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The map label for this gene is vsr [H]

Identifier: 220903551

GI number: 220903551

Start: 313965

End: 314357

Strand: Direct

Name: vsr [H]

Synonym: Ddes_0270

Alternate gene names: 220903551

Gene position: 313965-314357 (Clockwise)

Preceding gene: 220903550

Following gene: 220903552

Centisome position: 10.93

GC content: 44.27

Gene sequence:

>393_bases
ATGACTAATCGCAGTGCAAATATGAAGGCTATTAAAAGCCGGAATACTAAACCGGAGTTAATGTTGCGGGCCTTGCTCAG
GCGATTGGGCTTCCCTGGCTACAGGCTTCACCGCAAAGATTTGCCAGGTAAGCCAGATGTTGCATACATTGGGCGTAAAA
AAGCCATATTTGTTCACGGCTGTTTTTGGCATGGGCACCCGTGCAAAAACGGTTATCACCTTCCGCAAACAAATCCAGGA
TATTGGGGGCCGAAAATCGAGCGCAATATGACCCGGGACAAAGAGCATATTGCAAAGCTTGAGGCGCTTGGCTGGTCAGT
TTTAATTGTTTGGGAATGTGAGCTGAATAAGGTAGATATTAAGATAATCGTTAAAAATTTCATGGAAAATTAA

Upstream 100 bases:

>100_bases
TAACGCCATTCAAAGACATTGCTTTTTGGCTAAAAACGGGGCAATTTGAGAAGGCTTTTATATCCCCCGCAACGACATTA
ACAAATTTTCCTACCCCAAA

Downstream 100 bases:

>100_bases
TTTACCCTGACGATTGCCTTACTCTCTTGATCTATTTACTATGCCCTTTGGATAATTTGGAGAAAAGTGTGCGAATTGTT
TCTTTATTCAGTGGAGCCGG

Product: DNA mismatch endonuclease Vsr

Products: DNA [C]

Alternate protein names: V.XorII [H]

Number of amino acids: Translated: 130; Mature: 129

Protein sequence:

>130_residues
MTNRSANMKAIKSRNTKPELMLRALLRRLGFPGYRLHRKDLPGKPDVAYIGRKKAIFVHGCFWHGHPCKNGYHLPQTNPG
YWGPKIERNMTRDKEHIAKLEALGWSVLIVWECELNKVDIKIIVKNFMEN

Sequences:

>Translated_130_residues
MTNRSANMKAIKSRNTKPELMLRALLRRLGFPGYRLHRKDLPGKPDVAYIGRKKAIFVHGCFWHGHPCKNGYHLPQTNPG
YWGPKIERNMTRDKEHIAKLEALGWSVLIVWECELNKVDIKIIVKNFMEN
>Mature_129_residues
TNRSANMKAIKSRNTKPELMLRALLRRLGFPGYRLHRKDLPGKPDVAYIGRKKAIFVHGCFWHGHPCKNGYHLPQTNPGY
WGPKIERNMTRDKEHIAKLEALGWSVLIVWECELNKVDIKIIVKNFMEN

Specific function: May nick XorII sequences that contain T:G mispairs resulting from m5C-deamination [H]

COG id: COG3727

COG function: function code L; DNA G:T-mismatch repair endonuclease

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the vsr family [H]

Homologues:

Organism=Escherichia coli, GI1788270, Length=114, Percent_Identity=43.859649122807, Blast_Score=101, Evalue=1e-23,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004603
- InterPro:   IPR011335 [H]

Pfam domain/function: PF03852 Vsr [H]

EC number: 3.1.-.- [C]

Molecular weight: Translated: 15150; Mature: 15019

Theoretical pI: Translated: 10.65; Mature: 10.65

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.3 %Cys     (Translated Protein)
3.8 %Met     (Translated Protein)
6.2 %Cys+Met (Translated Protein)
2.3 %Cys     (Mature Protein)
3.1 %Met     (Mature Protein)
5.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTNRSANMKAIKSRNTKPELMLRALLRRLGFPGYRLHRKDLPGKPDVAYIGRKKAIFVHG
CCCCCCCHHHHHCCCCCHHHHHHHHHHHCCCCCCEEECCCCCCCCCEEEECCCEEEEEEE
CFWHGHPCKNGYHLPQTNPGYWGPKIERNMTRDKEHIAKLEALGWSVLIVWECELNKVDI
EEECCCCCCCCEECCCCCCCCCCCCCCCCCCCCHHHHHHHHHCCEEEEEEEECCCCCCHH
KIIVKNFMEN
HHEEHHHHCC
>Mature Secondary Structure 
TNRSANMKAIKSRNTKPELMLRALLRRLGFPGYRLHRKDLPGKPDVAYIGRKKAIFVHG
CCCCCCHHHHHCCCCCHHHHHHHHHHHCCCCCCEEECCCCCCCCCEEEECCCEEEEEEE
CFWHGHPCKNGYHLPQTNPGYWGPKIERNMTRDKEHIAKLEALGWSVLIVWECELNKVDI
EEECCCCCCCCEECCCCCCCCCCCCCCCCCCCCHHHHHHHHHCCEEEEEEEECCCCCCHH
KIIVKNFMEN
HHEEHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: DNA mismatch endonuclease vsr specifically recognizes a T-G mismatch in a special context related to the dcm methylation sequence and makes an incision 5' to the mismatched T residue [C]

General reaction: Hydrolase; Acting on ester bonds [C]

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 8078464 [H]