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The map label for this gene is truB [H]

Identifier: 220903342

GI number: 220903342

Start: 73067

End: 74131

Strand: Reverse

Name: truB [H]

Synonym: Ddes_0056

Alternate gene names: 220903342

Gene position: 74131-73067 (Counterclockwise)

Preceding gene: 220903343

Following gene: 220903341

Centisome position: 2.58

GC content: 62.54

Gene sequence:

>1065_bases
ATGGTCAAAAAAGATAACGTGGCACAAGCCCGCCCTTTTCAATGCCCGGAAAGTCCGGCGCCGAACAGTCCCCTTGCGGG
AGGTTCGGGGCCGAGCGCTTACGGGCAGCATGAACAAAACGCCGACAGCGCGACGCACGGGCAAACCCGTCGTACGCCTC
CCCTGCCGCAGCAACATGGCGTACTTGTGCTCAACAAGCCTTCCGGCCCCACCTCGGCCCGTTGCCTCACCGCCATAAAG
CGGCTGGGACAAAAAAAGATCGGCCACGCCGGCACACTGGACCCTCTGGCTTCAGGTGTGCTGCTGGTGCTGCTCGGGCA
GGCCACCAAACTTTCCGGCCATCTCATGAGCGGGGGCGGCAAGGTTTATAACGGCGTGCTGCGCCTCGGGCAAACCACCG
ACACCTGGGACATCGAAGGCCAGGTTACGGCTGAAGCCCCCTGGCAGCACATCAGTGAAGACGATGTGCGGCGAGAGGTC
ACGGCATGGCTGCAACTGACAGAGCAGCCTGTTCCGTCCTATTCCGCCGCAAAACACGAAGGGCAGCCCCTGTACAAGCT
GGCCCGCAAAGGGCTTGATGCCCCGCAAAAGACAAAGTTTATGGAAATTTCACAGGCGGACGTGCTTGAAATAAGCCTGC
CGCTTGTGCGCTTTCGGGTCGCATGCAGTTCCGGCACCTATATACGCTCCCTGGCCCACAGCTTGGGGATGCGACTTGGA
TGCGGAGCCGTGCTCACGGAACTGACCCGGGAGTACAGTCACCCCTTCGGCCTTGATGTGGCCCGCGATCCCGCGGACTT
CACGGCTGACCCCACCCTGTTGCCCGGCTGCGTTATCCCCGTTGCGGATGCGCTGCCTCACTGGCCCAGGGTGGACCTCA
CGCCGGAAGAAGCCGCCCGCGTACGTAATGGCATGGCTGTGTCTTGCCGTACGCCACAGGCTGACGCCGCCCCCGGCGAC
GAACTCGCATTTCTGATGGATCAGGGGCAAGCCCTGGCCTTGGCAAAACGCGAGATCACGCCCGCCGGGCCGTGCTGGGC
CGTAATGAGGGGACTTTGGAACTAG

Upstream 100 bases:

>100_bases
CCCGGCCAGAAAATCTGCTGCACAGCCGGTCAGGCCGGGCATTATCGAGGGTATATCTTTTGACTTATACCCCGGCAAAG
GGTATGTAACGCTCTTGCGT

Downstream 100 bases:

>100_bases
GGCCGTCAAGGCCCGTAACATTGCCTTACCCAAGGAGAACTGCTGTGGTACTGGATCCTACTCAGAAAAAGTCCGTCATT
GATGCCCACGCCAAGCACGA

Product: tRNA pseudouridine synthase B

Products: pseudouridine 5'-phosphate; H2O

Alternate protein names: tRNA pseudouridine 55 synthase; Psi55 synthase; tRNA pseudouridylate synthase; tRNA-uridine isomerase [H]

Number of amino acids: Translated: 354; Mature: 354

Protein sequence:

>354_residues
MVKKDNVAQARPFQCPESPAPNSPLAGGSGPSAYGQHEQNADSATHGQTRRTPPLPQQHGVLVLNKPSGPTSARCLTAIK
RLGQKKIGHAGTLDPLASGVLLVLLGQATKLSGHLMSGGGKVYNGVLRLGQTTDTWDIEGQVTAEAPWQHISEDDVRREV
TAWLQLTEQPVPSYSAAKHEGQPLYKLARKGLDAPQKTKFMEISQADVLEISLPLVRFRVACSSGTYIRSLAHSLGMRLG
CGAVLTELTREYSHPFGLDVARDPADFTADPTLLPGCVIPVADALPHWPRVDLTPEEAARVRNGMAVSCRTPQADAAPGD
ELAFLMDQGQALALAKREITPAGPCWAVMRGLWN

Sequences:

>Translated_354_residues
MVKKDNVAQARPFQCPESPAPNSPLAGGSGPSAYGQHEQNADSATHGQTRRTPPLPQQHGVLVLNKPSGPTSARCLTAIK
RLGQKKIGHAGTLDPLASGVLLVLLGQATKLSGHLMSGGGKVYNGVLRLGQTTDTWDIEGQVTAEAPWQHISEDDVRREV
TAWLQLTEQPVPSYSAAKHEGQPLYKLARKGLDAPQKTKFMEISQADVLEISLPLVRFRVACSSGTYIRSLAHSLGMRLG
CGAVLTELTREYSHPFGLDVARDPADFTADPTLLPGCVIPVADALPHWPRVDLTPEEAARVRNGMAVSCRTPQADAAPGD
ELAFLMDQGQALALAKREITPAGPCWAVMRGLWN
>Mature_354_residues
MVKKDNVAQARPFQCPESPAPNSPLAGGSGPSAYGQHEQNADSATHGQTRRTPPLPQQHGVLVLNKPSGPTSARCLTAIK
RLGQKKIGHAGTLDPLASGVLLVLLGQATKLSGHLMSGGGKVYNGVLRLGQTTDTWDIEGQVTAEAPWQHISEDDVRREV
TAWLQLTEQPVPSYSAAKHEGQPLYKLARKGLDAPQKTKFMEISQADVLEISLPLVRFRVACSSGTYIRSLAHSLGMRLG
CGAVLTELTREYSHPFGLDVARDPADFTADPTLLPGCVIPVADALPHWPRVDLTPEEAARVRNGMAVSCRTPQADAAPGD
ELAFLMDQGQALALAKREITPAGPCWAVMRGLWN

Specific function: Responsible for synthesis of pseudouridine from uracil- 55 in the psi GC loop of transfer RNAs [H]

COG id: COG0130

COG function: function code J; Pseudouridine synthase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the pseudouridine synthase truB family. Type 1 subfamily [H]

Homologues:

Organism=Homo sapiens, GI21040257, Length=225, Percent_Identity=35.1111111111111, Blast_Score=114, Evalue=2e-25,
Organism=Escherichia coli, GI2367200, Length=193, Percent_Identity=37.3056994818653, Blast_Score=127, Evalue=8e-31,
Organism=Caenorhabditis elegans, GI17553978, Length=197, Percent_Identity=30.9644670050761, Blast_Score=77, Evalue=9e-15,
Organism=Saccharomyces cerevisiae, GI6324037, Length=168, Percent_Identity=30.952380952381, Blast_Score=86, Evalue=9e-18,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002501
- InterPro:   IPR020103
- InterPro:   IPR014780 [H]

Pfam domain/function: PF01509 TruB_N [H]

EC number: 4.2.1.70

Molecular weight: Translated: 37930; Mature: 37930

Theoretical pI: Translated: 7.84; Mature: 7.84

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.0 %Cys     (Translated Protein)
2.0 %Met     (Translated Protein)
4.0 %Cys+Met (Translated Protein)
2.0 %Cys     (Mature Protein)
2.0 %Met     (Mature Protein)
4.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MVKKDNVAQARPFQCPESPAPNSPLAGGSGPSAYGQHEQNADSATHGQTRRTPPLPQQHG
CCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCC
VLVLNKPSGPTSARCLTAIKRLGQKKIGHAGTLDPLASGVLLVLLGQATKLSGHLMSGGG
EEEEECCCCCCHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHCCCHHCCCEEECCCC
KVYNGVLRLGQTTDTWDIEGQVTAEAPWQHISEDDVRREVTAWLQLTEQPVPSYSAAKHE
HHHHHHHHCCCCCCCEECCCEEECCCCHHHCCHHHHHHHHHHHHHHHCCCCCCCCCCCCC
GQPLYKLARKGLDAPQKTKFMEISQADVLEISLPLVRFRVACSSGTYIRSLAHSLGMRLG
CCHHHHHHHHCCCCCCHHHEEECCCCCEEEHHHHHHHEEECCCCCHHHHHHHHHHCCCCC
CGAVLTELTREYSHPFGLDVARDPADFTADPTLLPGCVIPVADALPHWPRVDLTPEEAAR
HHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHCCCCCCCCCCHHHHHH
VRNGMAVSCRTPQADAAPGDELAFLMDQGQALALAKREITPAGPCWAVMRGLWN
HHCCCEEEECCCCCCCCCCCCEEEECCCCCEEEHHHHCCCCCCHHHHHHHHCCC
>Mature Secondary Structure
MVKKDNVAQARPFQCPESPAPNSPLAGGSGPSAYGQHEQNADSATHGQTRRTPPLPQQHG
CCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCC
VLVLNKPSGPTSARCLTAIKRLGQKKIGHAGTLDPLASGVLLVLLGQATKLSGHLMSGGG
EEEEECCCCCCHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHCCCHHCCCEEECCCC
KVYNGVLRLGQTTDTWDIEGQVTAEAPWQHISEDDVRREVTAWLQLTEQPVPSYSAAKHE
HHHHHHHHCCCCCCCEECCCEEECCCCHHHCCHHHHHHHHHHHHHHHCCCCCCCCCCCCC
GQPLYKLARKGLDAPQKTKFMEISQADVLEISLPLVRFRVACSSGTYIRSLAHSLGMRLG
CCHHHHHHHHCCCCCCHHHEEECCCCCEEEHHHHHHHEEECCCCCHHHHHHHHHHCCCCC
CGAVLTELTREYSHPFGLDVARDPADFTADPTLLPGCVIPVADALPHWPRVDLTPEEAAR
HHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHCCCCCCCCCCHHHHHH
VRNGMAVSCRTPQADAAPGDELAFLMDQGQALALAKREITPAGPCWAVMRGLWN
HHCCCEEEECCCCCCCCCCCCEEEECCCCCEEEHHHHCCCCCCHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: uracil; D-ribose 5-phosphate

Specific reaction: uracil + D-ribose 5-phosphate = pseudouridine 5'-phosphate + H2O

General reaction: addition of H2O; elimination of H2O; C-O bond cleavage [C]

Inhibitor: 1-(Tetrahydro-2-furanyl)-5-fluorouracil; 5-fluorouracil [C]

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA