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The map label for this gene is hmeA [H]

Identifier: 220903726

GI number: 220903726

Start: 542332

End: 543114

Strand: Reverse

Name: hmeA [H]

Synonym: Ddes_0448

Alternate gene names: 220903726

Gene position: 543114-542332 (Counterclockwise)

Preceding gene: 220903727

Following gene: 220903725

Centisome position: 18.9

GC content: 59.9

Gene sequence:

>783_bases
ATGAACAAGAGCAGAAGAAGCTTTCTGAAAGTGGCGGGCTTTTCGGCCTTTGCCCTTACCAGCGGCATGGCTGCCCTGTC
TGGCGTGGCCCAGGCGCAGATTGCCCCCGGCAAGTATGAACGCGCGGCCAACGCCCTGCACGCCAAGCGCTGGGGCATGG
TTATCGATACGCGCCAGTTCAAGGGACCTGAAGACTACAAGCCCCTGATCGAGGCGTGCCACAGCTACCACAACGTGCCG
CACATTCCTGATAATCAGGACATCAAGTGGTTCTGGCTGGACAGCTACGCCCATGTCTTCCCTGACGACATGAACGCGCA
CCTGAACACGCACACCCGCGAATCCATGTACCCGCTTTTGTGCAACCACTGCACCAATCCGCCCTGCGTACGCGTGTGCC
CCACTCAGGCCACCTACAAAATGGACGACGGCATTGTTGCCATGGATTACCACCGCTGCATCGGCTGCCGGTTCTGCATG
GCGGGCTGTCCCTACGGCGCACGCTCGTTCAACTTCAAGGACCCGCGCAAGTTCCTGGCGGACCCTGTGCCCAATCCCGA
GTATCCCACGCGCATGATAGGCGTGGTCGAAAAATGTACCTTCTGCGCCGAGCGCCTGGCCGTGGGCCAGATGCCCGCCT
GCGTTGAAGCAGCCAATGGCAAAATTCTTTTCGGCGACCTGGAAGACCCCAACTCAACGGTGCGTCAGGCTTTGGCCACC
AACTACAGTATTCGCCGCAAGCCCAGCCTGGGCACCCAGCCCGGCGTTTACTACCTCATTTAG

Upstream 100 bases:

>100_bases
GCCACAGCAACTACAAGGAATTCTGTGAGAAGTGCCATACAGCCAACGGCGTTGATCCGTATTGCTGGACTTGCCACATC
ATTCCCCAGGGGAGCAAGTA

Downstream 100 bases:

>100_bases
GGAGAACAGGCCATGCTTGAAAAATTGCTCGACGGTCCCAAGACCTTCTACATGTGGCTGCTCTTTCTGCTCGTCGTCAT
CGCGGGTTGCGGCCTTGTGT

Product: 4Fe-4S ferredoxin iron-sulfur binding domain-containing protein

Products: oxidized cytochrome C552; NH3 [C]

Alternate protein names: Hme subunit A [H]

Number of amino acids: Translated: 260; Mature: 260

Protein sequence:

>260_residues
MNKSRRSFLKVAGFSAFALTSGMAALSGVAQAQIAPGKYERAANALHAKRWGMVIDTRQFKGPEDYKPLIEACHSYHNVP
HIPDNQDIKWFWLDSYAHVFPDDMNAHLNTHTRESMYPLLCNHCTNPPCVRVCPTQATYKMDDGIVAMDYHRCIGCRFCM
AGCPYGARSFNFKDPRKFLADPVPNPEYPTRMIGVVEKCTFCAERLAVGQMPACVEAANGKILFGDLEDPNSTVRQALAT
NYSIRRKPSLGTQPGVYYLI

Sequences:

>Translated_260_residues
MNKSRRSFLKVAGFSAFALTSGMAALSGVAQAQIAPGKYERAANALHAKRWGMVIDTRQFKGPEDYKPLIEACHSYHNVP
HIPDNQDIKWFWLDSYAHVFPDDMNAHLNTHTRESMYPLLCNHCTNPPCVRVCPTQATYKMDDGIVAMDYHRCIGCRFCM
AGCPYGARSFNFKDPRKFLADPVPNPEYPTRMIGVVEKCTFCAERLAVGQMPACVEAANGKILFGDLEDPNSTVRQALAT
NYSIRRKPSLGTQPGVYYLI
>Mature_260_residues
MNKSRRSFLKVAGFSAFALTSGMAALSGVAQAQIAPGKYERAANALHAKRWGMVIDTRQFKGPEDYKPLIEACHSYHNVP
HIPDNQDIKWFWLDSYAHVFPDDMNAHLNTHTRESMYPLLCNHCTNPPCVRVCPTQATYKMDDGIVAMDYHRCIGCRFCM
AGCPYGARSFNFKDPRKFLADPVPNPEYPTRMIGVVEKCTFCAERLAVGQMPACVEAANGKILFGDLEDPNSTVRQALAT
NYSIRRKPSLGTQPGVYYLI

Specific function: Has menaquinol-oxidizing activity. HmeA, hmeB and hmeE subunits may together catalyze electron transfer from menaquinol to cytochrome c [H]

COG id: COG0437

COG function: function code C; Fe-S-cluster-containing hydrogenase components 1

Gene ontology:

Cell location: Cell membrane; Peripheral membrane protein; Extracellular side [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 4Fe-4S ferredoxin-type domain [H]

Homologues:

Organism=Escherichia coli, GI2367345, Length=260, Percent_Identity=32.3076923076923, Blast_Score=100, Evalue=1e-22,
Organism=Escherichia coli, GI1787740, Length=129, Percent_Identity=36.4341085271318, Blast_Score=82, Evalue=3e-17,
Organism=Escherichia coli, GI1787478, Length=130, Percent_Identity=38.4615384615385, Blast_Score=82, Evalue=3e-17,
Organism=Escherichia coli, GI1787872, Length=112, Percent_Identity=37.5, Blast_Score=80, Evalue=2e-16,
Organism=Escherichia coli, GI1787122, Length=181, Percent_Identity=28.7292817679558, Blast_Score=79, Evalue=3e-16,
Organism=Escherichia coli, GI226510944, Length=233, Percent_Identity=28.3261802575107, Blast_Score=79, Evalue=3e-16,
Organism=Escherichia coli, GI1790326, Length=121, Percent_Identity=34.7107438016529, Blast_Score=75, Evalue=3e-15,
Organism=Escherichia coli, GI1789370, Length=141, Percent_Identity=32.6241134751773, Blast_Score=74, Evalue=8e-15,
Organism=Escherichia coli, GI1787749, Length=121, Percent_Identity=34.7107438016529, Blast_Score=74, Evalue=1e-14,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR017896
- InterPro:   IPR017900
- InterPro:   IPR006311 [H]

Pfam domain/function: NA

EC number: NA

Molecular weight: Translated: 29107; Mature: 29107

Theoretical pI: Translated: 8.42; Mature: 8.42

Prosite motif: PS00198 4FE4S_FERREDOXIN

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

4.6 %Cys     (Translated Protein)
3.8 %Met     (Translated Protein)
8.5 %Cys+Met (Translated Protein)
4.6 %Cys     (Mature Protein)
3.8 %Met     (Mature Protein)
8.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNKSRRSFLKVAGFSAFALTSGMAALSGVAQAQIAPGKYERAANALHAKRWGMVIDTRQF
CCCHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHCCEEEEHHCC
KGPEDYKPLIEACHSYHNVPHIPDNQDIKWFWLDSYAHVFPDDMNAHLNTHTRESMYPLL
CCCCCHHHHHHHHHHHCCCCCCCCCCCEEEEEECCCHHHCCCCCCCCCCCCCHHHHHHHH
CNHCTNPPCVRVCPTQATYKMDDGIVAMDYHRCIGCRFCMAGCPYGARSFNFKDPRKFLA
HHCCCCCCEEEECCCCCEEEECCCEEEEHHHHHHHHHHHHHCCCCCCCCCCCCCCHHHHC
DPVPNPEYPTRMIGVVEKCTFCAERLAVGQMPACVEAANGKILFGDLEDPNSTVRQALAT
CCCCCCCCCHHHHHHHHHHHHHHHHHHHCCCCHHEECCCCEEEEECCCCCHHHHHHHHHH
NYSIRRKPSLGTQPGVYYLI
CCCEEECCCCCCCCCEEEEC
>Mature Secondary Structure
MNKSRRSFLKVAGFSAFALTSGMAALSGVAQAQIAPGKYERAANALHAKRWGMVIDTRQF
CCCHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHCCEEEEHHCC
KGPEDYKPLIEACHSYHNVPHIPDNQDIKWFWLDSYAHVFPDDMNAHLNTHTRESMYPLL
CCCCCHHHHHHHHHHHCCCCCCCCCCCEEEEEECCCHHHCCCCCCCCCCCCCHHHHHHHH
CNHCTNPPCVRVCPTQATYKMDDGIVAMDYHRCIGCRFCMAGCPYGARSFNFKDPRKFLA
HHCCCCCCEEEECCCCCEEEECCCEEEEHHHHHHHHHHHHHCCCCCCCCCCCCCCHHHHC
DPVPNPEYPTRMIGVVEKCTFCAERLAVGQMPACVEAANGKILFGDLEDPNSTVRQALAT
CCCCCCCCCHHHHHHHHHHHHHHHHHHHCCCCHHEECCCCEEEEECCCCCHHHHHHHHHH
NYSIRRKPSLGTQPGVYYLI
CCCEEECCCCCCCCCEEEEC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: Fe [C]

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: reduced cytochrome C552; nitrite [C]

Specific reaction: reduced cytochrome C552 + nitrite = oxidized cytochrome C552 + NH3 [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: 9389475; 11952791 [H]