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The map label for this gene is yneJ [H]

Identifier: 220903492

GI number: 220903492

Start: 261062

End: 261979

Strand: Reverse

Name: yneJ [H]

Synonym: Ddes_0210

Alternate gene names: 220903492

Gene position: 261979-261062 (Counterclockwise)

Preceding gene: 220903500

Following gene: 220903489

Centisome position: 9.12

GC content: 60.02

Gene sequence:

>918_bases
ATGGAAATGACGGACCTGCGTACCCTTGTAAGCGTGATGGAAACAGGCAGTATCACGGCTGCGGCCAAAGCGCTGAACCG
GGTTCCTTCGGGCGTGACCATGCGCATCCTTCAACTGGAAGAAAACCTGGGCGTACAGCTTTTTTTGCGCGAAAAAAAGC
GCCTTTACCCGACTGACAAGGCGCAAAGCCTGTATCAATACGCGCTCAGGATACTGGCTCTGGCCGAAGAGGCGGAAAAC
CGCGTCCGGGGCATGGCTCCCGGCGGAAAATTCCGCATCGGCGCACTGGAAAGCGCGGCGGCGGCGCGGCTGCCCGAAGT
GCTGGCGCGACTGCACGCCGGATATCCGCAAATAGCGCTGGAACTGGTCGTGGGTACCAGCCGCTCTTTATACAAGGATG
TTCTCGAGAACAGGCTGGACGCTGTTTTTGTGGTGGACATGCCTGAAGATGACCGGCTGGAACGCATGAACGCCTTTGCG
GAGGAACTGGTGGTCATCGCCCCTGAAGGGCACAGCCCCATCCGTTACCCTGACGACATCGGGCGCAAGACCCTGCTGGC
CTTTCAGGGCGGCTGCGCCTACCAGAACCGACTGGTCAACTGGTTTCGCGCCCACGGACGGGAACCGGAGCGTATAGCCG
AGCTGGCCTCGTACCATGCCATTGTGGGGGGTGTTATAGCGGGCATGGGCGTGGGTGCCGTGCCGGATTCGGTGCTGCAC
CTGTGCCGATCTGACGGCATCCTTTCTGTACACCGTCTGGGGCATCCTCTGTGCCACGCCACCACGGAACTGATCTGGCG
CAAGGGCATGCTTTCCGCCAACATGACGGCCCTGCGCCAGTGCCTGCATAGCACGCCGGAGGTCCTGGGCAGATCCATTG
CCCCGGAAAAAAATACAGACATTCCTTTGCAAAAATGA

Upstream 100 bases:

>100_bases
AACAATTTCGGTGCTTGCCATTTGCAGGGGACTATATAATGGTAAAGATGTTCATGACAACTGAATAAAAATAACAAGGA
TATTCAATTTTGGTGAATAA

Downstream 100 bases:

>100_bases
GTCACGCCCGCTGAAAATTCCGGGCCGAGGCATGATGGCGCGTATCGGCCCGCCCAGTGAACCGGGACCGGGCGGGCAAG
CATGCTTTTATGCCGCAGCA

Product: LysR family transcriptional regulator

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 305; Mature: 305

Protein sequence:

>305_residues
MEMTDLRTLVSVMETGSITAAAKALNRVPSGVTMRILQLEENLGVQLFLREKKRLYPTDKAQSLYQYALRILALAEEAEN
RVRGMAPGGKFRIGALESAAAARLPEVLARLHAGYPQIALELVVGTSRSLYKDVLENRLDAVFVVDMPEDDRLERMNAFA
EELVVIAPEGHSPIRYPDDIGRKTLLAFQGGCAYQNRLVNWFRAHGREPERIAELASYHAIVGGVIAGMGVGAVPDSVLH
LCRSDGILSVHRLGHPLCHATTELIWRKGMLSANMTALRQCLHSTPEVLGRSIAPEKNTDIPLQK

Sequences:

>Translated_305_residues
MEMTDLRTLVSVMETGSITAAAKALNRVPSGVTMRILQLEENLGVQLFLREKKRLYPTDKAQSLYQYALRILALAEEAEN
RVRGMAPGGKFRIGALESAAAARLPEVLARLHAGYPQIALELVVGTSRSLYKDVLENRLDAVFVVDMPEDDRLERMNAFA
EELVVIAPEGHSPIRYPDDIGRKTLLAFQGGCAYQNRLVNWFRAHGREPERIAELASYHAIVGGVIAGMGVGAVPDSVLH
LCRSDGILSVHRLGHPLCHATTELIWRKGMLSANMTALRQCLHSTPEVLGRSIAPEKNTDIPLQK
>Mature_305_residues
MEMTDLRTLVSVMETGSITAAAKALNRVPSGVTMRILQLEENLGVQLFLREKKRLYPTDKAQSLYQYALRILALAEEAEN
RVRGMAPGGKFRIGALESAAAARLPEVLARLHAGYPQIALELVVGTSRSLYKDVLENRLDAVFVVDMPEDDRLERMNAFA
EELVVIAPEGHSPIRYPDDIGRKTLLAFQGGCAYQNRLVNWFRAHGREPERIAELASYHAIVGGVIAGMGVGAVPDSVLH
LCRSDGILSVHRLGHPLCHATTELIWRKGMLSANMTALRQCLHSTPEVLGRSIAPEKNTDIPLQK

Specific function: Unknown

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 HTH lysR-type DNA-binding domain [H]

Homologues:

Organism=Escherichia coli, GI1787806, Length=282, Percent_Identity=35.8156028368794, Blast_Score=189, Evalue=1e-49,
Organism=Escherichia coli, GI1788481, Length=253, Percent_Identity=28.8537549407115, Blast_Score=78, Evalue=7e-16,
Organism=Escherichia coli, GI1790208, Length=252, Percent_Identity=27.7777777777778, Blast_Score=76, Evalue=3e-15,
Organism=Escherichia coli, GI1787601, Length=180, Percent_Identity=27.7777777777778, Blast_Score=74, Evalue=1e-14,
Organism=Escherichia coli, GI87082132, Length=241, Percent_Identity=26.5560165975104, Blast_Score=72, Evalue=5e-14,
Organism=Escherichia coli, GI1788297, Length=242, Percent_Identity=24.7933884297521, Blast_Score=68, Evalue=7e-13,
Organism=Escherichia coli, GI1788887, Length=243, Percent_Identity=29.6296296296296, Blast_Score=68, Evalue=8e-13,
Organism=Escherichia coli, GI1789204, Length=234, Percent_Identity=25.6410256410256, Blast_Score=68, Evalue=9e-13,
Organism=Escherichia coli, GI1790262, Length=261, Percent_Identity=24.1379310344828, Blast_Score=66, Evalue=3e-12,
Organism=Escherichia coli, GI48994962, Length=167, Percent_Identity=28.1437125748503, Blast_Score=64, Evalue=1e-11,

Paralogues:

None

Copy number: 10-20 Molecules/Cell [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000847
- InterPro:   IPR005119
- InterPro:   IPR011991 [H]

Pfam domain/function: PF00126 HTH_1; PF03466 LysR_substrate [H]

EC number: NA

Molecular weight: Translated: 33608; Mature: 33608

Theoretical pI: Translated: 8.02; Mature: 8.02

Prosite motif: PS50931 HTH_LYSR

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.3 %Cys     (Translated Protein)
3.3 %Met     (Translated Protein)
4.6 %Cys+Met (Translated Protein)
1.3 %Cys     (Mature Protein)
3.3 %Met     (Mature Protein)
4.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MEMTDLRTLVSVMETGSITAAAKALNRVPSGVTMRILQLEENLGVQLFLREKKRLYPTDK
CCHHHHHHHHHHHHCCCHHHHHHHHHHCCCCCEEEHEEHHHCCCEEEEEEHHHHCCCCHH
AQSLYQYALRILALAEEAENRVRGMAPGGKFRIGALESAAAARLPEVLARLHAGYPQIAL
HHHHHHHHHHHHHHHHHHHHHHCCCCCCCCEEECHHHHHHHHHHHHHHHHHHCCCHHHHH
ELVVGTSRSLYKDVLENRLDAVFVVDMPEDDRLERMNAFAEELVVIAPEGHSPIRYPDDI
HHHHCCCHHHHHHHHHCCCCEEEEEECCCCHHHHHHHHHHHCEEEECCCCCCCCCCCHHH
GRKTLLAFQGGCAYQNRLVNWFRAHGREPERIAELASYHAIVGGVIAGMGVGAVPDSVLH
CCCEEEHCCCCCHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHH
LCRSDGILSVHRLGHPLCHATTELIWRKGMLSANMTALRQCLHSTPEVLGRSIAPEKNTD
HHHCCCCEEHHHCCCHHHHHHHHHHHHHCCCCCCHHHHHHHHHCCHHHHCCCCCCCCCCC
IPLQK
CCCCC
>Mature Secondary Structure
MEMTDLRTLVSVMETGSITAAAKALNRVPSGVTMRILQLEENLGVQLFLREKKRLYPTDK
CCHHHHHHHHHHHHCCCHHHHHHHHHHCCCCCEEEHEEHHHCCCEEEEEEHHHHCCCCHH
AQSLYQYALRILALAEEAENRVRGMAPGGKFRIGALESAAAARLPEVLARLHAGYPQIAL
HHHHHHHHHHHHHHHHHHHHHHCCCCCCCCEEECHHHHHHHHHHHHHHHHHHCCCHHHHH
ELVVGTSRSLYKDVLENRLDAVFVVDMPEDDRLERMNAFAEELVVIAPEGHSPIRYPDDI
HHHHCCCHHHHHHHHHCCCCEEEEEECCCCHHHHHHHHHHHCEEEECCCCCCCCCCCHHH
GRKTLLAFQGGCAYQNRLVNWFRAHGREPERIAELASYHAIVGGVIAGMGVGAVPDSVLH
CCCEEEHCCCCCHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHH
LCRSDGILSVHRLGHPLCHATTELIWRKGMLSANMTALRQCLHSTPEVLGRSIAPEKNTD
HHHCCCCEEHHHCCCHHHHHHHHHHHHHCCCCCCHHHHHHHHHCCHHHHCCCCCCCCCCC
IPLQK
CCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9097039; 9278503 [H]