Click here to switch to the map view.

The map label for this gene is pnp

Identifier: 220903340

GI number: 220903340

Start: 70353

End: 72584

Strand: Reverse

Name: pnp

Synonym: Ddes_0054

Alternate gene names: 220903340

Gene position: 72584-70353 (Counterclockwise)

Preceding gene: 220903341

Following gene: 220903339

Centisome position: 2.53

GC content: 62.59

Gene sequence:

>2232_bases
ATGTCACAAGATATCTTCACCCCCACCCGCGTCACCTGCCAGGTGGGCGGCAAGGAAATCATCATCGAAAGCGGCCGCAT
GGCCAATCAGGCCCACGGCGAAGTGTGGATTCAGTGCGGCGGCACCGTGGTGCTTGTCACCGTCTGCTCCCAGCCGCTTG
AATTCGACAAGGGCTTCTTTCCCCTTACCGTTGAATACTCTGAAAAAATGTACGCCGCCGGACGCATTCCCGGCAGCTTT
TTCCGCCGCGAAATCGGCCGCCCGTCCGAGCGCGAAACCCTGGTTGCGCGCCTTATCGACCGCCCCATCCGCCCGCTTTT
CCCCAAGGGGTTGAACGAGGACGTGCAGGTGCTGGCCAGCGTTATTTCTGCTGACCAGGAAAACGAGTCCGACGTGCTGG
CCCTCACCGGCGCTTCGGCGGCCCTCATGCTTTCGCCCCTGCCCTTTGAAGGCCCTGTGGCCGGTGGCCGCATTGCCCGC
GTGGGCGGTCAGTTTGTGCTCAACCCCACCTTTGAGCAGCAGGCCCAGAGCGACCTCAATATCGTCTTTGCCGCCTCCGG
CGACGCCCTCACCATGGTGGAAGGCGACGCCCGCTTTATTTCCGAAGATGTTATCATCGAGGCTCTTGAATGGGGTCGCC
TGCAGATTCTGCCCCTGGTGGAAATCCAGCACAAGCTGCGTGAACTTTGCGGCAAGCCCAAGATGAACTTCACGCCCCAC
CAGGACGATGAAGCCCTCGTGGCCTGCGTGCACGAGCTGGCCCTTGCCAACGGCATGGAAGAAGCCCTGCGCGTGCCCGA
AAAAATGGCCCGCAAAGACGCGCGCAAAGCCGTGAAAGACAAGGTCATGGAAACCCTCAAGGCCGACCCGGCCTGGGCCG
AAAACGAAGCTGCCCTCAAGGCCGTGTCCGACATCCTGGGCGGGCTTGAAAAGAAGCTCGTGCGTGCGCGCATTGTCAAC
GAAGGCACGCGCATTGACGGCCGCGACACAACCACCGTCCGGCCCATCCAGATCCAGACCGGCCTTCTGCCCCGCGCCCA
CGGCTCTGCCCTGTTCCGCCGCGGCGAAACCAAGTCTCTTGTGGTCGCCACCCTTGGCTCCTCCACAGACGAGCAGCGCA
TGGACTCCCTCACCGGCGATGTCACCAAACGGTTTATGCTGCACTACAACTTTCCGCCCTTCTCTGTGGGCGAAGTGAAG
CCCGTGCGCGTGTCCCGCCGCGAAATCGGCCACGGCGCCCTGGCGGAAAAGTCCCTGCGCCCCGTGCTGCCCGCTGACGC
CGACTTTCCCTTCACCCTGCGCGTGGTGGCCGAAACGATGGAATCCAACGGTTCTTCGTCCATGGCTGCCGTGTGCGGTG
GTTCGCTCTCCCTCATGGACGCCGGCGTGCCTGTGAGCGCCCCGGTGGCCGGTGTGGCCATGGGCCTCATCAAGGAAGGC
GAAAAGTTCATCGTTCTTACCGATATTCTGGGTGATGAAGACGCCCTGGGCGATATGGACTTCAAGATTGCCGGTACTGC
TGAAGGCGTCACCGGCGTGCAGATGGACATCAAGATCACGGGCCTTACCACCGAGATCATGCGCACCGCCATGCAGCAGG
CCCGCGAAGGCCGCCTGCATATCCTTGAAGAAATGGCCAAGGCCATCGCCTCGCCGCGCAAGGAACTTTCGCGCTACGCC
CCGCAGCATGCCGAAGTGTTCGTCAACCCTGACATCATCCGCCTGATCATTGGTCCCGGCGGCAAGAACATCAAGGCCAT
CACCGCGGCCACCGGCGCCTCTGTGGACATTGAAGACTCGGGCCGCGTGTCCATCTTCGCCCCCACGGCCGAAGCTCTGG
AAAAAGCCCGCGAAATGGTTTCCTACTACGACCAGCGCCCCGATCTTGGCAAAAACTACAATGCCAAGGTGCGCAAAATC
ATGGAAATCGGCGCCATTGTCGAAGTGCTGCCCAACGTGGAAGCCCTTGTGCACGTGTCGCAGCTTGACGTGAACCGCGT
GGAGCAACCCGGCGACGTGGCGCGCCTGGGCGAAGACATGCTGGTCAAGGTCATCGAAATCAACGGCGACCGCATCCGCG
CCAGCCGCAAGGCGGTGCTGCTGGAAGAACAGGGCCATCCCTGGAATCCTGAAGACACCGCCCGCCCGCAGCGCTCCGAC
AGGGGTGACCGTGGTGACAGGCGCGGCGACCGCGGCGGACGTGACCGCCGTGATCGTGGTGACAGGCGCTAG

Upstream 100 bases:

>100_bases
AGGGCGCAACGCCAGAAGGGGGGTCCGGGAAAATCAGGCAGGGACGTGCGGCTCTGCCCGCTTTTGCCGGAATCCCCTTC
GCCTGTGCAAAGGAAATCAC

Downstream 100 bases:

>100_bases
ACGCCGGATGGCTTTAACGCCGCTCATTGAAGCCTGAATTGAAGCGGCCCGGCCGGGCCATAGGCCCGACCGGGCGCTGA
AAGAGTGATAAAGGAAATTC

Product: polynucleotide phosphorylase/polyadenylase

Products: NA

Alternate protein names: Polynucleotide phosphorylase; PNPase

Number of amino acids: Translated: 743; Mature: 742

Protein sequence:

>743_residues
MSQDIFTPTRVTCQVGGKEIIIESGRMANQAHGEVWIQCGGTVVLVTVCSQPLEFDKGFFPLTVEYSEKMYAAGRIPGSF
FRREIGRPSERETLVARLIDRPIRPLFPKGLNEDVQVLASVISADQENESDVLALTGASAALMLSPLPFEGPVAGGRIAR
VGGQFVLNPTFEQQAQSDLNIVFAASGDALTMVEGDARFISEDVIIEALEWGRLQILPLVEIQHKLRELCGKPKMNFTPH
QDDEALVACVHELALANGMEEALRVPEKMARKDARKAVKDKVMETLKADPAWAENEAALKAVSDILGGLEKKLVRARIVN
EGTRIDGRDTTTVRPIQIQTGLLPRAHGSALFRRGETKSLVVATLGSSTDEQRMDSLTGDVTKRFMLHYNFPPFSVGEVK
PVRVSRREIGHGALAEKSLRPVLPADADFPFTLRVVAETMESNGSSSMAAVCGGSLSLMDAGVPVSAPVAGVAMGLIKEG
EKFIVLTDILGDEDALGDMDFKIAGTAEGVTGVQMDIKITGLTTEIMRTAMQQAREGRLHILEEMAKAIASPRKELSRYA
PQHAEVFVNPDIIRLIIGPGGKNIKAITAATGASVDIEDSGRVSIFAPTAEALEKAREMVSYYDQRPDLGKNYNAKVRKI
MEIGAIVEVLPNVEALVHVSQLDVNRVEQPGDVARLGEDMLVKVIEINGDRIRASRKAVLLEEQGHPWNPEDTARPQRSD
RGDRGDRRGDRGGRDRRDRGDRR

Sequences:

>Translated_743_residues
MSQDIFTPTRVTCQVGGKEIIIESGRMANQAHGEVWIQCGGTVVLVTVCSQPLEFDKGFFPLTVEYSEKMYAAGRIPGSF
FRREIGRPSERETLVARLIDRPIRPLFPKGLNEDVQVLASVISADQENESDVLALTGASAALMLSPLPFEGPVAGGRIAR
VGGQFVLNPTFEQQAQSDLNIVFAASGDALTMVEGDARFISEDVIIEALEWGRLQILPLVEIQHKLRELCGKPKMNFTPH
QDDEALVACVHELALANGMEEALRVPEKMARKDARKAVKDKVMETLKADPAWAENEAALKAVSDILGGLEKKLVRARIVN
EGTRIDGRDTTTVRPIQIQTGLLPRAHGSALFRRGETKSLVVATLGSSTDEQRMDSLTGDVTKRFMLHYNFPPFSVGEVK
PVRVSRREIGHGALAEKSLRPVLPADADFPFTLRVVAETMESNGSSSMAAVCGGSLSLMDAGVPVSAPVAGVAMGLIKEG
EKFIVLTDILGDEDALGDMDFKIAGTAEGVTGVQMDIKITGLTTEIMRTAMQQAREGRLHILEEMAKAIASPRKELSRYA
PQHAEVFVNPDIIRLIIGPGGKNIKAITAATGASVDIEDSGRVSIFAPTAEALEKAREMVSYYDQRPDLGKNYNAKVRKI
MEIGAIVEVLPNVEALVHVSQLDVNRVEQPGDVARLGEDMLVKVIEINGDRIRASRKAVLLEEQGHPWNPEDTARPQRSD
RGDRGDRRGDRGGRDRRDRGDRR
>Mature_742_residues
SQDIFTPTRVTCQVGGKEIIIESGRMANQAHGEVWIQCGGTVVLVTVCSQPLEFDKGFFPLTVEYSEKMYAAGRIPGSFF
RREIGRPSERETLVARLIDRPIRPLFPKGLNEDVQVLASVISADQENESDVLALTGASAALMLSPLPFEGPVAGGRIARV
GGQFVLNPTFEQQAQSDLNIVFAASGDALTMVEGDARFISEDVIIEALEWGRLQILPLVEIQHKLRELCGKPKMNFTPHQ
DDEALVACVHELALANGMEEALRVPEKMARKDARKAVKDKVMETLKADPAWAENEAALKAVSDILGGLEKKLVRARIVNE
GTRIDGRDTTTVRPIQIQTGLLPRAHGSALFRRGETKSLVVATLGSSTDEQRMDSLTGDVTKRFMLHYNFPPFSVGEVKP
VRVSRREIGHGALAEKSLRPVLPADADFPFTLRVVAETMESNGSSSMAAVCGGSLSLMDAGVPVSAPVAGVAMGLIKEGE
KFIVLTDILGDEDALGDMDFKIAGTAEGVTGVQMDIKITGLTTEIMRTAMQQAREGRLHILEEMAKAIASPRKELSRYAP
QHAEVFVNPDIIRLIIGPGGKNIKAITAATGASVDIEDSGRVSIFAPTAEALEKAREMVSYYDQRPDLGKNYNAKVRKIM
EIGAIVEVLPNVEALVHVSQLDVNRVEQPGDVARLGEDMLVKVIEINGDRIRASRKAVLLEEQGHPWNPEDTARPQRSDR
GDRGDRRGDRGGRDRRDRGDRR

Specific function: Involved in mRNA degradation. Hydrolyzes single-stranded polyribonucleotides processively in the 3'- to 5'-direction

COG id: COG1185

COG function: function code J; Polyribonucleotide nucleotidyltransferase (polynucleotide phosphorylase)

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 S1 motif domain

Homologues:

Organism=Homo sapiens, GI188528628, Length=720, Percent_Identity=38.6111111111111, Blast_Score=452, Evalue=1e-127,
Organism=Escherichia coli, GI145693187, Length=704, Percent_Identity=46.3068181818182, Blast_Score=604, Evalue=1e-174,
Organism=Caenorhabditis elegans, GI115534063, Length=712, Percent_Identity=35.1123595505618, Blast_Score=338, Evalue=6e-93,
Organism=Drosophila melanogaster, GI281362905, Length=696, Percent_Identity=37.5, Blast_Score=428, Evalue=1e-120,
Organism=Drosophila melanogaster, GI24651641, Length=696, Percent_Identity=37.5, Blast_Score=428, Evalue=1e-120,
Organism=Drosophila melanogaster, GI24651643, Length=696, Percent_Identity=37.5, Blast_Score=428, Evalue=1e-120,
Organism=Drosophila melanogaster, GI161079377, Length=641, Percent_Identity=37.2854914196568, Blast_Score=398, Evalue=1e-111,

Paralogues:

None

Copy number: 200 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 1000 Molecules/Cell In: Growth-Phase, Minimal-Media (Based on E. coli). 3328 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. 3,000 Molecules/Cell In: Glucose minimal media

Swissprot (AC and ID): PNP_DESDA (B8J1X9)

Other databases:

- EMBL:   CP001358
- RefSeq:   YP_002478652.1
- GeneID:   7283705
- GenomeReviews:   CP001358_GR
- KEGG:   dds:Ddes_0054
- HOGENOM:   HBG382411
- ProtClustDB:   PRK11824
- GO:   GO:0005739
- HAMAP:   MF_01595
- InterPro:   IPR001247
- InterPro:   IPR015847
- InterPro:   IPR004087
- InterPro:   IPR004088
- InterPro:   IPR018111
- InterPro:   IPR012340
- InterPro:   IPR016027
- InterPro:   IPR012162
- InterPro:   IPR015848
- InterPro:   IPR003029
- InterPro:   IPR020568
- InterPro:   IPR022967
- Gene3D:   G3DSA:2.40.50.140
- Gene3D:   G3DSA:1.10.10.400
- PANTHER:   PTHR11252
- PIRSF:   PIRSF005499
- SMART:   SM00322
- SMART:   SM00316
- TIGRFAMs:   TIGR03591

Pfam domain/function: PF00013 KH_1; PF03726 PNPase; PF01138 RNase_PH; PF03725 RNase_PH_C; PF00575 S1; SSF46915 3_ExoRNase; SSF55666 3_ExoRNase; SSF50249 Nucleic_acid_OB; SSF54211 Ribosomal_S5_D2-typ_fold

EC number: =2.7.7.8

Molecular weight: Translated: 81065; Mature: 80934

Theoretical pI: Translated: 5.66; Mature: 5.66

Prosite motif: PS50084 KH_TYPE_1; PS50126 S1

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
3.1 %Met     (Translated Protein)
3.9 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
3.0 %Met     (Mature Protein)
3.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSQDIFTPTRVTCQVGGKEIIIESGRMANQAHGEVWIQCGGTVVLVTVCSQPLEFDKGFF
CCCCCCCCCEEEEEECCEEEEEECCCCCCCCCCEEEEEECCEEEEEEECCCCCCCCCCCE
PLTVEYSEKMYAAGRIPGSFFRREIGRPSERETLVARLIDRPIRPLFPKGLNEDVQVLAS
EEEEECCCCCCCCCCCCHHHHHHHCCCCCHHHHHHHHHHHCCCCCCCCCCCCHHHHHHHH
VISADQENESDVLALTGASAALMLSPLPFEGPVAGGRIARVGGQFVLNPTFEQQAQSDLN
HHHCCCCCCCCEEEEECCCEEEEECCCCCCCCCCCCEEEEECCEEEECCCHHHHCCCCCE
IVFAASGDALTMVEGDARFISEDVIIEALEWGRLQILPLVEIQHKLRELCGKPKMNFTPH
EEEEECCCEEEEEECCHHHHHHHHHHHHHCCCCEEEEEHHHHHHHHHHHHCCCCCCCCCC
QDDEALVACVHELALANGMEEALRVPEKMARKDARKAVKDKVMETLKADPAWAENEAALK
CCCHHHHHHHHHHHHHCCHHHHHHCHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCHHHHH
AVSDILGGLEKKLVRARIVNEGTRIDGRDTTTVRPIQIQTGLLPRAHGSALFRRGETKSL
HHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCEEEEEEEECCCCCCCCCHHHHHCCCCCEE
VVATLGSSTDEQRMDSLTGDVTKRFMLHYNFPPFSVGEVKPVRVSRREIGHGALAEKSLR
EEEECCCCCHHHHHHHHHHHHHHHEEEEECCCCCCCCCCCCEEHHHHHCCCCHHHHHCCC
PVLPADADFPFTLRVVAETMESNGSSSMAAVCGGSLSLMDAGVPVSAPVAGVAMGLIKEG
CCCCCCCCCCCHHHHHHHHHHCCCCCCCHHHHCCCEEEEECCCCCCCCHHHHHHHHHHCC
EKFIVLTDILGDEDALGDMDFKIAGTAEGVTGVQMDIKITGLTTEIMRTAMQQAREGRLH
CEEEEEEEECCCCCCCCCCCEEEECCCCCCCEEEEEEEEECCHHHHHHHHHHHHHCCHHH
ILEEMAKAIASPRKELSRYAPQHAEVFVNPDIIRLIIGPGGKNIKAITAATGASVDIEDS
HHHHHHHHHHCHHHHHHHHCCCCCEEEECCCEEEEEECCCCCCEEEEEEECCCEEEECCC
GRVSIFAPTAEALEKAREMVSYYDQRPDLGKNYNAKVRKIMEIGAIVEVLPNVEALVHVS
CCEEEEECCHHHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHHHHCCHHHHEEHH
QLDVNRVEQPGDVARLGEDMLVKVIEINGDRIRASRKAVLLEEQGHPWNPEDTARPQRSD
HHCHHHCCCCCHHHHCCHHHEEEEEEECCCEEECCCCEEEEECCCCCCCCCCCCCCCCCC
RGDRGDRRGDRGGRDRRDRGDRR
CCCCCCCCCCCCCCCCCCCCCCC
>Mature Secondary Structure 
SQDIFTPTRVTCQVGGKEIIIESGRMANQAHGEVWIQCGGTVVLVTVCSQPLEFDKGFF
CCCCCCCCEEEEEECCEEEEEECCCCCCCCCCEEEEEECCEEEEEEECCCCCCCCCCCE
PLTVEYSEKMYAAGRIPGSFFRREIGRPSERETLVARLIDRPIRPLFPKGLNEDVQVLAS
EEEEECCCCCCCCCCCCHHHHHHHCCCCCHHHHHHHHHHHCCCCCCCCCCCCHHHHHHHH
VISADQENESDVLALTGASAALMLSPLPFEGPVAGGRIARVGGQFVLNPTFEQQAQSDLN
HHHCCCCCCCCEEEEECCCEEEEECCCCCCCCCCCCEEEEECCEEEECCCHHHHCCCCCE
IVFAASGDALTMVEGDARFISEDVIIEALEWGRLQILPLVEIQHKLRELCGKPKMNFTPH
EEEEECCCEEEEEECCHHHHHHHHHHHHHCCCCEEEEEHHHHHHHHHHHHCCCCCCCCCC
QDDEALVACVHELALANGMEEALRVPEKMARKDARKAVKDKVMETLKADPAWAENEAALK
CCCHHHHHHHHHHHHHCCHHHHHHCHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCHHHHH
AVSDILGGLEKKLVRARIVNEGTRIDGRDTTTVRPIQIQTGLLPRAHGSALFRRGETKSL
HHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCEEEEEEEECCCCCCCCCHHHHHCCCCCEE
VVATLGSSTDEQRMDSLTGDVTKRFMLHYNFPPFSVGEVKPVRVSRREIGHGALAEKSLR
EEEECCCCCHHHHHHHHHHHHHHHEEEEECCCCCCCCCCCCEEHHHHHCCCCHHHHHCCC
PVLPADADFPFTLRVVAETMESNGSSSMAAVCGGSLSLMDAGVPVSAPVAGVAMGLIKEG
CCCCCCCCCCCHHHHHHHHHHCCCCCCCHHHHCCCEEEEECCCCCCCCHHHHHHHHHHCC
EKFIVLTDILGDEDALGDMDFKIAGTAEGVTGVQMDIKITGLTTEIMRTAMQQAREGRLH
CEEEEEEEECCCCCCCCCCCEEEECCCCCCCEEEEEEEEECCHHHHHHHHHHHHHCCHHH
ILEEMAKAIASPRKELSRYAPQHAEVFVNPDIIRLIIGPGGKNIKAITAATGASVDIEDS
HHHHHHHHHHCHHHHHHHHCCCCCEEEECCCEEEEEECCCCCCEEEEEEECCCEEEECCC
GRVSIFAPTAEALEKAREMVSYYDQRPDLGKNYNAKVRKIMEIGAIVEVLPNVEALVHVS
CCEEEEECCHHHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHHHHCCHHHHEEHH
QLDVNRVEQPGDVARLGEDMLVKVIEINGDRIRASRKAVLLEEQGHPWNPEDTARPQRSD
HHCHHHCCCCCHHHHCCHHHEEEEEEECCCEEECCCCEEEEECCCCCCCCCCCCCCCCCC
RGDRGDRRGDRGGRDRRDRGDRR
CCCCCCCCCCCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA