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The map label for this gene is cfiB [H]

Identifier: 220903738

GI number: 220903738

Start: 555652

End: 557106

Strand: Reverse

Name: cfiB [H]

Synonym: Ddes_0460

Alternate gene names: 220903738

Gene position: 557106-555652 (Counterclockwise)

Preceding gene: 220903741

Following gene: 220903737

Centisome position: 19.39

GC content: 57.04

Gene sequence:

>1455_bases
ATGAGGGAGACAGGCTTGGACAACGCTCAGGAAACCATTCCCCTGCACAAGCACAAAATTCTGGTAGCCAATCGCGGCGA
AATAGCCATGCGCATCATGCGGGCCTGCCGCAAGCTGGGTGTGGCTTTTACCGCCATATTCACCGCCGAGGATGCGGCTT
CGGGCCATGTACGCCTTGCGCGCGAAGAAGGTGGAGAAAAAAGCCTTTTTCGCGTTTCTTCCTACCACGACGCCAACGAG
CTTATGTCTGTGGCTGACGAGGCAGGCTGCACCGCCGTGCATCCCGGCTACGGTTTTTTTGCCGAAGACTTCCGTTTTGC
CCGCCGTGTGACCAAGCGCGATCGCAAACTCGTCTTTATCGGCCCTTCGTGGAAGATCATCCGTGAACTGGGCGACAAGA
TCAACACCAAGCGCCTGGCGCGCAGCCTTGGCGTGCCCACGGTTCCCGGCTCTGACCGGCCCATCTATGACGAGATGGAG
GCAGAACGCATCGCCAAAAGCATCTTTGAATTTCAAGCCCAGCAAGGCATCACCCGCCCGCTGGTGCTGGTCAAGGCTTC
GGCAGGCGGCGGCGGCATGGGCATCGAGGAAGTATACGACCCGGACCAGTTCCGCTCCGTCTACCGCCGCATCCGCAACT
ATGCCCTGCGCCAGTTCAAAGACGAAGGCGTACTCATCGAGCAGCGCATTACGGACTTTAACCACCTTGAAGTTCAGGTC
GTTTCCGACCGCACAGGAATTAATCCCGTGCATTTCGGCACGCGTAACTGCTCCATCCAGTCCACAGGGCTGCAAAAGCG
CATCGAGGTGGCCCCCGGCTTTGTTCCGGAAGAAATCAAGTACACCTTTGACGCCGGCAAGGTGCTCAAGGATATTGTCC
ATTACTCCCTGACCATGGCCCGCAAGGTCGGCTACGACAACGTGGGCACATGGGAATGGATCGTTACCCGCAAGGGCGAA
CCTTTCCTTATGGAAGTGAATACCCGCATCCAGGTGGAAAACGGCGTTTCGGCCCGCATTTCCAAGGTCAAGGGGCAGGG
CGATGTGGACATCATTGCCGAACAGATACGTATCGGCCTTGGCGATCCCCTGGGCTATGCCCAGGACGACATCACTTTTG
ACGGCGTGGGCATCGAATACCGGCTCATCGCCGAAGACCCGGACAGCAACTTTACCCCCTGGGTAGGGCGCATTGAGCGC
TTCCAGTGGAAAGAACAGCCGTGGCTGACCATGCTCACCCACGTTCCCACCCAGGAACCATACGAAATCCCGACCGAATT
TGATCCAAACCTGGCTCTTGCCATCATATGGGGCAAAGACCTTGAAGAAGCCAAGGCCCGTGGTCTTGAATTTTTGCACG
ATCTGCGCCTTGAGGGGCATAACGGCGCAGGAGACACCCTAAAATCCAACGTAAACTTTTTAGCGGCCAATACGGAACGG
ATTCTCCGTTTCTGA

Upstream 100 bases:

>100_bases
CAAACCGGCGGGCCGGACTACGCTTCGGCGACCGCGTTACGTGGCCGCGCCGGAACTATCAGACCAACAACTACCGCTTG
CGCCCCCAGGGCGCCACATA

Downstream 100 bases:

>100_bases
CCAAAGGAGCAAATCATGGGGACCCCCCAGTTTCCCACATGCGCTACCTGCCCCTTCAAGCGCGAAGACCGCCTGTGCGT
CAAAGCCGGCGGCAAGCACC

Product: Pyruvate carboxylase

Products: NA

Alternate protein names: 2-oxoglutarate carboxylase beta subunit [H]

Number of amino acids: Translated: 484; Mature: 484

Protein sequence:

>484_residues
MRETGLDNAQETIPLHKHKILVANRGEIAMRIMRACRKLGVAFTAIFTAEDAASGHVRLAREEGGEKSLFRVSSYHDANE
LMSVADEAGCTAVHPGYGFFAEDFRFARRVTKRDRKLVFIGPSWKIIRELGDKINTKRLARSLGVPTVPGSDRPIYDEME
AERIAKSIFEFQAQQGITRPLVLVKASAGGGGMGIEEVYDPDQFRSVYRRIRNYALRQFKDEGVLIEQRITDFNHLEVQV
VSDRTGINPVHFGTRNCSIQSTGLQKRIEVAPGFVPEEIKYTFDAGKVLKDIVHYSLTMARKVGYDNVGTWEWIVTRKGE
PFLMEVNTRIQVENGVSARISKVKGQGDVDIIAEQIRIGLGDPLGYAQDDITFDGVGIEYRLIAEDPDSNFTPWVGRIER
FQWKEQPWLTMLTHVPTQEPYEIPTEFDPNLALAIIWGKDLEEAKARGLEFLHDLRLEGHNGAGDTLKSNVNFLAANTER
ILRF

Sequences:

>Translated_484_residues
MRETGLDNAQETIPLHKHKILVANRGEIAMRIMRACRKLGVAFTAIFTAEDAASGHVRLAREEGGEKSLFRVSSYHDANE
LMSVADEAGCTAVHPGYGFFAEDFRFARRVTKRDRKLVFIGPSWKIIRELGDKINTKRLARSLGVPTVPGSDRPIYDEME
AERIAKSIFEFQAQQGITRPLVLVKASAGGGGMGIEEVYDPDQFRSVYRRIRNYALRQFKDEGVLIEQRITDFNHLEVQV
VSDRTGINPVHFGTRNCSIQSTGLQKRIEVAPGFVPEEIKYTFDAGKVLKDIVHYSLTMARKVGYDNVGTWEWIVTRKGE
PFLMEVNTRIQVENGVSARISKVKGQGDVDIIAEQIRIGLGDPLGYAQDDITFDGVGIEYRLIAEDPDSNFTPWVGRIER
FQWKEQPWLTMLTHVPTQEPYEIPTEFDPNLALAIIWGKDLEEAKARGLEFLHDLRLEGHNGAGDTLKSNVNFLAANTER
ILRF
>Mature_484_residues
MRETGLDNAQETIPLHKHKILVANRGEIAMRIMRACRKLGVAFTAIFTAEDAASGHVRLAREEGGEKSLFRVSSYHDANE
LMSVADEAGCTAVHPGYGFFAEDFRFARRVTKRDRKLVFIGPSWKIIRELGDKINTKRLARSLGVPTVPGSDRPIYDEME
AERIAKSIFEFQAQQGITRPLVLVKASAGGGGMGIEEVYDPDQFRSVYRRIRNYALRQFKDEGVLIEQRITDFNHLEVQV
VSDRTGINPVHFGTRNCSIQSTGLQKRIEVAPGFVPEEIKYTFDAGKVLKDIVHYSLTMARKVGYDNVGTWEWIVTRKGE
PFLMEVNTRIQVENGVSARISKVKGQGDVDIIAEQIRIGLGDPLGYAQDDITFDGVGIEYRLIAEDPDSNFTPWVGRIER
FQWKEQPWLTMLTHVPTQEPYEIPTEFDPNLALAIIWGKDLEEAKARGLEFLHDLRLEGHNGAGDTLKSNVNFLAANTER
ILRF

Specific function: This Protein Is A Component Of The Acetyl Coenzyme A Carboxylase Complex; First, Biotin Carboxylase Catalyzes The Carboxylation Of The Carrier Protein And Then The Transcarboxylase Transfers The Carboxyl Group To Form Malonyl-CoA. [C]

COG id: COG0439

COG function: function code I; Biotin carboxylase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 biotin carboxylation domain [H]

Homologues:

Organism=Homo sapiens, GI65506442, Length=455, Percent_Identity=35.8241758241758, Blast_Score=228, Evalue=9e-60,
Organism=Homo sapiens, GI189095269, Length=455, Percent_Identity=35.8241758241758, Blast_Score=228, Evalue=1e-59,
Organism=Homo sapiens, GI295821183, Length=455, Percent_Identity=35.8241758241758, Blast_Score=228, Evalue=1e-59,
Organism=Homo sapiens, GI106049528, Length=467, Percent_Identity=34.2612419700214, Blast_Score=222, Evalue=5e-58,
Organism=Homo sapiens, GI106049295, Length=467, Percent_Identity=34.2612419700214, Blast_Score=222, Evalue=5e-58,
Organism=Homo sapiens, GI106049292, Length=467, Percent_Identity=34.2612419700214, Blast_Score=222, Evalue=5e-58,
Organism=Homo sapiens, GI116805327, Length=459, Percent_Identity=32.8976034858388, Blast_Score=207, Evalue=2e-53,
Organism=Homo sapiens, GI38679977, Length=496, Percent_Identity=24.7983870967742, Blast_Score=140, Evalue=3e-33,
Organism=Homo sapiens, GI38679967, Length=496, Percent_Identity=24.7983870967742, Blast_Score=140, Evalue=3e-33,
Organism=Homo sapiens, GI38679971, Length=496, Percent_Identity=24.7983870967742, Blast_Score=140, Evalue=3e-33,
Organism=Homo sapiens, GI38679974, Length=496, Percent_Identity=24.7983870967742, Blast_Score=140, Evalue=4e-33,
Organism=Homo sapiens, GI38679960, Length=496, Percent_Identity=24.7983870967742, Blast_Score=140, Evalue=4e-33,
Organism=Homo sapiens, GI134142062, Length=498, Percent_Identity=25.7028112449799, Blast_Score=131, Evalue=2e-30,
Organism=Escherichia coli, GI1789654, Length=459, Percent_Identity=34.8583877995643, Blast_Score=217, Evalue=1e-57,
Organism=Caenorhabditis elegans, GI17567343, Length=448, Percent_Identity=33.9285714285714, Blast_Score=218, Evalue=8e-57,
Organism=Caenorhabditis elegans, GI17562816, Length=467, Percent_Identity=34.2612419700214, Blast_Score=197, Evalue=9e-51,
Organism=Caenorhabditis elegans, GI71987519, Length=466, Percent_Identity=31.9742489270386, Blast_Score=182, Evalue=4e-46,
Organism=Caenorhabditis elegans, GI133931226, Length=479, Percent_Identity=27.1398747390397, Blast_Score=137, Evalue=1e-32,
Organism=Caenorhabditis elegans, GI71997163, Length=438, Percent_Identity=26.027397260274, Blast_Score=123, Evalue=2e-28,
Organism=Caenorhabditis elegans, GI71997168, Length=438, Percent_Identity=26.027397260274, Blast_Score=122, Evalue=3e-28,
Organism=Saccharomyces cerevisiae, GI6319685, Length=452, Percent_Identity=31.1946902654867, Blast_Score=198, Evalue=2e-51,
Organism=Saccharomyces cerevisiae, GI6319695, Length=394, Percent_Identity=36.0406091370558, Blast_Score=196, Evalue=1e-50,
Organism=Saccharomyces cerevisiae, GI6321376, Length=391, Percent_Identity=36.3171355498721, Blast_Score=186, Evalue=1e-47,
Organism=Saccharomyces cerevisiae, GI6323863, Length=423, Percent_Identity=27.4231678486998, Blast_Score=135, Evalue=2e-32,
Organism=Saccharomyces cerevisiae, GI6324343, Length=446, Percent_Identity=27.1300448430493, Blast_Score=135, Evalue=2e-32,
Organism=Drosophila melanogaster, GI24652212, Length=463, Percent_Identity=34.341252699784, Blast_Score=213, Evalue=2e-55,
Organism=Drosophila melanogaster, GI24652210, Length=463, Percent_Identity=34.341252699784, Blast_Score=213, Evalue=2e-55,
Organism=Drosophila melanogaster, GI24652214, Length=463, Percent_Identity=34.341252699784, Blast_Score=213, Evalue=2e-55,
Organism=Drosophila melanogaster, GI19921944, Length=463, Percent_Identity=34.341252699784, Blast_Score=213, Evalue=2e-55,
Organism=Drosophila melanogaster, GI24652216, Length=463, Percent_Identity=34.341252699784, Blast_Score=213, Evalue=2e-55,
Organism=Drosophila melanogaster, GI281363050, Length=463, Percent_Identity=34.341252699784, Blast_Score=213, Evalue=2e-55,
Organism=Drosophila melanogaster, GI24652224, Length=463, Percent_Identity=34.341252699784, Blast_Score=213, Evalue=2e-55,
Organism=Drosophila melanogaster, GI24652222, Length=463, Percent_Identity=34.341252699784, Blast_Score=213, Evalue=2e-55,
Organism=Drosophila melanogaster, GI24652220, Length=463, Percent_Identity=34.341252699784, Blast_Score=213, Evalue=2e-55,
Organism=Drosophila melanogaster, GI24652218, Length=463, Percent_Identity=34.341252699784, Blast_Score=213, Evalue=2e-55,
Organism=Drosophila melanogaster, GI24651757, Length=458, Percent_Identity=31.6593886462882, Blast_Score=212, Evalue=4e-55,
Organism=Drosophila melanogaster, GI24651759, Length=421, Percent_Identity=29.6912114014252, Blast_Score=174, Evalue=1e-43,
Organism=Drosophila melanogaster, GI161076409, Length=492, Percent_Identity=25.4065040650407, Blast_Score=137, Evalue=2e-32,
Organism=Drosophila melanogaster, GI24586458, Length=492, Percent_Identity=25.4065040650407, Blast_Score=136, Evalue=3e-32,
Organism=Drosophila melanogaster, GI161076407, Length=492, Percent_Identity=25.4065040650407, Blast_Score=136, Evalue=4e-32,
Organism=Drosophila melanogaster, GI24586460, Length=492, Percent_Identity=25.4065040650407, Blast_Score=136, Evalue=4e-32,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004549
- InterPro:   IPR011761
- InterPro:   IPR013815
- InterPro:   IPR013816
- InterPro:   IPR011764
- InterPro:   IPR005482
- InterPro:   IPR005479
- InterPro:   IPR005481
- InterPro:   IPR013817
- InterPro:   IPR016185
- InterPro:   IPR011054 [H]

Pfam domain/function: PF02785 Biotin_carb_C; PF00289 CPSase_L_chain; PF02786 CPSase_L_D2 [H]

EC number: =6.4.1.7 [H]

Molecular weight: Translated: 54624; Mature: 54624

Theoretical pI: Translated: 6.60; Mature: 6.60

Prosite motif: PS50975 ATP_GRASP ; PS00867 CPSASE_2 ; PS50979 BC

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
1.9 %Met     (Translated Protein)
2.5 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
1.9 %Met     (Mature Protein)
2.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRETGLDNAQETIPLHKHKILVANRGEIAMRIMRACRKLGVAFTAIFTAEDAASGHVRLA
CCCCCCCCHHHCCCCCCEEEEEECCCHHHHHHHHHHHHHCEEEEEEEECCCCCCCCEEEE
REEGGEKSLFRVSSYHDANELMSVADEAGCTAVHPGYGFFAEDFRFARRVTKRDRKLVFI
ECCCCCCCEEEECCCCCHHHHHHHHHHCCCCEECCCCCCHHHHHHHHHHHHHCCCEEEEE
GPSWKIIRELGDKINTKRLARSLGVPTVPGSDRPIYDEMEAERIAKSIFEFQAQQGITRP
CCCHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHCCCCCC
LVLVKASAGGGGMGIEEVYDPDQFRSVYRRIRNYALRQFKDEGVLIEQRITDFNHLEVQV
EEEEEECCCCCCCCHHHHCCCHHHHHHHHHHHHHHHHHHCCCCEEEEHHCCCCCCEEEEE
VSDRTGINPVHFGTRNCSIQSTGLQKRIEVAPGFVPEEIKYTFDAGKVLKDIVHYSLTMA
ECCCCCCCCEEECCCCCEEECCCCHHHHHCCCCCCCHHHEEECCHHHHHHHHHHHHHHHH
RKVGYDNVGTWEWIVTRKGEPFLMEVNTRIQVENGVSARISKVKGQGDVDIIAEQIRIGL
HHCCCCCCCCEEEEEEECCCCEEEEECCEEEECCCCHHHHHHHCCCCCHHHHHHHHHHCC
GDPLGYAQDDITFDGVGIEYRLIAEDPDSNFTPWVGRIERFQWKEQPWLTMLTHVPTQEP
CCCCCCCCCCCEECCCCEEEEEEEECCCCCCCCHHHHHHHHCCCCCCHHHHHHCCCCCCC
YEIPTEFDPNLALAIIWGKDLEEAKARGLEFLHDLRLEGHNGAGDTLKSNVNFLAANTER
CCCCCCCCCCEEEEEEECCCHHHHHHHHHHHHHHHHCCCCCCCCCHHHCCCEEEEECHHH
ILRF
HHCC
>Mature Secondary Structure
MRETGLDNAQETIPLHKHKILVANRGEIAMRIMRACRKLGVAFTAIFTAEDAASGHVRLA
CCCCCCCCHHHCCCCCCEEEEEECCCHHHHHHHHHHHHHCEEEEEEEECCCCCCCCEEEE
REEGGEKSLFRVSSYHDANELMSVADEAGCTAVHPGYGFFAEDFRFARRVTKRDRKLVFI
ECCCCCCCEEEECCCCCHHHHHHHHHHCCCCEECCCCCCHHHHHHHHHHHHHCCCEEEEE
GPSWKIIRELGDKINTKRLARSLGVPTVPGSDRPIYDEMEAERIAKSIFEFQAQQGITRP
CCCHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHCCCCCC
LVLVKASAGGGGMGIEEVYDPDQFRSVYRRIRNYALRQFKDEGVLIEQRITDFNHLEVQV
EEEEEECCCCCCCCHHHHCCCHHHHHHHHHHHHHHHHHHCCCCEEEEHHCCCCCCEEEEE
VSDRTGINPVHFGTRNCSIQSTGLQKRIEVAPGFVPEEIKYTFDAGKVLKDIVHYSLTMA
ECCCCCCCCEEECCCCCEEECCCCHHHHHCCCCCCCHHHEEECCHHHHHHHHHHHHHHHH
RKVGYDNVGTWEWIVTRKGEPFLMEVNTRIQVENGVSARISKVKGQGDVDIIAEQIRIGL
HHCCCCCCCCEEEEEEECCCCEEEEECCEEEECCCCHHHHHHHCCCCCHHHHHHHHHHCC
GDPLGYAQDDITFDGVGIEYRLIAEDPDSNFTPWVGRIERFQWKEQPWLTMLTHVPTQEP
CCCCCCCCCCCEECCCCEEEEEEEECCCCCCCCHHHHHHHHCCCCCCHHHHHHCCCCCCC
YEIPTEFDPNLALAIIWGKDLEEAKARGLEFLHDLRLEGHNGAGDTLKSNVNFLAANTER
CCCCCCCCCCEEEEEEECCCHHHHHHHHHHHHHHHHCCCCCCCCCHHHCCCEEEEECHHH
ILRF
HHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA