Click here to switch to the map view.

The map label for this gene is ackA

Identifier: 220903577

GI number: 220903577

Start: 355524

End: 356735

Strand: Reverse

Name: ackA

Synonym: Ddes_0296

Alternate gene names: 220903577

Gene position: 356735-355524 (Counterclockwise)

Preceding gene: 220903578

Following gene: 220903576

Centisome position: 12.41

GC content: 59.16

Gene sequence:

>1212_bases
ATGAAAATCCTGGTCATCAACGCGGGCTCTTCGTCCTGCAAGTACCAACTGCTGGAAATGGACACCCACGCCGTTCTCTG
CTCCGGCCTTGCCGAACGCATCGGACAGGACGAAGGCCGTCTGACCCACAAGATCGCCCCTGATACGGACAAAGAAGAAA
AAATCGTCCAGACCGCGCACTTTCCCACCCATGTGCAGGCGATGGAAATGGTCATCGCCCTGCTCACCGATGCTGAAAAA
GGCGTCATCAAGGACAAGAGCGAAATTGCGGGCATCGGCCACCGCGTGCTGCACGGCGGCGAAGCCGTCAGCGATCCCGT
GCTGGTGGACGAACGCGTGAAGGGCATTGTGCGCGAATGCGCCGTGCTTGGCCCCCTGCACAACCCCGCAAACCTTATGG
GCATTGAAGTGGCTGAAAAGCTCTTCCCCGGCGTGCCCAACGTGGCTGTGTTTGATACGGAATTCGGCATGGGCATGCCC
AAAGAAGCTTTCATGTACGCCCTGCCCTACGAGTTGTATGAAGACCTGCGCATCCGCCGCTACGGCTTCCACGGCACGTC
GCACAAATACATCGCGGGCGCTACTGCCAAGTACCTCGGCAAGCCCCTGAGCGAGCTGCGCTCCATCACCATGCACCTTG
GCAACGGCTCATCCATGAGCTGCGTGCGCAACGGCAAGTGCTTTGACACCAGCATGGGGCTGACCCCTCTTGAAGGCCTC
ATCATGGGGACGCGCTGCGGCAGCATCGACCCCGCCATTGTGCCTTTTGTCATGGAAAAGAAGGGCCTCACCCCCGAGCA
GGTGGATACCCTGATGAACAAGAAGTCCGGGTTGCTCGGCCTGTGCGGCCACACCGATATGCGCGACGTGCATGCCGAAG
TGGAAAAAGGCAACGAGCGTGCCGCCCTGGCCCTCAAGATGCTGGTCCGCAGCATCAAGAAAACCCTTGGCTCTTACTAC
TTCCTGCTGGACGGCAAGGTGGATGCCCTGGTCTTTACCGCCGGCATCGGCGAAAATGACGACATCGTGCGTGCCGAAGT
GTGCGCCGGGCTTGAAAACCTCGGCATCAAAATCGACGCCAAGGAAAACGGCACCCGCAAGGCCGGTGCACGTGCCATCT
CCACACCTGACAGCTCCATACCCGTGCTGATCATTCCCACCAATGAAGAACTGCAGATCGCCATGGCCACGGTTGAAGTG
CTGGGCAAATAA

Upstream 100 bases:

>100_bases
AAGCCGCCACGGCTGACGCCCGCAGGCCGAAGCCGCAAAACTGCTATCCAGGGGAGGGGACGGCCCTCCCCACATTTCGT
TAACAAGGAAAGGAAGACAC

Downstream 100 bases:

>100_bases
GCCTGCCGCACCGTAGCCGGCATCCGCGCCGGAATATGCAGAAGGCCGCTTCCCACGAAGCGGCCTTCTGTGTGCAGGGC
GTTACAAAAAACTGTTCAGG

Product: acetate kinase

Products: NA

Alternate protein names: Acetokinase

Number of amino acids: Translated: 403; Mature: 403

Protein sequence:

>403_residues
MKILVINAGSSSCKYQLLEMDTHAVLCSGLAERIGQDEGRLTHKIAPDTDKEEKIVQTAHFPTHVQAMEMVIALLTDAEK
GVIKDKSEIAGIGHRVLHGGEAVSDPVLVDERVKGIVRECAVLGPLHNPANLMGIEVAEKLFPGVPNVAVFDTEFGMGMP
KEAFMYALPYELYEDLRIRRYGFHGTSHKYIAGATAKYLGKPLSELRSITMHLGNGSSMSCVRNGKCFDTSMGLTPLEGL
IMGTRCGSIDPAIVPFVMEKKGLTPEQVDTLMNKKSGLLGLCGHTDMRDVHAEVEKGNERAALALKMLVRSIKKTLGSYY
FLLDGKVDALVFTAGIGENDDIVRAEVCAGLENLGIKIDAKENGTRKAGARAISTPDSSIPVLIIPTNEELQIAMATVEV
LGK

Sequences:

>Translated_403_residues
MKILVINAGSSSCKYQLLEMDTHAVLCSGLAERIGQDEGRLTHKIAPDTDKEEKIVQTAHFPTHVQAMEMVIALLTDAEK
GVIKDKSEIAGIGHRVLHGGEAVSDPVLVDERVKGIVRECAVLGPLHNPANLMGIEVAEKLFPGVPNVAVFDTEFGMGMP
KEAFMYALPYELYEDLRIRRYGFHGTSHKYIAGATAKYLGKPLSELRSITMHLGNGSSMSCVRNGKCFDTSMGLTPLEGL
IMGTRCGSIDPAIVPFVMEKKGLTPEQVDTLMNKKSGLLGLCGHTDMRDVHAEVEKGNERAALALKMLVRSIKKTLGSYY
FLLDGKVDALVFTAGIGENDDIVRAEVCAGLENLGIKIDAKENGTRKAGARAISTPDSSIPVLIIPTNEELQIAMATVEV
LGK
>Mature_403_residues
MKILVINAGSSSCKYQLLEMDTHAVLCSGLAERIGQDEGRLTHKIAPDTDKEEKIVQTAHFPTHVQAMEMVIALLTDAEK
GVIKDKSEIAGIGHRVLHGGEAVSDPVLVDERVKGIVRECAVLGPLHNPANLMGIEVAEKLFPGVPNVAVFDTEFGMGMP
KEAFMYALPYELYEDLRIRRYGFHGTSHKYIAGATAKYLGKPLSELRSITMHLGNGSSMSCVRNGKCFDTSMGLTPLEGL
IMGTRCGSIDPAIVPFVMEKKGLTPEQVDTLMNKKSGLLGLCGHTDMRDVHAEVEKGNERAALALKMLVRSIKKTLGSYY
FLLDGKVDALVFTAGIGENDDIVRAEVCAGLENLGIKIDAKENGTRKAGARAISTPDSSIPVLIIPTNEELQIAMATVEV
LGK

Specific function: Involved In The Activation Of Acetate To Acetyl CoA And The Secretion Of Acetate. During Anaerobic Growth Of The Organism, This Enzyme Is Also Involved In The Synthesis Of Most Of The ATP Formed Catabolically. [C]

COG id: COG0282

COG function: function code C; Acetate kinase

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the acetokinase family

Homologues:

Organism=Escherichia coli, GI1788633, Length=395, Percent_Identity=43.5443037974684, Blast_Score=306, Evalue=2e-84,
Organism=Escherichia coli, GI145693184, Length=403, Percent_Identity=38.9578163771712, Blast_Score=273, Evalue=1e-74,

Paralogues:

None

Copy number: 1,800 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): ACKA_DESDA (B8J374)

Other databases:

- EMBL:   CP001358
- RefSeq:   YP_002478889.1
- GeneID:   7283952
- GenomeReviews:   CP001358_GR
- KEGG:   dds:Ddes_0296
- HOGENOM:   HBG285863
- ProtClustDB:   PRK00180
- GO:   GO:0005737
- HAMAP:   MF_00020
- InterPro:   IPR000890
- InterPro:   IPR004372
- PANTHER:   PTHR21060
- PANTHER:   PTHR21060:SF11
- PIRSF:   PIRSF000722
- PRINTS:   PR00471
- TIGRFAMs:   TIGR00016

Pfam domain/function: PF00871 Acetate_kinase

EC number: =2.7.2.1

Molecular weight: Translated: 43545; Mature: 43545

Theoretical pI: Translated: 6.38; Mature: 6.38

Prosite motif: PS01075 ACETATE_KINASE_1; PS01076 ACETATE_KINASE_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.0 %Cys     (Translated Protein)
4.2 %Met     (Translated Protein)
6.2 %Cys+Met (Translated Protein)
2.0 %Cys     (Mature Protein)
4.2 %Met     (Mature Protein)
6.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKILVINAGSSSCKYQLLEMDTHAVLCSGLAERIGQDEGRLTHKIAPDTDKEEKIVQTAH
CEEEEEECCCCCCEEEEEECCHHHHHHHHHHHHHCCCCCCEEEEECCCCCHHHHHHHHCC
FPTHVQAMEMVIALLTDAEKGVIKDKSEIAGIGHRVLHGGEAVSDPVLVDERVKGIVREC
CCHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHHH
AVLGPLHNPANLMGIEVAEKLFPGVPNVAVFDTEFGMGMPKEAFMYALPYELYEDLRIRR
HHHCCCCCCCHHHHHHHHHHHCCCCCCEEEEECCCCCCCCHHHHHHHCCHHHHHHHHHEE
YGFHGTSHKYIAGATAKYLGKPLSELRSITMHLGNGSSMSCVRNGKCFDTSMGLTPLEGL
ECCCCCCCCEEECCHHHHHCCHHHHHHHHHEECCCCCCEEEECCCCEEECCCCCCCHHHE
IMGTRCGSIDPAIVPFVMEKKGLTPEQVDTLMNKKSGLLGLCGHTDMRDVHAEVEKGNER
EEECCCCCCCCHHHHHHHHCCCCCHHHHHHHHHCCCCEEEECCCCCHHHHHHHHHCCCCH
AALALKMLVRSIKKTLGSYYFLLDGKVDALVFTAGIGENDDIVRAEVCAGLENLGIKIDA
HHHHHHHHHHHHHHHHCCEEEEECCCEEEEEEEECCCCCCCEEHHHHHCCHHHCCEEEEC
KENGTRKAGARAISTPDSSIPVLIIPTNEELQIAMATVEVLGK
CCCCCHHHCCEEECCCCCCCCEEEEECCCCEEEHEEHHHHHCC
>Mature Secondary Structure
MKILVINAGSSSCKYQLLEMDTHAVLCSGLAERIGQDEGRLTHKIAPDTDKEEKIVQTAH
CEEEEEECCCCCCEEEEEECCHHHHHHHHHHHHHCCCCCCEEEEECCCCCHHHHHHHHCC
FPTHVQAMEMVIALLTDAEKGVIKDKSEIAGIGHRVLHGGEAVSDPVLVDERVKGIVREC
CCHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHHH
AVLGPLHNPANLMGIEVAEKLFPGVPNVAVFDTEFGMGMPKEAFMYALPYELYEDLRIRR
HHHCCCCCCCHHHHHHHHHHHCCCCCCEEEEECCCCCCCCHHHHHHHCCHHHHHHHHHEE
YGFHGTSHKYIAGATAKYLGKPLSELRSITMHLGNGSSMSCVRNGKCFDTSMGLTPLEGL
ECCCCCCCCEEECCHHHHHCCHHHHHHHHHEECCCCCCEEEECCCCEEECCCCCCCHHHE
IMGTRCGSIDPAIVPFVMEKKGLTPEQVDTLMNKKSGLLGLCGHTDMRDVHAEVEKGNER
EEECCCCCCCCHHHHHHHHCCCCCHHHHHHHHHCCCCEEEECCCCCHHHHHHHHHCCCCH
AALALKMLVRSIKKTLGSYYFLLDGKVDALVFTAGIGENDDIVRAEVCAGLENLGIKIDA
HHHHHHHHHHHHHHHHCCEEEEECCCEEEEEEEECCCCCCCEEHHHHHCCHHHCCEEEEC
KENGTRKAGARAISTPDSSIPVLIIPTNEELQIAMATVEVLGK
CCCCCHHHCCEEECCCCCCCCEEEEECCCCEEEHEEHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA