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The map label for this gene is rlmE [H]

Identifier: 220903814

GI number: 220903814

Start: 647657

End: 648328

Strand: Reverse

Name: rlmE [H]

Synonym: Ddes_0537

Alternate gene names: 220903814

Gene position: 648328-647657 (Counterclockwise)

Preceding gene: 220903820

Following gene: 220903813

Centisome position: 22.56

GC content: 58.78

Gene sequence:

>672_bases
ATGCACAAAAAGGAGTACGCTTGCGCGTTGGTATTTATTATGAAGGAATATCGAGACCATTACTTCCTCAAGGCCAAGCG
CGAGAACTATCCCGCGCGTTCGGTCTACAAGCTCAAGGAACTGGACGCCAAATTCCGCCTGTTGCGGCCCGGCCAAAAGA
TTCTGGACCTGGGGGCAGCCCCCGGATCCTGGTCATTGGGCGCGGCGGAAAAGGTGGGTTCCAGGGGCCTGGTCCTTGCC
TGTGACATTCAAAGCACCGAGACGGCCTTTCCCCCCCAGGTCATTTTCATGCAGGAAGACGTGTTCAACCGCTCCCCGGC
CTTTGAGGCCAGGCTGGAAGAACTCGGCCCCTTTGACCATGTCATAAGCGACATGGCCCCGCGCACCACAGGCACCCGCT
TTACCGACCAGGCCCGCTCACTGGATCTGGCTGTGGAGGCGCTGGCTGTGGCCTGCCTGCATTTGAAGCAGGGCGGCGGC
TTTGTGGTGAAAATTTTCATGGGACCGGACGTGCAGGAACTGCTTGCGCCCATGCGCAAGGCTTTTACAACGGTCAAGTC
CTTCAAACCCAAGAGTTCGCGCGCCGAAAGCAAGGAAACTTTTTTTGTGGGCCTTGGCTTTCGCGGCAAGCCGAACCAGA
CCGACCCTGTGGCCACGCCGCCGGGCAGGTAA

Upstream 100 bases:

>100_bases
CCCGCCATGAAACCGTACTGATAACCTATATCATCAAAAGAATGCGGTCAACGCATCCGGAGATTTTTTCGGGTACCTGC
CGCACACGCCACTTGGCGAA

Downstream 100 bases:

>100_bases
AACAATTTATTCCCTAAAGGTTTCGGAGGTTTTATGGCTGGTCACAGTAAATGGGCCAATATTCAGCATCGTAAGGGCCG
TCAGGACGCAAAACGCGGCA

Product: ribosomal RNA methyltransferase RrmJ/FtsJ

Products: NA

Alternate protein names: 23S rRNA Um2552 methyltransferase; rRNA (uridine-2'-O-)-methyltransferase [H]

Number of amino acids: Translated: 223; Mature: 223

Protein sequence:

>223_residues
MHKKEYACALVFIMKEYRDHYFLKAKRENYPARSVYKLKELDAKFRLLRPGQKILDLGAAPGSWSLGAAEKVGSRGLVLA
CDIQSTETAFPPQVIFMQEDVFNRSPAFEARLEELGPFDHVISDMAPRTTGTRFTDQARSLDLAVEALAVACLHLKQGGG
FVVKIFMGPDVQELLAPMRKAFTTVKSFKPKSSRAESKETFFVGLGFRGKPNQTDPVATPPGR

Sequences:

>Translated_223_residues
MHKKEYACALVFIMKEYRDHYFLKAKRENYPARSVYKLKELDAKFRLLRPGQKILDLGAAPGSWSLGAAEKVGSRGLVLA
CDIQSTETAFPPQVIFMQEDVFNRSPAFEARLEELGPFDHVISDMAPRTTGTRFTDQARSLDLAVEALAVACLHLKQGGG
FVVKIFMGPDVQELLAPMRKAFTTVKSFKPKSSRAESKETFFVGLGFRGKPNQTDPVATPPGR
>Mature_223_residues
MHKKEYACALVFIMKEYRDHYFLKAKRENYPARSVYKLKELDAKFRLLRPGQKILDLGAAPGSWSLGAAEKVGSRGLVLA
CDIQSTETAFPPQVIFMQEDVFNRSPAFEARLEELGPFDHVISDMAPRTTGTRFTDQARSLDLAVEALAVACLHLKQGGG
FVVKIFMGPDVQELLAPMRKAFTTVKSFKPKSSRAESKETFFVGLGFRGKPNQTDPVATPPGR

Specific function: Specifically methylates the uridine in position 2552 of 23S rRNA at the 2'-O position of the ribose in the fully assembled 50S ribosomal subunit [H]

COG id: COG0293

COG function: function code J; 23S rRNA methylase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the methyltransferase superfamily. RlmE family [H]

Homologues:

Organism=Homo sapiens, GI29029591, Length=196, Percent_Identity=39.7959183673469, Blast_Score=115, Evalue=2e-26,
Organism=Homo sapiens, GI29029589, Length=196, Percent_Identity=39.7959183673469, Blast_Score=115, Evalue=2e-26,
Organism=Homo sapiens, GI7110661, Length=196, Percent_Identity=39.7959183673469, Blast_Score=115, Evalue=2e-26,
Organism=Homo sapiens, GI7019377, Length=213, Percent_Identity=33.3333333333333, Blast_Score=110, Evalue=8e-25,
Organism=Homo sapiens, GI194097365, Length=205, Percent_Identity=33.1707317073171, Blast_Score=102, Evalue=3e-22,
Organism=Escherichia coli, GI1789569, Length=206, Percent_Identity=36.4077669902913, Blast_Score=124, Evalue=6e-30,
Organism=Caenorhabditis elegans, GI17554650, Length=197, Percent_Identity=34.010152284264, Blast_Score=97, Evalue=6e-21,
Organism=Caenorhabditis elegans, GI17553474, Length=204, Percent_Identity=28.921568627451, Blast_Score=96, Evalue=2e-20,
Organism=Caenorhabditis elegans, GI71987550, Length=196, Percent_Identity=31.6326530612245, Blast_Score=82, Evalue=2e-16,
Organism=Caenorhabditis elegans, GI71987561, Length=196, Percent_Identity=31.6326530612245, Blast_Score=82, Evalue=3e-16,
Organism=Caenorhabditis elegans, GI17553860, Length=196, Percent_Identity=31.6326530612245, Blast_Score=81, Evalue=3e-16,
Organism=Saccharomyces cerevisiae, GI6319535, Length=213, Percent_Identity=35.2112676056338, Blast_Score=103, Evalue=3e-23,
Organism=Saccharomyces cerevisiae, GI6319796, Length=205, Percent_Identity=31.219512195122, Blast_Score=87, Evalue=3e-18,
Organism=Saccharomyces cerevisiae, GI6321302, Length=134, Percent_Identity=35.0746268656716, Blast_Score=66, Evalue=4e-12,
Organism=Drosophila melanogaster, GI21356387, Length=209, Percent_Identity=35.8851674641148, Blast_Score=110, Evalue=1e-24,
Organism=Drosophila melanogaster, GI24647580, Length=208, Percent_Identity=35.0961538461538, Blast_Score=96, Evalue=3e-20,
Organism=Drosophila melanogaster, GI24648639, Length=203, Percent_Identity=33.0049261083744, Blast_Score=88, Evalue=4e-18,
Organism=Drosophila melanogaster, GI18859957, Length=210, Percent_Identity=30.4761904761905, Blast_Score=88, Evalue=4e-18,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR015507
- InterPro:   IPR002877 [H]

Pfam domain/function: PF01728 FtsJ [H]

EC number: =2.1.1.166 [H]

Molecular weight: Translated: 24815; Mature: 24815

Theoretical pI: Translated: 9.92; Mature: 9.92

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.3 %Cys     (Translated Protein)
2.7 %Met     (Translated Protein)
4.0 %Cys+Met (Translated Protein)
1.3 %Cys     (Mature Protein)
2.7 %Met     (Mature Protein)
4.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MHKKEYACALVFIMKEYRDHYFLKAKRENYPARSVYKLKELDAKFRLLRPGQKILDLGAA
CCCHHHHHHHHHHHHHHHHHEEEEECCCCCCHHHHHHHHHHCCHHEECCCCHHHHHCCCC
PGSWSLGAAEKVGSRGLVLACDIQSTETAFPPQVIFMQEDVFNRSPAFEARLEELGPFDH
CCCCCCCHHHHCCCCCEEEEEECCCCCCCCCCEEEEEEHHHCCCCCHHHHHHHHCCCHHH
VISDMAPRTTGTRFTDQARSLDLAVEALAVACLHLKQGGGFVVKIFMGPDVQELLAPMRK
HHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEEEEECCCHHHHHHHHHH
AFTTVKSFKPKSSRAESKETFFVGLGFRGKPNQTDPVATPPGR
HHHHHHHCCCCCCCCCCCCEEEEEECCCCCCCCCCCCCCCCCC
>Mature Secondary Structure
MHKKEYACALVFIMKEYRDHYFLKAKRENYPARSVYKLKELDAKFRLLRPGQKILDLGAA
CCCHHHHHHHHHHHHHHHHHEEEEECCCCCCHHHHHHHHHHCCHHEECCCCHHHHHCCCC
PGSWSLGAAEKVGSRGLVLACDIQSTETAFPPQVIFMQEDVFNRSPAFEARLEELGPFDH
CCCCCCCHHHHCCCCCEEEEEECCCCCCCCCCEEEEEEHHHCCCCCHHHHHHHHCCCHHH
VISDMAPRTTGTRFTDQARSLDLAVEALAVACLHLKQGGGFVVKIFMGPDVQELLAPMRK
HHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEEEEECCCHHHHHHHHHH
AFTTVKSFKPKSSRAESKETFFVGLGFRGKPNQTDPVATPPGR
HHHHHHHCCCCCCCCCCCCEEEEEECCCCCCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA