVVEGVQGCDFISTDVWVSMGEPD
CCHHHHCCHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHCCCCEECCCEEEECCCCH
DVWKERIELLTPYRVTGDTMRQTGNDDCKFLHCLPSFHNRDTSMGEEIFQRFGIECMEVN
HHHHHHHHHHCCCCCCCHHHHHCCCCCCCEEEECCCCCCCCHHHHHHHHHHHCCEEEECC
DEVFESPRNVAFEEAENRLHTIKAVMVATLAESPLVFNV
HHHHHCCCCCHHHHHHHHHHHHHHHHHHHHCCCCEEECC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 10952301 [H]