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The map label for this gene is manX [H]

Identifier: 220904243

GI number: 220904243

Start: 1131573

End: 1132031

Strand: Reverse

Name: manX [H]

Synonym: Ddes_0970

Alternate gene names: 220904243

Gene position: 1132031-1131573 (Counterclockwise)

Preceding gene: 220904244

Following gene: 220904242

Centisome position: 39.4

GC content: 55.99

Gene sequence:

>459_bases
ATGTGGTTTCGCGTGGACAATCGCCTGATACACGGGCAGGTCATTGAGGCATGGCTGCCCTATACCGGTGCCAAGCATCT
GGTGGTCGCCAATGATGAGCTGGCGGCAGACATACTGCGCCAGCAGATTATTGAACTCGCCGTGCCGCAGCGCGTTCTTA
CCCACTTCATTCCCGTGGATGATCTGGCCTTCACTCTTGACGCCTGTGGCGAAGACAGTTTCGTACTTTTCGGCAACTGC
CGGGACGCCCGCCGCGCCAATGACGCAGGGGTGATTATGGAAACTCTCAATATCGGCAATCTGCATTACGGTCCTGAAAA
AACGCAGGTGCTCCCGCATGTGGCCCTGTCTGCCGAAGACAGGGAAGACCTGCGCTCCATGCAGCAACATCTGGTGCAAC
TGGATTTCCGCAGTGTGCCTACGGAAACCGTAAGGGGTTCCCATGAGCAGTTTATATGA

Upstream 100 bases:

>100_bases
GAAGAACTCGCCCAACTGGCGGGCGAGGCCGGATCCAAGGGTATTGTGGTCGCCGGAAACATGCTGCGCAACAAGGCGCG
AACCAAAAGCGACAGCTAGT

Downstream 100 bases:

>100_bases
AATGACGGCAGCAGGGGTTCCCTACGCTTTTTTTTTGCCCTGGCCGGTGCAGCCCGCTCTTCCTGTATAATCGGCCTTGT
TGACCGCCCCATCTGCCTTG

Product: PTS system sorbose subfamily IIB component

Products: NA

Alternate protein names: EIIAB-Man; Mannose-specific phosphotransferase enzyme IIA component; EIII-Man; PTS system mannose-specific EIIA component; Mannose-specific phosphotransferase enzyme IIB component; PTS system mannose-specific EIIB component [H]

Number of amino acids: Translated: 152; Mature: 152

Protein sequence:

>152_residues
MWFRVDNRLIHGQVIEAWLPYTGAKHLVVANDELAADILRQQIIELAVPQRVLTHFIPVDDLAFTLDACGEDSFVLFGNC
RDARRANDAGVIMETLNIGNLHYGPEKTQVLPHVALSAEDREDLRSMQQHLVQLDFRSVPTETVRGSHEQFI

Sequences:

>Translated_152_residues
MWFRVDNRLIHGQVIEAWLPYTGAKHLVVANDELAADILRQQIIELAVPQRVLTHFIPVDDLAFTLDACGEDSFVLFGNC
RDARRANDAGVIMETLNIGNLHYGPEKTQVLPHVALSAEDREDLRSMQQHLVQLDFRSVPTETVRGSHEQFI
>Mature_152_residues
MWFRVDNRLIHGQVIEAWLPYTGAKHLVVANDELAADILRQQIIELAVPQRVLTHFIPVDDLAFTLDACGEDSFVLFGNC
RDARRANDAGVIMETLNIGNLHYGPEKTQVLPHVALSAEDREDLRSMQQHLVQLDFRSVPTETVRGSHEQFI

Specific function: The phosphoenolpyruvate-dependent sugar phosphotransferase system (sugar PTS), a major carbohydrate active -transport system, catalyzes the phosphorylation of incoming sugar substrates concomitantly with their translocation across the cell membrane. This

COG id: COG3444

COG function: function code G; Phosphotransferase system, mannose/fructose/N-acetylgalactosamine-specific component IIB

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 PTS EIIB type-4 domain [H]

Homologues:

Organism=Escherichia coli, GI1788120, Length=142, Percent_Identity=30.9859154929577, Blast_Score=82, Evalue=2e-17,
Organism=Escherichia coli, GI87082217, Length=142, Percent_Identity=29.5774647887324, Blast_Score=71, Evalue=3e-14,

Paralogues:

None

Copy number: 220 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 2880 Molecules/Cell In: Stationary Phase, Rich Media (Based on E. coli). 260 Molecules/Cell In: Stationary Phase, Rich Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004701
- InterPro:   IPR004720 [H]

Pfam domain/function: PF03610 EIIA-man; PF03830 PTSIIB_sorb [H]

EC number: =2.7.1.69 [H]

Molecular weight: Translated: 17228; Mature: 17228

Theoretical pI: Translated: 4.96; Mature: 4.96

Prosite motif: PS51101 PTS_EIIB_TYPE_4 ; PS00133 CARBOXYPEPT_ZN_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.3 %Cys     (Translated Protein)
2.0 %Met     (Translated Protein)
3.3 %Cys+Met (Translated Protein)
1.3 %Cys     (Mature Protein)
2.0 %Met     (Mature Protein)
3.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MWFRVDNRLIHGQVIEAWLPYTGAKHLVVANDELAADILRQQIIELAVPQRVLTHFIPVD
CEEECCCEEEHHHHHHHHCCCCCCCEEEEECCHHHHHHHHHHHHHHHHHHHHHHHHCCHH
DLAFTLDACGEDSFVLFGNCRDARRANDAGVIMETLNIGNLHYGPEKTQVLPHVALSAED
HHHHHHHCCCCCCEEEEECCCCCCCCCCCCEEEEEECCCCCCCCCCCHHHCCHHCCCCCC
REDLRSMQQHLVQLDFRSVPTETVRGSHEQFI
HHHHHHHHHHHHHHHHCCCCHHHHCCCCCCCC
>Mature Secondary Structure
MWFRVDNRLIHGQVIEAWLPYTGAKHLVVANDELAADILRQQIIELAVPQRVLTHFIPVD
CEEECCCEEEHHHHHHHHCCCCCCCEEEEECCHHHHHHHHHHHHHHHHHHHHHHHHCCHH
DLAFTLDACGEDSFVLFGNCRDARRANDAGVIMETLNIGNLHYGPEKTQVLPHVALSAED
HHHHHHHCCCCCCEEEEECCCCCCCCCCCCEEEEEECCCCCCCCCCCHHHCCHHCCCCCC
REDLRSMQQHLVQLDFRSVPTETVRGSHEQFI
HHHHHHHHHHHHHHHHCCCCHHHHCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA