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The map label for this gene is recA

Identifier: 220903572

GI number: 220903572

Start: 348360

End: 349439

Strand: Reverse

Name: recA

Synonym: Ddes_0291

Alternate gene names: 220903572

Gene position: 349439-348360 (Counterclockwise)

Preceding gene: 220903576

Following gene: 220903571

Centisome position: 12.16

GC content: 61.39

Gene sequence:

>1080_bases
ATGGCCAGAAAACCGGCCCTCAGCCCTGAAGAAGCACGCGCTGAAGCCCTGGGCACAGCCCTTGCCACTATTGAACGCAA
ATACGGCAAGGGCGCCGTCATGAAACTTTCTGACGATGCCCATGTGAATATACCTGTTATCCCCACCGGTTCCATCGGCC
TCGACCTGGCCCTTGGCGTGGGGGGCATTCCGCGCGGGCGCATATCTGAAATTTTTGGTCCCGAATCATCGGGCAAAACC
ACCCTCACCCTGCACATCATTGCCGAATGCCAGCGTCTGGGCGGCACCTGCGCCTTTGTGGACGCCGAGCACGCCCTGGA
CGTGTCTTACGCCAGACGGCTGGGCGTCAACACCGATGAACTGCTGATCTCGCAGCCCGACTACGGCGAGCAGGCCCTGG
ACATCGCGGACATGCTGGTCCGCTCCGGCGCTGTGGACCTTGTGGTGGTGGACTCTGTGGCGGCACTCATTCCCCAGGCC
GAACTGGAAGGCGACATGGGCGAAACCCAGGTGGGCGGCCACGCCCGCCTCATGTCGCACGCCATGCGCCGCCTCACGGG
CACTATCCACAAGTCGCGCACTTCGGTCATCTTTATCAACCAGATACGCATGAAGATCGGCGTCACGGGCTACGGCAGCC
CCGAAACCACCACGGGCGGCAACGCGCTCAAGTTCTACTCTTCCGTGCGCCTGGACATCCGCCGCATACAGACCCTCAAG
GACAAGGAAGAATCCTTCGGCTCGCGCACCAGGGTCAAGGTGGTGAAAAACAAGGTCGCGCCGCCCTTCCGCAGCGCGGT
TTTCGACATTCTGTACGGCCTGGGCATCTCACGCGCGGGCGAACTGCTTGACCTCGGCATCGAGGCCAAAATCATCGACC
AGAGCGGCTCATGGTTCGCCTTCGGCGCAGAAAAGCTGGGCCAGGGCCGCGAAAAGGTGCGTGCCCTGCTGGACGAAAAC
ATCGACCTGCGCAATCAGATCGAAGCCAAGGTTGTGGAATTTCTGGGCCTTCACCCCCGGGAATTCACCCCCACCGCCGA
AGATATTGCGGAAAGCCAAGACCCCGTTCTCGAAGACTGA

Upstream 100 bases:

>100_bases
GTCCACAGGGGTAGGGACCGAGGCCCCGCGCATTTGCCTTGCCCCACGCCCTTTGCTATAATGATGCGCTTGAAGATATC
TTTACCCGGAGCATAAAATC

Downstream 100 bases:

>100_bases
CGTTTTCCGGCAGGCCCGGCAGCCGCTGCGTAAGCATGCGGCTGCCGGGCCTGTGCCGTAAACAGTTATCGCAATGACCA
GAAAGGCCGCTGCCTGAAGG

Product: recombinase A

Products: NA

Alternate protein names: Recombinase A

Number of amino acids: Translated: 359; Mature: 358

Protein sequence:

>359_residues
MARKPALSPEEARAEALGTALATIERKYGKGAVMKLSDDAHVNIPVIPTGSIGLDLALGVGGIPRGRISEIFGPESSGKT
TLTLHIIAECQRLGGTCAFVDAEHALDVSYARRLGVNTDELLISQPDYGEQALDIADMLVRSGAVDLVVVDSVAALIPQA
ELEGDMGETQVGGHARLMSHAMRRLTGTIHKSRTSVIFINQIRMKIGVTGYGSPETTTGGNALKFYSSVRLDIRRIQTLK
DKEESFGSRTRVKVVKNKVAPPFRSAVFDILYGLGISRAGELLDLGIEAKIIDQSGSWFAFGAEKLGQGREKVRALLDEN
IDLRNQIEAKVVEFLGLHPREFTPTAEDIAESQDPVLED

Sequences:

>Translated_359_residues
MARKPALSPEEARAEALGTALATIERKYGKGAVMKLSDDAHVNIPVIPTGSIGLDLALGVGGIPRGRISEIFGPESSGKT
TLTLHIIAECQRLGGTCAFVDAEHALDVSYARRLGVNTDELLISQPDYGEQALDIADMLVRSGAVDLVVVDSVAALIPQA
ELEGDMGETQVGGHARLMSHAMRRLTGTIHKSRTSVIFINQIRMKIGVTGYGSPETTTGGNALKFYSSVRLDIRRIQTLK
DKEESFGSRTRVKVVKNKVAPPFRSAVFDILYGLGISRAGELLDLGIEAKIIDQSGSWFAFGAEKLGQGREKVRALLDEN
IDLRNQIEAKVVEFLGLHPREFTPTAEDIAESQDPVLED
>Mature_358_residues
ARKPALSPEEARAEALGTALATIERKYGKGAVMKLSDDAHVNIPVIPTGSIGLDLALGVGGIPRGRISEIFGPESSGKTT
LTLHIIAECQRLGGTCAFVDAEHALDVSYARRLGVNTDELLISQPDYGEQALDIADMLVRSGAVDLVVVDSVAALIPQAE
LEGDMGETQVGGHARLMSHAMRRLTGTIHKSRTSVIFINQIRMKIGVTGYGSPETTTGGNALKFYSSVRLDIRRIQTLKD
KEESFGSRTRVKVVKNKVAPPFRSAVFDILYGLGISRAGELLDLGIEAKIIDQSGSWFAFGAEKLGQGREKVRALLDENI
DLRNQIEAKVVEFLGLHPREFTPTAEDIAESQDPVLED

Specific function: Can catalyze the hydrolysis of ATP in the presence of single-stranded DNA, the ATP-dependent uptake of single-stranded DNA by duplex DNA, and the ATP-dependent hybridization of homologous single-stranded DNAs. It interacts with lexA causing its activation

COG id: COG0468

COG function: function code L; RecA/RadA recombinase

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the recA family

Homologues:

Organism=Escherichia coli, GI1789051, Length=353, Percent_Identity=56.657223796034, Blast_Score=405, Evalue=1e-114,

Paralogues:

None

Copy number: 1548 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. 800-1200 (L-broth) 40,000-60,000 (L-broth + Nalidixate) 4,000 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): RECA_DESDA (B8J369)

Other databases:

- EMBL:   CP001358
- RefSeq:   YP_002478884.1
- GeneID:   7283947
- GenomeReviews:   CP001358_GR
- KEGG:   dds:Ddes_0291
- HOGENOM:   HBG339889
- ProtClustDB:   PRK09354
- GO:   GO:0005737
- HAMAP:   MF_00268
- InterPro:   IPR003593
- InterPro:   IPR013765
- InterPro:   IPR020584
- InterPro:   IPR020588
- InterPro:   IPR020587
- PANTHER:   PTHR22942:SF1
- PRINTS:   PR00142
- SMART:   SM00382
- TIGRFAMs:   TIGR02012

Pfam domain/function: PF00154 RecA

EC number: NA

Molecular weight: Translated: 38759; Mature: 38628

Theoretical pI: Translated: 5.71; Mature: 5.71

Prosite motif: PS00321 RECA_1; PS50162 RECA_2; PS50163 RECA_3

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
1.9 %Met     (Translated Protein)
2.5 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
1.7 %Met     (Mature Protein)
2.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MARKPALSPEEARAEALGTALATIERKYGKGAVMKLSDDAHVNIPVIPTGSIGLDLALGV
CCCCCCCCHHHHHHHHHHHHHHHHHHHHCCCEEEEECCCCEEEEEEEECCCCCEEEEECC
GGIPRGRISEIFGPESSGKTTLTLHIIAECQRLGGTCAFVDAEHALDVSYARRLGVNTDE
CCCCCCHHHHHCCCCCCCCEEEEEEEEHHHHHHCCEEEEECCHHHHHHHHHHHHCCCCHH
LLISQPDYGEQALDIADMLVRSGAVDLVVVDSVAALIPQAELEGDMGETQVGGHARLMSH
EEEECCCCCHHHHHHHHHHHHCCCCEEEEHHHHHHHCCHHHHCCCCCCCCCCCHHHHHHH
AMRRLTGTIHKSRTSVIFINQIRMKIGVTGYGSPETTTGGNALKFYSSVRLDIRRIQTLK
HHHHHHHHHHCCCCCEEEEEEEEEEEEECCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHH
DKEESFGSRTRVKVVKNKVAPPFRSAVFDILYGLGISRAGELLDLGIEAKIIDQSGSWFA
HHHHHCCCHHHHHHHHHHCCCHHHHHHHHHHHHCCCCCCCHHHCCCCEEEEEECCCCEEE
FGAEKLGQGREKVRALLDENIDLRNQIEAKVVEFLGLHPREFTPTAEDIAESQDPVLED
ECHHHHCCHHHHHHHHHHCCCCHHHHHHHHHHHHHCCCCCCCCCCHHHHHCCCCCCCCC
>Mature Secondary Structure 
ARKPALSPEEARAEALGTALATIERKYGKGAVMKLSDDAHVNIPVIPTGSIGLDLALGV
CCCCCCCHHHHHHHHHHHHHHHHHHHHCCCEEEEECCCCEEEEEEEECCCCCEEEEECC
GGIPRGRISEIFGPESSGKTTLTLHIIAECQRLGGTCAFVDAEHALDVSYARRLGVNTDE
CCCCCCHHHHHCCCCCCCCEEEEEEEEHHHHHHCCEEEEECCHHHHHHHHHHHHCCCCHH
LLISQPDYGEQALDIADMLVRSGAVDLVVVDSVAALIPQAELEGDMGETQVGGHARLMSH
EEEECCCCCHHHHHHHHHHHHCCCCEEEEHHHHHHHCCHHHHCCCCCCCCCCCHHHHHHH
AMRRLTGTIHKSRTSVIFINQIRMKIGVTGYGSPETTTGGNALKFYSSVRLDIRRIQTLK
HHHHHHHHHHCCCCCEEEEEEEEEEEEECCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHH
DKEESFGSRTRVKVVKNKVAPPFRSAVFDILYGLGISRAGELLDLGIEAKIIDQSGSWFA
HHHHHCCCHHHHHHHHHHCCCHHHHHHHHHHHHCCCCCCCHHHCCCCEEEEEECCCCEEE
FGAEKLGQGREKVRALLDENIDLRNQIEAKVVEFLGLHPREFTPTAEDIAESQDPVLED
ECHHHHCCHHHHHHHHHHCCCCHHHHHHHHHHHHHCCCCCCCCCCHHHHHCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA