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The map label for this gene is phsB [H]

Identifier: 220904931

GI number: 220904931

Start: 2001909

End: 2002415

Strand: Reverse

Name: phsB [H]

Synonym: Ddes_1666

Alternate gene names: 220904931

Gene position: 2002415-2001909 (Counterclockwise)

Preceding gene: 220904932

Following gene: 220904930

Centisome position: 69.69

GC content: 61.74

Gene sequence:

>507_bases
ATGAGCAAGTATGTCGTCACCCACAACTCTGCGGAATGCATCGGCTGCAAGGCCTGTGAAGTGCAGTGTCGCAGTCTGCA
TAACGGCGGCGCCGGCGCATTTTTTTGCCGTATTCTCACCGTGGAGCAGAGCGAGCCGCGCCCAGGGCTGGGTTTTGTCT
ATACATCCTGTTTTCATTGCGAAAACCCCTGGTGCGTAAAGGCTTGTCCCACGGGGGCCATGCGCCGCCGCGAGGACGGC
ATCGTCTATGTGGAAAAAAAATTCTGCGTGGGCTGCAAGGCGTGCATTACCGCCTGCCCGTGGGCCGCACCCCAGTGGAA
TCCCGATACCGGCAAGGTGGACAAGTGCGACCTGTGCCGCGACCGCATAGACCAGGGGCTGCGCCCGGCCTGTGTGACGA
CCTGCGCCATGAGCTGCCTGGGATTCACGGTTCCCTCCGAGGCCAGCCAGGAGCGGCGGCAGCATTTTGCGGAGCAGATA
CAGAAACTCCGGCCCACGACGCGCTGA

Upstream 100 bases:

>100_bases
TGAGAAAGTGGGCTAGCTGCGGCGGATACAGCCGGGCAGTCTAACCATATCCGGTTTGCAAGCTATGGGGGCGTGAACCC
CGGAATAGCGGAGGATGTTT

Downstream 100 bases:

>100_bases
AAGCAGGGTCTTTACCCTGCCATTTGCTGCGCAGTGCAGGGGCGGCAATCTGGCGCACAAAGGCGCGCCTCTGTGCCGGA
AGCGCGGCTCACGAAGCCCG

Product: 4Fe-4S ferredoxin iron-sulfur binding domain-containing protein

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 168; Mature: 167

Protein sequence:

>168_residues
MSKYVVTHNSAECIGCKACEVQCRSLHNGGAGAFFCRILTVEQSEPRPGLGFVYTSCFHCENPWCVKACPTGAMRRREDG
IVYVEKKFCVGCKACITACPWAAPQWNPDTGKVDKCDLCRDRIDQGLRPACVTTCAMSCLGFTVPSEASQERRQHFAEQI
QKLRPTTR

Sequences:

>Translated_168_residues
MSKYVVTHNSAECIGCKACEVQCRSLHNGGAGAFFCRILTVEQSEPRPGLGFVYTSCFHCENPWCVKACPTGAMRRREDG
IVYVEKKFCVGCKACITACPWAAPQWNPDTGKVDKCDLCRDRIDQGLRPACVTTCAMSCLGFTVPSEASQERRQHFAEQI
QKLRPTTR
>Mature_167_residues
SKYVVTHNSAECIGCKACEVQCRSLHNGGAGAFFCRILTVEQSEPRPGLGFVYTSCFHCENPWCVKACPTGAMRRREDGI
VYVEKKFCVGCKACITACPWAAPQWNPDTGKVDKCDLCRDRIDQGLRPACVTTCAMSCLGFTVPSEASQERRQHFAEQIQ
KLRPTTR

Specific function: Electron transport protein for the PHS system oxidoreductase which produces hydrogen sulfide from thiosulfate (Potential) [H]

COG id: COG0437

COG function: function code C; Fe-S-cluster-containing hydrogenase components 1

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 3 4Fe-4S ferredoxin-type domains [H]

Homologues:

Organism=Escherichia coli, GI1787872, Length=186, Percent_Identity=33.3333333333333, Blast_Score=117, Evalue=3e-28,
Organism=Escherichia coli, GI1787122, Length=186, Percent_Identity=33.3333333333333, Blast_Score=116, Evalue=6e-28,
Organism=Escherichia coli, GI226510944, Length=143, Percent_Identity=38.4615384615385, Blast_Score=97, Evalue=5e-22,
Organism=Escherichia coli, GI2367345, Length=173, Percent_Identity=36.9942196531792, Blast_Score=95, Evalue=3e-21,
Organism=Escherichia coli, GI1787749, Length=168, Percent_Identity=32.1428571428571, Blast_Score=91, Evalue=3e-20,
Organism=Escherichia coli, GI1790326, Length=165, Percent_Identity=34.5454545454545, Blast_Score=90, Evalue=8e-20,
Organism=Escherichia coli, GI1789370, Length=180, Percent_Identity=26.6666666666667, Blast_Score=86, Evalue=2e-18,
Organism=Escherichia coli, GI1788811, Length=174, Percent_Identity=33.3333333333333, Blast_Score=81, Evalue=5e-17,
Organism=Escherichia coli, GI1787963, Length=129, Percent_Identity=33.3333333333333, Blast_Score=80, Evalue=7e-17,
Organism=Escherichia coli, GI1789067, Length=180, Percent_Identity=31.6666666666667, Blast_Score=79, Evalue=2e-16,
Organism=Escherichia coli, GI87082179, Length=163, Percent_Identity=33.1288343558282, Blast_Score=75, Evalue=2e-15,
Organism=Escherichia coli, GI1787478, Length=99, Percent_Identity=40.4040404040404, Blast_Score=72, Evalue=3e-14,
Organism=Escherichia coli, GI1787740, Length=81, Percent_Identity=43.2098765432099, Blast_Score=70, Evalue=6e-14,
Organism=Escherichia coli, GI87082180, Length=147, Percent_Identity=34.0136054421769, Blast_Score=69, Evalue=1e-13,
Organism=Escherichia coli, GI2367245, Length=154, Percent_Identity=31.1688311688312, Blast_Score=67, Evalue=5e-13,
Organism=Escherichia coli, GI1789079, Length=108, Percent_Identity=33.3333333333333, Blast_Score=60, Evalue=8e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001450
- InterPro:   IPR017896
- InterPro:   IPR017900 [H]

Pfam domain/function: PF00037 Fer4 [H]

EC number: NA

Molecular weight: Translated: 18663; Mature: 18531

Theoretical pI: Translated: 8.17; Mature: 8.17

Prosite motif: PS00198 4FE4S_FERREDOXIN

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

10.7 %Cys     (Translated Protein)
1.8 %Met     (Translated Protein)
12.5 %Cys+Met (Translated Protein)
10.8 %Cys     (Mature Protein)
1.2 %Met     (Mature Protein)
12.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSKYVVTHNSAECIGCKACEVQCRSLHNGGAGAFFCRILTVEQSEPRPGLGFVYTSCFHC
CCCEEEECCCCCEECCHHHHHHHHHCCCCCCCEEEEEEEEEECCCCCCCCCHHHHHEEEC
ENPWCVKACPTGAMRRREDGIVYVEKKFCVGCKACITACPWAAPQWNPDTGKVDKCDLCR
CCCEEEEECCCCHHHHCCCCEEEEEHHHHHHHHHHHHHCCCCCCCCCCCCCCCCHHHHHH
DRIDQGLRPACVTTCAMSCLGFTVPSEASQERRQHFAEQIQKLRPTTR
HHHHCCCCHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHCCCCC
>Mature Secondary Structure 
SKYVVTHNSAECIGCKACEVQCRSLHNGGAGAFFCRILTVEQSEPRPGLGFVYTSCFHC
CCEEEECCCCCEECCHHHHHHHHHCCCCCCCEEEEEEEEEECCCCCCCCCHHHHHEEEC
ENPWCVKACPTGAMRRREDGIVYVEKKFCVGCKACITACPWAAPQWNPDTGKVDKCDLCR
CCCEEEEECCCCHHHHCCCCEEEEEHHHHHHHHHHHHHCCCCCCCCCCCCCCCCHHHHHH
DRIDQGLRPACVTTCAMSCLGFTVPSEASQERRQHFAEQIQKLRPTTR
HHHHCCCCHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: 4Fe-4S clusters [C]

Metal ions: Iron-Sulfur [C]

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11677608; 12644504 [H]