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The map label for this gene is opuAB [H]

Identifier: 220904097

GI number: 220904097

Start: 964357

End: 965199

Strand: Reverse

Name: opuAB [H]

Synonym: Ddes_0823

Alternate gene names: 220904097

Gene position: 965199-964357 (Counterclockwise)

Preceding gene: 220904098

Following gene: 220904096

Centisome position: 33.59

GC content: 60.5

Gene sequence:

>843_bases
ATGCTGCCTAGACTCCCCTTGGCCGGTTATATTGACAGCGGCGTGGAGTTTCTGGTGGAGCAGTTTTCCGGCGTTACCCG
CGCCGGGTCCGCAATCATGCTTGCCCTGCTGGACCGTTTTGAAGAATTTCTGCTGCTGCCGCCCCCGTGGCTCTTTATTC
TGCTTCTGGCAGCTCTGGCCTGGTGGGTAACGCGCCGCCCGGGACTGCCCGTATTCGTGGCTCTGGGCTTTGCCCTTCTG
TGGAACCTGGGGCTGTGGACGCCCACCATCAGCACTCTGGCCCTGGTGCTTTCGGCCACCCTGCTTTCCGTGCTGGTAGG
CGTACCCTGCGGCATTCTTGCGGCCATGAGCCCTGTGGCACGCAAAATTGTCATGCCCGTGCTGGACGTCATGCAGACCA
TGCCAGCCTTTGTGTATCTTATTCCGGCCATTCCCTTTTTCGGCATCGGCAAGGTCAGCGCGGTGGTAGCCACGGTCATC
TTTTCAGTGCCTCCGGCCATACGCTTTACCTGCCTGGGCATACAGCAGGTTCCCCGCGATCTTGTGGAGTGCACCGAGGC
CTTTGGCGCAACCCGCATGCAGCGTCTGTATAAGCTGGAACTGCCCCTGGCCATGCCCACCATTGTCGCAGGCGTAAACC
AGACAATCATGCTGGCCCTTTCAATGGCCGTCATCGCGGCCATGATCGGCGCGCGCGGCCTGGGCGGCGAGGTATGGAAG
GCCATTCAGCGCCTCAACATCGGCATGGGCTTTGAAGCCGGGCTTGGCATCGTTATTGTCGCCATTACCCTTGACCGCCT
CTTCCGTGCGCTGGCGCAAAAAAGTTCCGGCAAGGCCCATTAG

Upstream 100 bases:

>100_bases
CCGTTGGCTGTGGTAGATAACCGCCAGCGTTTACAGGGTGTTATCGTGCGTGGACTGTTACTGGGCGCTCTGGTGGAACA
TAACGACAGGAGAGGCGCGC

Downstream 100 bases:

>100_bases
GCCGGACTGATTTTGAGAATACGCATTCTCAAAATTTCAGGGCGCTCAATTTGGATTGCCCCCATACCGTGCAGCCGCGG
TTTGTTTGCAAACCTTGCAG

Product: binding-protein-dependent transport systems inner membrane component

Products: ADP; phosphate; L-proline [Cytoplasm] [C]

Alternate protein names: NA

Number of amino acids: Translated: 280; Mature: 280

Protein sequence:

>280_residues
MLPRLPLAGYIDSGVEFLVEQFSGVTRAGSAIMLALLDRFEEFLLLPPPWLFILLLAALAWWVTRRPGLPVFVALGFALL
WNLGLWTPTISTLALVLSATLLSVLVGVPCGILAAMSPVARKIVMPVLDVMQTMPAFVYLIPAIPFFGIGKVSAVVATVI
FSVPPAIRFTCLGIQQVPRDLVECTEAFGATRMQRLYKLELPLAMPTIVAGVNQTIMLALSMAVIAAMIGARGLGGEVWK
AIQRLNIGMGFEAGLGIVIVAITLDRLFRALAQKSSGKAH

Sequences:

>Translated_280_residues
MLPRLPLAGYIDSGVEFLVEQFSGVTRAGSAIMLALLDRFEEFLLLPPPWLFILLLAALAWWVTRRPGLPVFVALGFALL
WNLGLWTPTISTLALVLSATLLSVLVGVPCGILAAMSPVARKIVMPVLDVMQTMPAFVYLIPAIPFFGIGKVSAVVATVI
FSVPPAIRFTCLGIQQVPRDLVECTEAFGATRMQRLYKLELPLAMPTIVAGVNQTIMLALSMAVIAAMIGARGLGGEVWK
AIQRLNIGMGFEAGLGIVIVAITLDRLFRALAQKSSGKAH
>Mature_280_residues
MLPRLPLAGYIDSGVEFLVEQFSGVTRAGSAIMLALLDRFEEFLLLPPPWLFILLLAALAWWVTRRPGLPVFVALGFALL
WNLGLWTPTISTLALVLSATLLSVLVGVPCGILAAMSPVARKIVMPVLDVMQTMPAFVYLIPAIPFFGIGKVSAVVATVI
FSVPPAIRFTCLGIQQVPRDLVECTEAFGATRMQRLYKLELPLAMPTIVAGVNQTIMLALSMAVIAAMIGARGLGGEVWK
AIQRLNIGMGFEAGLGIVIVAITLDRLFRALAQKSSGKAH

Specific function: Involved in a multicomponent binding-protein-dependent transport system for glycine betaine; probably responsible for the translocation of the substrate across the membrane [H]

COG id: COG4176

COG function: function code E; ABC-type proline/glycine betaine transport system, permease component

Gene ontology:

Cell location: Cell membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 ABC transmembrane type-1 domain [H]

Homologues:

Organism=Escherichia coli, GI1789033, Length=276, Percent_Identity=45.6521739130435, Blast_Score=211, Evalue=5e-56,
Organism=Escherichia coli, GI1788449, Length=240, Percent_Identity=30.4166666666667, Blast_Score=92, Evalue=5e-20,
Organism=Escherichia coli, GI1788451, Length=195, Percent_Identity=29.7435897435897, Blast_Score=89, Evalue=3e-19,
Organism=Escherichia coli, GI1786564, Length=152, Percent_Identity=30.2631578947368, Blast_Score=72, Evalue=5e-14,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000515 [H]

Pfam domain/function: PF00528 BPD_transp_1 [H]

EC number: NA

Molecular weight: Translated: 30080; Mature: 30080

Theoretical pI: Translated: 9.98; Mature: 9.98

Prosite motif: PS50928 ABC_TM1

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
4.3 %Met     (Translated Protein)
5.4 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
4.3 %Met     (Mature Protein)
5.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLPRLPLAGYIDSGVEFLVEQFSGVTRAGSAIMLALLDRFEEFLLLPPPWLFILLLAALA
CCCCCCCHHHHHHHHHHHHHHHHCHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHH
WWVTRRPGLPVFVALGFALLWNLGLWTPTISTLALVLSATLLSVLVGVPCGILAAMSPVA
HHHHCCCCCHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHH
RKIVMPVLDVMQTMPAFVYLIPAIPFFGIGKVSAVVATVIFSVPPAIRFTCLGIQQVPRD
HHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHH
LVECTEAFGATRMQRLYKLELPLAMPTIVAGVNQTIMLALSMAVIAAMIGARGLGGEVWK
HHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHCCHHHHHHHHHHHHHHHHCCCCCCHHHHH
AIQRLNIGMGFEAGLGIVIVAITLDRLFRALAQKSSGKAH
HHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHHHCCCCCCC
>Mature Secondary Structure
MLPRLPLAGYIDSGVEFLVEQFSGVTRAGSAIMLALLDRFEEFLLLPPPWLFILLLAALA
CCCCCCCHHHHHHHHHHHHHHHHCHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHH
WWVTRRPGLPVFVALGFALLWNLGLWTPTISTLALVLSATLLSVLVGVPCGILAAMSPVA
HHHHCCCCCHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHH
RKIVMPVLDVMQTMPAFVYLIPAIPFFGIGKVSAVVATVIFSVPPAIRFTCLGIQQVPRD
HHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHH
LVECTEAFGATRMQRLYKLELPLAMPTIVAGVNQTIMLALSMAVIAAMIGARGLGGEVWK
HHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHCCHHHHHHHHHHHHHHHHCCCCCCHHHHH
AIQRLNIGMGFEAGLGIVIVAITLDRLFRALAQKSSGKAH
HHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHHHCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: ATP; L-proline [Periplasm]; H2O [C]

Specific reaction: ATP + L-proline [Periplasm] + H2O = ADP + phosphate + L-proline [Cytoplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: 7622480; 8969502; 9384377 [H]