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The map label for this gene is rpoA

Identifier: 220903960

GI number: 220903960

Start: 814206

End: 815249

Strand: Direct

Name: rpoA

Synonym: Ddes_0686

Alternate gene names: 220903960

Gene position: 814206-815249 (Clockwise)

Preceding gene: 220903959

Following gene: 220903961

Centisome position: 28.34

GC content: 56.13

Gene sequence:

>1044_bases
ATGCTTATCAAACAGGGCGAACGCCTTATCAATGCGCGCAATTGGTCCGAGCTTGTCAAGCCCGACCAGATTTTGCGCGA
AGAAGAGACGGCCAGCGGCACGCACGGCAAGTTTGTCTGCGAGCCTTTGGAGCGGGGCTACGGCACCACCATCGGCAATG
CCATGCGGCGCGTCCTTCTGGCCTCCCTTCAGGGGGCGGCCTTTGTGTCGGTGAAAATCATCGGCGTACAGCACGAGTTC
ACCACCATCCATGGCGTGCTGGAAGACATCACTGATGTCATCCTGAACATCAAGCAGGTTCGCCTGCGCATGGATACGGA
AGAACCCCAGCGTCTGACCCTGCGCGTTGAGCGCAAGGGACCGGTGACGGCGGCCGACATTGTGGCCAATCAGCACGTTG
AAGTGCTTAACCCTGATCTGCACATCGCCACTCTTACCGAAGACGTGGTGCTGGAGATGGAGTTGGAGGTCCGCATGGGC
AAGGGCTATGTGCCCGCCGACATGCACGAGGGCCTGGCCGATGAAATCGGCCTCATCAAGCTGGATTCCAGCTTTTCCCC
GGTGCGCAAGGTCGCCTATACGGTGGAACAGGCCCGCGTGGGCCAGATGACCAACTATGACCGTCTTCTGCTTGAAGTGT
GGACCGACGGCTCGCTGACGCCTGAAGATGCCATTGCTTACAGCGCCAAGATCATCAAGGATCAGATATCCGTATTCATC
AACTTTGATGAACGTGTATCCGGCGACATGCGCAACGGCAGCGGCGAAAGCGGAGAAGTGAACGAACATCTGTTCAAAAG
CATTGACGATCTTGAGCTTTCCGTTCGTGCCACCAACTGCCTGCGCAGCGCCAATATCGGCCTTGTGGGTGAACTGGTGC
AGCGTACTGAAGCCGAGATGCTCAAGACCAAGAATTTCGGCCGCAAATCGCTGGACGAAATTAAGGGTGTGCTTTTGGGC
ATGGGGCTTGACTTCGGCATGAAGGTCGACTCCTTCGACAAAAAATATCAGGAATGGAAAAGGAAGCAGCAAAATGAGGC
ATAG

Upstream 100 bases:

>100_bases
CAAGGGTACTGTCAAGGCAATGCCGCAGCGTGACGACATCCAGTTCCCCGTCAACGAGCAGCTTATCGTCGAACTTTACT
CGAAATAACCGGGGGTTCGC

Downstream 100 bases:

>100_bases
CAATTCCGGCAGGAAATTCTCGCGTACCCCTGCGCACCGTAAGGCCATGTTGCACAATCTCGCCAAGGCGCTGCTGATCC
ACGGCAAAATCCGCACCACG

Product: DNA-directed RNA polymerase subunit alpha

Products: NA

Alternate protein names: RNAP subunit alpha; RNA polymerase subunit alpha; Transcriptase subunit alpha

Number of amino acids: Translated: 347; Mature: 347

Protein sequence:

>347_residues
MLIKQGERLINARNWSELVKPDQILREEETASGTHGKFVCEPLERGYGTTIGNAMRRVLLASLQGAAFVSVKIIGVQHEF
TTIHGVLEDITDVILNIKQVRLRMDTEEPQRLTLRVERKGPVTAADIVANQHVEVLNPDLHIATLTEDVVLEMELEVRMG
KGYVPADMHEGLADEIGLIKLDSSFSPVRKVAYTVEQARVGQMTNYDRLLLEVWTDGSLTPEDAIAYSAKIIKDQISVFI
NFDERVSGDMRNGSGESGEVNEHLFKSIDDLELSVRATNCLRSANIGLVGELVQRTEAEMLKTKNFGRKSLDEIKGVLLG
MGLDFGMKVDSFDKKYQEWKRKQQNEA

Sequences:

>Translated_347_residues
MLIKQGERLINARNWSELVKPDQILREEETASGTHGKFVCEPLERGYGTTIGNAMRRVLLASLQGAAFVSVKIIGVQHEF
TTIHGVLEDITDVILNIKQVRLRMDTEEPQRLTLRVERKGPVTAADIVANQHVEVLNPDLHIATLTEDVVLEMELEVRMG
KGYVPADMHEGLADEIGLIKLDSSFSPVRKVAYTVEQARVGQMTNYDRLLLEVWTDGSLTPEDAIAYSAKIIKDQISVFI
NFDERVSGDMRNGSGESGEVNEHLFKSIDDLELSVRATNCLRSANIGLVGELVQRTEAEMLKTKNFGRKSLDEIKGVLLG
MGLDFGMKVDSFDKKYQEWKRKQQNEA
>Mature_347_residues
MLIKQGERLINARNWSELVKPDQILREEETASGTHGKFVCEPLERGYGTTIGNAMRRVLLASLQGAAFVSVKIIGVQHEF
TTIHGVLEDITDVILNIKQVRLRMDTEEPQRLTLRVERKGPVTAADIVANQHVEVLNPDLHIATLTEDVVLEMELEVRMG
KGYVPADMHEGLADEIGLIKLDSSFSPVRKVAYTVEQARVGQMTNYDRLLLEVWTDGSLTPEDAIAYSAKIIKDQISVFI
NFDERVSGDMRNGSGESGEVNEHLFKSIDDLELSVRATNCLRSANIGLVGELVQRTEAEMLKTKNFGRKSLDEIKGVLLG
MGLDFGMKVDSFDKKYQEWKRKQQNEA

Specific function: DNA-dependent RNA polymerase catalyzes the transcription of DNA into RNA using the four ribonucleoside triphosphates as substrates

COG id: COG0202

COG function: function code K; DNA-directed RNA polymerase, alpha subunit/40 kD subunit

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the RNA polymerase alpha chain family

Homologues:

Organism=Escherichia coli, GI1789690, Length=321, Percent_Identity=42.9906542056075, Blast_Score=252, Evalue=3e-68,

Paralogues:

None

Copy number: 850 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 560 Molecules/Cell In: Stationary-Phase, Rich-Media (Based on E. coli). 6847 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. 7,000 Molecules/Cell In: Glucose minimal media

Swissprot (AC and ID): RPOA_DESDA (B8IYL7)

Other databases:

- EMBL:   CP001358
- RefSeq:   YP_002479272.1
- GeneID:   7284359
- GenomeReviews:   CP001358_GR
- KEGG:   dds:Ddes_0686
- HOGENOM:   HBG430844
- ProtClustDB:   PRK05182
- HAMAP:   MF_00059
- InterPro:   IPR011261
- InterPro:   IPR011262
- InterPro:   IPR009025
- InterPro:   IPR011263
- InterPro:   IPR011260
- InterPro:   IPR011773
- Gene3D:   G3DSA:2.170.120.12
- ProDom:   PD001179
- SMART:   SM00662
- TIGRFAMs:   TIGR02027

Pfam domain/function: PF01000 RNA_pol_A_bac; PF03118 RNA_pol_A_CTD; PF01193 RNA_pol_L; SSF47789 RNAP_alpha_C; SSF56553 RNAP_insert; SSF55257 RNAP_RBP11-like

EC number: =2.7.7.6

Molecular weight: Translated: 38884; Mature: 38884

Theoretical pI: Translated: 5.01; Mature: 5.01

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
3.2 %Met     (Translated Protein)
3.7 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
3.2 %Met     (Mature Protein)
3.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLIKQGERLINARNWSELVKPDQILREEETASGTHGKFVCEPLERGYGTTIGNAMRRVLL
CCCCCCHHHHCCCCHHHHCCHHHHHHHHHCCCCCCCCEEECHHHCCCCCHHHHHHHHHHH
ASLQGAAFVSVKIIGVQHEFTTIHGVLEDITDVILNIKQVRLRMDTEEPQRLTLRVERKG
HHCCCCEEEEEEEEEECHHHHHHHHHHHHHHHHHHHHHHHHEECCCCCCCEEEEEEECCC
PVTAADIVANQHVEVLNPDLHIATLTEDVVLEMELEVRMGKGYVPADMHEGLADEIGLIK
CCCHHHHHHCCCCEEECCCEEEEEECCCEEEEEEEEEEECCCCCCCHHHCCHHHCCCEEE
LDSSFSPVRKVAYTVEQARVGQMTNYDRLLLEVWTDGSLTPEDAIAYSAKIIKDQISVFI
ECCCCCHHHHHHHHHHHHHHCCCCCHHHEEEEEECCCCCCHHHHHHHHHHHHHHEEEEEE
NFDERVSGDMRNGSGESGEVNEHLFKSIDDLELSVRATNCLRSANIGLVGELVQRTEAEM
EEHHHHCCCCCCCCCCCCCHHHHHHHCCCHHEEEEHHHHHHHHCCCCHHHHHHHHHHHHH
LKTKNFGRKSLDEIKGVLLGMGLDFGMKVDSFDKKYQEWKRKQQNEA
HHHHCCCHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHCCCC
>Mature Secondary Structure
MLIKQGERLINARNWSELVKPDQILREEETASGTHGKFVCEPLERGYGTTIGNAMRRVLL
CCCCCCHHHHCCCCHHHHCCHHHHHHHHHCCCCCCCCEEECHHHCCCCCHHHHHHHHHHH
ASLQGAAFVSVKIIGVQHEFTTIHGVLEDITDVILNIKQVRLRMDTEEPQRLTLRVERKG
HHCCCCEEEEEEEEEECHHHHHHHHHHHHHHHHHHHHHHHHEECCCCCCCEEEEEEECCC
PVTAADIVANQHVEVLNPDLHIATLTEDVVLEMELEVRMGKGYVPADMHEGLADEIGLIK
CCCHHHHHHCCCCEEECCCEEEEEECCCEEEEEEEEEEECCCCCCCHHHCCHHHCCCEEE
LDSSFSPVRKVAYTVEQARVGQMTNYDRLLLEVWTDGSLTPEDAIAYSAKIIKDQISVFI
ECCCCCHHHHHHHHHHHHHHCCCCCHHHEEEEEECCCCCCHHHHHHHHHHHHHHEEEEEE
NFDERVSGDMRNGSGESGEVNEHLFKSIDDLELSVRATNCLRSANIGLVGELVQRTEAEM
EEHHHHCCCCCCCCCCCCCHHHHHHHCCCHHEEEEHHHHHHHHCCCCHHHHHHHHHHHHH
LKTKNFGRKSLDEIKGVLLGMGLDFGMKVDSFDKKYQEWKRKQQNEA
HHHHCCCHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA