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The map label for this gene is hyaD [H]

Identifier: 220904108

GI number: 220904108

Start: 980215

End: 980748

Strand: Reverse

Name: hyaD [H]

Synonym: Ddes_0834

Alternate gene names: 220904108

Gene position: 980748-980215 (Counterclockwise)

Preceding gene: 220904109

Following gene: 220904103

Centisome position: 34.13

GC content: 61.24

Gene sequence:

>534_bases
ATGGAAGCGGTAATTATCATCGGCCTCGGCAATATTCTTTACGGCGACGAAGGCTACGGCGTCAGGCTGGCGCAAAATCT
CTATTCCCGCTGGGATTTTCCCGGCAATGTAGAAGTGGTGGACGGCGGCACACAAGGCCAGACGCTGCTGACCTATGTGG
AACGCGCGGACAGGCTGCTGGTACTGGATGCAGTGGACTTTGGCCTTGAACCGGGGGAGATGGTGCTGCGTGAACAGATG
CCCGCCTATCTTACCGCACAAAAAATCGGACCGCACCAGAACAGTTTTTCCGAGGTCCTTGGTCTGGCGACCCTGCGCGG
CAACATGCCGGGGCACTGTGCGCTTATCGGCGTGCAGCCCGCGGCCATGACCCTGGGCGCGGCGCTTTCCCCGGCGGTGG
CCTGCTGCATGGATAAAGGAGAGGCCATGGCGCTGGACGTTTTGCGCCAATGGGGCGTGAACCCCGCGCGGCGCGATGAA
CCCCGTCATTTATCCGCATCTGCGCTGGATGCCCTGCTGCTGGGGGAACTGTGA

Upstream 100 bases:

>100_bases
TGCCCCAGGACCGCATCAGACGCAACAACAGGGCGGCTTCACTGCAGTGGGGCCGCCTTCTGCTTGCAGGCCCGGCCTGC
CTACAAAAACAGGATATCTC

Downstream 100 bases:

>100_bases
GCGGTTCAAACCTGTGGGGGGGCCTTTTGCAAAAGGTTCCCCCCCGCAGGCCAAAAACCGGTATCAGGTTTCACTCAGTT
GCAAAAATTTTCCTGCCCCG

Product: hydrogenase maturation protease

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 177; Mature: 177

Protein sequence:

>177_residues
MEAVIIIGLGNILYGDEGYGVRLAQNLYSRWDFPGNVEVVDGGTQGQTLLTYVERADRLLVLDAVDFGLEPGEMVLREQM
PAYLTAQKIGPHQNSFSEVLGLATLRGNMPGHCALIGVQPAAMTLGAALSPAVACCMDKGEAMALDVLRQWGVNPARRDE
PRHLSASALDALLLGEL

Sequences:

>Translated_177_residues
MEAVIIIGLGNILYGDEGYGVRLAQNLYSRWDFPGNVEVVDGGTQGQTLLTYVERADRLLVLDAVDFGLEPGEMVLREQM
PAYLTAQKIGPHQNSFSEVLGLATLRGNMPGHCALIGVQPAAMTLGAALSPAVACCMDKGEAMALDVLRQWGVNPARRDE
PRHLSASALDALLLGEL
>Mature_177_residues
MEAVIIIGLGNILYGDEGYGVRLAQNLYSRWDFPGNVEVVDGGTQGQTLLTYVERADRLLVLDAVDFGLEPGEMVLREQM
PAYLTAQKIGPHQNSFSEVLGLATLRGNMPGHCALIGVQPAAMTLGAALSPAVACCMDKGEAMALDVLRQWGVNPARRDE
PRHLSASALDALLLGEL

Specific function: Protease involved in the C-terminal processing of hyaB, the large subunit of hydrogenase 1 (Potential) [H]

COG id: COG0680

COG function: function code C; Ni,Fe-hydrogenase maturation factor

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the peptidase A31 family [H]

Homologues:

Organism=Escherichia coli, GI1787209, Length=172, Percent_Identity=48.8372093023256, Blast_Score=155, Evalue=1e-39,
Organism=Escherichia coli, GI1789367, Length=159, Percent_Identity=36.4779874213836, Blast_Score=94, Evalue=3e-21,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004419
- InterPro:   IPR000671 [H]

Pfam domain/function: PF01750 HycI [H]

EC number: 3.4.24.- [C]

Molecular weight: Translated: 18961; Mature: 18961

Theoretical pI: Translated: 4.42; Mature: 4.42

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.7 %Cys     (Translated Protein)
4.0 %Met     (Translated Protein)
5.6 %Cys+Met (Translated Protein)
1.7 %Cys     (Mature Protein)
4.0 %Met     (Mature Protein)
5.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MEAVIIIGLGNILYGDEGYGVRLAQNLYSRWDFPGNVEVVDGGTQGQTLLTYVERADRLL
CCEEEEEECCCEEECCCCCCHHHHHHHHHHCCCCCCEEEEECCCCCHHHHHHHHHCCCEE
VLDAVDFGLEPGEMVLREQMPAYLTAQKIGPHQNSFSEVLGLATLRGNMPGHCALIGVQP
EEEEHHCCCCCHHHHHHHHCCHHEEHHHCCCCCCHHHHHHHHHHHCCCCCCCEEEEECCH
AAMTLGAALSPAVACCMDKGEAMALDVLRQWGVNPARRDEPRHLSASALDALLLGEL
HHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHCCCCCCCCCCCCCCHHHHHHHHHCCC
>Mature Secondary Structure
MEAVIIIGLGNILYGDEGYGVRLAQNLYSRWDFPGNVEVVDGGTQGQTLLTYVERADRLL
CCEEEEEECCCEEECCCCCCHHHHHHHHHHCCCCCCEEEEECCCCCHHHHHHHHHCCCEE
VLDAVDFGLEPGEMVLREQMPAYLTAQKIGPHQNSFSEVLGLATLRGNMPGHCALIGVQP
EEEEHHCCCCCHHHHHHHHCCHHEEHHHCCCCCCHHHHHHHHHHHCCCCCCCEEEEECCH
AAMTLGAALSPAVACCMDKGEAMALDVLRQWGVNPARRDEPRHLSASALDALLLGEL
HHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHCCCCCCCCCCCCCCHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: Ni [C]

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 2180913; 8905232; 9278503 [H]