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The map label for this gene is nifB [H]

Identifier: 220903851

GI number: 220903851

Start: 694769

End: 695659

Strand: Reverse

Name: nifB [H]

Synonym: Ddes_0575

Alternate gene names: 220903851

Gene position: 695659-694769 (Counterclockwise)

Preceding gene: 220903852

Following gene: 220903850

Centisome position: 24.21

GC content: 64.2

Gene sequence:

>891_bases
ATGACCATAGCCCAGGCCGACAGTGCGCGGCATCCCTGTTTCAATGCCGAGGCCCACCGCTCCTTCGGCAGGGCGCACCT
GCCTGTGGCTGCGGGCTGCAATGTGCAGTGCGGATTTTACGACCGCCGCTATTCCTGCGTCAACGAATCGCGCCCGGGCG
TTACAGCCCGGCTGCTGGAGCCGGAAGAAGCCCTTGAGGCCGCCCTGCGTGCCGTAAAGCAGATGCCGCACCTGAGCGTC
ATCGGCATTGCCGGCCCCGGCGACCCCCTGGCAGACGCCGGGCGCACACTGCACACACTGGAAGCATTGCGCAAGGCACT
GCCCCACATTCTGCTTTGTCTGTCCACCAATGGCCTTGCCCTGCCGCTGCATGCTGCGGCCCTGTCGTCTTTGGGGGTGG
GGCATGTGACAGTTACCGTCAATGCGGTTGACCCATCCATTGGCGCAGACATTTACACCTGGGTGAGCGATGGCGCGCGC
AGGCTGACGGGAACAGACGCCGCGGCCCTGCTGCTTGAACGGCAGGAAGAAGGAATACGCCGCCTCAAGGCAGCGGGGGT
AACCGTAAAAATAAACTCCGTGGTTATCCCGGGCATCAACGACAGGCACGTGCAGGCTGTCGCCCAAGCGGTGTCTTCCT
GGGGAGCAGACCTTATGAACTGCATTCCCCTGTTGCCTGTGCAGGGAACCCGGTTTGCCCGCACGCCCTCACCGGACCCG
CAAATGATGCGCCGCCTGCGGGACGAGGCCGCCATACTCATCCCCCAGATGCGCCACTGCACACGCTGCCGCGCCGATGC
GCTGGGCCTTCTTGGCGAAGAAGGACAACTGGAGGATGTCTGCCACGGGCCGCTGCCCGCACACCGGAAACTTCATGCCT
GCGGTCAGTGA

Upstream 100 bases:

>100_bases
GCACACGGCCTGATGAAGAAAACTGCACGAATTGCGGCGACTGCGTGGGATCATGCCCCAAGGACTGTATACGGTTCGGC
ATAAAACCGGGGAGCAAGGC

Downstream 100 bases:

>100_bases
AACGCCGCAGGCTTTTAAAATCTTGCATCCACCCAGACCATACGCGGTATACGCGCAGACCTGCCCGGATGCACACGGTC
AAGGTTGAATACCCTGCAAC

Product: Radical SAM domain-containing protein

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 296; Mature: 295

Protein sequence:

>296_residues
MTIAQADSARHPCFNAEAHRSFGRAHLPVAAGCNVQCGFYDRRYSCVNESRPGVTARLLEPEEALEAALRAVKQMPHLSV
IGIAGPGDPLADAGRTLHTLEALRKALPHILLCLSTNGLALPLHAAALSSLGVGHVTVTVNAVDPSIGADIYTWVSDGAR
RLTGTDAAALLLERQEEGIRRLKAAGVTVKINSVVIPGINDRHVQAVAQAVSSWGADLMNCIPLLPVQGTRFARTPSPDP
QMMRRLRDEAAILIPQMRHCTRCRADALGLLGEEGQLEDVCHGPLPAHRKLHACGQ

Sequences:

>Translated_296_residues
MTIAQADSARHPCFNAEAHRSFGRAHLPVAAGCNVQCGFYDRRYSCVNESRPGVTARLLEPEEALEAALRAVKQMPHLSV
IGIAGPGDPLADAGRTLHTLEALRKALPHILLCLSTNGLALPLHAAALSSLGVGHVTVTVNAVDPSIGADIYTWVSDGAR
RLTGTDAAALLLERQEEGIRRLKAAGVTVKINSVVIPGINDRHVQAVAQAVSSWGADLMNCIPLLPVQGTRFARTPSPDP
QMMRRLRDEAAILIPQMRHCTRCRADALGLLGEEGQLEDVCHGPLPAHRKLHACGQ
>Mature_295_residues
TIAQADSARHPCFNAEAHRSFGRAHLPVAAGCNVQCGFYDRRYSCVNESRPGVTARLLEPEEALEAALRAVKQMPHLSVI
GIAGPGDPLADAGRTLHTLEALRKALPHILLCLSTNGLALPLHAAALSSLGVGHVTVTVNAVDPSIGADIYTWVSDGARR
LTGTDAAALLLERQEEGIRRLKAAGVTVKINSVVIPGINDRHVQAVAQAVSSWGADLMNCIPLLPVQGTRFARTPSPDPQ
MMRRLRDEAAILIPQMRHCTRCRADALGLLGEEGQLEDVCHGPLPAHRKLHACGQ

Specific function: Probably involved in the synthesis of the Fe-Mo cofactor [H]

COG id: COG0535

COG function: function code R; Predicted Fe-S oxidoreductases

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Belongs to the moaA/nifB/pqqE family [H]

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003731
- InterPro:   IPR006638
- InterPro:   IPR000385
- InterPro:   IPR005980
- InterPro:   IPR007197 [H]

Pfam domain/function: PF02579 Nitro_FeMo-Co; PF04055 Radical_SAM [H]

EC number: NA

Molecular weight: Translated: 31634; Mature: 31503

Theoretical pI: Translated: 7.96; Mature: 7.96

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

3.4 %Cys     (Translated Protein)
2.0 %Met     (Translated Protein)
5.4 %Cys+Met (Translated Protein)
3.4 %Cys     (Mature Protein)
1.7 %Met     (Mature Protein)
5.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTIAQADSARHPCFNAEAHRSFGRAHLPVAAGCNVQCGFYDRRYSCVNESRPGVTARLLE
CCCCCCCCCCCCCCCCHHHHCCCCCCCCEECCCCEEECCCHHHHHHHCCCCCCCEEEECC
PEEALEAALRAVKQMPHLSVIGIAGPGDPLADAGRTLHTLEALRKALPHILLCLSTNGLA
HHHHHHHHHHHHHHCCCEEEEEECCCCCCHHHCCHHHHHHHHHHHHHHHEEEEECCCCCE
LPLHAAALSSLGVGHVTVTVNAVDPSIGADIYTWVSDGARRLTGTDAAALLLERQEEGIR
EHHHHHHHHHCCCCEEEEEEEECCCCCCCHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHH
RLKAAGVTVKINSVVIPGINDRHVQAVAQAVSSWGADLMNCIPLLPVQGTRFARTPSPDP
HHHHCCEEEEECCEEECCCCHHHHHHHHHHHHHHCHHHHHHHHCCCCCCCCCCCCCCCCH
QMMRRLRDEAAILIPQMRHCTRCRADALGLLGEEGQLEDVCHGPLPAHRKLHACGQ
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHCCCCCCHHHHHCCCC
>Mature Secondary Structure 
TIAQADSARHPCFNAEAHRSFGRAHLPVAAGCNVQCGFYDRRYSCVNESRPGVTARLLE
CCCCCCCCCCCCCCCHHHHCCCCCCCCEECCCCEEECCCHHHHHHHCCCCCCCEEEECC
PEEALEAALRAVKQMPHLSVIGIAGPGDPLADAGRTLHTLEALRKALPHILLCLSTNGLA
HHHHHHHHHHHHHHCCCEEEEEECCCCCCHHHCCHHHHHHHHHHHHHHHEEEEECCCCCE
LPLHAAALSSLGVGHVTVTVNAVDPSIGADIYTWVSDGARRLTGTDAAALLLERQEEGIR
EHHHHHHHHHCCCCEEEEEEEECCCCCCCHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHH
RLKAAGVTVKINSVVIPGINDRHVQAVAQAVSSWGADLMNCIPLLPVQGTRFARTPSPDP
HHHHCCEEEEECCEEECCCCHHHHHHHHHHHHHHCHHHHHHHHCCCCCCCCCCCCCCCCH
QMMRRLRDEAAILIPQMRHCTRCRADALGLLGEEGQLEDVCHGPLPAHRKLHACGQ
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHCCCCCCHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 7496536 [H]