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The map label for this gene is fdxH [H]

Identifier: 220904100

GI number: 220904100

Start: 968438

End: 969310

Strand: Reverse

Name: fdxH [H]

Synonym: Ddes_0826

Alternate gene names: 220904100

Gene position: 969310-968438 (Counterclockwise)

Preceding gene: 220904101

Following gene: 220904099

Centisome position: 33.73

GC content: 61.05

Gene sequence:

>873_bases
ATGATAACTGAGACGCGCCTTCCCCAAAAACCCCGCAGCTTTGCACAGCGCCTGCTTTACCCTTTCAGACGCGGCACGGG
ACCGGCGATGCAGGAGCCGGTGGGCTTTTTTACCGATACCTCGGTATGTATCGGCTGCAAGGCCTGCCAGGTGGGCTGCA
AGCACTGGAACATGCTGCCAGGCCTTGAACCACGACTTTCTGGCCGCAGCTACGACAACACCCTGGAACTTTCCGCCCGC
ACATGGCGGCATGTAAAGTTCGCCGAGGTCATTGATACCGATATTCCGGCCAATAACCGTCAGGGCATGCACTGGCTTCT
CGCCAGCGACAGTTGCAAGCACTGCGACGATGCCCCCTGCATGCGGGCCTGCCCCACGGGGGCGCTTGTCCGCACTGAAG
TGGGCGGCGTGTACCCGCAGGCAGACATCTGCAACGGCTGTGCCTCATGCGTTGCGGCCTGCCCCTTTGGCGTTATTGCC
AGAAATGAGAAAAGCGGCCACTCGCACAAGTGCACCTTCTGCCTTGACCGGGTGCGTGACGGCCTGCAGCCGGCTTGCGC
CAAAACCTGCCCCGCTGCTTCCATACGCTTCGGCAGGCTTGAAGACATGCGCAATGCCGCCCGCAACCGCCTGGCCCACC
TGCGGGCACAGGGTGTGAGCAATGCCCGCCTTTACGGCCTTGAACCCACAGAAAACTATTCGTCACTGCACAATATTTTC
CTGCTGCTGGACAGGCCCTCCACCTATGGCCTGCCCGAAACTCCCCTGCATCCCGCCCGCCGTCTCAAGGGAGACTATAT
CCGCGCTGCCGCCGGAGTGGTTCTCGGCATTGCCATACTGGGCGCGTCAGTCATTTTCGGAGGTGTCCTGTAA

Upstream 100 bases:

>100_bases
AGCGAACACAAGGGCGACTTCAAGGATGCCTACGCGCCTCTGCCCGACGCTCCCATGAGCAAGGTCCCCTGGATATGCAA
GCCCGAAGGGAGGCTCGACT

Downstream 100 bases:

>100_bases
TGCAAAATACCGCGCAAAACACACGAGGCGCTCTGCCGCCAAACACCTCCCGGTCCTACAGCCAGGATTATTCCGGCGAT
ATCATCCCCCACCTGCCCCC

Product: 4Fe-4S ferredoxin iron-sulfur binding domain-containing protein

Products: CO2; NADH

Alternate protein names: Formate dehydrogenase subunit beta; FDH subunit beta [H]

Number of amino acids: Translated: 290; Mature: 290

Protein sequence:

>290_residues
MITETRLPQKPRSFAQRLLYPFRRGTGPAMQEPVGFFTDTSVCIGCKACQVGCKHWNMLPGLEPRLSGRSYDNTLELSAR
TWRHVKFAEVIDTDIPANNRQGMHWLLASDSCKHCDDAPCMRACPTGALVRTEVGGVYPQADICNGCASCVAACPFGVIA
RNEKSGHSHKCTFCLDRVRDGLQPACAKTCPAASIRFGRLEDMRNAARNRLAHLRAQGVSNARLYGLEPTENYSSLHNIF
LLLDRPSTYGLPETPLHPARRLKGDYIRAAAGVVLGIAILGASVIFGGVL

Sequences:

>Translated_290_residues
MITETRLPQKPRSFAQRLLYPFRRGTGPAMQEPVGFFTDTSVCIGCKACQVGCKHWNMLPGLEPRLSGRSYDNTLELSAR
TWRHVKFAEVIDTDIPANNRQGMHWLLASDSCKHCDDAPCMRACPTGALVRTEVGGVYPQADICNGCASCVAACPFGVIA
RNEKSGHSHKCTFCLDRVRDGLQPACAKTCPAASIRFGRLEDMRNAARNRLAHLRAQGVSNARLYGLEPTENYSSLHNIF
LLLDRPSTYGLPETPLHPARRLKGDYIRAAAGVVLGIAILGASVIFGGVL
>Mature_290_residues
MITETRLPQKPRSFAQRLLYPFRRGTGPAMQEPVGFFTDTSVCIGCKACQVGCKHWNMLPGLEPRLSGRSYDNTLELSAR
TWRHVKFAEVIDTDIPANNRQGMHWLLASDSCKHCDDAPCMRACPTGALVRTEVGGVYPQADICNGCASCVAACPFGVIA
RNEKSGHSHKCTFCLDRVRDGLQPACAKTCPAASIRFGRLEDMRNAARNRLAHLRAQGVSNARLYGLEPTENYSSLHNIF
LLLDRPSTYGLPETPLHPARRLKGDYIRAAAGVVLGIAILGASVIFGGVL

Specific function: Allows to use formate as major electron donor during aerobic respiration. The beta chain is an electron transfer unit containing 4 cysteine clusters involved in the formation of iron- sulfur centers. Electrons are transferred from the gamma chain to the m

COG id: COG0437

COG function: function code C; Fe-S-cluster-containing hydrogenase components 1

Gene ontology:

Cell location: Cell membrane [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 4 4Fe-4S ferredoxin-type domains [H]

Homologues:

Organism=Escherichia coli, GI1790326, Length=263, Percent_Identity=38.7832699619772, Blast_Score=171, Evalue=8e-44,
Organism=Escherichia coli, GI1787749, Length=226, Percent_Identity=38.4955752212389, Blast_Score=162, Evalue=2e-41,
Organism=Escherichia coli, GI1787872, Length=176, Percent_Identity=35.7954545454545, Blast_Score=113, Evalue=1e-26,
Organism=Escherichia coli, GI1787122, Length=176, Percent_Identity=35.2272727272727, Blast_Score=113, Evalue=1e-26,
Organism=Escherichia coli, GI1789370, Length=193, Percent_Identity=30.5699481865285, Blast_Score=94, Evalue=1e-20,
Organism=Escherichia coli, GI226510944, Length=191, Percent_Identity=31.9371727748691, Blast_Score=92, Evalue=6e-20,
Organism=Escherichia coli, GI2367345, Length=170, Percent_Identity=32.3529411764706, Blast_Score=91, Evalue=6e-20,
Organism=Escherichia coli, GI87082179, Length=181, Percent_Identity=28.7292817679558, Blast_Score=70, Evalue=1e-13,
Organism=Escherichia coli, GI1787740, Length=87, Percent_Identity=40.2298850574713, Blast_Score=70, Evalue=2e-13,
Organism=Escherichia coli, GI1787478, Length=87, Percent_Identity=40.2298850574713, Blast_Score=69, Evalue=3e-13,
Organism=Escherichia coli, GI1788811, Length=182, Percent_Identity=26.3736263736264, Blast_Score=69, Evalue=3e-13,
Organism=Escherichia coli, GI1789067, Length=195, Percent_Identity=29.2307692307692, Blast_Score=64, Evalue=8e-12,
Organism=Escherichia coli, GI2367245, Length=178, Percent_Identity=28.0898876404494, Blast_Score=64, Evalue=1e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR017896
- InterPro:   IPR017900
- InterPro:   IPR006470
- InterPro:   IPR014603
- InterPro:   IPR015246 [H]

Pfam domain/function: PF09163 Form-deh_trans [H]

EC number: 1.2.1.2

Molecular weight: Translated: 31700; Mature: 31700

Theoretical pI: Translated: 8.73; Mature: 8.73

Prosite motif: PS00198 4FE4S_FERREDOXIN

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

5.5 %Cys     (Translated Protein)
2.1 %Met     (Translated Protein)
7.6 %Cys+Met (Translated Protein)
5.5 %Cys     (Mature Protein)
2.1 %Met     (Mature Protein)
7.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MITETRLPQKPRSFAQRLLYPFRRGTGPAMQEPVGFFTDTSVCIGCKACQVGCKHWNMLP
CCCCCCCCCCHHHHHHHHHHHHHCCCCCCHHCCCCCCCCCHHHHHHHHHHHHHHHCCCCC
GLEPRLSGRSYDNTLELSARTWRHVKFAEVIDTDIPANNRQGMHWLLASDSCKHCDDAPC
CCCCCCCCCCCCCEEEECHHHHHHHHHHHHHHCCCCCCCCCCEEEEEECCCCCCCCCCCH
MRACPTGALVRTEVGGVYPQADICNGCASCVAACPFGVIARNEKSGHSHKCTFCLDRVRD
HHHCCCCCEEEHHCCCCCCCHHHHHHHHHHHHHCCCCCEECCCCCCCCCHHHHHHHHHHH
GLQPACAKTCPAASIRFGRLEDMRNAARNRLAHLRAQGVSNARLYGLEPTENYSSLHNIF
CCCHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHCCCCCCEEEECCCCHHHHHHCEEE
LLLDRPSTYGLPETPLHPARRLKGDYIRAAAGVVLGIAILGASVIFGGVL
EEEECCCCCCCCCCCCCHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHCCC
>Mature Secondary Structure
MITETRLPQKPRSFAQRLLYPFRRGTGPAMQEPVGFFTDTSVCIGCKACQVGCKHWNMLP
CCCCCCCCCCHHHHHHHHHHHHHCCCCCCHHCCCCCCCCCHHHHHHHHHHHHHHHCCCCC
GLEPRLSGRSYDNTLELSARTWRHVKFAEVIDTDIPANNRQGMHWLLASDSCKHCDDAPC
CCCCCCCCCCCCCEEEECHHHHHHHHHHHHHHCCCCCCCCCCEEEEEECCCCCCCCCCCH
MRACPTGALVRTEVGGVYPQADICNGCASCVAACPFGVIARNEKSGHSHKCTFCLDRVRD
HHHCCCCCEEEHHCCCCCCCHHHHHHHHHHHHHCCCCCEECCCCCCCCCHHHHHHHHHHH
GLQPACAKTCPAASIRFGRLEDMRNAARNRLAHLRAQGVSNARLYGLEPTENYSSLHNIF
CCCHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHCCCCCCEEEECCCCHHHHHHCEEE
LLLDRPSTYGLPETPLHPARRLKGDYIRAAAGVVLGIAILGASVIFGGVL
EEEECCCCCCCCCCCCCHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: formate; NAD+

Specific reaction: formate + NAD+ = CO2 + NADH

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: 7542800 [H]