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The map label for this gene is fliA [H]

Identifier: 220903606

GI number: 220903606

Start: 396936

End: 397718

Strand: Reverse

Name: fliA [H]

Synonym: Ddes_0325

Alternate gene names: 220903606

Gene position: 397718-396936 (Counterclockwise)

Preceding gene: 220903607

Following gene: 220903605

Centisome position: 13.84

GC content: 58.11

Gene sequence:

>783_bases
ATGAAAACCGGAACAGCTCAAGCGCATACCCCCGGCCCCTGGGAAGCGCTCGAAACCGGGGCAACCGCCTGGGAAAATTT
TTCTCCGGCAGAGCAGGAGGCCGTGGTGCGTCATTATGCGCCCAAAATCCGCTTTCTGGCCCTGCGTCTCAAGGCCAAGC
TGCCGCGCAGCGTGGAACTGGGCGAACTCATCAGCTCCGGAACGCTCGGCCTTATGGAGGCTCTGGGCAAGTTCCGGCCA
CAACTGGGCATACGCTTTGAAACCTACGCGGAAAGCCGCATACGCGGCGCTATGCTGGACGAATTGCGCAGGCTGGACTG
GTTCCCCCGCTCTTTGCGGCAACGGGTGCGCACGCTTGATGAAGCCATCCGCAAGGTGGAGCACGAGCATGGCCGCCAAG
CCACGGAAGAAGAGCTTCAGGCCATTACCGGCCTTCCCCTGCGGGACGTGCGCCAGGGGCTTGAAGCACTGCAAAACCAG
CTCTGGCTTTCACTGGACGCCATACAGGACACCATCTCGGGTGATGCCCAGGAACCCGGAGGAGAACCCTACCGCAAAAC
AGCCTTTCGTGAGTTGGTGGAAAGGGTTGCCCCTCTTATAGATCGCTTGACGCCAAGAGAAAAGCTGGTACTGTCACTCT
ACTATACTGATGAGTTGAATATGCGTGAAACAGCTGAAGTCATGGGCATCACAGAAGGCCGTGTTTCACAGTTGCATTCA
CAGGCCCTGAGCCGCCTGCGCAAAGAATTCGTCAGCCTGTATGGTGAGGACGCTGACATATAA

Upstream 100 bases:

>100_bases
AATAACGCGTCCTGCACAGCCTGGCGGGCAAGGGCGGCGGCGCACAACCGGCATTCAGCATGCCACGGGGTGGAAGAGGC
AAAACCGGAAGGATAGCGAC

Downstream 100 bases:

>100_bases
CGATGCAACGGGCAGGCGCGAACATCGCGGCAAGCCCCGCTACGGCGCCCCGCAGGCGTTAAGGAGCTTTCAACATGCCT
TACAATCCCAATATGCGTGT

Product: RNA polymerase sigma-28 subunit FliA/WhiG

Products: NA

Alternate protein names: Sigma-27; Sigma-28; Sigma-F factor [H]

Number of amino acids: Translated: 260; Mature: 260

Protein sequence:

>260_residues
MKTGTAQAHTPGPWEALETGATAWENFSPAEQEAVVRHYAPKIRFLALRLKAKLPRSVELGELISSGTLGLMEALGKFRP
QLGIRFETYAESRIRGAMLDELRRLDWFPRSLRQRVRTLDEAIRKVEHEHGRQATEEELQAITGLPLRDVRQGLEALQNQ
LWLSLDAIQDTISGDAQEPGGEPYRKTAFRELVERVAPLIDRLTPREKLVLSLYYTDELNMRETAEVMGITEGRVSQLHS
QALSRLRKEFVSLYGEDADI

Sequences:

>Translated_260_residues
MKTGTAQAHTPGPWEALETGATAWENFSPAEQEAVVRHYAPKIRFLALRLKAKLPRSVELGELISSGTLGLMEALGKFRP
QLGIRFETYAESRIRGAMLDELRRLDWFPRSLRQRVRTLDEAIRKVEHEHGRQATEEELQAITGLPLRDVRQGLEALQNQ
LWLSLDAIQDTISGDAQEPGGEPYRKTAFRELVERVAPLIDRLTPREKLVLSLYYTDELNMRETAEVMGITEGRVSQLHS
QALSRLRKEFVSLYGEDADI
>Mature_260_residues
MKTGTAQAHTPGPWEALETGATAWENFSPAEQEAVVRHYAPKIRFLALRLKAKLPRSVELGELISSGTLGLMEALGKFRP
QLGIRFETYAESRIRGAMLDELRRLDWFPRSLRQRVRTLDEAIRKVEHEHGRQATEEELQAITGLPLRDVRQGLEALQNQ
LWLSLDAIQDTISGDAQEPGGEPYRKTAFRELVERVAPLIDRLTPREKLVLSLYYTDELNMRETAEVMGITEGRVSQLHS
QALSRLRKEFVSLYGEDADI

Specific function: Sigma factors are initiation factors that promote the attachment of RNA polymerase to specific initiation sites and are then released. This alternative sigma factor is specific for class 3 flagellar operons [H]

COG id: COG1191

COG function: function code K; DNA-directed RNA polymerase specialized sigma subunit

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the sigma-70 factor family [H]

Homologues:

Organism=Escherichia coli, GI1788231, Length=230, Percent_Identity=41.304347826087, Blast_Score=159, Evalue=1e-40,

Paralogues:

None

Copy number: 10-20 (rich media) [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR014284
- InterPro:   IPR000943
- InterPro:   IPR007627
- InterPro:   IPR007624
- InterPro:   IPR007630
- InterPro:   IPR012845
- InterPro:   IPR013325
- InterPro:   IPR013324
- InterPro:   IPR011991 [H]

Pfam domain/function: PF04542 Sigma70_r2; PF04539 Sigma70_r3; PF04545 Sigma70_r4 [H]

EC number: NA

Molecular weight: Translated: 29546; Mature: 29546

Theoretical pI: Translated: 6.08; Mature: 6.08

Prosite motif: PS00715 SIGMA70_1 ; PS00716 SIGMA70_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
1.9 %Met     (Translated Protein)
1.9 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
1.9 %Met     (Mature Protein)
1.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKTGTAQAHTPGPWEALETGATAWENFSPAEQEAVVRHYAPKIRFLALRLKAKLPRSVEL
CCCCCCCCCCCCCHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHH
GELISSGTLGLMEALGKFRPQLGIRFETYAESRIRGAMLDELRRLDWFPRSLRQRVRTLD
HHHHHCCCHHHHHHHHHHCHHHCCCHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHH
EAIRKVEHEHGRQATEEELQAITGLPLRDVRQGLEALQNQLWLSLDAIQDTISGDAQEPG
HHHHHHHHHHCCCHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCC
GEPYRKTAFRELVERVAPLIDRLTPREKLVLSLYYTDELNMRETAEVMGITEGRVSQLHS
CCHHHHHHHHHHHHHHHHHHHHCCCHHHHEEEEEECCCCCHHHHHHHHCCCHHHHHHHHH
QALSRLRKEFVSLYGEDADI
HHHHHHHHHHHHHHCCCCCC
>Mature Secondary Structure
MKTGTAQAHTPGPWEALETGATAWENFSPAEQEAVVRHYAPKIRFLALRLKAKLPRSVEL
CCCCCCCCCCCCCHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHH
GELISSGTLGLMEALGKFRPQLGIRFETYAESRIRGAMLDELRRLDWFPRSLRQRVRTLD
HHHHHCCCHHHHHHHHHHCHHHCCCHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHH
EAIRKVEHEHGRQATEEELQAITGLPLRDVRQGLEALQNQLWLSLDAIQDTISGDAQEPG
HHHHHHHHHHCCCHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCC
GEPYRKTAFRELVERVAPLIDRLTPREKLVLSLYYTDELNMRETAEVMGITEGRVSQLHS
CCHHHHHHHHHHHHHHHHHHHHCCCHHHHEEEEEECCCCCHHHHHHHHCCCHHHHHHHHH
QALSRLRKEFVSLYGEDADI
HHHHHHHHHHHHHHCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]