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The map label for this gene is tmk

Identifier: 220903414

GI number: 220903414

Start: 164931

End: 165584

Strand: Reverse

Name: tmk

Synonym: Ddes_0128

Alternate gene names: 220903414

Gene position: 165584-164931 (Counterclockwise)

Preceding gene: 220903415

Following gene: 220903413

Centisome position: 5.76

GC content: 65.14

Gene sequence:

>654_bases
ATGTTTGTCACTTTTGAAGGTATAGAAGGCGCGGGAAAATCCACGGCCATTGACTATCTGTCAGACTACCTGCAAGCGCG
CGGCCATGACCCCGTACTGACCAGAGAGCCTGGCGGCAGTGCCCTTGGGCGTCGCCTGCGCGCCCTGCTGCTGGATGTGC
GCACCGGCGGGCTGGCCAGCCGGGCCGAACTGTTCCTGTTTCTGGCCGACCGCGCCCAGCATGTGACAGAGGTCATCCGC
CCCGCCCTTGAGGCCGGGCAGGTAGTGCTCTGCGACCGCTTCACGGACTCTACCCTGGCCTATCAGGGCTACGGGCGCGG
GCTGGACACGGAATACCTGCGCAGCCTCAATACAGCGGCCACCGGCGGGCTTGAGCCTGACCTCACCCTGTTGCTGGACC
TGCCCGTGCGCTGCGGACTGGAACGCGCCGGTGAGCGCAACCGCTCCGCCGGCATGGTCATTGCCGAGGGGCGTTTTGAC
AGTGAAAGCCTGGACTTTCATGAGCGTGTACGCCGGGGTTACCGCGCCCTGGCTGAGGAAGAACCGGAACGCTTCGCCAT
TATTGACGCCTCACAGCCTCCCGAAGACGTGGTGCTGCAATGCCGCTCGGCCATTGAGGCCTATCTGCGCCGGCGGGGAC
GGGGCCTCGACTGA

Upstream 100 bases:

>100_bases
GGACCCCCTGGCAGGCCACGCTGGGCCGCCCCGTACACCGGGCCGCGCGCACCCCGGAAAAAGCCGCCCCCGCAACCCGT
AAAAAGGCGGTCAAGGAAGA

Downstream 100 bases:

>100_bases
AAAGGACGCCTCGCAAGCGCCTTCTGGGTTCTGCCTGCGTCAGGCGCACAGTGCGCGGCTTCTGCAGGCCAGGTACGCTG
CGCGCCACCAGCATCCGCCA

Product: dTMP kinase

Products: NA

Alternate protein names: dTMP kinase

Number of amino acids: Translated: 217; Mature: 217

Protein sequence:

>217_residues
MFVTFEGIEGAGKSTAIDYLSDYLQARGHDPVLTREPGGSALGRRLRALLLDVRTGGLASRAELFLFLADRAQHVTEVIR
PALEAGQVVLCDRFTDSTLAYQGYGRGLDTEYLRSLNTAATGGLEPDLTLLLDLPVRCGLERAGERNRSAGMVIAEGRFD
SESLDFHERVRRGYRALAEEEPERFAIIDASQPPEDVVLQCRSAIEAYLRRRGRGLD

Sequences:

>Translated_217_residues
MFVTFEGIEGAGKSTAIDYLSDYLQARGHDPVLTREPGGSALGRRLRALLLDVRTGGLASRAELFLFLADRAQHVTEVIR
PALEAGQVVLCDRFTDSTLAYQGYGRGLDTEYLRSLNTAATGGLEPDLTLLLDLPVRCGLERAGERNRSAGMVIAEGRFD
SESLDFHERVRRGYRALAEEEPERFAIIDASQPPEDVVLQCRSAIEAYLRRRGRGLD
>Mature_217_residues
MFVTFEGIEGAGKSTAIDYLSDYLQARGHDPVLTREPGGSALGRRLRALLLDVRTGGLASRAELFLFLADRAQHVTEVIR
PALEAGQVVLCDRFTDSTLAYQGYGRGLDTEYLRSLNTAATGGLEPDLTLLLDLPVRCGLERAGERNRSAGMVIAEGRFD
SESLDFHERVRRGYRALAEEEPERFAIIDASQPPEDVVLQCRSAIEAYLRRRGRGLD

Specific function: Phosphorylation of dTMP to form dTDP in both de novo and salvage pathways of dTTP synthesis

COG id: COG0125

COG function: function code F; Thymidylate kinase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the thymidylate kinase family

Homologues:

Organism=Escherichia coli, GI1787340, Length=213, Percent_Identity=40.3755868544601, Blast_Score=142, Evalue=1e-35,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): KTHY_DESDA (B8J2G0)

Other databases:

- EMBL:   CP001358
- RefSeq:   YP_002478726.1
- GeneID:   7283782
- GenomeReviews:   CP001358_GR
- KEGG:   dds:Ddes_0128
- HOGENOM:   HBG626009
- ProtClustDB:   CLSK2546346
- HAMAP:   MF_00165
- InterPro:   IPR018095
- InterPro:   IPR018094
- PANTHER:   PTHR10344
- TIGRFAMs:   TIGR00041

Pfam domain/function: PF02223 Thymidylate_kin

EC number: =2.7.4.9

Molecular weight: Translated: 23956; Mature: 23956

Theoretical pI: Translated: 5.03; Mature: 5.03

Prosite motif: PS01331 THYMIDYLATE_KINASE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.4 %Cys     (Translated Protein)
0.9 %Met     (Translated Protein)
2.3 %Cys+Met (Translated Protein)
1.4 %Cys     (Mature Protein)
0.9 %Met     (Mature Protein)
2.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MFVTFEGIEGAGKSTAIDYLSDYLQARGHDPVLTREPGGSALGRRLRALLLDVRTGGLAS
CEEEECCCCCCCCHHHHHHHHHHHHHCCCCCCEEECCCCHHHHHHHHHHHHHHCCCCCHH
RAELFLFLADRAQHVTEVIRPALEAGQVVLCDRFTDSTLAYQGYGRGLDTEYLRSLNTAA
HHHHHHHHHHHHHHHHHHHHHHHHCCCEEEEECCCCCEEEECCCCCCCCHHHHHHHHHHH
TGGLEPDLTLLLDLPVRCGLERAGERNRSAGMVIAEGRFDSESLDFHERVRRGYRALAEE
CCCCCCCEEEEEECCHHHCHHHHCCCCCCCCEEEECCCCCCCCCCHHHHHHHHHHHHHHC
EPERFAIIDASQPPEDVVLQCRSAIEAYLRRRGRGLD
CCCEEEEEECCCCHHHHHHHHHHHHHHHHHHCCCCCC
>Mature Secondary Structure
MFVTFEGIEGAGKSTAIDYLSDYLQARGHDPVLTREPGGSALGRRLRALLLDVRTGGLAS
CEEEECCCCCCCCHHHHHHHHHHHHHCCCCCCEEECCCCHHHHHHHHHHHHHHCCCCCHH
RAELFLFLADRAQHVTEVIRPALEAGQVVLCDRFTDSTLAYQGYGRGLDTEYLRSLNTAA
HHHHHHHHHHHHHHHHHHHHHHHHCCCEEEEECCCCCEEEECCCCCCCCHHHHHHHHHHH
TGGLEPDLTLLLDLPVRCGLERAGERNRSAGMVIAEGRFDSESLDFHERVRRGYRALAEE
CCCCCCCEEEEEECCHHHCHHHHCCCCCCCCEEEECCCCCCCCCCHHHHHHHHHHHHHHC
EPERFAIIDASQPPEDVVLQCRSAIEAYLRRRGRGLD
CCCEEEEEECCCCHHHHHHHHHHHHHHHHHHCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA