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The map label for this gene is pleD [H]

Identifier: 220903700

GI number: 220903700

Start: 513876

End: 514829

Strand: Direct

Name: pleD [H]

Synonym: Ddes_0422

Alternate gene names: 220903700

Gene position: 513876-514829 (Clockwise)

Preceding gene: 220903698

Following gene: 220903708

Centisome position: 17.88

GC content: 57.44

Gene sequence:

>954_bases
ATGGCCGAAAATGGGGACAGCGCTTTTGTTGCCCGCGACGGAAAGTCGCCGGTAGAGGATGTGATAAGTTATGTGCATGC
GGTTTTGAGCCGCAGAGTGATCGAGTGCGACGAGATTCCTGAAAATATCCGCCATGCGGGCGGCATGGAGCAGCTTTGTG
AACTGCTTTGGGGCATACGCCACATGACGGACGCCCTGGGCAGGGGAGAACTGACTTACAACTGCAATGAAAGAGGCTAT
GTTATCGGCTCACTCAAGGCGCTACAGGCCAACCTGCGCCACCTGACGTGGCAGGCCCGCTGCATCGCCAAAGGAGAATA
CCAGCACCGGGCCAATTTTCTTGGCGATTTTTCCACGGCTTTCAACACAATGGTTGAGGAGCTGGACAAGAGCGTGTCCA
AACTCACCCGCCTTTCCTCCAGATACAAGGAAATGTCCTGCCGTGATTCCCTGACAGGCCTGCTGAACCGCCGCGCCTTT
ATCAGCCGGGCCATGGACATGCTGAAAGATCCGCCGGACCAGGGGCAGGCCGCCGTTATCATGGCCGATATCGACTACTT
TAAAAAAATCAACGACACGTGGGGGCATGCCTGTGGCGATGCGGTGCTGCGTCACATGGCCCAGATATTTCTTTCGCAGC
TGCGCGAAGATGACCTGTGCTGCCGGTACGGCGGGGAGGAATTCATCCTGCTTTTGCCCGGCATGGGACTGCAGCCGGGC
TACAGTGTGGCGGAACGGTTGCGGCGGCATATCTGCGCGGAGCCGCTGGCGTACGAGGGGCATGACATTGCCATTACGGC
CAGCCTGGGGGTGAGTTGCATCGACCTCAACGGGCCGCTGCCGGACAGTGCTTCTGTTTTTGAGATGACGGTCAAAGAGG
CCGACTCCTGCCTGTATCGGGCCAAAAAAACCGGCAGAAACCGTGTTGTTTGCAGTGAAATGCATCAGGCCTGA

Upstream 100 bases:

>100_bases
GGGCGCTGCCGCATATGTGCGGAAACCAGGCACGGCATCCGGACTGTTCTGTAATGATCTGTGCGAAATACAATAGGGCA
GATCTGCAAGGTGGTGTAGC

Downstream 100 bases:

>100_bases
TCTTCGGGCAAAGTGTGCCTGAAGCGCCGATGGTTCGGCGGCGCACGCCGCTGACGCCGGAAACAGGATTTTTTAAACGC
CTGCCAAGGCCTTGCGCAGG

Product: diguanylate cyclase

Products: NA

Alternate protein names: Stalked cell differentiation-controlling protein; Diguanylate cyclase; DGC; Diguanylate kinase [H]

Number of amino acids: Translated: 317; Mature: 316

Protein sequence:

>317_residues
MAENGDSAFVARDGKSPVEDVISYVHAVLSRRVIECDEIPENIRHAGGMEQLCELLWGIRHMTDALGRGELTYNCNERGY
VIGSLKALQANLRHLTWQARCIAKGEYQHRANFLGDFSTAFNTMVEELDKSVSKLTRLSSRYKEMSCRDSLTGLLNRRAF
ISRAMDMLKDPPDQGQAAVIMADIDYFKKINDTWGHACGDAVLRHMAQIFLSQLREDDLCCRYGGEEFILLLPGMGLQPG
YSVAERLRRHICAEPLAYEGHDIAITASLGVSCIDLNGPLPDSASVFEMTVKEADSCLYRAKKTGRNRVVCSEMHQA

Sequences:

>Translated_317_residues
MAENGDSAFVARDGKSPVEDVISYVHAVLSRRVIECDEIPENIRHAGGMEQLCELLWGIRHMTDALGRGELTYNCNERGY
VIGSLKALQANLRHLTWQARCIAKGEYQHRANFLGDFSTAFNTMVEELDKSVSKLTRLSSRYKEMSCRDSLTGLLNRRAF
ISRAMDMLKDPPDQGQAAVIMADIDYFKKINDTWGHACGDAVLRHMAQIFLSQLREDDLCCRYGGEEFILLLPGMGLQPG
YSVAERLRRHICAEPLAYEGHDIAITASLGVSCIDLNGPLPDSASVFEMTVKEADSCLYRAKKTGRNRVVCSEMHQA
>Mature_316_residues
AENGDSAFVARDGKSPVEDVISYVHAVLSRRVIECDEIPENIRHAGGMEQLCELLWGIRHMTDALGRGELTYNCNERGYV
IGSLKALQANLRHLTWQARCIAKGEYQHRANFLGDFSTAFNTMVEELDKSVSKLTRLSSRYKEMSCRDSLTGLLNRRAFI
SRAMDMLKDPPDQGQAAVIMADIDYFKKINDTWGHACGDAVLRHMAQIFLSQLREDDLCCRYGGEEFILLLPGMGLQPGY
SVAERLRRHICAEPLAYEGHDIAITASLGVSCIDLNGPLPDSASVFEMTVKEADSCLYRAKKTGRNRVVCSEMHQA

Specific function: Catalyzes the condensation of two GTP molecules to the cyclic dinucleotide di-GMP (c-di-GMP), which acts as a secondary messenger [H]

COG id: COG2199

COG function: function code T; FOG: GGDEF domain

Gene ontology:

Cell location: Cytoplasm. Note=Phosphorylated PleD localizes to the differentiating pole [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 2 response regulatory domains [H]

Homologues:

Organism=Escherichia coli, GI87082007, Length=186, Percent_Identity=40.3225806451613, Blast_Score=113, Evalue=1e-26,
Organism=Escherichia coli, GI145693134, Length=228, Percent_Identity=33.7719298245614, Blast_Score=109, Evalue=2e-25,
Organism=Escherichia coli, GI1787262, Length=176, Percent_Identity=35.2272727272727, Blast_Score=103, Evalue=1e-23,
Organism=Escherichia coli, GI87081881, Length=217, Percent_Identity=32.258064516129, Blast_Score=99, Evalue=5e-22,
Organism=Escherichia coli, GI1788381, Length=195, Percent_Identity=33.8461538461538, Blast_Score=96, Evalue=3e-21,
Organism=Escherichia coli, GI1787802, Length=182, Percent_Identity=35.1648351648352, Blast_Score=96, Evalue=3e-21,
Organism=Escherichia coli, GI1786584, Length=184, Percent_Identity=32.0652173913043, Blast_Score=92, Evalue=4e-20,
Organism=Escherichia coli, GI1787816, Length=164, Percent_Identity=34.1463414634146, Blast_Score=82, Evalue=6e-17,
Organism=Escherichia coli, GI1787541, Length=187, Percent_Identity=29.4117647058824, Blast_Score=81, Evalue=1e-16,
Organism=Escherichia coli, GI1788085, Length=188, Percent_Identity=28.7234042553192, Blast_Score=80, Evalue=1e-16,
Organism=Escherichia coli, GI1788956, Length=205, Percent_Identity=29.7560975609756, Blast_Score=71, Evalue=8e-14,
Organism=Escherichia coli, GI1787056, Length=180, Percent_Identity=30, Blast_Score=66, Evalue=3e-12,
Organism=Escherichia coli, GI87081974, Length=176, Percent_Identity=27.8409090909091, Blast_Score=65, Evalue=8e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001054
- InterPro:   IPR011006
- InterPro:   IPR000160
- InterPro:   IPR001789 [H]

Pfam domain/function: PF00990 GGDEF; PF00072 Response_reg [H]

EC number: =2.7.7.65 [H]

Molecular weight: Translated: 35429; Mature: 35298

Theoretical pI: Translated: 6.34; Mature: 6.34

Prosite motif: PS50887 GGDEF

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

3.8 %Cys     (Translated Protein)
3.8 %Met     (Translated Protein)
7.6 %Cys+Met (Translated Protein)
3.8 %Cys     (Mature Protein)
3.5 %Met     (Mature Protein)
7.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAENGDSAFVARDGKSPVEDVISYVHAVLSRRVIECDEIPENIRHAGGMEQLCELLWGIR
CCCCCCCEEEECCCCCCHHHHHHHHHHHHHHHHHCHHHHHHHHHHCCCHHHHHHHHHHHH
HMTDALGRGELTYNCNERGYVIGSLKALQANLRHLTWQARCIAKGEYQHRANFLGDFSTA
HHHHHHCCCCEEEECCCCCEEEEHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHH
FNTMVEELDKSVSKLTRLSSRYKEMSCRDSLTGLLNRRAFISRAMDMLKDPPDQGQAAVI
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCEEEE
MADIDYFKKINDTWGHACGDAVLRHMAQIFLSQLREDDLCCRYGGEEFILLLPGMGLQPG
EECHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHCCCCEEEEEECCCCCCCC
YSVAERLRRHICAEPLAYEGHDIAITASLGVSCIDLNGPLPDSASVFEMTVKEADSCLYR
HHHHHHHHHHHCCCCCCCCCCCEEEEECCCEEEEECCCCCCCCCHHHHHHHHHHHHHHHH
AKKTGRNRVVCSEMHQA
HHHCCCCCEEHHHHCCC
>Mature Secondary Structure 
AENGDSAFVARDGKSPVEDVISYVHAVLSRRVIECDEIPENIRHAGGMEQLCELLWGIR
CCCCCCEEEECCCCCCHHHHHHHHHHHHHHHHHCHHHHHHHHHHCCCHHHHHHHHHHHH
HMTDALGRGELTYNCNERGYVIGSLKALQANLRHLTWQARCIAKGEYQHRANFLGDFSTA
HHHHHHCCCCEEEECCCCCEEEEHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHH
FNTMVEELDKSVSKLTRLSSRYKEMSCRDSLTGLLNRRAFISRAMDMLKDPPDQGQAAVI
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCEEEE
MADIDYFKKINDTWGHACGDAVLRHMAQIFLSQLREDDLCCRYGGEEFILLLPGMGLQPG
EECHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHCCCCEEEEEECCCCCCCC
YSVAERLRRHICAEPLAYEGHDIAITASLGVSCIDLNGPLPDSASVFEMTVKEADSCLYR
HHHHHHHHHHHCCCCCCCCCCCEEEEECCCEEEEECCCCCCCCCHHHHHHHHHHHHHHHH
AKKTGRNRVVCSEMHQA
HHHCCCCCEEHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA