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The map label for this gene is thiC [H]

Identifier: 220903903

GI number: 220903903

Start: 756204

End: 757484

Strand: Reverse

Name: thiC [H]

Synonym: Ddes_0628

Alternate gene names: 220903903

Gene position: 757484-756204 (Counterclockwise)

Preceding gene: 220903906

Following gene: 220903902

Centisome position: 26.36

GC content: 63.54

Gene sequence:

>1281_bases
ATGTCTACCACACTTTTTTCGCAGAATACCGCATTGCGCGGTCTTCTGGACTCCCATCTGGACAGCCTGTCCGCTGAAGA
GCAGCTCGACGCCCAAAGCGTGACCGCCGCCCTTGAGGCCGGAACTATGGTGCTGCTGGGCAACCCCGCCCATAAGGGAC
TCAAACCCATTCTTGTGGGTCAGCCCGCCAGGGTCAAGGTGAACGCCAATATCGGCACGTCTCCCCTGAACAACTGCCCC
CGCACCGAGGAGCGCAAGATACAGGCCGCCCTTGAAGCCGGAGCCGATACGGTTATGGATCTTTCCATCGCCGGCGATCT
GGACGCCCTGCGCCTCGGCATGCTGGAGGCCTGCCCCCGGCCGCTGGGTACGGTTCCCCTGTACGCCGTGGGCCAGCGCA
TACTGGACGCGGAGCGCGACATTGCCAGCATGAACCCGGACGAGCTTTTTGACGAAATCGCCAAACAGGCCCGGCAAGGG
GTGGATTTTGTCACCGTTCACTGCGGGCTTTCGCGGCGCGGAGCCGAAATGGCCGTCAAAAACAACCGCGCTCTGGGCAT
TGTCTCGCGCGGCGGCTCCATGCTGGCCCGCTGGATGCTCGAAAACGACCGCGAAAACCCCTTGCTGGAGCATTTTGACC
GCCTGCTCGACATCTGCCTTCCCTACAACGTGACCCTCTCCCTCGGCGACGGACTGCGCCCCGGCGCGGGCGTTGATGCG
GGCGATGCCGCCCAGTGGGAAGAGGTCATCAATCTGGGGCGCCTTGCCAGATACGCGCTTGAACGCGGCGTGCAATGCAT
GATTGAAGGCCCCGGCCACGTGCCCCTCAACCAGGTGCGCACCCAGATTCAGGGCATAAAGCGCCTCACCCATAATGCCC
CGCTCTATGTTCTCGGCCCCCTGTGCTGCGACAGCGCGCCGGGCTACGACCATATCGCCGGAGCCATCGGCGGCGCACTA
GGCGTGGAAGCAGGCGTGGACTTTCTCTGCTACCTCACCCCTGCCGAGCACCTCACCCTGCCTGACGAGGCAGACGTGCG
CGCCGGGGTCATGGCTTCGCGCGTGGCCGGGCATGTAGGCGAAGTGGCTTTGGGCCACCCCCGCGCCGTGGCACGTGAGG
CAGCCATGAACGCCGCTCGCAAGGCGCTGGACTGGGAAGGCATGACCAAAGCCGCCCTGGATCCACAAATGCTTGAAAAA
CGGCGTGAGGAGCACAAGACTGAAGAAGTCTGCGCCATGTGCGGCAAGTTCTGCGCGGTCAAGATGCTTCAGGATCACTA
G

Upstream 100 bases:

>100_bases
AACCTGACGCAGTTCGCACTGCCGGAGGGAAGCCGCGGCCACAAGCTGTTCCGCTGCTTGTGGTTTTTTTGCGCCCTGCG
TCGCAACCCGCGAGGCACAC

Downstream 100 bases:

>100_bases
GGCATTTGCGCCGTAACGTGCTTACGGAGCGAGGAAACAGCCCTGCGGCCCTCGCTGGTGGCTTGCTTCGGCGTGACGGG
CGAAACAGGTTCGCCTTGCA

Product: thiamine biosynthesis protein ThiC

Products: @AMIN06.txt*4-amino-2-methyl-5-hydroxymethylpyrimidine!; 4-amino-5-hydroxymethyl-2-methylpyrimidine-phosphate [C]

Alternate protein names: Hydroxymethylpyrimidine phosphate synthase; HMP-P synthase; HMP-phosphate synthase; HMPP synthase; Thiamine biosynthesis protein thiC [H]

Number of amino acids: Translated: 426; Mature: 425

Protein sequence:

>426_residues
MSTTLFSQNTALRGLLDSHLDSLSAEEQLDAQSVTAALEAGTMVLLGNPAHKGLKPILVGQPARVKVNANIGTSPLNNCP
RTEERKIQAALEAGADTVMDLSIAGDLDALRLGMLEACPRPLGTVPLYAVGQRILDAERDIASMNPDELFDEIAKQARQG
VDFVTVHCGLSRRGAEMAVKNNRALGIVSRGGSMLARWMLENDRENPLLEHFDRLLDICLPYNVTLSLGDGLRPGAGVDA
GDAAQWEEVINLGRLARYALERGVQCMIEGPGHVPLNQVRTQIQGIKRLTHNAPLYVLGPLCCDSAPGYDHIAGAIGGAL
GVEAGVDFLCYLTPAEHLTLPDEADVRAGVMASRVAGHVGEVALGHPRAVAREAAMNAARKALDWEGMTKAALDPQMLEK
RREEHKTEEVCAMCGKFCAVKMLQDH

Sequences:

>Translated_426_residues
MSTTLFSQNTALRGLLDSHLDSLSAEEQLDAQSVTAALEAGTMVLLGNPAHKGLKPILVGQPARVKVNANIGTSPLNNCP
RTEERKIQAALEAGADTVMDLSIAGDLDALRLGMLEACPRPLGTVPLYAVGQRILDAERDIASMNPDELFDEIAKQARQG
VDFVTVHCGLSRRGAEMAVKNNRALGIVSRGGSMLARWMLENDRENPLLEHFDRLLDICLPYNVTLSLGDGLRPGAGVDA
GDAAQWEEVINLGRLARYALERGVQCMIEGPGHVPLNQVRTQIQGIKRLTHNAPLYVLGPLCCDSAPGYDHIAGAIGGAL
GVEAGVDFLCYLTPAEHLTLPDEADVRAGVMASRVAGHVGEVALGHPRAVAREAAMNAARKALDWEGMTKAALDPQMLEK
RREEHKTEEVCAMCGKFCAVKMLQDH
>Mature_425_residues
STTLFSQNTALRGLLDSHLDSLSAEEQLDAQSVTAALEAGTMVLLGNPAHKGLKPILVGQPARVKVNANIGTSPLNNCPR
TEERKIQAALEAGADTVMDLSIAGDLDALRLGMLEACPRPLGTVPLYAVGQRILDAERDIASMNPDELFDEIAKQARQGV
DFVTVHCGLSRRGAEMAVKNNRALGIVSRGGSMLARWMLENDRENPLLEHFDRLLDICLPYNVTLSLGDGLRPGAGVDAG
DAAQWEEVINLGRLARYALERGVQCMIEGPGHVPLNQVRTQIQGIKRLTHNAPLYVLGPLCCDSAPGYDHIAGAIGGALG
VEAGVDFLCYLTPAEHLTLPDEADVRAGVMASRVAGHVGEVALGHPRAVAREAAMNAARKALDWEGMTKAALDPQMLEKR
REEHKTEEVCAMCGKFCAVKMLQDH

Specific function: Catalyzes the synthesis of the hydroxymethylpyrimidine phosphate (HMP-P) moiety of thiamine from aminoimidazole ribotide (AIR) in a radical S-adenosyl-L-methionine (SAM)-dependent reaction [H]

COG id: COG0422

COG function: function code H; Thiamine biosynthesis protein ThiC

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the thiC family [H]

Homologues:

Organism=Escherichia coli, GI1790427, Length=406, Percent_Identity=41.1330049261084, Blast_Score=311, Evalue=5e-86,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002817 [H]

Pfam domain/function: PF01964 ThiC [H]

EC number: NA

Molecular weight: Translated: 45721; Mature: 45590

Theoretical pI: Translated: 5.45; Mature: 5.45

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.6 %Cys     (Translated Protein)
3.5 %Met     (Translated Protein)
6.1 %Cys+Met (Translated Protein)
2.6 %Cys     (Mature Protein)
3.3 %Met     (Mature Protein)
5.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSTTLFSQNTALRGLLDSHLDSLSAEEQLDAQSVTAALEAGTMVLLGNPAHKGLKPILVG
CCCCCCCCCHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHCCCEEEECCCCCCCCCEEEEC
QPARVKVNANIGTSPLNNCPRTEERKIQAALEAGADTVMDLSIAGDLDALRLGMLEACPR
CCCEEEEECCCCCCCCCCCCCCHHHHHHHHHHCCCCCEEEEEECCCHHHHHHHHHHHCCC
PLGTVPLYAVGQRILDAERDIASMNPDELFDEIAKQARQGVDFVTVHCGLSRRGAEMAVK
CCCCCCHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHCCCCEEEEECCCCCCCCHHEEC
NNRALGIVSRGGSMLARWMLENDRENPLLEHFDRLLDICLPYNVTLSLGDGLRPGAGVDA
CCCEEEEEECCHHHHHHHHHHCCCCCHHHHHHHHHHHHHCCCCEEEECCCCCCCCCCCCC
GDAAQWEEVINLGRLARYALERGVQCMIEGPGHVPLNQVRTQIQGIKRLTHNAPLYVLGP
CCHHHHHHHHHHHHHHHHHHHCCCEEEEECCCCCCHHHHHHHHHHHHHHHCCCCEEEECC
LCCDSAPGYDHIAGAIGGALGVEAGVDFLCYLTPAEHLTLPDEADVRAGVMASRVAGHVG
HHCCCCCCHHHHHHHHHHHHHHHHCCCEEEEECCHHHCCCCCCCCHHHHHHHHHHHHHHC
EVALGHPRAVAREAAMNAARKALDWEGMTKAALDPQMLEKRREEHKTEEVCAMCGKFCAV
CCCCCCCHHHHHHHHHHHHHHHHCCCCCHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHH
KMLQDH
HHHHCC
>Mature Secondary Structure 
STTLFSQNTALRGLLDSHLDSLSAEEQLDAQSVTAALEAGTMVLLGNPAHKGLKPILVG
CCCCCCCCHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHCCCEEEECCCCCCCCCEEEEC
QPARVKVNANIGTSPLNNCPRTEERKIQAALEAGADTVMDLSIAGDLDALRLGMLEACPR
CCCEEEEECCCCCCCCCCCCCCHHHHHHHHHHCCCCCEEEEEECCCHHHHHHHHHHHCCC
PLGTVPLYAVGQRILDAERDIASMNPDELFDEIAKQARQGVDFVTVHCGLSRRGAEMAVK
CCCCCCHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHCCCCEEEEECCCCCCCCHHEEC
NNRALGIVSRGGSMLARWMLENDRENPLLEHFDRLLDICLPYNVTLSLGDGLRPGAGVDA
CCCEEEEEECCHHHHHHHHHHCCCCCHHHHHHHHHHHHHCCCCEEEECCCCCCCCCCCCC
GDAAQWEEVINLGRLARYALERGVQCMIEGPGHVPLNQVRTQIQGIKRLTHNAPLYVLGP
CCHHHHHHHHHHHHHHHHHHHCCCEEEEECCCCCCHHHHHHHHHHHHHHHCCCCEEEECC
LCCDSAPGYDHIAGAIGGALGVEAGVDFLCYLTPAEHLTLPDEADVRAGVMASRVAGHVG
HHCCCCCCHHHHHHHHHHHHHHHHCCCEEEEECCHHHCCCCCCCCHHHHHHHHHHHHHHC
EVALGHPRAVAREAAMNAARKALDWEGMTKAALDPQMLEKRREEHKTEEVCAMCGKFCAV
CCCCCCCHHHHHHHHHHHHHHHHCCCCCHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHH
KMLQDH
HHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: ?; ?; 5'-phosphoribosyl-5-aminoimidazole [C]

Specific reaction: Synthesis of @AMIN06.txt*4-amino-2-methyl-5-hydroxymethylpyrimidine! 5'-phosphoribosyl-5-aminoimidazole = 4-amino-5-hydroxymethyl-2-methylpyrimidine-phosphate [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA