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The map label for this gene is topA [H]

Identifier: 220903585

GI number: 220903585

Start: 370412

End: 372658

Strand: Direct

Name: topA [H]

Synonym: Ddes_0304

Alternate gene names: 220903585

Gene position: 370412-372658 (Clockwise)

Preceding gene: 220903582

Following gene: 220903586

Centisome position: 12.89

GC content: 59.55

Gene sequence:

>2247_bases
ATGGGCAAACAGTTGATAATAGTGGAATCACCGGCCAAGGTTAAGACCATCAAGAAATTTCTGGGACCGGCTTATATGGT
GCAGGCCAGTGTGGGCCATGTACGCGACTTGCCCGCCAAAACGCTTGGCGTGGACGAAGAGCATGACTTTGCGCCTCATT
ATGAAGTTATAGAAAATAAAAAAAATGTGGTCAGCGACCTGCGCGCCGCCGCATCCAAGGCCGAGACCGTATATCTGGCC
CCAGACCCCGACCGCGAAGGTGAGGCCATTGCCTGGCATGTGGCCGAGCTGATCCGCGACAAGGCCAAGGATATCAAGCG
TATCCAGTTCAACGAAATTACGGCCAAGGCCGTCAAGGAAGCGTTAGCGCACCCGCGCGAACTCAATGCCGACCTTTTTG
ACGCCCAGCAGGCCCGCCGTATCCTGGACAGGCTTGTGGGCTACAAGATTTCACCCCTGCTATGGAAAACCATCAAGCGC
GGTATATCCGCAGGCCGTGTACAATCCGTGGCCCTGCGCCTTATTGTGGAGCGTGAGGAAGAGCGTGAGGTCTTCAGGCC
AGAGGAATACTGGCTGTTCAAGGCCCTGCTTTCGGGCGAGGTGCCGCCTCCATTTAAGGCCGATCTGCTCAAGGTCAACG
GCAAGAAGGCGGTCATCGGCAATGCCGAAGAGGCGCAGGCTCTTGAGGCCGCCCTCAAGGGACAGCCCTTTGTGGTGGAA
AATGTGGAACAGAAGGAGCGCGAGCGCGCGCCCCAGCCGCCGTTCATCACCTCCACGCTCCAGCAGGCGGCCAACCAGCG
TCTTTCCTATTCGGCCAAGCGCACTATGAATATTGCCCAGCGCCTCTATGAAGGCGTGGAGCTGGGCGACCGCGGCCTTA
CCGCCCTTATCACCTATATGCGTACGGACTCCACGCGCATTGCCGATGAGGCCCGGGACGCGGCCAAGAAATTTATTGGC
GAACACTTCGGCAAGGATTACCTGCCCAAGCGCGCCCGTGTGTACAAGACCAAGGGCAGCGCCCAGGACGCCCACGAAGC
CATCCGCCCCGTGGATGTGTCCATCACGCCCGATGAGGTCAAGCAACACCTGCCCCCTGACCAGTACAATCTTTACCGGC
TTATCTGGTCGCGCTTTGTGGCTTCGCAGATGGCCGGAGCGCGCTTTCACGACACCACTGTCACCATTGCCTGTGCTCAC
AGCCAGTGGCGCGCCAAGGGCGAACGTCTGCTTTTCCCCGGTTTTATGGCGGCCCTGCCCCGCGCCGGAGAAGACGCCGA
TTCCGATCTGCCGCCTGTGGAGGTCGGGCAGACCCTCAAGCTGGAAAAGATGGACAAGGAACAAAAGTTCACCCAGCCTC
CGCCGCGCTACAGCGAGGCGAGCCTCGTGCGCGAGCTTGAAGAGCGCGGCATCGGGCGTCCTTCCACCTATGCGGCCATT
ATATCCACATTGCAGGACAGGGAATATGTGAGCCTGGCCGAAAGGCACTTTGTGCCTACAGATCTCGGGCGTGTGGTTTG
CGGCCAACTGACCGAACATTTTTCGCGGTTGATGGACGTGGGCTTTACCGCCCAGATGGAAGAAGGGCTGGACAAGGTGG
CCGAGGGCAAGGAGCACTGGGTAGAGCTTTTGCGTGCCTTTGCCGACGATTTCAATCCTACCCTTACGGCCGCTGCCAAA
AACATGCAGAGCGTCAAGGGCGGCATCCCCGCCGATCTAGCCTGCCCGGAATGCGGCAAGCCGCTCATGATCAAGTTCGG
CAAGGCCGGCGCTTTTCTGGCCTGCTCCGGCTACCCCGACTGCAATTTCACCAGCAACTTCTCCCGTAACGAGGAGGGCA
AGGTGGAGGCCGTGGCCGCTGAAAAGCCGAAGTATGAAAAGGTGGGGCAGTGCCCCAAGTGCGGGCGCGACCTTGTCATC
AAAAAAGCCCGCACAGGCAGCAGCTTTATCGCCTGCACGGGCTATCCCGAATGCAGGCATGCGGCCCCCCTGTCCACGGG
CGTGCCCTGTCCGCGCTGCGAAAAGGGGCAGCTGGTGGAAAAAAGCACCAAGCGTGGCAAGATATTCTATTCTTGCGACC
AGTACCCGCAGTGCGACTTTGCCCTGTGGGACAGGCCCGTACCCGGCCCCTGCCCGCGCTGTAACTCCCCTTATCTGGTG
GAAAAGAAGAGCCGGGACGGCCTGAAGGTCATATGCCCGGTCAAGGGCTGCGGCTATGTGAAGGAGGAAGATCATGAGCA
GGAGTGA

Upstream 100 bases:

>100_bases
CGGCCCGCACTTGACGCCTTCGGGTTTTATCGTATCTTGCCGTTACATAAAGGGCGCGCAGGACATTTTTGGCGCAGCCG
GAAAGCCAATTGAGGGCAGC

Downstream 100 bases:

>100_bases
CGAACCTGGCGGCGGGGCCGTCAGTACCGGCCCTGCGGGGGCCTGTTCCGCTGACGCTGCCGGTTCCGGCGGCATGAGCG
CCCCCTGGCCCGACACGCTG

Product: DNA topoisomerase I

Products: NA

Alternate protein names: DNA topoisomerase I; Omega-protein; Relaxing enzyme; Swivelase; Untwisting enzyme [H]

Number of amino acids: Translated: 748; Mature: 747

Protein sequence:

>748_residues
MGKQLIIVESPAKVKTIKKFLGPAYMVQASVGHVRDLPAKTLGVDEEHDFAPHYEVIENKKNVVSDLRAAASKAETVYLA
PDPDREGEAIAWHVAELIRDKAKDIKRIQFNEITAKAVKEALAHPRELNADLFDAQQARRILDRLVGYKISPLLWKTIKR
GISAGRVQSVALRLIVEREEEREVFRPEEYWLFKALLSGEVPPPFKADLLKVNGKKAVIGNAEEAQALEAALKGQPFVVE
NVEQKERERAPQPPFITSTLQQAANQRLSYSAKRTMNIAQRLYEGVELGDRGLTALITYMRTDSTRIADEARDAAKKFIG
EHFGKDYLPKRARVYKTKGSAQDAHEAIRPVDVSITPDEVKQHLPPDQYNLYRLIWSRFVASQMAGARFHDTTVTIACAH
SQWRAKGERLLFPGFMAALPRAGEDADSDLPPVEVGQTLKLEKMDKEQKFTQPPPRYSEASLVRELEERGIGRPSTYAAI
ISTLQDREYVSLAERHFVPTDLGRVVCGQLTEHFSRLMDVGFTAQMEEGLDKVAEGKEHWVELLRAFADDFNPTLTAAAK
NMQSVKGGIPADLACPECGKPLMIKFGKAGAFLACSGYPDCNFTSNFSRNEEGKVEAVAAEKPKYEKVGQCPKCGRDLVI
KKARTGSSFIACTGYPECRHAAPLSTGVPCPRCEKGQLVEKSTKRGKIFYSCDQYPQCDFALWDRPVPGPCPRCNSPYLV
EKKSRDGLKVICPVKGCGYVKEEDHEQE

Sequences:

>Translated_748_residues
MGKQLIIVESPAKVKTIKKFLGPAYMVQASVGHVRDLPAKTLGVDEEHDFAPHYEVIENKKNVVSDLRAAASKAETVYLA
PDPDREGEAIAWHVAELIRDKAKDIKRIQFNEITAKAVKEALAHPRELNADLFDAQQARRILDRLVGYKISPLLWKTIKR
GISAGRVQSVALRLIVEREEEREVFRPEEYWLFKALLSGEVPPPFKADLLKVNGKKAVIGNAEEAQALEAALKGQPFVVE
NVEQKERERAPQPPFITSTLQQAANQRLSYSAKRTMNIAQRLYEGVELGDRGLTALITYMRTDSTRIADEARDAAKKFIG
EHFGKDYLPKRARVYKTKGSAQDAHEAIRPVDVSITPDEVKQHLPPDQYNLYRLIWSRFVASQMAGARFHDTTVTIACAH
SQWRAKGERLLFPGFMAALPRAGEDADSDLPPVEVGQTLKLEKMDKEQKFTQPPPRYSEASLVRELEERGIGRPSTYAAI
ISTLQDREYVSLAERHFVPTDLGRVVCGQLTEHFSRLMDVGFTAQMEEGLDKVAEGKEHWVELLRAFADDFNPTLTAAAK
NMQSVKGGIPADLACPECGKPLMIKFGKAGAFLACSGYPDCNFTSNFSRNEEGKVEAVAAEKPKYEKVGQCPKCGRDLVI
KKARTGSSFIACTGYPECRHAAPLSTGVPCPRCEKGQLVEKSTKRGKIFYSCDQYPQCDFALWDRPVPGPCPRCNSPYLV
EKKSRDGLKVICPVKGCGYVKEEDHEQE
>Mature_747_residues
GKQLIIVESPAKVKTIKKFLGPAYMVQASVGHVRDLPAKTLGVDEEHDFAPHYEVIENKKNVVSDLRAAASKAETVYLAP
DPDREGEAIAWHVAELIRDKAKDIKRIQFNEITAKAVKEALAHPRELNADLFDAQQARRILDRLVGYKISPLLWKTIKRG
ISAGRVQSVALRLIVEREEEREVFRPEEYWLFKALLSGEVPPPFKADLLKVNGKKAVIGNAEEAQALEAALKGQPFVVEN
VEQKERERAPQPPFITSTLQQAANQRLSYSAKRTMNIAQRLYEGVELGDRGLTALITYMRTDSTRIADEARDAAKKFIGE
HFGKDYLPKRARVYKTKGSAQDAHEAIRPVDVSITPDEVKQHLPPDQYNLYRLIWSRFVASQMAGARFHDTTVTIACAHS
QWRAKGERLLFPGFMAALPRAGEDADSDLPPVEVGQTLKLEKMDKEQKFTQPPPRYSEASLVRELEERGIGRPSTYAAII
STLQDREYVSLAERHFVPTDLGRVVCGQLTEHFSRLMDVGFTAQMEEGLDKVAEGKEHWVELLRAFADDFNPTLTAAAKN
MQSVKGGIPADLACPECGKPLMIKFGKAGAFLACSGYPDCNFTSNFSRNEEGKVEAVAAEKPKYEKVGQCPKCGRDLVIK
KARTGSSFIACTGYPECRHAAPLSTGVPCPRCEKGQLVEKSTKRGKIFYSCDQYPQCDFALWDRPVPGPCPRCNSPYLVE
KKSRDGLKVICPVKGCGYVKEEDHEQE

Specific function: The reaction catalyzed by topoisomerases leads to the conversion of one topological isomer of DNA to another [H]

COG id: COG0550

COG function: function code L; Topoisomerase IA

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 Toprim domain [H]

Homologues:

Organism=Homo sapiens, GI10835218, Length=588, Percent_Identity=24.3197278911565, Blast_Score=131, Evalue=2e-30,
Organism=Homo sapiens, GI4507635, Length=695, Percent_Identity=22.7338129496403, Blast_Score=127, Evalue=5e-29,
Organism=Escherichia coli, GI1787529, Length=702, Percent_Identity=42.4501424501425, Blast_Score=523, Evalue=1e-149,
Organism=Escherichia coli, GI48994931, Length=170, Percent_Identity=41.7647058823529, Blast_Score=142, Evalue=8e-35,
Organism=Escherichia coli, GI1788061, Length=570, Percent_Identity=25.4385964912281, Blast_Score=112, Evalue=1e-25,
Organism=Caenorhabditis elegans, GI32563869, Length=561, Percent_Identity=25.3119429590018, Blast_Score=139, Evalue=5e-33,
Organism=Caenorhabditis elegans, GI17555378, Length=528, Percent_Identity=22.5378787878788, Blast_Score=96, Evalue=9e-20,
Organism=Drosophila melanogaster, GI24640096, Length=528, Percent_Identity=22.7272727272727, Blast_Score=108, Evalue=2e-23,
Organism=Drosophila melanogaster, GI24585251, Length=570, Percent_Identity=23.5087719298246, Blast_Score=107, Evalue=2e-23,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003601
- InterPro:   IPR013497
- InterPro:   IPR013824
- InterPro:   IPR013825
- InterPro:   IPR000380
- InterPro:   IPR003602
- InterPro:   IPR013498
- InterPro:   IPR005733
- InterPro:   IPR006171 [H]

Pfam domain/function: PF01131 Topoisom_bac; PF01751 Toprim; PF01396 zf-C4_Topoisom [H]

EC number: =5.99.1.2 [H]

Molecular weight: Translated: 83473; Mature: 83342

Theoretical pI: Translated: 8.25; Mature: 8.25

Prosite motif: PS00396 TOPOISOMERASE_I_PROK

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.4 %Cys     (Translated Protein)
1.5 %Met     (Translated Protein)
3.9 %Cys+Met (Translated Protein)
2.4 %Cys     (Mature Protein)
1.3 %Met     (Mature Protein)
3.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MGKQLIIVESPAKVKTIKKFLGPAYMVQASVGHVRDLPAKTLGVDEEHDFAPHYEVIENK
CCCEEEEEECCHHHHHHHHHHCCHHEEHHHHHHHHCCCHHHCCCCCCCCCCCCHHHHHHH
KNVVSDLRAAASKAETVYLAPDPDREGEAIAWHVAELIRDKAKDIKRIQFNEITAKAVKE
HHHHHHHHHHHCCCCEEEECCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
ALAHPRELNADLFDAQQARRILDRLVGYKISPLLWKTIKRGISAGRVQSVALRLIVEREE
HHCCCHHCCCHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHCCCCHHHHHHHHHHHHHCHH
EREVFRPEEYWLFKALLSGEVPPPFKADLLKVNGKKAVIGNAEEAQALEAALKGQPFVVE
HHHHCCCHHHHHHHHHHCCCCCCCCCCCCEEECCCEEEECCCHHHHHHHHHHCCCCEEEC
NVEQKERERAPQPPFITSTLQQAANQRLSYSAKRTMNIAQRLYEGVELGDRGLTALITYM
CHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHH
RTDSTRIADEARDAAKKFIGEHFGKDYLPKRARVYKTKGSAQDAHEAIRPVDVSITPDEV
HCCCHHHHHHHHHHHHHHHHHHCCCCCCCCHHHEEECCCCHHHHHHHHCCCCEEECHHHH
KQHLPPDQYNLYRLIWSRFVASQMAGARFHDTTVTIACAHSQWRAKGERLLFPGFMAALP
HHCCCCCHHHHHHHHHHHHHHHHHCCCEEECCEEEEEEECHHHHCCCCEEECCHHHHHCC
RAGEDADSDLPPVEVGQTLKLEKMDKEQKFTQPPPRYSEASLVRELEERGIGRPSTYAAI
CCCCCCCCCCCCHHCCCCEEHHHHCHHHHCCCCCCCCHHHHHHHHHHHCCCCCCHHHHHH
ISTLQDREYVSLAERHFVPTDLGRVVCGQLTEHFSRLMDVGFTAQMEEGLDKVAEGKEHW
HHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHCCHHHH
VELLRAFADDFNPTLTAAAKNMQSVKGGIPADLACPECGKPLMIKFGKAGAFLACSGYPD
HHHHHHHHHCCCCHHHHHHHHHHHHCCCCCCCCCCCCCCCCEEEEECCCCCEEEECCCCC
CNFTSNFSRNEEGKVEAVAAEKPKYEKVGQCPKCGRDLVIKKARTGSSFIACTGYPECRH
CCCCCCCCCCCCCCEEEEECCCCCHHHHCCCCCCCCHHEEEECCCCCCEEEECCCCCHHC
AAPLSTGVPCPRCEKGQLVEKSTKRGKIFYSCDQYPQCDFALWDRPVPGPCPRCNSPYLV
CCCCCCCCCCCCCCCCCHHHHHCCCCEEEEECCCCCCCCHHHCCCCCCCCCCCCCCCCEE
EKKSRDGLKVICPVKGCGYVKEEDHEQE
EECCCCCCEEEECCCCCCCCCCCCCCCC
>Mature Secondary Structure 
GKQLIIVESPAKVKTIKKFLGPAYMVQASVGHVRDLPAKTLGVDEEHDFAPHYEVIENK
CCEEEEEECCHHHHHHHHHHCCHHEEHHHHHHHHCCCHHHCCCCCCCCCCCCHHHHHHH
KNVVSDLRAAASKAETVYLAPDPDREGEAIAWHVAELIRDKAKDIKRIQFNEITAKAVKE
HHHHHHHHHHHCCCCEEEECCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
ALAHPRELNADLFDAQQARRILDRLVGYKISPLLWKTIKRGISAGRVQSVALRLIVEREE
HHCCCHHCCCHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHCCCCHHHHHHHHHHHHHCHH
EREVFRPEEYWLFKALLSGEVPPPFKADLLKVNGKKAVIGNAEEAQALEAALKGQPFVVE
HHHHCCCHHHHHHHHHHCCCCCCCCCCCCEEECCCEEEECCCHHHHHHHHHHCCCCEEEC
NVEQKERERAPQPPFITSTLQQAANQRLSYSAKRTMNIAQRLYEGVELGDRGLTALITYM
CHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHH
RTDSTRIADEARDAAKKFIGEHFGKDYLPKRARVYKTKGSAQDAHEAIRPVDVSITPDEV
HCCCHHHHHHHHHHHHHHHHHHCCCCCCCCHHHEEECCCCHHHHHHHHCCCCEEECHHHH
KQHLPPDQYNLYRLIWSRFVASQMAGARFHDTTVTIACAHSQWRAKGERLLFPGFMAALP
HHCCCCCHHHHHHHHHHHHHHHHHCCCEEECCEEEEEEECHHHHCCCCEEECCHHHHHCC
RAGEDADSDLPPVEVGQTLKLEKMDKEQKFTQPPPRYSEASLVRELEERGIGRPSTYAAI
CCCCCCCCCCCCHHCCCCEEHHHHCHHHHCCCCCCCCHHHHHHHHHHHCCCCCCHHHHHH
ISTLQDREYVSLAERHFVPTDLGRVVCGQLTEHFSRLMDVGFTAQMEEGLDKVAEGKEHW
HHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHCCHHHH
VELLRAFADDFNPTLTAAAKNMQSVKGGIPADLACPECGKPLMIKFGKAGAFLACSGYPD
HHHHHHHHHCCCCHHHHHHHHHHHHCCCCCCCCCCCCCCCCEEEEECCCCCEEEECCCCC
CNFTSNFSRNEEGKVEAVAAEKPKYEKVGQCPKCGRDLVIKKARTGSSFIACTGYPECRH
CCCCCCCCCCCCCCEEEEECCCCCHHHHCCCCCCCCHHEEEECCCCCCEEEECCCCCHHC
AAPLSTGVPCPRCEKGQLVEKSTKRGKIFYSCDQYPQCDFALWDRPVPGPCPRCNSPYLV
CCCCCCCCCCCCCCCCCHHHHHCCCCEEEEECCCCCCCCHHHCCCCCCCCCCCCCCCCEE
EKKSRDGLKVICPVKGCGYVKEEDHEQE
EECCCCCCEEEECCCCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 11058132 [H]