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The map label for this gene is trmH [H]

Identifier: 220903872

GI number: 220903872

Start: 723031

End: 723783

Strand: Direct

Name: trmH [H]

Synonym: Ddes_0597

Alternate gene names: 220903872

Gene position: 723031-723783 (Clockwise)

Preceding gene: 220903871

Following gene: 220903874

Centisome position: 25.16

GC content: 58.96

Gene sequence:

>753_bases
GTGGACGGACATGCTGAAGTCCAAGGCATTCTGAGGGGTGCGCGCCCGCCCCTGCTCAAGCCCCCGGAACCTGTTGCCGG
GGGCTTTTTGCGATGTGGCTTGCCTGCGCGGGTTTTTTTTGAGAAGTATCAGGACCTGAACAGAACAGCAAAGGAAGACC
TCATGACCAAGGAACCCACGGAACGGCGCAAGGCCCGTCTGCTTGAAGTGCTGGCCCACCGCCAGCCGGACCTCACCCTG
GTGCTGGCAAATATTCACGATCCGCATAATGTTTCAGCTATTTATCGTTCGTGCGATGCCTTTGGCGTCAGCCGCGTCAA
CCTGTATTATACCAATACGCCTTTTCCCGCGCTTGGGCGCAAGACATCGGCCTCCGCCAGAAAATGGGTGGAGAGCGCGC
GCCACAAAGACAGCGCAGCGCTTATGGAAAGCCTGCGCGGGCAAAACATGCAGGTGCTGGCCACATCCTGCTCGCCCACG
GCCCGTCCGCTGCGCGACTGGGATTTTACCCGTCCCACAGCCGTCATTATGGGCAACGAGCACAGCGGTGTAGAACCGGA
ACTGTTGCAGATGGCCGATGGCGAGCTTTATATCCCCATGTACGGGATGATCCAGAGTTTTAACGTATCTGTGGCGGCGG
CCATTATTTTGTCCGAGGCTGCCCGTCAGCGGGAGGCCGCAGGCATGTACGCCACGCCGCGCATGGCTGCTGCGGCTCTG
GAGGAAAAACTGCAGGAATGGCTGGAGAAATAG

Upstream 100 bases:

>100_bases
AGGATCCCGCAGGCCTCATAGCCCGCGCCCTTGCCTGGGACATGGGGCGTCAGGTACTGGGATGGGTGGACCCGAGCCGT
CCGGCAGGCAGGGAATCTTC

Downstream 100 bases:

>100_bases
CCCGTTGCAGTCACGGAGTTTTTACCGCGCCGCTTTCCCGATGATATGTGCCGGAGAAAGCGGCGCGGCCGTTTGTGGCG
GCACGCCTGCCTGCGGGTAC

Product: tRNA guanosine-2'-O-methyltransferase

Products: NA

Alternate protein names: tRNA [Gm18] methyltransferase [H]

Number of amino acids: Translated: 250; Mature: 250

Protein sequence:

>250_residues
MDGHAEVQGILRGARPPLLKPPEPVAGGFLRCGLPARVFFEKYQDLNRTAKEDLMTKEPTERRKARLLEVLAHRQPDLTL
VLANIHDPHNVSAIYRSCDAFGVSRVNLYYTNTPFPALGRKTSASARKWVESARHKDSAALMESLRGQNMQVLATSCSPT
ARPLRDWDFTRPTAVIMGNEHSGVEPELLQMADGELYIPMYGMIQSFNVSVAAAIILSEAARQREAAGMYATPRMAAAAL
EEKLQEWLEK

Sequences:

>Translated_250_residues
MDGHAEVQGILRGARPPLLKPPEPVAGGFLRCGLPARVFFEKYQDLNRTAKEDLMTKEPTERRKARLLEVLAHRQPDLTL
VLANIHDPHNVSAIYRSCDAFGVSRVNLYYTNTPFPALGRKTSASARKWVESARHKDSAALMESLRGQNMQVLATSCSPT
ARPLRDWDFTRPTAVIMGNEHSGVEPELLQMADGELYIPMYGMIQSFNVSVAAAIILSEAARQREAAGMYATPRMAAAAL
EEKLQEWLEK
>Mature_250_residues
MDGHAEVQGILRGARPPLLKPPEPVAGGFLRCGLPARVFFEKYQDLNRTAKEDLMTKEPTERRKARLLEVLAHRQPDLTL
VLANIHDPHNVSAIYRSCDAFGVSRVNLYYTNTPFPALGRKTSASARKWVESARHKDSAALMESLRGQNMQVLATSCSPT
ARPLRDWDFTRPTAVIMGNEHSGVEPELLQMADGELYIPMYGMIQSFNVSVAAAIILSEAARQREAAGMYATPRMAAAAL
EEKLQEWLEK

Specific function: Specifically methylates guanosine-18 in various tRNAs [H]

COG id: COG0566

COG function: function code J; rRNA methylases

Gene ontology:

Cell location: Cytoplasm (Potential) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the RNA methyltransferase TrmH family [H]

Homologues:

Organism=Escherichia coli, GI1790083, Length=168, Percent_Identity=46.4285714285714, Blast_Score=160, Evalue=9e-41,
Organism=Escherichia coli, GI1790623, Length=151, Percent_Identity=34.4370860927152, Blast_Score=69, Evalue=3e-13,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR022724
- InterPro:   IPR001537 [H]

Pfam domain/function: PF12105 SpoU_methylas_C; PF00588 SpoU_methylase [H]

EC number: =2.1.1.34 [H]

Molecular weight: Translated: 27817; Mature: 27817

Theoretical pI: Translated: 8.46; Mature: 8.46

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.2 %Cys     (Translated Protein)
4.0 %Met     (Translated Protein)
5.2 %Cys+Met (Translated Protein)
1.2 %Cys     (Mature Protein)
4.0 %Met     (Mature Protein)
5.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MDGHAEVQGILRGARPPLLKPPEPVAGGFLRCGLPARVFFEKYQDLNRTAKEDLMTKEPT
CCCHHHHHHHHHCCCCCCCCCCCCCCCCHHHCCCHHHHHHHHHHHHHHHHHHHHHCCCHH
ERRKARLLEVLAHRQPDLTLVLANIHDPHNVSAIYRSCDAFGVSRVNLYYTNTPFPALGR
HHHHHHHHHHHHCCCCCEEEEEECCCCCHHHHHHHHHHHHCCCEEEEEEECCCCCCCCCC
KTSASARKWVESARHKDSAALMESLRGQNMQVLATSCSPTARPLRDWDFTRPTAVIMGNE
CCHHHHHHHHHHHHCCHHHHHHHHHCCCCEEEEEECCCCCCCCCCCCCCCCCEEEEECCC
HSGVEPELLQMADGELYIPMYGMIQSFNVSVAAAIILSEAARQREAAGMYATPRMAAAAL
CCCCCHHHHHHCCCCEEEEHHHHHHHCCHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHH
EEKLQEWLEK
HHHHHHHHHC
>Mature Secondary Structure
MDGHAEVQGILRGARPPLLKPPEPVAGGFLRCGLPARVFFEKYQDLNRTAKEDLMTKEPT
CCCHHHHHHHHHCCCCCCCCCCCCCCCCHHHCCCHHHHHHHHHHHHHHHHHHHHHCCCHH
ERRKARLLEVLAHRQPDLTLVLANIHDPHNVSAIYRSCDAFGVSRVNLYYTNTPFPALGR
HHHHHHHHHHHHCCCCCEEEEEECCCCCHHHHHHHHHHHHCCCEEEEEEECCCCCCCCCC
KTSASARKWVESARHKDSAALMESLRGQNMQVLATSCSPTARPLRDWDFTRPTAVIMGNE
CCHHHHHHHHHHHHCCHHHHHHHHHCCCCEEEEEECCCCCCCCCCCCCCCCCEEEEECCC
HSGVEPELLQMADGELYIPMYGMIQSFNVSVAAAIILSEAARQREAAGMYATPRMAAAAL
CCCCCHHHHHHCCCCEEEEHHHHHHHCCHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHH
EEKLQEWLEK
HHHHHHHHHC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]