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The map label for this gene is grpE [H]

Identifier: 220904326

GI number: 220904326

Start: 1234220

End: 1234843

Strand: Direct

Name: grpE [H]

Synonym: Ddes_1053

Alternate gene names: 220904326

Gene position: 1234220-1234843 (Clockwise)

Preceding gene: 220904322

Following gene: 220904332

Centisome position: 42.95

GC content: 55.45

Gene sequence:

>624_bases
ATGCAGCGTCACAAGATGCACAAGTCTTATGAAAGGGCGGCGCAGCCGGAAGACGAATTTTCGGAAGACGACCTGAATTT
TTGCGCGCCGGACGGCATGGGAGACATGCCTGGCGAATTGGACGATACCTTGCTGGAGAACATGTCTGAAGCGATGACAG
AGCAGGGCGACGTCTTTTCGGCGGCAGAAGTTGAAGCACGGTGCAAAGCCGAAGTGGAAGAAATGCGTTTGCGCATGGCT
GCTGAGATGGACAACTTTCAGAAGCGCCTCAAGCGTGAGCATGAAGAACAAATGCGTTACGCCGCTGAAAATGTCTTGGG
TGATCTTTTGCCCAGTCTTGACAATCTTGATCTGGCCTTGCAGTACGGCAGTACCAGTGAGGTTTGCAAGGACATGTTGC
AGGGCGTGGCCATGACACGCAAACTGCTTCTTGAAGCTGTTGCCAAGCACGGCCTTACCCCGGTGGGCGAAGAAGGCGAA
GAATTTGACCCAGCCATACATGAAGCCGTGGGTTTTGACGCCCGCCCGGAGCTTGCCCCCAATTCTGTGGCCCGCCTGCT
GCAGCGCGGGTACAAGCTTGGTGAGCGCCTGTTGCGCCCCGCAAAGGTTATGGTCAATCCCTGA

Upstream 100 bases:

>100_bases
TATACGTCAAGCCTCATATGGCCTTGACAGTCTGTGAACGCGAACTTAGTTACAACCAATGAAAGCGACCGTCCTTGGGC
GGGGCGACTCAGGAGGAATC

Downstream 100 bases:

>100_bases
CCGACACGTTCAGTGAAAGAAAAAAGGCCGTAACCGACGGGAACTCCGTCGATTACGGCCTTTTTTGGGGATACTCCAGA
CACCCTGTGCACGCAGCAGT

Product: GrpE protein

Products: NA

Alternate protein names: HSP-70 cofactor [H]

Number of amino acids: Translated: 207; Mature: 207

Protein sequence:

>207_residues
MQRHKMHKSYERAAQPEDEFSEDDLNFCAPDGMGDMPGELDDTLLENMSEAMTEQGDVFSAAEVEARCKAEVEEMRLRMA
AEMDNFQKRLKREHEEQMRYAAENVLGDLLPSLDNLDLALQYGSTSEVCKDMLQGVAMTRKLLLEAVAKHGLTPVGEEGE
EFDPAIHEAVGFDARPELAPNSVARLLQRGYKLGERLLRPAKVMVNP

Sequences:

>Translated_207_residues
MQRHKMHKSYERAAQPEDEFSEDDLNFCAPDGMGDMPGELDDTLLENMSEAMTEQGDVFSAAEVEARCKAEVEEMRLRMA
AEMDNFQKRLKREHEEQMRYAAENVLGDLLPSLDNLDLALQYGSTSEVCKDMLQGVAMTRKLLLEAVAKHGLTPVGEEGE
EFDPAIHEAVGFDARPELAPNSVARLLQRGYKLGERLLRPAKVMVNP
>Mature_207_residues
MQRHKMHKSYERAAQPEDEFSEDDLNFCAPDGMGDMPGELDDTLLENMSEAMTEQGDVFSAAEVEARCKAEVEEMRLRMA
AEMDNFQKRLKREHEEQMRYAAENVLGDLLPSLDNLDLALQYGSTSEVCKDMLQGVAMTRKLLLEAVAKHGLTPVGEEGE
EFDPAIHEAVGFDARPELAPNSVARLLQRGYKLGERLLRPAKVMVNP

Specific function: Participates actively in the response to hyperosmotic and heat shock by preventing the aggregation of stress-denatured proteins, in association with dnaK and grpE. It is the nucleotide exchange factor for dnaK and may function as a thermosensor. Unfolded

COG id: COG0576

COG function: function code O; Molecular chaperone GrpE (heat shock protein)

Gene ontology:

Cell location: Cytoplasm (Probable) [H]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the grpE family [H]

Homologues:

Organism=Escherichia coli, GI1788967, Length=130, Percent_Identity=33.0769230769231, Blast_Score=83, Evalue=1e-17,
Organism=Caenorhabditis elegans, GI17552458, Length=137, Percent_Identity=35.036496350365, Blast_Score=74, Evalue=4e-14,
Organism=Saccharomyces cerevisiae, GI6324806, Length=165, Percent_Identity=30.3030303030303, Blast_Score=80, Evalue=3e-16,
Organism=Drosophila melanogaster, GI24653432, Length=175, Percent_Identity=31.4285714285714, Blast_Score=76, Evalue=2e-14,

Paralogues:

None

Copy number: 2359 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. 4,000 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000740
- InterPro:   IPR013805
- InterPro:   IPR009012 [H]

Pfam domain/function: PF01025 GrpE [H]

EC number: NA

Molecular weight: Translated: 23257; Mature: 23257

Theoretical pI: Translated: 4.39; Mature: 4.39

Prosite motif: PS01071 GRPE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.4 %Cys     (Translated Protein)
6.3 %Met     (Translated Protein)
7.7 %Cys+Met (Translated Protein)
1.4 %Cys     (Mature Protein)
6.3 %Met     (Mature Protein)
7.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MQRHKMHKSYERAAQPEDEFSEDDLNFCAPDGMGDMPGELDDTLLENMSEAMTEQGDVFS
CCHHHHHHHHHHHCCCCCCCCCCCCCEECCCCCCCCCCHHHHHHHHHHHHHHHHCCCCHH
AAEVEARCKAEVEEMRLRMAAEMDNFQKRLKREHEEQMRYAAENVLGDLLPSLDNLDLAL
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHEE
QYGSTSEVCKDMLQGVAMTRKLLLEAVAKHGLTPVGEEGEEFDPAIHEAVGFDARPELAP
ECCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCHHHHHHHCCCCCCCCCH
NSVARLLQRGYKLGERLLRPAKVMVNP
HHHHHHHHHHHHHHHHHHHHHHHHCCC
>Mature Secondary Structure
MQRHKMHKSYERAAQPEDEFSEDDLNFCAPDGMGDMPGELDDTLLENMSEAMTEQGDVFS
CCHHHHHHHHHHHCCCCCCCCCCCCCEECCCCCCCCCCHHHHHHHHHHHHHHHHCCCCHH
AAEVEARCKAEVEEMRLRMAAEMDNFQKRLKREHEEQMRYAAENVLGDLLPSLDNLDLAL
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHEE
QYGSTSEVCKDMLQGVAMTRKLLLEAVAKHGLTPVGEEGEEFDPAIHEAVGFDARPELAP
ECCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCHHHHHHHCCCCCCCCCH
NSVARLLQRGYKLGERLLRPAKVMVNP
HHHHHHHHHHHHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA