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The map label for this gene is nuoB

Identifier: 220904929

GI number: 220904929

Start: 2000840

End: 2001391

Strand: Reverse

Name: nuoB

Synonym: Ddes_1664

Alternate gene names: 220904929

Gene position: 2001391-2000840 (Counterclockwise)

Preceding gene: 220904930

Following gene: 220904928

Centisome position: 69.65

GC content: 61.59

Gene sequence:

>552_bases
ATGGGTGAAGTAGTCACAAAGTCCGGGGGGGCTGTGCACACCGGCGGGCAGCCCCTTGAGGTCAGTAAGGACGGCCTGCG
TTTTTTTCCCGGCGCAGGCGCTGTTATCGGGCCGCTCAACGCTCTTGTGAACTGGGGCAGGGCGGGGTCCATCTGGCCCG
TGACCTTTGGGCTGGCCTGCTGTGCCATTGAAATGATGGCTACCGGGGCAGCCCACCACGATCTTGACCGTTTCGGCATT
ATTTTTCGTGCCAGCCCCCGGCAGGCAGACTGCATGGTGGTGGCGGGCACGTTAAGCAAAAAAATGGCTCCGGTGCTGCG
CCGCGTATACGACCAGATGCCCGAACCGCGCTACGTGCTCGCCATGGGCAGTTGTGCGTGTAGCGGCGGCCTGTTTCAGT
CTTACGCTGTGACCCAGGGCGTGGACCAGGTTGTGCCTGTGGATGTTTACGTGCCCGGCTGTCCTCCGCGCCCGGAGGCG
CTGTTTGACGGGTTTATCCGGCTTCAGGAGATCATCAACAAGGAGCAGATGCGATGGAGTCCCTGGAGATAG

Upstream 100 bases:

>100_bases
AGTTTGTGCGCCTTGGCAGCCTTGGCATGGTGGAGATGTTCACCTTTATGGCTCTGCTGGTTCTCGGCTACCTGTATGCC
TGGAAGAAGGGGGCGTTCCA

Downstream 100 bases:

>100_bases
CGGACCGCCTGCGCGGGTTTTTTCCTGAAGAAGTGCTGGACGTGCGCGAGTTTCGGGGGCAGCTGGCGGTTCTGGTGCGG
TCCGGCCGCATTCTGGAACT

Product: NADH-quinone oxidoreductase subunit B

Products: NA

Alternate protein names: NADH dehydrogenase I subunit B; NDH-1 subunit B

Number of amino acids: Translated: 183; Mature: 182

Protein sequence:

>183_residues
MGEVVTKSGGAVHTGGQPLEVSKDGLRFFPGAGAVIGPLNALVNWGRAGSIWPVTFGLACCAIEMMATGAAHHDLDRFGI
IFRASPRQADCMVVAGTLSKKMAPVLRRVYDQMPEPRYVLAMGSCACSGGLFQSYAVTQGVDQVVPVDVYVPGCPPRPEA
LFDGFIRLQEIINKEQMRWSPWR

Sequences:

>Translated_183_residues
MGEVVTKSGGAVHTGGQPLEVSKDGLRFFPGAGAVIGPLNALVNWGRAGSIWPVTFGLACCAIEMMATGAAHHDLDRFGI
IFRASPRQADCMVVAGTLSKKMAPVLRRVYDQMPEPRYVLAMGSCACSGGLFQSYAVTQGVDQVVPVDVYVPGCPPRPEA
LFDGFIRLQEIINKEQMRWSPWR
>Mature_182_residues
GEVVTKSGGAVHTGGQPLEVSKDGLRFFPGAGAVIGPLNALVNWGRAGSIWPVTFGLACCAIEMMATGAAHHDLDRFGII
FRASPRQADCMVVAGTLSKKMAPVLRRVYDQMPEPRYVLAMGSCACSGGLFQSYAVTQGVDQVVPVDVYVPGCPPRPEAL
FDGFIRLQEIINKEQMRWSPWR

Specific function: NDH-1 shuttles electrons from NADH, via FMN and iron- sulfur (Fe-S) centers, to quinones in the respiratory chain. The immediate electron acceptor for the enzyme in this species is believed to be ubiquinone. Couples the redox reaction to proton translocat

COG id: COG0377

COG function: function code C; NADH:ubiquinone oxidoreductase 20 kD subunit and related Fe-S oxidoreductases

Gene ontology:

Cell location: Cell inner membrane; Peripheral membrane protein; Cytoplasmic side

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the complex I 20 kDa subunit family

Homologues:

Organism=Homo sapiens, GI187281616, Length=143, Percent_Identity=61.5384615384615, Blast_Score=200, Evalue=7e-52,
Organism=Escherichia coli, GI1788624, Length=161, Percent_Identity=55.2795031055901, Blast_Score=190, Evalue=4e-50,
Organism=Escherichia coli, GI1789074, Length=106, Percent_Identity=42.4528301886792, Blast_Score=93, Evalue=1e-20,
Organism=Escherichia coli, GI1788834, Length=110, Percent_Identity=38.1818181818182, Blast_Score=86, Evalue=1e-18,
Organism=Caenorhabditis elegans, GI17509685, Length=142, Percent_Identity=55.6338028169014, Blast_Score=180, Evalue=3e-46,
Organism=Drosophila melanogaster, GI18859983, Length=140, Percent_Identity=60.7142857142857, Blast_Score=197, Evalue=4e-51,
Organism=Drosophila melanogaster, GI24642371, Length=140, Percent_Identity=60.7142857142857, Blast_Score=197, Evalue=4e-51,
Organism=Drosophila melanogaster, GI24651058, Length=137, Percent_Identity=60.5839416058394, Blast_Score=192, Evalue=1e-49,

Paralogues:

None

Copy number: 520 Molecules/Cell In: Stationary Phase, Rich Media (Based on E. coli). [C]

Swissprot (AC and ID): NUOB_DESDA (B8J1D6)

Other databases:

- EMBL:   CP001358
- RefSeq:   YP_002480241.1
- GeneID:   7285367
- GenomeReviews:   CP001358_GR
- KEGG:   dds:Ddes_1664
- HOGENOM:   HBG553221
- GO:   GO:0006810
- HAMAP:   MF_01356
- InterPro:   IPR006137
- InterPro:   IPR006138
- InterPro:   IPR014406
- PANTHER:   PTHR11995:SF2
- PANTHER:   PTHR11995
- TIGRFAMs:   TIGR01957

Pfam domain/function: PF01058 Oxidored_q6

EC number: =1.6.99.5

Molecular weight: Translated: 19754; Mature: 19623

Theoretical pI: Translated: 8.12; Mature: 8.12

Prosite motif: PS01150 COMPLEX1_20K

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

3.3 %Cys     (Translated Protein)
4.4 %Met     (Translated Protein)
7.7 %Cys+Met (Translated Protein)
3.3 %Cys     (Mature Protein)
3.8 %Met     (Mature Protein)
7.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MGEVVTKSGGAVHTGGQPLEVSKDGLRFFPGAGAVIGPLNALVNWGRAGSIWPVTFGLAC
CCCEECCCCCEEECCCCCCEECCCCCEECCCCCCHHHHHHHHHCCCCCCCCCHHHHHHHH
CAIEMMATGAAHHDLDRFGIIFRASPRQADCMVVAGTLSKKMAPVLRRVYDQMPEPRYVL
HHHHHHHCCCCCCCHHHHEEEEECCCCCCCEEEEECHHHHHHHHHHHHHHHHCCCCEEEE
AMGSCACSGGLFQSYAVTQGVDQVVPVDVYVPGCPPRPEALFDGFIRLQEIINKEQMRWS
EECCCCCCCCHHHHHHHHCCCCCEEEEEEEECCCCCCHHHHHHHHHHHHHHHCHHHCCCC
PWR
CCC
>Mature Secondary Structure 
GEVVTKSGGAVHTGGQPLEVSKDGLRFFPGAGAVIGPLNALVNWGRAGSIWPVTFGLAC
CCEECCCCCEEECCCCCCEECCCCCEECCCCCCHHHHHHHHHCCCCCCCCCHHHHHHHH
CAIEMMATGAAHHDLDRFGIIFRASPRQADCMVVAGTLSKKMAPVLRRVYDQMPEPRYVL
HHHHHHHCCCCCCCHHHHEEEEECCCCCCCEEEEECHHHHHHHHHHHHHHHHCCCCEEEE
AMGSCACSGGLFQSYAVTQGVDQVVPVDVYVPGCPPRPEALFDGFIRLQEIINKEQMRWS
EECCCCCCCCHHHHHHHHCCCCCEEEEEEEECCCCCCHHHHHHHHHHHHHHHCHHHCCCC
PWR
CCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: NA