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The map label for this gene is nadB [H]

Identifier: 220904080

GI number: 220904080

Start: 944070

End: 945695

Strand: Reverse

Name: nadB [H]

Synonym: Ddes_0806

Alternate gene names: 220904080

Gene position: 945695-944070 (Counterclockwise)

Preceding gene: 220904081

Following gene: 220904079

Centisome position: 32.91

GC content: 61.99

Gene sequence:

>1626_bases
GTGAATGTCAGTCGCCGTCATGTGCCCGTATTAATTATTGGTTCGGGCATTGCCGGATGCACCGCCGCCCTTACGCTGGC
CGATGCCGGTCATGAAGTGCTTCTTATCAACGCCGGGAGCCAGTTGCTCGACGGCAACTCCGAACTGGCCCAGGGGGGCA
TCATCTATCGTGCCGACCCCGCCAGAAACGGCGGGCCGGGAACCGCCGGAGGCCAGAACAGCGATGACGCCGCTGCCCTG
GAAAAAGATATCCTTGTAGCAGGACACAATTACAACTATCGCAAAGCCGTACGCTTTCTCTGCCGTCAGGGGCCTTCCTG
CGTTGACGACATGCTCATCGAGCGCGCCAGGGTTCCCTTTGACCGCAATGAAGACGGCAGCTTTAACCTGACCCGCGAGG
GCGGGCATTCCACACCGCGCATCCTGCATTGCCGCGATTACTCGGGCCGGGCCATCATGGAAGGCCTTACAGCCCAGATC
ATGGTCCACCCGCGCATCACGCGCCTGCACCACCGCGCCGCCATTGACCTTCTGACAAGCCGCCACCACGCCAGGACCTC
ACAATACCGTTACGAGATCAACAATCGCTGCATGGGCGCGTACGTCCTGAACGAGGAAAGCGGCGAACCGGAAACCATCC
TGGCGGACTGGACGGTGCTTGCCACGGGCGGCGTGGGGCAGGTATTCCTGCATTCCACCAATGCCCCGGGCTGCGTGGGT
ACAGGCGTTTCCATGGCGTTTCGCGCCGGTGTGGATCTGGCAAATCTGGAATTCATGCAGTTTCACCCCACAGCCCTTTA
TGAAGAACGCAGCAACCGCCGCTCGCTCATCACCGAGGCCATGCGCGGCGAAGGCGCGCGCCTGCTCAACCACAAGGGGC
AAGCCTTCATGCAGGGTTATGACCCGCGCGGCGACCTGGCCCCGCGTGATGTGGTGGCCCAGGCCATGATGGACGAAATG
CTGCATAACGGTGCGCCCTGCCTTTTTCTGGACGTGAGCGGTGTGGAACAGGACCTGCCCACACGTTTTCCCACGGTATT
CCAGAAGTGCCTTGAAGCCGGCATCGACATCCGCCGCGAGCCCATCCCCGTTGTTCCCGCCGCCCACTATTTCTGCGGCG
GTGTGCTTACCGACGAGCGAGGGCGTACATCCATGCGCGGTCTTTACGCCATTGGCGAGTGCGCCTGCACCGGGCTGCAC
GGGGCCAACCGCCTGGCGAGTACATCCCTGCTCGAGGCCCTGGTGTGGGGTGTCAGCTGCGGCAAAGACCTGAACCGCCG
CCTCAAGGCCGAAAGCGGCCTGCCCAAGGGCCTGGCCGCCGCCATTCCCGACTGGCTGCACGAAGGGGACGAACGGCGCG
ATGACCCGGCCCTGGTGGCTCAGGACTGGACCAACATACGCAATACCATGTGGAACTATGTGGGCATTTCGCGCACAGAG
GCCCGGCTGCGCAGGGCGTTTGAAGACATGCGCGACCTTGTGCGGCATATCCACGACTTTTATAAATATACCCGCATTTC
ACGACGCCTTGTGGACCTTTTTCACGGCTCACAGACAGCCTACGTCATCATTCAGGCGGCCATGCGCAACCGCGCCAGCC
TGGGTTGTCACCACAGGGTGGATTGA

Upstream 100 bases:

>100_bases
CAAAAAGCCACCCCTCTGGCGATCGAGGAGGAACTCTGCCCCCCCGCCCGCCTCTCGCTAACCCGCATGCTTGAAGTATG
CGGCCAATGAGGTTTGTGCC

Downstream 100 bases:

>100_bases
CGCGGCGGTCCGCACAGGCAAGCCCGCTGGCGAAAAAACGAGGCCACCCGGCACGTGCGCCATGCCAAACACATAAAGGA
AATACCTGCTTATGAAAAAG

Product: L-aspartate oxidase

Products: NA

Alternate protein names: LASPO; Quinolinate synthase B [H]

Number of amino acids: Translated: 541; Mature: 541

Protein sequence:

>541_residues
MNVSRRHVPVLIIGSGIAGCTAALTLADAGHEVLLINAGSQLLDGNSELAQGGIIYRADPARNGGPGTAGGQNSDDAAAL
EKDILVAGHNYNYRKAVRFLCRQGPSCVDDMLIERARVPFDRNEDGSFNLTREGGHSTPRILHCRDYSGRAIMEGLTAQI
MVHPRITRLHHRAAIDLLTSRHHARTSQYRYEINNRCMGAYVLNEESGEPETILADWTVLATGGVGQVFLHSTNAPGCVG
TGVSMAFRAGVDLANLEFMQFHPTALYEERSNRRSLITEAMRGEGARLLNHKGQAFMQGYDPRGDLAPRDVVAQAMMDEM
LHNGAPCLFLDVSGVEQDLPTRFPTVFQKCLEAGIDIRREPIPVVPAAHYFCGGVLTDERGRTSMRGLYAIGECACTGLH
GANRLASTSLLEALVWGVSCGKDLNRRLKAESGLPKGLAAAIPDWLHEGDERRDDPALVAQDWTNIRNTMWNYVGISRTE
ARLRRAFEDMRDLVRHIHDFYKYTRISRRLVDLFHGSQTAYVIIQAAMRNRASLGCHHRVD

Sequences:

>Translated_541_residues
MNVSRRHVPVLIIGSGIAGCTAALTLADAGHEVLLINAGSQLLDGNSELAQGGIIYRADPARNGGPGTAGGQNSDDAAAL
EKDILVAGHNYNYRKAVRFLCRQGPSCVDDMLIERARVPFDRNEDGSFNLTREGGHSTPRILHCRDYSGRAIMEGLTAQI
MVHPRITRLHHRAAIDLLTSRHHARTSQYRYEINNRCMGAYVLNEESGEPETILADWTVLATGGVGQVFLHSTNAPGCVG
TGVSMAFRAGVDLANLEFMQFHPTALYEERSNRRSLITEAMRGEGARLLNHKGQAFMQGYDPRGDLAPRDVVAQAMMDEM
LHNGAPCLFLDVSGVEQDLPTRFPTVFQKCLEAGIDIRREPIPVVPAAHYFCGGVLTDERGRTSMRGLYAIGECACTGLH
GANRLASTSLLEALVWGVSCGKDLNRRLKAESGLPKGLAAAIPDWLHEGDERRDDPALVAQDWTNIRNTMWNYVGISRTE
ARLRRAFEDMRDLVRHIHDFYKYTRISRRLVDLFHGSQTAYVIIQAAMRNRASLGCHHRVD
>Mature_541_residues
MNVSRRHVPVLIIGSGIAGCTAALTLADAGHEVLLINAGSQLLDGNSELAQGGIIYRADPARNGGPGTAGGQNSDDAAAL
EKDILVAGHNYNYRKAVRFLCRQGPSCVDDMLIERARVPFDRNEDGSFNLTREGGHSTPRILHCRDYSGRAIMEGLTAQI
MVHPRITRLHHRAAIDLLTSRHHARTSQYRYEINNRCMGAYVLNEESGEPETILADWTVLATGGVGQVFLHSTNAPGCVG
TGVSMAFRAGVDLANLEFMQFHPTALYEERSNRRSLITEAMRGEGARLLNHKGQAFMQGYDPRGDLAPRDVVAQAMMDEM
LHNGAPCLFLDVSGVEQDLPTRFPTVFQKCLEAGIDIRREPIPVVPAAHYFCGGVLTDERGRTSMRGLYAIGECACTGLH
GANRLASTSLLEALVWGVSCGKDLNRRLKAESGLPKGLAAAIPDWLHEGDERRDDPALVAQDWTNIRNTMWNYVGISRTE
ARLRRAFEDMRDLVRHIHDFYKYTRISRRLVDLFHGSQTAYVIIQAAMRNRASLGCHHRVD

Specific function: Catalyzes the oxidation of L-aspartate to iminoaspartate [H]

COG id: COG0029

COG function: function code H; Aspartate oxidase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the FAD-dependent oxidoreductase 2 family. NadB subfamily [H]

Homologues:

Organism=Homo sapiens, GI156416003, Length=482, Percent_Identity=29.4605809128631, Blast_Score=175, Evalue=9e-44,
Organism=Escherichia coli, GI1788928, Length=537, Percent_Identity=41.8994413407821, Blast_Score=374, Evalue=1e-105,
Organism=Escherichia coli, GI1790597, Length=531, Percent_Identity=30.1318267419962, Blast_Score=192, Evalue=6e-50,
Organism=Escherichia coli, GI1786942, Length=401, Percent_Identity=33.6658354114713, Blast_Score=164, Evalue=1e-41,
Organism=Caenorhabditis elegans, GI17550100, Length=425, Percent_Identity=30.3529411764706, Blast_Score=173, Evalue=2e-43,
Organism=Caenorhabditis elegans, GI17505833, Length=369, Percent_Identity=31.1653116531165, Blast_Score=169, Evalue=3e-42,
Organism=Caenorhabditis elegans, GI71986328, Length=486, Percent_Identity=28.1893004115226, Blast_Score=103, Evalue=2e-22,
Organism=Saccharomyces cerevisiae, GI6322416, Length=558, Percent_Identity=29.5698924731183, Blast_Score=176, Evalue=8e-45,
Organism=Saccharomyces cerevisiae, GI6322701, Length=561, Percent_Identity=28.8770053475936, Blast_Score=172, Evalue=9e-44,
Organism=Drosophila melanogaster, GI24663005, Length=452, Percent_Identity=30.7522123893805, Blast_Score=176, Evalue=4e-44,
Organism=Drosophila melanogaster, GI17137288, Length=516, Percent_Identity=29.4573643410853, Blast_Score=175, Evalue=7e-44,
Organism=Drosophila melanogaster, GI24655642, Length=516, Percent_Identity=29.4573643410853, Blast_Score=175, Evalue=7e-44,
Organism=Drosophila melanogaster, GI24655647, Length=516, Percent_Identity=29.4573643410853, Blast_Score=175, Evalue=7e-44,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003953
- InterPro:   IPR013027
- InterPro:   IPR015939
- InterPro:   IPR004112
- InterPro:   IPR005288 [H]

Pfam domain/function: PF00890 FAD_binding_2; PF02910 Succ_DH_flav_C [H]

EC number: =1.4.3.16 [H]

Molecular weight: Translated: 59675; Mature: 59675

Theoretical pI: Translated: 7.46; Mature: 7.46

Prosite motif: PS00013 PROKAR_LIPOPROTEIN

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.4 %Cys     (Translated Protein)
3.0 %Met     (Translated Protein)
5.4 %Cys+Met (Translated Protein)
2.4 %Cys     (Mature Protein)
3.0 %Met     (Mature Protein)
5.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNVSRRHVPVLIIGSGIAGCTAALTLADAGHEVLLINAGSQLLDGNSELAQGGIIYRADP
CCCCCCCCCEEEEECCCCHHHHHHHEECCCCEEEEEECCCHHHCCCCHHHCCCEEEECCC
ARNGGPGTAGGQNSDDAAALEKDILVAGHNYNYRKAVRFLCRQGPSCVDDMLIERARVPF
CCCCCCCCCCCCCCCHHHHHHCCEEEECCCCCHHHHHHHHHHCCCCHHHHHHHHHHCCCC
DRNEDGSFNLTREGGHSTPRILHCRDYSGRAIMEGLTAQIMVHPRITRLHHRAAIDLLTS
CCCCCCCEEEEECCCCCCCEEEEECCCCCHHHHHCCCEEEEECCHHHHHHHHHHHHHHHH
RHHARTSQYRYEINNRCMGAYVLNEESGEPETILADWTVLATGGVGQVFLHSTNAPGCVG
HHHHHHHHHHEEECCCEEEEEEEECCCCCCCEEEEEEEEEEECCCCEEEEECCCCCCCCC
TGVSMAFRAGVDLANLEFMQFHPTALYEERSNRRSLITEAMRGEGARLLNHKGQAFMQGY
CCHHHHHHCCCCCCCEEEEEECCHHHHHHHCCHHHHHHHHHHCCCCHHHHCCCCHHHCCC
DPRGDLAPRDVVAQAMMDEMLHNGAPCLFLDVSGVEQDLPTRFPTVFQKCLEAGIDIRRE
CCCCCCCHHHHHHHHHHHHHHHCCCCEEEEECCCCCHHCCCHHHHHHHHHHHCCCCCCCC
PIPVVPAAHYFCGGVLTDERGRTSMRGLYAIGECACTGLHGANRLASTSLLEALVWGVSC
CCCCCCCHHHHHCCCEECCCCCHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHC
GKDLNRRLKAESGLPKGLAAAIPDWLHEGDERRDDPALVAQDWTNIRNTMWNYVGISRTE
CHHHHHHHHHHCCCCCHHHHHCCHHHHCCCCCCCCCCEEHHHHHHHHHHHHHHCCCCHHH
ARLRRAFEDMRDLVRHIHDFYKYTRISRRLVDLFHGSQTAYVIIQAAMRNRASLGCHHRV
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEEEEHHHHCHHHCCCCCCC
D
C
>Mature Secondary Structure
MNVSRRHVPVLIIGSGIAGCTAALTLADAGHEVLLINAGSQLLDGNSELAQGGIIYRADP
CCCCCCCCCEEEEECCCCHHHHHHHEECCCCEEEEEECCCHHHCCCCHHHCCCEEEECCC
ARNGGPGTAGGQNSDDAAALEKDILVAGHNYNYRKAVRFLCRQGPSCVDDMLIERARVPF
CCCCCCCCCCCCCCCHHHHHHCCEEEECCCCCHHHHHHHHHHCCCCHHHHHHHHHHCCCC
DRNEDGSFNLTREGGHSTPRILHCRDYSGRAIMEGLTAQIMVHPRITRLHHRAAIDLLTS
CCCCCCCEEEEECCCCCCCEEEEECCCCCHHHHHCCCEEEEECCHHHHHHHHHHHHHHHH
RHHARTSQYRYEINNRCMGAYVLNEESGEPETILADWTVLATGGVGQVFLHSTNAPGCVG
HHHHHHHHHHEEECCCEEEEEEEECCCCCCCEEEEEEEEEEECCCCEEEEECCCCCCCCC
TGVSMAFRAGVDLANLEFMQFHPTALYEERSNRRSLITEAMRGEGARLLNHKGQAFMQGY
CCHHHHHHCCCCCCCEEEEEECCHHHHHHHCCHHHHHHHHHHCCCCHHHHCCCCHHHCCC
DPRGDLAPRDVVAQAMMDEMLHNGAPCLFLDVSGVEQDLPTRFPTVFQKCLEAGIDIRRE
CCCCCCCHHHHHHHHHHHHHHHCCCCEEEEECCCCCHHCCCHHHHHHHHHHHCCCCCCCC
PIPVVPAAHYFCGGVLTDERGRTSMRGLYAIGECACTGLHGANRLASTSLLEALVWGVSC
CCCCCCCHHHHHCCCEECCCCCHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHC
GKDLNRRLKAESGLPKGLAAAIPDWLHEGDERRDDPALVAQDWTNIRNTMWNYVGISRTE
CHHHHHHHHHHCCCCCHHHHHCCHHHHCCCCCCCCCCEEHHHHHHHHHHHHHHCCCCHHH
ARLRRAFEDMRDLVRHIHDFYKYTRISRRLVDLFHGSQTAYVIIQAAMRNRASLGCHHRV
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEEEEHHHHCHHHCCCCCCC
D
C

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 7737518; 10984043; 7961421 [H]