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The map label for this gene is intS [H]

Identifier: 220903549

GI number: 220903549

Start: 312579

End: 313205

Strand: Direct

Name: intS [H]

Synonym: Ddes_0268

Alternate gene names: 220903549

Gene position: 312579-313205 (Clockwise)

Preceding gene: 220903547

Following gene: 220903550

Centisome position: 10.88

GC content: 51.99

Gene sequence:

>627_bases
ATGGCGTTGACTATCAAGGAAATCGAGGCGGCAAAACCCAAAACCAACAAGAAAACCGGCAAGCAGGCTCCGGTCAAATT
GTCCGACGAAAACGGACTGATGTTGTTCGTCACCAGCCAAAACAAAATATGGCGGGCGCGGTATTTCTACATGGGCAAGG
AGCAAAACCTGACCCTCGGCAAATATCCCCTTATGAGCCTCAAAGAAGCGCGAGAAGCGAACTTTGCATTGCGGCAACAG
TTGGACAGGGGAGAAAACCCCGCCAAGGCAAAGAAGGCCAAGCAGGAAGAACAGCTTGCCGTCTTTGCAGAAGAAAGGCG
GATCGCCAGGGGCGAGAGCCACCCGGACAGCTTTGGAGCCATAGCCGAAGACTGGCTTTCAGACGTGAAAAAAGGTTGGA
AGCGGAGAACATGCGTTGCCGAAGCAAAACGAGTCAGGAAACACCTTATTGAGCCATTGGGGCACCTTCTGATTAAGGAT
GTCAAAGCCAATGAGCATATACGCCCCTTGTTCCAGCGACTTGAGGACGCGGGCAAATATACCACCCTGAAAAAGATTGC
CGAAAAGACCGTCAGCATATTCAACTTCGCTATCGCCCTGGGCAAGACCGAGAACAACCCCGCCTAA

Upstream 100 bases:

>100_bases
CCGATTAAATCCCCCGCTTGCCTGGGTACAATCCTGGGTATACCCGGCAGATGAGCGTTCGGATTTTTAACTCTACCCAG
CTAGAGCGGAGGACACGGAA

Downstream 100 bases:

>100_bases
CCGGCAACAACGGGCCACTTTCGGCAACGAGAATTGAGGGCCGGATATGTGAAGTAGCTTACTTCGTAATCTATTCACGA
CCGGCCCTGCGGGGCTGCTC

Product: putative phage integrase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 208; Mature: 207

Protein sequence:

>208_residues
MALTIKEIEAAKPKTNKKTGKQAPVKLSDENGLMLFVTSQNKIWRARYFYMGKEQNLTLGKYPLMSLKEAREANFALRQQ
LDRGENPAKAKKAKQEEQLAVFAEERRIARGESHPDSFGAIAEDWLSDVKKGWKRRTCVAEAKRVRKHLIEPLGHLLIKD
VKANEHIRPLFQRLEDAGKYTTLKKIAEKTVSIFNFAIALGKTENNPA

Sequences:

>Translated_208_residues
MALTIKEIEAAKPKTNKKTGKQAPVKLSDENGLMLFVTSQNKIWRARYFYMGKEQNLTLGKYPLMSLKEAREANFALRQQ
LDRGENPAKAKKAKQEEQLAVFAEERRIARGESHPDSFGAIAEDWLSDVKKGWKRRTCVAEAKRVRKHLIEPLGHLLIKD
VKANEHIRPLFQRLEDAGKYTTLKKIAEKTVSIFNFAIALGKTENNPA
>Mature_207_residues
ALTIKEIEAAKPKTNKKTGKQAPVKLSDENGLMLFVTSQNKIWRARYFYMGKEQNLTLGKYPLMSLKEAREANFALRQQL
DRGENPAKAKKAKQEEQLAVFAEERRIARGESHPDSFGAIAEDWLSDVKKGWKRRTCVAEAKRVRKHLIEPLGHLLIKDV
KANEHIRPLFQRLEDAGKYTTLKKIAEKTVSIFNFAIALGKTENNPA

Specific function: Integrase is necessary for integration of the phage into the host genome by site-specific recombination. In conjunction with excisionase, integrase is also necessary for excision of the prophage from the host genome [H]

COG id: COG0582

COG function: function code L; Integrase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the 'phage' integrase family [H]

Homologues:

Organism=Escherichia coli, GI1788690, Length=207, Percent_Identity=34.7826086956522, Blast_Score=111, Evalue=5e-26,
Organism=Escherichia coli, GI145693166, Length=213, Percent_Identity=31.4553990610329, Blast_Score=65, Evalue=3e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR011010
- InterPro:   IPR013762
- InterPro:   IPR002104
- InterPro:   IPR023109 [H]

Pfam domain/function: PF00589 Phage_integrase [H]

EC number: NA

Molecular weight: Translated: 23688; Mature: 23557

Theoretical pI: Translated: 10.56; Mature: 10.56

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.5 %Cys     (Translated Protein)
1.9 %Met     (Translated Protein)
2.4 %Cys+Met (Translated Protein)
0.5 %Cys     (Mature Protein)
1.4 %Met     (Mature Protein)
1.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MALTIKEIEAAKPKTNKKTGKQAPVKLSDENGLMLFVTSQNKIWRARYFYMGKEQNLTLG
CCEEHHHHHCCCCCCCCCCCCCCCEEECCCCCEEEEEECCCCEEEHHEEEECCCCCCCCC
KYPLMSLKEAREANFALRQQLDRGENPAKAKKAKQEEQLAVFAEERRIARGESHPDSFGA
CCCHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHH
IAEDWLSDVKKGWKRRTCVAEAKRVRKHLIEPLGHLLIKDVKANEHIRPLFQRLEDAGKY
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHCCCH
TTLKKIAEKTVSIFNFAIALGKTENNPA
HHHHHHHHHHHHHHHHHHHHCCCCCCCC
>Mature Secondary Structure 
ALTIKEIEAAKPKTNKKTGKQAPVKLSDENGLMLFVTSQNKIWRARYFYMGKEQNLTLG
CEEHHHHHCCCCCCCCCCCCCCCEEECCCCCEEEEEECCCCEEEHHEEEECCCCCCCCC
KYPLMSLKEAREANFALRQQLDRGENPAKAKKAKQEEQLAVFAEERRIARGESHPDSFGA
CCCHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHH
IAEDWLSDVKKGWKRRTCVAEAKRVRKHLIEPLGHLLIKDVKANEHIRPLFQRLEDAGKY
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHCCCH
TTLKKIAEKTVSIFNFAIALGKTENNPA
HHHHHHHHHHHHHHHHHHHHCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9205837; 9278503 [H]