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The map label for this gene is phsA [H]

Identifier: 220904932

GI number: 220904932

Start: 2002500

End: 2004614

Strand: Reverse

Name: phsA [H]

Synonym: Ddes_1667

Alternate gene names: 220904932

Gene position: 2004614-2002500 (Counterclockwise)

Preceding gene: 220904933

Following gene: 220904931

Centisome position: 69.76

GC content: 57.97

Gene sequence:

>2115_bases
ATGAATGGTAATCAACTTACCCACAGTGTCTGCGGCATGTGTTCAGCGCGTTGCCCCATTACGGTTGAGACCTGTAATGA
CACTGTAAAAATGCTTTACGGCAACCTGCAAAGCCCTCTGAAGGGTGCATTATGCGCTCGTGGTGTGGCCGGAAAAGCTC
TTCTTGAAGACAATGAGCGTCCACAGTCGCCACTTATTCGCCAGGGGGCGCGCGGCGAAGGAAAATGGCGTGCCGTGTCC
TGGGACGAAGCTTTGGACCACGTTGCGCAAAAAATCACCGAAGCTCAAAACAGGTACGGCAGGCAGACCGTTCTCTGGTC
TGACCGAGAGGGGCCTTTTACCGACCTCAGCCGGGGATTCATGCGCGGTCTTGGTTCGCCCAACGTCTGTTCGCACAGTC
CTTCCTGTGATCTTAACGCGCATCATGCTTGCAAGGCCGTGCTGGGTCTGGGGCGCGGCATGACCGTGTATGATTTTGCC
AATGCCAAGCATATTGTTCTGCAGACGCGCAATATCTTTGAAGCCATCAATCTTGGCGAGGCGCGCACAGTCATGCAGGC
TCTGCGCAAGGGATGCAAGCTTACCGTCATTGACATCAGACAAAACGTGACTTCTTCCAAGGCAGACAAGTTCCATATCA
TCCGCCCCGGTACGGACTACGCCTTCAATCTGGCTGTCATCAATACCCTGATCAGCGAAAATCTGTACAACAAGGAATAT
GTCCGCGCTCATACCACGGGTTTTGATGCGCTGGCCGCCTTTGTGGCCCCATATACGGCGCAGTGGGCGGCGCAGGAGTG
CGGTATCGAGCCGCGGGCCATCACCGATCTCGCGCACGCGCTGGCTGCCGCGGCCCCCCAGGTTATCTGGCATCCGGGGT
GGATGACGTCGCGCTACGCCGATTCGTTTCAGGTGGGGCGCACGGCGCTGGTCATTACGGCGCTTTTGGGCGGCACGGGC
GCCAAGGGCGGCATTGTGCCCGGGCGGACTCCCAAGGACTGCGGCAAGTCCGGACTCAAGAAGTTTGTGGACCTGTATCC
TGCTGTCAAATTGCCCAGAGCCGACGGGCTTGGTTTTGAGAACAAGGCTTTTGATCCGGGCAAAGGGCTGCTGCACAAGG
CTTTTGATGCTATCAGCAGCCCCCCGGAAGGTGTGCCGCCGGTCAAGGTTTACATGGCCTGGCGCCATGACCCCTTGCAG
GGTTTTCCCGACCCCGATGCCCTCAAGAAAAAGCTGGACGGCCTGGATCTTCTGGTCAGCACCACGTTTTCCTGGTCCGA
CACGGCCTGGTATGCCGATGTGGTTTTACCCATGTCCACCTATCTTGAAAGAGAAAGCATCATCGCAGGCAAAAACGGTC
TCAAACCGCAGTTTTTTGTGCGCCGCCGGGCCGTGCAGCCCCGCTATGACACCCGTGCCGACTGGGAGATCATCAGTGGT
CTGTCCCGTCGGCTCGGCCTGGACAGTCTGGTTTTTGACAGCGCCGAGGCGGTCTGGAACTTCCAGCTTGAAGGCACGGG
GCTGACCATAGAAGATTTTGACGCCAAGGGCTTTATATCCCTCACGGACGATGCCCTGTACGTTGATCAGTCCACATATG
CCTTTCCCACCGGCTCCGGCAAGGTTGAACTGAGCAGCGAAAGTTACGGCAAAGGGTTTGCCGAAAATGCGGGCATCAGC
ATGCTGCCTCCGTACATTTCGCCCCAGTCGCCGCCGGAAGGAACCTTTCGTATCACCTTCGGGCGTGTGGCCGTGCACAC
GCAGGGGCATACAGTCAATAATCCCCTGCTGTACGAGCAGGTTCCGGAAAATACGGTGTGGATCAATACTGACAGCGCCA
AAAGGGCAGGATTAAAACCCGGCGACCGGGTGCGGGTGCTCGATGCCAGGGGCGGCAATATGGGCGAGGCAGGCATCAAG
ATCACGGCCTTTATCCACCCCGAGGCGGTGTTTGTCGTGCATGGCTTCGGCCATGACCTGCCGTGCGAGAGCCTGGCTGT
GGACAAGGGCATTGCCGACAACAAGTGCCTCAAGGGCGGGCTGGATCTGCAGGATCAGGGCGGTGGCGGCCTGTCGCTGC
AAGAACACTTCGTTTCGCTTGAGAAAGTGGGCTAG

Upstream 100 bases:

>100_bases
AATACTCACTGCTTCTATTCTGCCGGTTCGGTTGTCAGGGGCAAAAGATGACTTATAAAGCATCTTTATGGTACATTCTT
TGTCAGGTGAGAGATGCTCT

Downstream 100 bases:

>100_bases
CTGCGGCGGATACAGCCGGGCAGTCTAACCATATCCGGTTTGCAAGCTATGGGGGCGTGAACCCCGGAATAGCGGAGGAT
GTTTATGAGCAAGTATGTCG

Product: Formate dehydrogenase

Products: CO2; NADH; H2 [C]

Alternate protein names: NA

Number of amino acids: Translated: 704; Mature: 704

Protein sequence:

>704_residues
MNGNQLTHSVCGMCSARCPITVETCNDTVKMLYGNLQSPLKGALCARGVAGKALLEDNERPQSPLIRQGARGEGKWRAVS
WDEALDHVAQKITEAQNRYGRQTVLWSDREGPFTDLSRGFMRGLGSPNVCSHSPSCDLNAHHACKAVLGLGRGMTVYDFA
NAKHIVLQTRNIFEAINLGEARTVMQALRKGCKLTVIDIRQNVTSSKADKFHIIRPGTDYAFNLAVINTLISENLYNKEY
VRAHTTGFDALAAFVAPYTAQWAAQECGIEPRAITDLAHALAAAAPQVIWHPGWMTSRYADSFQVGRTALVITALLGGTG
AKGGIVPGRTPKDCGKSGLKKFVDLYPAVKLPRADGLGFENKAFDPGKGLLHKAFDAISSPPEGVPPVKVYMAWRHDPLQ
GFPDPDALKKKLDGLDLLVSTTFSWSDTAWYADVVLPMSTYLERESIIAGKNGLKPQFFVRRRAVQPRYDTRADWEIISG
LSRRLGLDSLVFDSAEAVWNFQLEGTGLTIEDFDAKGFISLTDDALYVDQSTYAFPTGSGKVELSSESYGKGFAENAGIS
MLPPYISPQSPPEGTFRITFGRVAVHTQGHTVNNPLLYEQVPENTVWINTDSAKRAGLKPGDRVRVLDARGGNMGEAGIK
ITAFIHPEAVFVVHGFGHDLPCESLAVDKGIADNKCLKGGLDLQDQGGGGLSLQEHFVSLEKVG

Sequences:

>Translated_704_residues
MNGNQLTHSVCGMCSARCPITVETCNDTVKMLYGNLQSPLKGALCARGVAGKALLEDNERPQSPLIRQGARGEGKWRAVS
WDEALDHVAQKITEAQNRYGRQTVLWSDREGPFTDLSRGFMRGLGSPNVCSHSPSCDLNAHHACKAVLGLGRGMTVYDFA
NAKHIVLQTRNIFEAINLGEARTVMQALRKGCKLTVIDIRQNVTSSKADKFHIIRPGTDYAFNLAVINTLISENLYNKEY
VRAHTTGFDALAAFVAPYTAQWAAQECGIEPRAITDLAHALAAAAPQVIWHPGWMTSRYADSFQVGRTALVITALLGGTG
AKGGIVPGRTPKDCGKSGLKKFVDLYPAVKLPRADGLGFENKAFDPGKGLLHKAFDAISSPPEGVPPVKVYMAWRHDPLQ
GFPDPDALKKKLDGLDLLVSTTFSWSDTAWYADVVLPMSTYLERESIIAGKNGLKPQFFVRRRAVQPRYDTRADWEIISG
LSRRLGLDSLVFDSAEAVWNFQLEGTGLTIEDFDAKGFISLTDDALYVDQSTYAFPTGSGKVELSSESYGKGFAENAGIS
MLPPYISPQSPPEGTFRITFGRVAVHTQGHTVNNPLLYEQVPENTVWINTDSAKRAGLKPGDRVRVLDARGGNMGEAGIK
ITAFIHPEAVFVVHGFGHDLPCESLAVDKGIADNKCLKGGLDLQDQGGGGLSLQEHFVSLEKVG
>Mature_704_residues
MNGNQLTHSVCGMCSARCPITVETCNDTVKMLYGNLQSPLKGALCARGVAGKALLEDNERPQSPLIRQGARGEGKWRAVS
WDEALDHVAQKITEAQNRYGRQTVLWSDREGPFTDLSRGFMRGLGSPNVCSHSPSCDLNAHHACKAVLGLGRGMTVYDFA
NAKHIVLQTRNIFEAINLGEARTVMQALRKGCKLTVIDIRQNVTSSKADKFHIIRPGTDYAFNLAVINTLISENLYNKEY
VRAHTTGFDALAAFVAPYTAQWAAQECGIEPRAITDLAHALAAAAPQVIWHPGWMTSRYADSFQVGRTALVITALLGGTG
AKGGIVPGRTPKDCGKSGLKKFVDLYPAVKLPRADGLGFENKAFDPGKGLLHKAFDAISSPPEGVPPVKVYMAWRHDPLQ
GFPDPDALKKKLDGLDLLVSTTFSWSDTAWYADVVLPMSTYLERESIIAGKNGLKPQFFVRRRAVQPRYDTRADWEIISG
LSRRLGLDSLVFDSAEAVWNFQLEGTGLTIEDFDAKGFISLTDDALYVDQSTYAFPTGSGKVELSSESYGKGFAENAGIS
MLPPYISPQSPPEGTFRITFGRVAVHTQGHTVNNPLLYEQVPENTVWINTDSAKRAGLKPGDRVRVLDARGGNMGEAGIK
ITAFIHPEAVFVVHGFGHDLPCESLAVDKGIADNKCLKGGLDLQDQGGGGLSLQEHFVSLEKVG

Specific function: Oxidoreductase which produces hydrogen sulfide from thiosulfate (Potential) [H]

COG id: COG0243

COG function: function code C; Anaerobic dehydrogenases, typically selenocysteine-containing

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the prokaryotic molybdopterin-containing oxidoreductase family [H]

Homologues:

Organism=Escherichia coli, GI3868721, Length=532, Percent_Identity=25.3759398496241, Blast_Score=144, Evalue=2e-35,
Organism=Escherichia coli, GI171474008, Length=730, Percent_Identity=23.5616438356164, Blast_Score=127, Evalue=3e-30,
Organism=Escherichia coli, GI1787870, Length=756, Percent_Identity=24.2063492063492, Blast_Score=126, Evalue=4e-30,
Organism=Escherichia coli, GI1787231, Length=646, Percent_Identity=23.6842105263158, Blast_Score=118, Evalue=1e-27,
Organism=Escherichia coli, GI3868720, Length=270, Percent_Identity=30, Blast_Score=87, Evalue=3e-18,
Organism=Escherichia coli, GI87081994, Length=534, Percent_Identity=22.0973782771536, Blast_Score=79, Evalue=1e-15,
Organism=Escherichia coli, GI3868719, Length=267, Percent_Identity=28.4644194756554, Blast_Score=69, Evalue=1e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR009010
- InterPro:   IPR006657
- InterPro:   IPR006656
- InterPro:   IPR006963
- InterPro:   IPR006655
- InterPro:   IPR006311 [H]

Pfam domain/function: PF04879 Molybdop_Fe4S4; PF00384 Molybdopterin; PF01568 Molydop_binding [H]

EC number: 1.2.1.2 [C]

Molecular weight: Translated: 76506; Mature: 76506

Theoretical pI: Translated: 7.55; Mature: 7.55

Prosite motif: PS00490 MOLYBDOPTERIN_PROK_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.8 %Cys     (Translated Protein)
1.6 %Met     (Translated Protein)
3.4 %Cys+Met (Translated Protein)
1.8 %Cys     (Mature Protein)
1.6 %Met     (Mature Protein)
3.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNGNQLTHSVCGMCSARCPITVETCNDTVKMLYGNLQSPLKGALCARGVAGKALLEDNER
CCCCHHHHHHHHHHCCCCCEEEECCCHHHHHHHHHHCCHHHHHHHHCCCCCCHHHCCCCC
PQSPLIRQGARGEGKWRAVSWDEALDHVAQKITEAQNRYGRQTVLWSDREGPFTDLSRGF
CCCHHHHCCCCCCCCEEEECHHHHHHHHHHHHHHHHHHCCCEEEEECCCCCCHHHHHHHH
MRGLGSPNVCSHSPSCDLNAHHACKAVLGLGRGMTVYDFANAKHIVLQTRNIFEAINLGE
HHCCCCCCCCCCCCCCCCCHHHHHHHHHHCCCCCEEEEECCCCEEEEEHHHHHHHHCCCH
ARTVMQALRKGCKLTVIDIRQNVTSSKADKFHIIRPGTDYAFNLAVINTLISENLYNKEY
HHHHHHHHHCCCEEEEEEECCCCCCCCCCEEEEECCCCCCEEHHHHHHHHHHHCCCCCHH
VRAHTTGFDALAAFVAPYTAQWAAQECGIEPRAITDLAHALAAAAPQVIWHPGWMTSRYA
HEEECCCHHHHHHHHCCHHHHHHHHHCCCCCHHHHHHHHHHHHCCCCEEECCCCCCHHHC
DSFQVGRTALVITALLGGTGAKGGIVPGRTPKDCGKSGLKKFVDLYPAVKLPRADGLGFE
CHHHHCHHHHHHHHHHCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHCCCCCCCCCCCCCC
NKAFDPGKGLLHKAFDAISSPPEGVPPVKVYMAWRHDPLQGFPDPDALKKKLDGLDLLVS
CCCCCCCHHHHHHHHHHHCCCCCCCCCEEEEEEECCCCCCCCCCHHHHHHHHCCCEEEEE
TTFSWSDTAWYADVVLPMSTYLERESIIAGKNGLKPQFFVRRRAVQPRYDTRADWEIISG
ECCCCCCCCEEHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHCCCCCCCCCCCHHHHHH
LSRRLGLDSLVFDSAEAVWNFQLEGTGLTIEDFDAKGFISLTDDALYVDQSTYAFPTGSG
HHHHCCCHHHHHCCCCCEEEEEECCCCCEEECCCCCCEEEECCCEEEEECCCEECCCCCC
KVELSSESYGKGFAENAGISMLPPYISPQSPPEGTFRITFGRVAVHTQGHTVNNPLLYEQ
EEEECCCCCCCCCCCCCCCEECCCCCCCCCCCCCEEEEEEEEEEEEECCCCCCCCEEEEE
VPENTVWINTDSAKRAGLKPGDRVRVLDARGGNMGEAGIKITAFIHPEAVFVVHGFGHDL
CCCCEEEEECCCHHHCCCCCCCEEEEEECCCCCCCCCCEEEEEEECCCEEEEEECCCCCC
PCESLAVDKGIADNKCLKGGLDLQDQGGGGLSLQEHFVSLEKVG
CCHHHHHCCCCCCCHHHCCCCCCCCCCCCCCHHHHHHHHHHCCC
>Mature Secondary Structure
MNGNQLTHSVCGMCSARCPITVETCNDTVKMLYGNLQSPLKGALCARGVAGKALLEDNER
CCCCHHHHHHHHHHCCCCCEEEECCCHHHHHHHHHHCCHHHHHHHHCCCCCCHHHCCCCC
PQSPLIRQGARGEGKWRAVSWDEALDHVAQKITEAQNRYGRQTVLWSDREGPFTDLSRGF
CCCHHHHCCCCCCCCEEEECHHHHHHHHHHHHHHHHHHCCCEEEEECCCCCCHHHHHHHH
MRGLGSPNVCSHSPSCDLNAHHACKAVLGLGRGMTVYDFANAKHIVLQTRNIFEAINLGE
HHCCCCCCCCCCCCCCCCCHHHHHHHHHHCCCCCEEEEECCCCEEEEEHHHHHHHHCCCH
ARTVMQALRKGCKLTVIDIRQNVTSSKADKFHIIRPGTDYAFNLAVINTLISENLYNKEY
HHHHHHHHHCCCEEEEEEECCCCCCCCCCEEEEECCCCCCEEHHHHHHHHHHHCCCCCHH
VRAHTTGFDALAAFVAPYTAQWAAQECGIEPRAITDLAHALAAAAPQVIWHPGWMTSRYA
HEEECCCHHHHHHHHCCHHHHHHHHHCCCCCHHHHHHHHHHHHCCCCEEECCCCCCHHHC
DSFQVGRTALVITALLGGTGAKGGIVPGRTPKDCGKSGLKKFVDLYPAVKLPRADGLGFE
CHHHHCHHHHHHHHHHCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHCCCCCCCCCCCCCC
NKAFDPGKGLLHKAFDAISSPPEGVPPVKVYMAWRHDPLQGFPDPDALKKKLDGLDLLVS
CCCCCCCHHHHHHHHHHHCCCCCCCCCEEEEEEECCCCCCCCCCHHHHHHHHCCCEEEEE
TTFSWSDTAWYADVVLPMSTYLERESIIAGKNGLKPQFFVRRRAVQPRYDTRADWEIISG
ECCCCCCCCEEHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHCCCCCCCCCCCHHHHHH
LSRRLGLDSLVFDSAEAVWNFQLEGTGLTIEDFDAKGFISLTDDALYVDQSTYAFPTGSG
HHHHCCCHHHHHCCCCCEEEEEECCCCCEEECCCCCCEEEECCCEEEEECCCEECCCCCC
KVELSSESYGKGFAENAGISMLPPYISPQSPPEGTFRITFGRVAVHTQGHTVNNPLLYEQ
EEEECCCCCCCCCCCCCCCEECCCCCCCCCCCCCEEEEEEEEEEEEECCCCCCCCEEEEE
VPENTVWINTDSAKRAGLKPGDRVRVLDARGGNMGEAGIKITAFIHPEAVFVVHGFGHDL
CCCCEEEEECCCHHHCCCCCCCEEEEEECCCCCCCCCCEEEEEEECCCEEEEEECCCCCC
PCESLAVDKGIADNKCLKGGLDLQDQGGGGLSLQEHFVSLEKVG
CCHHHHHCCCCCCCHHHCCCCCCCCCCCCCCHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NAD+ [C]

Metal ions: Fe; Mo; Se [C]

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): 0.19 {3-pyridinecarboxaldehyde-NAD+}} 0.032 {NAD+}} 1.16 {3-acetylpyridine-NAD+}} 0.54 {deamino-NAD+}} 0.17 {thio-NAD+}} [C]

Substrates: Formate; NAD(+) [C]

Specific reaction: Formate + NAD(+) = CO2 + NADH. formate = CO2 + H2 [C]

General reaction: Redox reaction [C]

Inhibitor: Azide; Br-; Cl-; CN-; F-; HCO3-; HCOS-; NO2-; NO3-; OCN-; SCN- [C]

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 7751291; 11677609; 7737516 [H]