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The map label for this gene is clpB [H]

Identifier: 220903854

GI number: 220903854

Start: 698104

End: 700710

Strand: Reverse

Name: clpB [H]

Synonym: Ddes_0578

Alternate gene names: 220903854

Gene position: 700710-698104 (Counterclockwise)

Preceding gene: 220903855

Following gene: 220903853

Centisome position: 24.39

GC content: 59.88

Gene sequence:

>2607_bases
ATGGATATCAACAAATTTACGGAAAAAGCACGCGAAGCCGTCAGCGAAGCCCAAAGCCTCGCTGCGGGTATGGGACATCA
GGAAACAGATGTCGAGCATCTGGCGCTGGCCCTGACACAGCAGGAAAACGGCATCGTGCCGCGCATTCTTGAACAGATGG
GCGTACAGCCCCGCGCTCTTTCGGTGGCTCTTGAAGGCGCCGTGCGCAAGCGTCCTTCCGTGTCCGGCGGCGGTATCGAT
CCCACCAAGATCATGATCACCCAGCGCCTGGCCACAGTACTGAACGACGCCCAGAACGAAGCCAAGCGCATGAAGGACGA
ATACGTCAGCGTTGACCACCTGTTTGCCGCACTGACCGAAGTGCCGCCTTCCACGCCCCTTGGCGAAGTGTTTAAAGAGT
ACAAGATTACCCGCGCGTCCTTTGTGGCCGCCATGGAAGACATGCGCGGCGGCGCCCGCGTGACCAGCGCCAACCCCGAA
GACACCTTCGAGGCCCTGACCAAGTACGCGCGCGACCTTGTGGAGGCGGCGCGCCAGGGCAAGATGGATCCGGTCATCGG
TCGTGATGCGGAAATACGCCGCGTCATCCGCATTCTTTCGCGGCGTACCAAGAACAACCCCGTGCTCATCGGCGAGGCGG
GCGTGGGCAAAACAGCCATCGTTGAGGGACTTGCCTTCCGCATCGTCAAGGGCGACGTACCCGAAGGGCTGAAGGGCCGC
AAACTCTTTGCCCTGGACATGGGTGCGCTTATCGCCGGGGCCAAATACCGCGGTGAATTTGAAGAGCGCCTCAAGGCAGT
GCTCAATGAAGTGGAAAAGAGCGAAGGCCAGACGATTCTTTTTATTGATGAACTGCACACCATTGTGGGCGCGGGCAAAA
CCGAAGGCGCAATGGACGCGGGCAACCTGCTCAAACCCATGCTGGCGCGCGGCGAACTGCACTGCATCGGCGCGACCACT
GTGGACGAGTACCGCAAGTATATCGAAAAAGACCCCGCGCTTGAACGCCGCTTTCAGCCCGTCATGGTGGAAGAACCCAC
GGTGGAGGACACCATCTCCATCCTGCGCGGCCTGAAAGAACGTTTTGAGGTGCATCATGGCGTGCGTATCAGCGACTCGG
CCATTGTAGAGGCCGTGATGCTCTCGCACCGCTACATTACTGACCGCCAGCTGCCCGACAAGGCCATTGACCTTATAGAC
GAGGCCGCCGCCCTTATCCGCACCGAGATTGACTCTCTGCCCGCGGATCTTGACGAAGCGAACCGCAAGGTCATGCAGCT
TGAAATCGAGCGTGAGGCCCTGCGGCGCGAAACCGATGCGGCCTCGCGTGAGCGGCTGGAAAAGCTTGAAAATGAACTGG
CCGATCTGCGCATCGAGCAGGCCGACCTGCGCAAGCAGTGGGAAAGTGAAAAAGGCTCCATCAACCAGGTACGCGAAATC
AAGGAGCAGATCGAGCAGACCAAGCTGGCCATCGAGCAGGCCGAACGCGCATACGACCTCAACAAGGCCGCCGAACTGAA
ATACTCAAAGCTGCTCGAGCTTGAAAAAAAACTGGCTGAAGCCGAAAATTCCGACGGTAGCGGCGCTGAAGGCCCCCGCC
TGCTCAAGGAAGAAGTGCGGCCCGACGATGTGGCTGAAATTGTGGGCAAGTGGACAGGCATACCTGTCACCCGCCTGCTG
GAGTCGGAACGAGAAAAGCTGCTGCGCCTGCCCGAACAGTTGCACCAGCGCGTAGTTGGCCAGGACGAAGCCGTTAATGC
GGTATCTGATGCGGTGCTGCGCGCCCGTGCCGGGCTTTCCGACCCGGACAGGCCCACCGGCTCGTTTATTTTCCTCGGTC
CCACGGGCGTGGGTAAAACAGAGCTTTCCAAGGCCCTGGCCGAGGCGCTCTTTGATACTGAAGACAACATGGTCCGCCTG
GACATGAGCGAGTATATGGAAAAGCACTCCGTATCCCGCCTCATCGGCGCGCCTCCCGGATATGTGGGCTATGACGAGGG
CGGCCAGCTTACCGAAGCCGTGCGGCGCAAGCCCTATTCCGTCATCCTTTTTGACGAAATAGAAAAAGCCCACCCGGACG
TGTTCAACACGCTGCTGCAACTGCTGGATGACGGCCGCCTCACTGACAGCCAGGGGCGTACCGTGGACTTCCGCAACTGC
ATTGTCATCATGACGTCCAACATCGGCTCCATGCACCTTCTGGACGGTATTGAAGCGGATGGCGCCCTGAAAGAAGGCGC
ACGCGAACGTGTCATGGAAGAACTGCGGGCGCATTTCAGGCCGGAATTCCTGAACCGCGTGGACGAAACCGTGGTCTTTC
TGCCGCTCAGGCGCGACCAGATCAGCCGCATTGTGGATCTGCAACTGGCCCGCCTGCGCAGGCGCCTTGAAGACCGCAAG
ATCAGGCTCGACATGACCGATGCCGCCAGAAACTTCATCGGCGAGGCGGGTTATGACCCGGTTTACGGCGCACGGCCGCT
CAAGCGCTATGTGCAGCAGGCTGTGGAAACGCCGCTGGCGCGCCAGATTGTGGGCGGCAAGATACGCGACGGCCAGCGTG
TGAACGTGGACGTCAAGGACGAGGCCCTCACCTTTACGGCTGAATAG

Upstream 100 bases:

>100_bases
ACCCGCAGGAAAAGACCCGGGCTGTTATAGCATTTCAAATAATAACGTCTTACGAATATTTTGCCGCCTGCAAGATGCGG
CACTGTCTGGAGGACCCTTT

Downstream 100 bases:

>100_bases
CAAAACGCAGCTCCGGCTGCTGACAAGCATGCAATAAAACATCCCCCGGCGGTTGACCGCCCTGCCTTGCGGCAGGGGCG
GCCAGCCGCCGGGGGATACG

Product: ATP-dependent chaperone ClpB

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 868; Mature: 868

Protein sequence:

>868_residues
MDINKFTEKAREAVSEAQSLAAGMGHQETDVEHLALALTQQENGIVPRILEQMGVQPRALSVALEGAVRKRPSVSGGGID
PTKIMITQRLATVLNDAQNEAKRMKDEYVSVDHLFAALTEVPPSTPLGEVFKEYKITRASFVAAMEDMRGGARVTSANPE
DTFEALTKYARDLVEAARQGKMDPVIGRDAEIRRVIRILSRRTKNNPVLIGEAGVGKTAIVEGLAFRIVKGDVPEGLKGR
KLFALDMGALIAGAKYRGEFEERLKAVLNEVEKSEGQTILFIDELHTIVGAGKTEGAMDAGNLLKPMLARGELHCIGATT
VDEYRKYIEKDPALERRFQPVMVEEPTVEDTISILRGLKERFEVHHGVRISDSAIVEAVMLSHRYITDRQLPDKAIDLID
EAAALIRTEIDSLPADLDEANRKVMQLEIEREALRRETDAASRERLEKLENELADLRIEQADLRKQWESEKGSINQVREI
KEQIEQTKLAIEQAERAYDLNKAAELKYSKLLELEKKLAEAENSDGSGAEGPRLLKEEVRPDDVAEIVGKWTGIPVTRLL
ESEREKLLRLPEQLHQRVVGQDEAVNAVSDAVLRARAGLSDPDRPTGSFIFLGPTGVGKTELSKALAEALFDTEDNMVRL
DMSEYMEKHSVSRLIGAPPGYVGYDEGGQLTEAVRRKPYSVILFDEIEKAHPDVFNTLLQLLDDGRLTDSQGRTVDFRNC
IVIMTSNIGSMHLLDGIEADGALKEGARERVMEELRAHFRPEFLNRVDETVVFLPLRRDQISRIVDLQLARLRRRLEDRK
IRLDMTDAARNFIGEAGYDPVYGARPLKRYVQQAVETPLARQIVGGKIRDGQRVNVDVKDEALTFTAE

Sequences:

>Translated_868_residues
MDINKFTEKAREAVSEAQSLAAGMGHQETDVEHLALALTQQENGIVPRILEQMGVQPRALSVALEGAVRKRPSVSGGGID
PTKIMITQRLATVLNDAQNEAKRMKDEYVSVDHLFAALTEVPPSTPLGEVFKEYKITRASFVAAMEDMRGGARVTSANPE
DTFEALTKYARDLVEAARQGKMDPVIGRDAEIRRVIRILSRRTKNNPVLIGEAGVGKTAIVEGLAFRIVKGDVPEGLKGR
KLFALDMGALIAGAKYRGEFEERLKAVLNEVEKSEGQTILFIDELHTIVGAGKTEGAMDAGNLLKPMLARGELHCIGATT
VDEYRKYIEKDPALERRFQPVMVEEPTVEDTISILRGLKERFEVHHGVRISDSAIVEAVMLSHRYITDRQLPDKAIDLID
EAAALIRTEIDSLPADLDEANRKVMQLEIEREALRRETDAASRERLEKLENELADLRIEQADLRKQWESEKGSINQVREI
KEQIEQTKLAIEQAERAYDLNKAAELKYSKLLELEKKLAEAENSDGSGAEGPRLLKEEVRPDDVAEIVGKWTGIPVTRLL
ESEREKLLRLPEQLHQRVVGQDEAVNAVSDAVLRARAGLSDPDRPTGSFIFLGPTGVGKTELSKALAEALFDTEDNMVRL
DMSEYMEKHSVSRLIGAPPGYVGYDEGGQLTEAVRRKPYSVILFDEIEKAHPDVFNTLLQLLDDGRLTDSQGRTVDFRNC
IVIMTSNIGSMHLLDGIEADGALKEGARERVMEELRAHFRPEFLNRVDETVVFLPLRRDQISRIVDLQLARLRRRLEDRK
IRLDMTDAARNFIGEAGYDPVYGARPLKRYVQQAVETPLARQIVGGKIRDGQRVNVDVKDEALTFTAE
>Mature_868_residues
MDINKFTEKAREAVSEAQSLAAGMGHQETDVEHLALALTQQENGIVPRILEQMGVQPRALSVALEGAVRKRPSVSGGGID
PTKIMITQRLATVLNDAQNEAKRMKDEYVSVDHLFAALTEVPPSTPLGEVFKEYKITRASFVAAMEDMRGGARVTSANPE
DTFEALTKYARDLVEAARQGKMDPVIGRDAEIRRVIRILSRRTKNNPVLIGEAGVGKTAIVEGLAFRIVKGDVPEGLKGR
KLFALDMGALIAGAKYRGEFEERLKAVLNEVEKSEGQTILFIDELHTIVGAGKTEGAMDAGNLLKPMLARGELHCIGATT
VDEYRKYIEKDPALERRFQPVMVEEPTVEDTISILRGLKERFEVHHGVRISDSAIVEAVMLSHRYITDRQLPDKAIDLID
EAAALIRTEIDSLPADLDEANRKVMQLEIEREALRRETDAASRERLEKLENELADLRIEQADLRKQWESEKGSINQVREI
KEQIEQTKLAIEQAERAYDLNKAAELKYSKLLELEKKLAEAENSDGSGAEGPRLLKEEVRPDDVAEIVGKWTGIPVTRLL
ESEREKLLRLPEQLHQRVVGQDEAVNAVSDAVLRARAGLSDPDRPTGSFIFLGPTGVGKTELSKALAEALFDTEDNMVRL
DMSEYMEKHSVSRLIGAPPGYVGYDEGGQLTEAVRRKPYSVILFDEIEKAHPDVFNTLLQLLDDGRLTDSQGRTVDFRNC
IVIMTSNIGSMHLLDGIEADGALKEGARERVMEELRAHFRPEFLNRVDETVVFLPLRRDQISRIVDLQLARLRRRLEDRK
IRLDMTDAARNFIGEAGYDPVYGARPLKRYVQQAVETPLARQIVGGKIRDGQRVNVDVKDEALTFTAE

Specific function: Part of a stress-induced multi-chaperone system, it is involved in the recovery of the cell from heat-induced damage, in cooperation with DnaK, DnaJ and GrpE. Acts before DnaK, in the processing of protein aggregates. Protein binding stimulates the ATPase

COG id: COG0542

COG function: function code O; ATPases with chaperone activity, ATP-binding subunit

Gene ontology:

Cell location: Cytoplasm (Probable) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the clpA/clpB family [H]

Homologues:

Organism=Homo sapiens, GI13540606, Length=303, Percent_Identity=37.953795379538, Blast_Score=202, Evalue=1e-51,
Organism=Escherichia coli, GI1788943, Length=862, Percent_Identity=57.1925754060325, Blast_Score=971, Evalue=0.0,
Organism=Escherichia coli, GI1787109, Length=326, Percent_Identity=44.1717791411043, Blast_Score=287, Evalue=2e-78,
Organism=Saccharomyces cerevisiae, GI6320464, Length=702, Percent_Identity=54.2735042735043, Blast_Score=772, Evalue=0.0,
Organism=Saccharomyces cerevisiae, GI6323002, Length=874, Percent_Identity=43.3638443935927, Blast_Score=677, Evalue=0.0,

Paralogues:

None

Copy number: 560 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003593
- InterPro:   IPR013093
- InterPro:   IPR003959
- InterPro:   IPR018368
- InterPro:   IPR017730
- InterPro:   IPR001270
- InterPro:   IPR019489
- InterPro:   IPR004176
- InterPro:   IPR023150 [H]

Pfam domain/function: PF00004 AAA; PF07724 AAA_2; PF02861 Clp_N; PF10431 ClpB_D2-small [H]

EC number: NA

Molecular weight: Translated: 96919; Mature: 96919

Theoretical pI: Translated: 5.05; Mature: 5.05

Prosite motif: PS00870 CLPAB_1 ; PS00871 CLPAB_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.2 %Cys     (Translated Protein)
2.4 %Met     (Translated Protein)
2.6 %Cys+Met (Translated Protein)
0.2 %Cys     (Mature Protein)
2.4 %Met     (Mature Protein)
2.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MDINKFTEKAREAVSEAQSLAAGMGHQETDVEHLALALTQQENGIVPRILEQMGVQPRAL
CCHHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHCCCCCHHHHHHHHCCCCHHH
SVALEGAVRKRPSVSGGGIDPTKIMITQRLATVLNDAQNEAKRMKDEYVSVDHLFAALTE
HHHHHHHHHCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
VPPSTPLGEVFKEYKITRASFVAAMEDMRGGARVTSANPEDTFEALTKYARDLVEAARQG
CCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCEECCCCCHHHHHHHHHHHHHHHHHHHCC
KMDPVIGRDAEIRRVIRILSRRTKNNPVLIGEAGVGKTAIVEGLAFRIVKGDVPEGLKGR
CCCCCCCCCHHHHHHHHHHHHCCCCCCEEEECCCCCHHHHHHHHHHHHCCCCCCCCCCCC
KLFALDMGALIAGAKYRGEFEERLKAVLNEVEKSEGQTILFIDELHTIVGAGKTEGAMDA
EEEEEEHHHHHHCCHHCCCHHHHHHHHHHHHHHCCCCEEEEHHHHHHHHCCCCCCCCCCH
GNLLKPMLARGELHCIGATTVDEYRKYIEKDPALERRFQPVMVEEPTVEDTISILRGLKE
HHHHHHHHHCCCEEEEECCCHHHHHHHHHCCCHHHHCCCCCEECCCCHHHHHHHHHHHHH
RFEVHHGVRISDSAIVEAVMLSHRYITDRQLPDKAIDLIDEAAALIRTEIDSLPADLDEA
HHHHHCCCCCCHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHCCCCHHHH
NRKVMQLEIEREALRRETDAASRERLEKLENELADLRIEQADLRKQWESEKGSINQVREI
CHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHCCCCCCHHHHHHH
KEQIEQTKLAIEQAERAYDLNKAAELKYSKLLELEKKLAEAENSDGSGAEGPRLLKEEVR
HHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCHHHHHHHCC
PDDVAEIVGKWTGIPVTRLLESEREKLLRLPEQLHQRVVGQDEAVNAVSDAVLRARAGLS
CHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHCCCC
DPDRPTGSFIFLGPTGVGKTELSKALAEALFDTEDNMVRLDMSEYMEKHSVSRLIGAPPG
CCCCCCCCEEEECCCCCCHHHHHHHHHHHHHCCCCCEEEECHHHHHHHHHHHHHHCCCCC
YVGYDEGGQLTEAVRRKPYSVILFDEIEKAHPDVFNTLLQLLDDGRLTDSQGRTVDFRNC
CCCCCCCCHHHHHHHCCCCEEEEEHHHHHCCHHHHHHHHHHHCCCCCCCCCCCEEECCCE
IVIMTSNIGSMHLLDGIEADGALKEGARERVMEELRAHFRPEFLNRVDETVVFLPLRRDQ
EEEEECCCCCCHHHCCCCCCCHHHHHHHHHHHHHHHHHCCHHHHHHHHHEEEEEECCHHH
ISRIVDLQLARLRRRLEDRKIRLDMTDAARNFIGEAGYDPVYGARPLKRYVQQAVETPLA
HHHHHHHHHHHHHHHHHHCEEEEEHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHHHCHHH
RQIVGGKIRDGQRVNVDVKDEALTFTAE
HHHHCCCCCCCCEEEEEECCCCEEEECC
>Mature Secondary Structure
MDINKFTEKAREAVSEAQSLAAGMGHQETDVEHLALALTQQENGIVPRILEQMGVQPRAL
CCHHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHCCCCCHHHHHHHHCCCCHHH
SVALEGAVRKRPSVSGGGIDPTKIMITQRLATVLNDAQNEAKRMKDEYVSVDHLFAALTE
HHHHHHHHHCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
VPPSTPLGEVFKEYKITRASFVAAMEDMRGGARVTSANPEDTFEALTKYARDLVEAARQG
CCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCEECCCCCHHHHHHHHHHHHHHHHHHHCC
KMDPVIGRDAEIRRVIRILSRRTKNNPVLIGEAGVGKTAIVEGLAFRIVKGDVPEGLKGR
CCCCCCCCCHHHHHHHHHHHHCCCCCCEEEECCCCCHHHHHHHHHHHHCCCCCCCCCCCC
KLFALDMGALIAGAKYRGEFEERLKAVLNEVEKSEGQTILFIDELHTIVGAGKTEGAMDA
EEEEEEHHHHHHCCHHCCCHHHHHHHHHHHHHHCCCCEEEEHHHHHHHHCCCCCCCCCCH
GNLLKPMLARGELHCIGATTVDEYRKYIEKDPALERRFQPVMVEEPTVEDTISILRGLKE
HHHHHHHHHCCCEEEEECCCHHHHHHHHHCCCHHHHCCCCCEECCCCHHHHHHHHHHHHH
RFEVHHGVRISDSAIVEAVMLSHRYITDRQLPDKAIDLIDEAAALIRTEIDSLPADLDEA
HHHHHCCCCCCHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHCCCCHHHH
NRKVMQLEIEREALRRETDAASRERLEKLENELADLRIEQADLRKQWESEKGSINQVREI
CHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHCCCCCCHHHHHHH
KEQIEQTKLAIEQAERAYDLNKAAELKYSKLLELEKKLAEAENSDGSGAEGPRLLKEEVR
HHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCHHHHHHHCC
PDDVAEIVGKWTGIPVTRLLESEREKLLRLPEQLHQRVVGQDEAVNAVSDAVLRARAGLS
CHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHCCCC
DPDRPTGSFIFLGPTGVGKTELSKALAEALFDTEDNMVRLDMSEYMEKHSVSRLIGAPPG
CCCCCCCCEEEECCCCCCHHHHHHHHHHHHHCCCCCEEEECHHHHHHHHHHHHHHCCCCC
YVGYDEGGQLTEAVRRKPYSVILFDEIEKAHPDVFNTLLQLLDDGRLTDSQGRTVDFRNC
CCCCCCCCHHHHHHHCCCCEEEEEHHHHHCCHHHHHHHHHHHCCCCCCCCCCCEEECCCE
IVIMTSNIGSMHLLDGIEADGALKEGARERVMEELRAHFRPEFLNRVDETVVFLPLRRDQ
EEEEECCCCCCHHHCCCCCCCHHHHHHHHHHHHHHHHHCCHHHHHHHHHEEEEEECCHHH
ISRIVDLQLARLRRRLEDRKIRLDMTDAARNFIGEAGYDPVYGARPLKRYVQQAVETPLA
HHHHHHHHHHHHHHHHHHCEEEEEHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHHHCHHH
RQIVGGKIRDGQRVNVDVKDEALTFTAE
HHHHCCCCCCCCEEEEEECCCCEEEECC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: Hydrolase; Acting on peptide bonds (Peptidases); Serine endopeptidases [C]

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA