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The map label for this gene is glnS [H]

Identifier: 220903548

GI number: 220903548

Start: 310156

End: 311919

Strand: Reverse

Name: glnS [H]

Synonym: Ddes_0267

Alternate gene names: 220903548

Gene position: 311919-310156 (Counterclockwise)

Preceding gene: 220903555

Following gene: 220903546

Centisome position: 10.86

GC content: 58.11

Gene sequence:

>1764_bases
ATGGCCCCCGAAACAAAATCCACAGTCCCCTCCCCCGCAGCGCCCGCAGCAGGTTCCCCCAGTCCTTCTTCCAACGCCAC
TGCGGCGGAGTCTGCAAACAAGCCCGGAGTGGACTTTATCCGGGCGCGCATTATGGAAGACAACGCCACCGGCCGTTACG
GCGGCAAGGTTCATACGCGCTTTCCTCCTGAGCCCAACGGATATCTGCATCTGGGCCATGCCAAATCCATCTGCCTGAAT
TTCGGCGTTGCTAATGAATTCAATGGATTGTGCAACCTGCGCTTTGACGACACCAATCCCACCAAGGAAGAACAGGAATA
CGTAGACTCCATCCGCGAAGACGTCCACTGGCTTGGCGGCGACTGGCAGGATAGAGAGTTTTACGCTTCCAACTATTTTG
AGCAGCTGTACGAATATGCCGAACAGCTTATCAAAATGGGTAAAGCCTATGTGGACGACCTTTCGGCTGAAGAAATCCGT
GAACATCGCGGCACTCTTACCGAACCCGGCCGCGAAAGCCCCTGGCGCAACCGCAGCGTGGAAGAAAACCTCGACCTCTT
CCGCCGCATGCGTGCCGGAGAATTTGCCGATGGCGAACGGGTACTGCGCGCCAAGATCAACATGGCTTCGCCCAATCTTG
TCATGCGGGATCCGACAATCTACCGCATCCGGCATGCCGAGCACCACCGCACGGGCAACGACTGGTGCATCTACCCCATG
TATGACTTTACCCACTGCCTGTCGGACTCCATTGAAGGCATTACCCATTCACTCTGCACACTGGAGTTCGTGAACAACCG
CGAACTGTACGACTGGGTGCTCGAGACCCTCGGGGTCTACCACCCCCAGCAGATTGAATTCGCCCGCCTCAACCTCACCT
ATACCGTGCTCTCCAAGCGCAAGCTCATCCAGCTTGTCAAAGAAGGGCACGTACAGGGCTGGGACGACCCGCGCATGCCC
ACACTCAGCGGGCTGCGCCGCCGGGGTGTGCCGCCTGAAGCCCTGCGCGAATTCTGCTCCCGCATCGGCCTGGCGCGGGC
CGACTCCACCGTGGAATACTCCATGCTGGAATTCTGCGTACGTGAGCATCTGAACGCCCATGCCGCACGCGTTATGGCTG
TGCTGGATCCGATCAAGGTAGTGATAGAAAACTACCCCGAAGGGCAGGTGGAAGAATTCGACATGCCCTTCCATCCGGAG
GACGAAAGCTATGGCAGCCGCAAGGTTCCCTTCTCGCGCGAAATATATGTCGAGCGTGACGACTTCCGCCTGGACCCGCC
GAAAAAATACCACCGCCTGGCCCCTGGCGTGGAAGTACGCCTGCGCTACGCATACTTTATCACTTGCCGCGAAGCCGTGT
TGGATGAAAACGGCAATGTAACGGAGCTGCGCTGCGTGTACGACCCTGAAAGCCGTGGCGGCCAAAGTCCGGATGGCCGC
AAGGTCAAGGGAACCATCCACTGGGTGTCGGGCGCGCATGCGCTTCCGGCAGAGGTACGCCTGTACGAGCAGCTCTTCAG
CGTAGAAAACCCCAATGCGGCTCCTGAAGGTCAGTCCTTTCTGGACTATATCAATCCCCAGTCGCTTACGGTTGTTGAGG
GCCTGCTTGAGCCGGCCCTTGCAGAGCTTGCCGTGGGCCGGAAAGTGCAATTCGAGCGCCTTGGCTACTTTTGCAAGGAT
AAAGACAGCACCGCAGAGCGCCCGGTCTTTAACCGCACAGCAACCCTGCGCGACACCTGGGCCAAACTTGAGAAAAAAGG
ATAA

Upstream 100 bases:

>100_bases
ACATGGCGTATTTCTCTGCCGGGCAGCCGCACGTTGACCCCTGCCGCTGCTTGGCGTAATTTGAGAGTTTACATTACCGC
CAGGAGCAATGGAGCGCATT

Downstream 100 bases:

>100_bases
GGTCCGCAGCCCGGCTGTGGCCCCACGGCAACAACCAGCCGGGGCATACATGGCCCGGCGACATGTGGCTATTGCCGAAA
ACAGTATTGGAAAAACATAG

Product: glutaminyl-tRNA synthetase

Products: NA

Alternate protein names: Glutamine--tRNA ligase; GlnRS [H]

Number of amino acids: Translated: 587; Mature: 586

Protein sequence:

>587_residues
MAPETKSTVPSPAAPAAGSPSPSSNATAAESANKPGVDFIRARIMEDNATGRYGGKVHTRFPPEPNGYLHLGHAKSICLN
FGVANEFNGLCNLRFDDTNPTKEEQEYVDSIREDVHWLGGDWQDREFYASNYFEQLYEYAEQLIKMGKAYVDDLSAEEIR
EHRGTLTEPGRESPWRNRSVEENLDLFRRMRAGEFADGERVLRAKINMASPNLVMRDPTIYRIRHAEHHRTGNDWCIYPM
YDFTHCLSDSIEGITHSLCTLEFVNNRELYDWVLETLGVYHPQQIEFARLNLTYTVLSKRKLIQLVKEGHVQGWDDPRMP
TLSGLRRRGVPPEALREFCSRIGLARADSTVEYSMLEFCVREHLNAHAARVMAVLDPIKVVIENYPEGQVEEFDMPFHPE
DESYGSRKVPFSREIYVERDDFRLDPPKKYHRLAPGVEVRLRYAYFITCREAVLDENGNVTELRCVYDPESRGGQSPDGR
KVKGTIHWVSGAHALPAEVRLYEQLFSVENPNAAPEGQSFLDYINPQSLTVVEGLLEPALAELAVGRKVQFERLGYFCKD
KDSTAERPVFNRTATLRDTWAKLEKKG

Sequences:

>Translated_587_residues
MAPETKSTVPSPAAPAAGSPSPSSNATAAESANKPGVDFIRARIMEDNATGRYGGKVHTRFPPEPNGYLHLGHAKSICLN
FGVANEFNGLCNLRFDDTNPTKEEQEYVDSIREDVHWLGGDWQDREFYASNYFEQLYEYAEQLIKMGKAYVDDLSAEEIR
EHRGTLTEPGRESPWRNRSVEENLDLFRRMRAGEFADGERVLRAKINMASPNLVMRDPTIYRIRHAEHHRTGNDWCIYPM
YDFTHCLSDSIEGITHSLCTLEFVNNRELYDWVLETLGVYHPQQIEFARLNLTYTVLSKRKLIQLVKEGHVQGWDDPRMP
TLSGLRRRGVPPEALREFCSRIGLARADSTVEYSMLEFCVREHLNAHAARVMAVLDPIKVVIENYPEGQVEEFDMPFHPE
DESYGSRKVPFSREIYVERDDFRLDPPKKYHRLAPGVEVRLRYAYFITCREAVLDENGNVTELRCVYDPESRGGQSPDGR
KVKGTIHWVSGAHALPAEVRLYEQLFSVENPNAAPEGQSFLDYINPQSLTVVEGLLEPALAELAVGRKVQFERLGYFCKD
KDSTAERPVFNRTATLRDTWAKLEKKG
>Mature_586_residues
APETKSTVPSPAAPAAGSPSPSSNATAAESANKPGVDFIRARIMEDNATGRYGGKVHTRFPPEPNGYLHLGHAKSICLNF
GVANEFNGLCNLRFDDTNPTKEEQEYVDSIREDVHWLGGDWQDREFYASNYFEQLYEYAEQLIKMGKAYVDDLSAEEIRE
HRGTLTEPGRESPWRNRSVEENLDLFRRMRAGEFADGERVLRAKINMASPNLVMRDPTIYRIRHAEHHRTGNDWCIYPMY
DFTHCLSDSIEGITHSLCTLEFVNNRELYDWVLETLGVYHPQQIEFARLNLTYTVLSKRKLIQLVKEGHVQGWDDPRMPT
LSGLRRRGVPPEALREFCSRIGLARADSTVEYSMLEFCVREHLNAHAARVMAVLDPIKVVIENYPEGQVEEFDMPFHPED
ESYGSRKVPFSREIYVERDDFRLDPPKKYHRLAPGVEVRLRYAYFITCREAVLDENGNVTELRCVYDPESRGGQSPDGRK
VKGTIHWVSGAHALPAEVRLYEQLFSVENPNAAPEGQSFLDYINPQSLTVVEGLLEPALAELAVGRKVQFERLGYFCKDK
DSTAERPVFNRTATLRDTWAKLEKKG

Specific function: Unknown

COG id: COG0008

COG function: function code J; Glutamyl- and glutaminyl-tRNA synthetases

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the class-I aminoacyl-tRNA synthetase family [H]

Homologues:

Organism=Homo sapiens, GI4826960, Length=535, Percent_Identity=47.8504672897196, Blast_Score=463, Evalue=1e-130,
Organism=Homo sapiens, GI62241042, Length=513, Percent_Identity=36.2573099415205, Blast_Score=295, Evalue=7e-80,
Organism=Escherichia coli, GI1786895, Length=557, Percent_Identity=60.3231597845601, Blast_Score=700, Evalue=0.0,
Organism=Escherichia coli, GI1788743, Length=306, Percent_Identity=24.5098039215686, Blast_Score=67, Evalue=4e-12,
Organism=Caenorhabditis elegans, GI17543332, Length=538, Percent_Identity=45.5390334572491, Blast_Score=451, Evalue=1e-127,
Organism=Caenorhabditis elegans, GI17511007, Length=519, Percent_Identity=33.3333333333333, Blast_Score=265, Evalue=7e-71,
Organism=Saccharomyces cerevisiae, GI6324742, Length=567, Percent_Identity=42.8571428571429, Blast_Score=432, Evalue=1e-122,
Organism=Saccharomyces cerevisiae, GI6321192, Length=508, Percent_Identity=36.4173228346457, Blast_Score=313, Evalue=4e-86,
Organism=Drosophila melanogaster, GI17864488, Length=537, Percent_Identity=45.2513966480447, Blast_Score=464, Evalue=1e-130,
Organism=Drosophila melanogaster, GI24649466, Length=515, Percent_Identity=35.1456310679612, Blast_Score=273, Evalue=3e-73,

Paralogues:

None

Copy number: 520 Molecules/Cell In: Growth-Phase, Minimal-Media (Based on E. coli). 1200 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001412
- InterPro:   IPR004514
- InterPro:   IPR022861
- InterPro:   IPR000924
- InterPro:   IPR020061
- InterPro:   IPR020058
- InterPro:   IPR020059
- InterPro:   IPR020056
- InterPro:   IPR011035
- InterPro:   IPR014729 [H]

Pfam domain/function: PF00749 tRNA-synt_1c; PF03950 tRNA-synt_1c_C [H]

EC number: =6.1.1.18 [H]

Molecular weight: Translated: 67030; Mature: 66899

Theoretical pI: Translated: 5.75; Mature: 5.75

Prosite motif: PS00435 PEROXIDASE_1 ; PS00178 AA_TRNA_LIGASE_I

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.7 %Cys     (Translated Protein)
1.9 %Met     (Translated Protein)
3.6 %Cys+Met (Translated Protein)
1.7 %Cys     (Mature Protein)
1.7 %Met     (Mature Protein)
3.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAPETKSTVPSPAAPAAGSPSPSSNATAAESANKPGVDFIRARIMEDNATGRYGGKVHTR
CCCCCCCCCCCCCCCCCCCCCCCCCCCCHHCCCCCCHHHHHHHHHCCCCCCCCCCEEECC
FPPEPNGYLHLGHAKSICLNFGVANEFNGLCNLRFDDTNPTKEEQEYVDSIREDVHWLGG
CCCCCCCEEEECCHHHHHEECCCCCCCCCEEEEEECCCCCCHHHHHHHHHHHHHHHHCCC
DWQDREFYASNYFEQLYEYAEQLIKMGKAYVDDLSAEEIREHRGTLTEPGRESPWRNRSV
CCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHCCCCCCCCCCCCCCCCCH
EENLDLFRRMRAGEFADGERVLRAKINMASPNLVMRDPTIYRIRHAEHHRTGNDWCIYPM
HHHHHHHHHHHCCCCCCCHHEEHEEECCCCCCEEEECCCEEEEECHHHCCCCCCEEEEEH
YDFTHCLSDSIEGITHSLCTLEFVNNRELYDWVLETLGVYHPQQIEFARLNLTYTVLSKR
HHHHHHHHHHHHHHHHHHHHEEECCCCHHHHHHHHHHCCCCCCCEEEEEEEEEEHHHHHH
KLIQLVKEGHVQGWDDPRMPTLSGLRRRGVPPEALREFCSRIGLARADSTVEYSMLEFCV
HHHHHHHHCCCCCCCCCCCCHHHHHHHCCCCHHHHHHHHHHCCCCCCCCHHHHHHHHHHH
REHLNAHAARVMAVLDPIKVVIENYPEGQVEEFDMPFHPEDESYGSRKVPFSREIYVERD
HHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHCCCCCCCCCCCCCCCCCCCCCEEEEECC
DFRLDPPKKYHRLAPGVEVRLRYAYFITCREAVLDENGNVTELRCVYDPESRGGQSPDGR
CCCCCCCHHHHHCCCCCEEEEEEEEEEEHHHHHHCCCCCEEEEEEEECCHHCCCCCCCCC
KVKGTIHWVSGAHALPAEVRLYEQLFSVENPNAAPEGQSFLDYINPQSLTVVEGLLEPAL
EEEEEEEEECCCCCCHHHHHHHHHHHCCCCCCCCCCCHHHHHHCCCCHHHHHHHHHHHHH
AELAVGRKVQFERLGYFCKDKDSTAERPVFNRTATLRDTWAKLEKKG
HHHHCCCCEEHHHHCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHCCC
>Mature Secondary Structure 
APETKSTVPSPAAPAAGSPSPSSNATAAESANKPGVDFIRARIMEDNATGRYGGKVHTR
CCCCCCCCCCCCCCCCCCCCCCCCCCCHHCCCCCCHHHHHHHHHCCCCCCCCCCEEECC
FPPEPNGYLHLGHAKSICLNFGVANEFNGLCNLRFDDTNPTKEEQEYVDSIREDVHWLGG
CCCCCCCEEEECCHHHHHEECCCCCCCCCEEEEEECCCCCCHHHHHHHHHHHHHHHHCCC
DWQDREFYASNYFEQLYEYAEQLIKMGKAYVDDLSAEEIREHRGTLTEPGRESPWRNRSV
CCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHCCCCCCCCCCCCCCCCCH
EENLDLFRRMRAGEFADGERVLRAKINMASPNLVMRDPTIYRIRHAEHHRTGNDWCIYPM
HHHHHHHHHHHCCCCCCCHHEEHEEECCCCCCEEEECCCEEEEECHHHCCCCCCEEEEEH
YDFTHCLSDSIEGITHSLCTLEFVNNRELYDWVLETLGVYHPQQIEFARLNLTYTVLSKR
HHHHHHHHHHHHHHHHHHHHEEECCCCHHHHHHHHHHCCCCCCCEEEEEEEEEEHHHHHH
KLIQLVKEGHVQGWDDPRMPTLSGLRRRGVPPEALREFCSRIGLARADSTVEYSMLEFCV
HHHHHHHHCCCCCCCCCCCCHHHHHHHCCCCHHHHHHHHHHCCCCCCCCHHHHHHHHHHH
REHLNAHAARVMAVLDPIKVVIENYPEGQVEEFDMPFHPEDESYGSRKVPFSREIYVERD
HHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHCCCCCCCCCCCCCCCCCCCCCEEEEECC
DFRLDPPKKYHRLAPGVEVRLRYAYFITCREAVLDENGNVTELRCVYDPESRGGQSPDGR
CCCCCCCHHHHHCCCCCEEEEEEEEEEEHHHHHHCCCCCEEEEEEEECCHHCCCCCCCCC
KVKGTIHWVSGAHALPAEVRLYEQLFSVENPNAAPEGQSFLDYINPQSLTVVEGLLEPAL
EEEEEEEEECCCCCCHHHHHHHHHHHCCCCCCCCCCCHHHHHHCCCCHHHHHHHHHHHHH
AELAVGRKVQFERLGYFCKDKDSTAERPVFNRTATLRDTWAKLEKKG
HHHHCCCCEEHHHHCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 12835416 [H]