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The map label for this gene is celB3 [H]

Identifier: 218906390

GI number: 218906390

Start: 4997251

End: 4998552

Strand: Reverse

Name: celB3 [H]

Synonym: BCAH820_5304

Alternate gene names: 218906390

Gene position: 4998552-4997251 (Counterclockwise)

Preceding gene: 218906391

Following gene: 218906389

Centisome position: 94.26

GC content: 37.86

Gene sequence:

>1302_bases
ATGATACAGTTTTTAGAGAAATATGTAATGCCAGTAGCAGGGAAGGTTGCAGAGCAGAGGCATTTGCAAGCAATTCGAGA
TGGAATTATTTTAACGATGCCTTTTTTAATCATTGGATCATTTTTCCTTATTATTAGTGCATTACCAATACCAGGTTATA
ATGATTTTATGGCAGGTTTGTTTGGGGAGAATTGGCAGAGGGCTTTAGGGTATCCAGTTAGTGCGACTTTTAATATAATG
GCTTTAATAGCTGTTTTTGGAATCGCTTACAGGCTTGGGGAATATTATAAAGTGGATGCTTTAGCATCCGGAGCATTGTC
CCTTGTGACGTTTTTACTTGCGACGCCATTTCAAGTTGCATATATTATACCAAGTACAAAAGAGAGTGTACTTGTAGAAG
GTGCTATCCCAGCTGCATTAATGGGAAGCCAAGGGTTGTTTGTAGCAATGATTATTGCACTTATATCTACTGAACTTTAT
CGGTTTATTGTACAAAAAAAGATAATTATAAAGATGCCAGAAACAGTTCCACCAGCCGTGACACGTTCATTTGCAGCACT
TGTTCCAGGATTTATTGTTGTAACGGTTATTTGGATTGTACGTTTAATTATAGAAAATACTTCTTTTGGCAGTATCCATA
ATATTGTAGGGCAAATTTTACAGGAACCACTTAGTATACTTGGTGCTAGTCTTTGGGGCGCAATAATAGCAGTTATTCTC
GTTCATGTCCTTTGGTCTTGTGGAATTCATGGTGCTACTATTGTTGGTGGTGTAATGAGTCCTGTTTGGTTATCGTTAAT
GGATCAAAACCGAGTTGCTTTCCAAGCGGGGCAAGATGTACCAAATACGATTACCGCACAGTTTTTTGATTTATGGATTT
ATATGGGCGGTTCCGGCGCAACACTAGCTTTAGTTGTCGGAATGTTATTGTTTGCACGAAGTCAGCAATTAAAAAGTTTA
GGGAGATTGTCAATTGCACCTGGTATATTTAATATTAATGAGATGGTAACTTTTGGTATGCCGATTGTAATGAACCCAAT
TTTATTAATTCCATTTATATTAGTTCCGGTTGTGTTAACGATTGTTTCTTACTTTGCAATGGAATGGGGATTAGTTGCTC
GCCCGAGTGGAGCTGCTGTACCTTGGACGACACCTATTCTTTTTAGTGGATATTTAGGATCGGGCGGGAAAATTTCAGGC
GTTGTTTTACAACTTGTTAACTTTGCGCTTGCATTCTTCATTTATTTACCGTTCTTAAAAATATGGGATAAACAAAAAGT
AGCGGAAGAAAAGGGGGAGTAA

Upstream 100 bases:

>100_bases
TACCTGTTGATGTCATTCAATCCGTCCATTACGGACTTTGTAATGGGGAAGCTATCTTGCAAGCAGCGTTGTCAATGAAA
CCATGAGGAGAGTGGGATAG

Downstream 100 bases:

>100_bases
AGAAGGAAAATGGATACGATAGAGACGAAAGCTTTTCATTTGATTTTGCATGGGGGAAATGCGAGAAGTTGTTCAATGGA
AGCAATTGATTGCGCGAAGC

Product: PTS system, cellobiose-specific IIC component

Products: protein histidine; sugar phosphate

Alternate protein names: EIIC-Cel; PTS system cellobiose-specific EIIC component [H]

Number of amino acids: Translated: 433; Mature: 433

Protein sequence:

>433_residues
MIQFLEKYVMPVAGKVAEQRHLQAIRDGIILTMPFLIIGSFFLIISALPIPGYNDFMAGLFGENWQRALGYPVSATFNIM
ALIAVFGIAYRLGEYYKVDALASGALSLVTFLLATPFQVAYIIPSTKESVLVEGAIPAALMGSQGLFVAMIIALISTELY
RFIVQKKIIIKMPETVPPAVTRSFAALVPGFIVVTVIWIVRLIIENTSFGSIHNIVGQILQEPLSILGASLWGAIIAVIL
VHVLWSCGIHGATIVGGVMSPVWLSLMDQNRVAFQAGQDVPNTITAQFFDLWIYMGGSGATLALVVGMLLFARSQQLKSL
GRLSIAPGIFNINEMVTFGMPIVMNPILLIPFILVPVVLTIVSYFAMEWGLVARPSGAAVPWTTPILFSGYLGSGGKISG
VVLQLVNFALAFFIYLPFLKIWDKQKVAEEKGE

Sequences:

>Translated_433_residues
MIQFLEKYVMPVAGKVAEQRHLQAIRDGIILTMPFLIIGSFFLIISALPIPGYNDFMAGLFGENWQRALGYPVSATFNIM
ALIAVFGIAYRLGEYYKVDALASGALSLVTFLLATPFQVAYIIPSTKESVLVEGAIPAALMGSQGLFVAMIIALISTELY
RFIVQKKIIIKMPETVPPAVTRSFAALVPGFIVVTVIWIVRLIIENTSFGSIHNIVGQILQEPLSILGASLWGAIIAVIL
VHVLWSCGIHGATIVGGVMSPVWLSLMDQNRVAFQAGQDVPNTITAQFFDLWIYMGGSGATLALVVGMLLFARSQQLKSL
GRLSIAPGIFNINEMVTFGMPIVMNPILLIPFILVPVVLTIVSYFAMEWGLVARPSGAAVPWTTPILFSGYLGSGGKISG
VVLQLVNFALAFFIYLPFLKIWDKQKVAEEKGE
>Mature_433_residues
MIQFLEKYVMPVAGKVAEQRHLQAIRDGIILTMPFLIIGSFFLIISALPIPGYNDFMAGLFGENWQRALGYPVSATFNIM
ALIAVFGIAYRLGEYYKVDALASGALSLVTFLLATPFQVAYIIPSTKESVLVEGAIPAALMGSQGLFVAMIIALISTELY
RFIVQKKIIIKMPETVPPAVTRSFAALVPGFIVVTVIWIVRLIIENTSFGSIHNIVGQILQEPLSILGASLWGAIIAVIL
VHVLWSCGIHGATIVGGVMSPVWLSLMDQNRVAFQAGQDVPNTITAQFFDLWIYMGGSGATLALVVGMLLFARSQQLKSL
GRLSIAPGIFNINEMVTFGMPIVMNPILLIPFILVPVVLTIVSYFAMEWGLVARPSGAAVPWTTPILFSGYLGSGGKISG
VVLQLVNFALAFFIYLPFLKIWDKQKVAEEKGE

Specific function: The phosphoenolpyruvate-dependent sugar phosphotransferase system (PTS), a major carbohydrate active -transport system, catalyzes the phosphorylation of incoming sugar substrates concomitant with their translocation across the cell membrane. This system i

COG id: COG1455

COG function: function code G; Phosphotransferase system cellobiose-specific component IIC

Gene ontology:

Cell location: Cell membrane; Multi-pass membrane protein (Probable) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 PTS EIIC type-3 domain [H]

Homologues:

Organism=Escherichia coli, GI1788032, Length=468, Percent_Identity=33.3333333333333, Blast_Score=224, Evalue=6e-60,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003352
- InterPro:   IPR004796
- InterPro:   IPR004501 [H]

Pfam domain/function: PF02378 PTS_EIIC [H]

EC number: 2.7.1.69

Molecular weight: Translated: 47061; Mature: 47061

Theoretical pI: Translated: 8.81; Mature: 8.81

Prosite motif: PS51105 PTS_EIIC_TYPE_3

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.2 %Cys     (Translated Protein)
3.7 %Met     (Translated Protein)
3.9 %Cys+Met (Translated Protein)
0.2 %Cys     (Mature Protein)
3.7 %Met     (Mature Protein)
3.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MIQFLEKYVMPVAGKVAEQRHLQAIRDGIILTMPFLIIGSFFLIISALPIPGYNDFMAGL
CHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHH
FGENWQRALGYPVSATFNIMALIAVFGIAYRLGEYYKVDALASGALSLVTFLLATPFQVA
HCCCHHHHHCCCCHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHCCCCEE
YIIPSTKESVLVEGAIPAALMGSQGLFVAMIIALISTELYRFIVQKKIIIKMPETVPPAV
EEECCCCCCEEEECCCCHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHEECCCCCCHHH
TRSFAALVPGFIVVTVIWIVRLIIENTSFGSIHNIVGQILQEPLSILGASLWGAIIAVIL
HHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
VHVLWSCGIHGATIVGGVMSPVWLSLMDQNRVAFQAGQDVPNTITAQFFDLWIYMGGSGA
HHHHHHCCCCHHHHHHHHHHHHHHHHHHCCCHHHHCCCCCCHHHHHHHHHHHHEECCCCH
TLALVVGMLLFARSQQLKSLGRLSIAPGIFNINEMVTFGMPIVMNPILLIPFILVPVVLT
HHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCHHHHHHHCCHHHHHHHHHHHHHHHHHHHH
IVSYFAMEWGLVARPSGAAVPWTTPILFSGYLGSGGKISGVVLQLVNFALAFFIYLPFLK
HHHHHHHHCCCEECCCCCCCCCCCHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHH
IWDKQKVAEEKGE
HHHHHHHHHCCCC
>Mature Secondary Structure
MIQFLEKYVMPVAGKVAEQRHLQAIRDGIILTMPFLIIGSFFLIISALPIPGYNDFMAGL
CHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHH
FGENWQRALGYPVSATFNIMALIAVFGIAYRLGEYYKVDALASGALSLVTFLLATPFQVA
HCCCHHHHHCCCCHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHCCCCEE
YIIPSTKESVLVEGAIPAALMGSQGLFVAMIIALISTELYRFIVQKKIIIKMPETVPPAV
EEECCCCCCEEEECCCCHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHEECCCCCCHHH
TRSFAALVPGFIVVTVIWIVRLIIENTSFGSIHNIVGQILQEPLSILGASLWGAIIAVIL
HHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
VHVLWSCGIHGATIVGGVMSPVWLSLMDQNRVAFQAGQDVPNTITAQFFDLWIYMGGSGA
HHHHHHCCCCHHHHHHHHHHHHHHHHHHCCCHHHHCCCCCCHHHHHHHHHHHHEECCCCH
TLALVVGMLLFARSQQLKSLGRLSIAPGIFNINEMVTFGMPIVMNPILLIPFILVPVVLT
HHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCHHHHHHHCCHHHHHHHHHHHHHHHHHHHH
IVSYFAMEWGLVARPSGAAVPWTTPILFSGYLGSGGKISGVVLQLVNFALAFFIYLPFLK
HHHHHHHHCCCEECCCCCCCCCCCHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHH
IWDKQKVAEEKGE
HHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: protein N(pi)-phosphohistidine; sugar

Specific reaction: protein N(pi)-phosphohistidine + sugar = protein histidine + sugar phosphate

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 8407820 [H]