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The map label for this gene is mdeA [H]

Identifier: 220904313

GI number: 220904313

Start: 1216949

End: 1218295

Strand: Direct

Name: mdeA [H]

Synonym: Ddes_1040

Alternate gene names: 220904313

Gene position: 1216949-1218295 (Clockwise)

Preceding gene: 220904309

Following gene: 220904316

Centisome position: 42.35

GC content: 55.83

Gene sequence:

>1347_bases
ATGCGCTTGCGTTATTTTCGTGCCGCGCGGCGCGAATGTATGGCCGTCATGATCTTTAAATTTCACCGCATTGAGGATGG
CATGAAAACGGAATCTCTTTGTCTGCACGCCGGGTATGAGCCTAAAAATGGTGAGCCGCGTGTTGTGCCTATCGCCCAGA
GCACCACATTCAGATATGAATCCACCGCAGCTGTGGCCAGGCTTTTTGACCTCGAGGATGCGGGATTTTTTTATACCCGC
CTTGGTAATCCCACAGTAGATGCGGTAGAGCGCAAGATCGCGGCCCTTGAAGGCGGCGTGGGCGCCCTTTGCACATCCTC
CGGGCAGGCGGCCAACCTGCTTGCCCTGCTTAATGTGGCGCGCAGCGGCGATCATATCGTCAGCGCTTCCAGCATTTATG
GGGGTACATTCAACCTTTTTGCCGTCACCCTGAAAAAGCTCGGCATTGAAGTAACATTTGTGGATCAGACCGCTTCGGAC
GAAGAGCTTGAAAAAGCCTTCAGGCCCAATACGAAAGCGGTGTTCGGAGAGGTTCTGACAAATCCGTCCATGGACGTGCT
GGACATAGAGCGTTTTGCCGGGCTGGCGCACAGGCATGGTCTGCCGCTTGTGGTGGACAGTACTTTTGCCACGCCCGTGC
TGTGCCGCCCCTTTGAATACGGGGCGGATGTGGTCGTGCACTCCACCACAAAGTATATGGACGGGCATGCTCTACAAATG
GGCGGTGTTATTGTGGATAGTGGGAATTTTGACTGGACTGGCGGCAAGTTTTCCGAATTCACCGAGCCGGATCCTTCCTA
TCACGGGCTGGTTTACACAGAAGCCTTTGGCCGTGCAGCCTATATCGTCAAGGCTCGTGTACAGCTTATGCGTGACATGG
GCTGCTGCCAAAGCCCACAGGGAGCCTTTTACATTAATCAGGGCCTTGAAACTCTGCCACTGCGCATGGAGCGCCATTGC
CGCAATGCCGAAACTGTGGCTGCCTACCTTGCAAACCATCCCAAGGTCGAGTCTGTCAGTTATCCACGTCTGCCGGGCAA
CGCCAACAAGGTGCTGGCCGATAAATACCTGCCCGACGGGTGCAGCGGCGTGATTTCCTTTTCGCTCGGCGGAGGGCGTG
ACGCCGGGGCGCGCTTTATCGACAGCCTTGCAATGGTTTCACTTGAAGTGCACGTGGCTGACATTCGCACCTGCGTATTA
CACCCGGCAAGCTCCACCCACCGGCAGTTGACCGATGAGCAATTGCGCGAGGCGGGCATAAATCCCGGCACAGTGCGTTT
GTCTGTGGGGCTTGAGCATGTGGATGACATTATTGCGGATCTGGACCAGGCACTCAAGCAGGCGTAG

Upstream 100 bases:

>100_bases
TAAAAAACTTTGCCCGGGCGGCCATCAGGGTTGTCTTGCGGGGGTATGTCATGCGTGTCGAAGGGTGGATCCGGAGCTTG
CGCGTCAGGTTGCCACCCGG

Downstream 100 bases:

>100_bases
CCGCTGTTGCCATAATATGCGGGCCATAAAAAATATATGGGCCATAAAAAAGGCGGCGGGAATTCCCGCCGTCTTTTTTA
TGAACTTTTCGAAAGCTGCT

Product: O-acetylhomoserine/O-acetylserine sulfhydrylase

Products: NA

Alternate protein names: L-methioninase [H]

Number of amino acids: Translated: 448; Mature: 448

Protein sequence:

>448_residues
MRLRYFRAARRECMAVMIFKFHRIEDGMKTESLCLHAGYEPKNGEPRVVPIAQSTTFRYESTAAVARLFDLEDAGFFYTR
LGNPTVDAVERKIAALEGGVGALCTSSGQAANLLALLNVARSGDHIVSASSIYGGTFNLFAVTLKKLGIEVTFVDQTASD
EELEKAFRPNTKAVFGEVLTNPSMDVLDIERFAGLAHRHGLPLVVDSTFATPVLCRPFEYGADVVVHSTTKYMDGHALQM
GGVIVDSGNFDWTGGKFSEFTEPDPSYHGLVYTEAFGRAAYIVKARVQLMRDMGCCQSPQGAFYINQGLETLPLRMERHC
RNAETVAAYLANHPKVESVSYPRLPGNANKVLADKYLPDGCSGVISFSLGGGRDAGARFIDSLAMVSLEVHVADIRTCVL
HPASSTHRQLTDEQLREAGINPGTVRLSVGLEHVDDIIADLDQALKQA

Sequences:

>Translated_448_residues
MRLRYFRAARRECMAVMIFKFHRIEDGMKTESLCLHAGYEPKNGEPRVVPIAQSTTFRYESTAAVARLFDLEDAGFFYTR
LGNPTVDAVERKIAALEGGVGALCTSSGQAANLLALLNVARSGDHIVSASSIYGGTFNLFAVTLKKLGIEVTFVDQTASD
EELEKAFRPNTKAVFGEVLTNPSMDVLDIERFAGLAHRHGLPLVVDSTFATPVLCRPFEYGADVVVHSTTKYMDGHALQM
GGVIVDSGNFDWTGGKFSEFTEPDPSYHGLVYTEAFGRAAYIVKARVQLMRDMGCCQSPQGAFYINQGLETLPLRMERHC
RNAETVAAYLANHPKVESVSYPRLPGNANKVLADKYLPDGCSGVISFSLGGGRDAGARFIDSLAMVSLEVHVADIRTCVL
HPASSTHRQLTDEQLREAGINPGTVRLSVGLEHVDDIIADLDQALKQA
>Mature_448_residues
MRLRYFRAARRECMAVMIFKFHRIEDGMKTESLCLHAGYEPKNGEPRVVPIAQSTTFRYESTAAVARLFDLEDAGFFYTR
LGNPTVDAVERKIAALEGGVGALCTSSGQAANLLALLNVARSGDHIVSASSIYGGTFNLFAVTLKKLGIEVTFVDQTASD
EELEKAFRPNTKAVFGEVLTNPSMDVLDIERFAGLAHRHGLPLVVDSTFATPVLCRPFEYGADVVVHSTTKYMDGHALQM
GGVIVDSGNFDWTGGKFSEFTEPDPSYHGLVYTEAFGRAAYIVKARVQLMRDMGCCQSPQGAFYINQGLETLPLRMERHC
RNAETVAAYLANHPKVESVSYPRLPGNANKVLADKYLPDGCSGVISFSLGGGRDAGARFIDSLAMVSLEVHVADIRTCVL
HPASSTHRQLTDEQLREAGINPGTVRLSVGLEHVDDIIADLDQALKQA

Specific function: Methionine biosynthesis; second step. [C]

COG id: COG2873

COG function: function code E; O-acetylhomoserine sulfhydrylase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the trans-sulfuration enzymes family [H]

Homologues:

Organism=Homo sapiens, GI21361334, Length=419, Percent_Identity=33.6515513126492, Blast_Score=200, Evalue=2e-51,
Organism=Homo sapiens, GI299473759, Length=419, Percent_Identity=31.2649164677804, Blast_Score=167, Evalue=2e-41,
Organism=Homo sapiens, GI25453487, Length=418, Percent_Identity=28.9473684210526, Blast_Score=145, Evalue=8e-35,
Organism=Escherichia coli, GI1790375, Length=401, Percent_Identity=31.1720698254364, Blast_Score=183, Evalue=2e-47,
Organism=Escherichia coli, GI1789383, Length=372, Percent_Identity=27.6881720430108, Blast_Score=128, Evalue=7e-31,
Organism=Caenorhabditis elegans, GI71980708, Length=408, Percent_Identity=33.0882352941176, Blast_Score=222, Evalue=3e-58,
Organism=Caenorhabditis elegans, GI17560056, Length=420, Percent_Identity=35.4761904761905, Blast_Score=198, Evalue=4e-51,
Organism=Caenorhabditis elegans, GI17538055, Length=421, Percent_Identity=35.1543942992874, Blast_Score=195, Evalue=4e-50,
Organism=Saccharomyces cerevisiae, GI6323333, Length=432, Percent_Identity=43.5185185185185, Blast_Score=328, Evalue=1e-90,
Organism=Saccharomyces cerevisiae, GI6319307, Length=432, Percent_Identity=31.712962962963, Blast_Score=193, Evalue=4e-50,
Organism=Saccharomyces cerevisiae, GI6321170, Length=316, Percent_Identity=31.6455696202532, Blast_Score=126, Evalue=9e-30,
Organism=Saccharomyces cerevisiae, GI6321254, Length=447, Percent_Identity=25.0559284116331, Blast_Score=96, Evalue=1e-20,
Organism=Saccharomyces cerevisiae, GI6321904, Length=410, Percent_Identity=25.1219512195122, Blast_Score=83, Evalue=1e-16,
Organism=Drosophila melanogaster, GI19922554, Length=433, Percent_Identity=36.0277136258661, Blast_Score=221, Evalue=1e-57,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000277
- InterPro:   IPR006237
- InterPro:   IPR015424
- InterPro:   IPR015421
- InterPro:   IPR015422 [H]

Pfam domain/function: PF01053 Cys_Met_Meta_PP [H]

EC number: =4.4.1.11 [H]

Molecular weight: Translated: 48861; Mature: 48861

Theoretical pI: Translated: 6.28; Mature: 6.28

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.0 %Cys     (Translated Protein)
2.5 %Met     (Translated Protein)
4.5 %Cys+Met (Translated Protein)
2.0 %Cys     (Mature Protein)
2.5 %Met     (Mature Protein)
4.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRLRYFRAARRECMAVMIFKFHRIEDGMKTESLCLHAGYEPKNGEPRVVPIAQSTTFRYE
CCCHHHHHHHHHHHHHHHHHHHHHCCCCCCCCEEEECCCCCCCCCCCEEEEECCCEEEEH
STAAVARLFDLEDAGFFYTRLGNPTVDAVERKIAALEGGVGALCTSSGQAANLLALLNVA
HHHHHHHHHCCCCCCEEEEECCCCCHHHHHHHHHHHCCCCCEEECCCCCHHHHHHHHHHH
RSGDHIVSASSIYGGTFNLFAVTLKKLGIEVTFVDQTASDEELEKAFRPNTKAVFGEVLT
CCCCCEEEECCCCCCCHHHHHHHHHHHCEEEEEECCCCCHHHHHHHHCCCHHHHHHHHHC
NPSMDVLDIERFAGLAHRHGLPLVVDSTFATPVLCRPFEYGADVVVHSTTKYMDGHALQM
CCCCCCEEHHHHHHHHHHCCCCEEEECCCCCCEEECCCCCCCCEEEECCHHHCCCCEEEE
GGVIVDSGNFDWTGGKFSEFTEPDPSYHGLVYTEAFGRAAYIVKARVQLMRDMGCCQSPQ
CCEEEECCCCCCCCCCCCCCCCCCCCCCCEEEEECCCCHHHHHHHHHHHHHHHCCCCCCC
GAFYINQGLETLPLRMERHCRNAETVAAYLANHPKVESVSYPRLPGNANKVLADKYLPDG
CCEEECCCCHHHHHHHHHHCCCHHHHHHHHHCCCCCCCCCCCCCCCCCCCEEHHCCCCCC
CSGVISFSLGGGRDAGARFIDSLAMVSLEVHVADIRTCVLHPASSTHRQLTDEQLREAGI
CCEEEEEECCCCCCHHHHHHHHHHHHEEEEEHHHEEEEEECCCCHHHHHHHHHHHHHCCC
NPGTVRLSVGLEHVDDIIADLDQALKQA
CCCEEEEEECHHHHHHHHHHHHHHHHCC
>Mature Secondary Structure
MRLRYFRAARRECMAVMIFKFHRIEDGMKTESLCLHAGYEPKNGEPRVVPIAQSTTFRYE
CCCHHHHHHHHHHHHHHHHHHHHHCCCCCCCCEEEECCCCCCCCCCCEEEEECCCEEEEH
STAAVARLFDLEDAGFFYTRLGNPTVDAVERKIAALEGGVGALCTSSGQAANLLALLNVA
HHHHHHHHHCCCCCCEEEEECCCCCHHHHHHHHHHHCCCCCEEECCCCCHHHHHHHHHHH
RSGDHIVSASSIYGGTFNLFAVTLKKLGIEVTFVDQTASDEELEKAFRPNTKAVFGEVLT
CCCCCEEEECCCCCCCHHHHHHHHHHHCEEEEEECCCCCHHHHHHHHCCCHHHHHHHHHC
NPSMDVLDIERFAGLAHRHGLPLVVDSTFATPVLCRPFEYGADVVVHSTTKYMDGHALQM
CCCCCCEEHHHHHHHHHHCCCCEEEECCCCCCEEECCCCCCCCEEEECCHHHCCCCEEEE
GGVIVDSGNFDWTGGKFSEFTEPDPSYHGLVYTEAFGRAAYIVKARVQLMRDMGCCQSPQ
CCEEEECCCCCCCCCCCCCCCCCCCCCCCEEEEECCCCHHHHHHHHHHHHHHHCCCCCCC
GAFYINQGLETLPLRMERHCRNAETVAAYLANHPKVESVSYPRLPGNANKVLADKYLPDG
CCEEECCCCHHHHHHHHHHCCCHHHHHHHHHCCCCCCCCCCCCCCCCCCCEEHHCCCCCC
CSGVISFSLGGGRDAGARFIDSLAMVSLEVHVADIRTCVLHPASSTHRQLTDEQLREAGI
CCEEEEEECCCCCCHHHHHHHHHHHHEEEEEHHHEEEEEECCCCHHHHHHHHHHHHHCCC
NPGTVRLSVGLEHVDDIIADLDQALKQA
CCCEEEEEECHHHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8586629; 9190812; 3365412 [H]