| Definition | Bacillus cereus AH820, complete genome. |
|---|---|
| Accession | NC_011773 |
| Length | 5,302,683 |
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The map label for this gene is polA [H]
Identifier: 218905814
GI number: 218905814
Start: 4454028
End: 4456661
Strand: Reverse
Name: polA [H]
Synonym: BCAH820_4702
Alternate gene names: 218905814
Gene position: 4456661-4454028 (Counterclockwise)
Preceding gene: 218905815
Following gene: 218905813
Centisome position: 84.05
GC content: 36.67
Gene sequence:
>2634_bases TTGGAAAAAAAGGTCGTATTAGTAGATGGTAATAATATCGCGTATCGTGCTTTCTTTGCACTACCGCTTTTAAATAACGA CAAAGGTATACATACGAACGCAATTTACGGTTTTACAATGATGTTAATGAGAATATTAGAGGAAGAAAAACCGACGCATA TGTTAGTAGCATTTGATGCGGGGAAAACGACATTCCGTCATAAAACATATAGTGAGTATAAAGGGGGACGTCAAAAGACT CCGCCTGAGTTATCAGAGCAATTCCCGTTTATTCGTGAGATGCTTGATGCATTCAATGTACCACGTTATGAATTAGAAAA TTATGAAGCGGATGACATTATGGGAACGCTAGCGAAAGAAGCGAGTGAACAAGGCGCAAGTGTAAAAGTTATTTCAGGAG ATAAAGACTTACTTCAACTTGTTTCTGATAACACGCTTGTATGTATTCCTCGAAAAGGGATTACGGAAGTAGACGAATAT ACGAAAGAAGCTTTATTTGAAAAATACAGCTTATCACCAAAGCAAATTATCGATATGAAAGGTTTAATGGGAGACCAATC AGATAATATTCCAGGTGTACCAGGAGTTGGTGAAAAAACTGCGATTAAATTGTTAACACAGTTTGGAACGGTTGAAGAAG TGTATGAAAATATAGATCAAGTAAGCGGGAAGAAATTAAAAGAAAAGCTGGAAGCAAATAAAGACCAAGCTCTTATGAGT AAAGATCTTGCAACCATTATTACAGACGCACCGATTACTGTGAATGTGGATGATATGGAGTATAAAGGATATGAAGCAAG TGATGTCATTCCAATGTTCGAGAATTTAGGATTTACATCTCTTTTAAACAAATTAGGTGTTACGCCAGAAGAAACAGCTC CGGCTGAATTAGATGATATTACATTTGATATTGTAGAAGAAGTTACAGAAGAAATGCTTCAGCAAGATAGTGCGCTTATC GTTGAAGTACAAGAAGATAACTATCATAAAGCAGACATTCAAGGTTTCGGTATTCAAAATGAAAATGGATGTTACTTTAT TCAGACAGACATTGCACTTAAATCAGATGCTTTTAAAGAGTGGCTTGCAGATGGAGAAATGAGAAAGTATACATTTGATG CGAAGCGTGCGATCGTTGCGCTGAAATGGAACGGTATAGATATGCAAGGGATTGACTTTGATCTATTAATCGCTGCTTAC TTACTTGATCCGGCTGATACAGATAAAGATTTCCGTACTGTAGCGAAAATGAAAGAAACGCATGCTGTGAAATCTGATGA AGAAGTTTACGGAAAAGGTGCGAAGCGTGCTGTTCCAGAGTTAGAGATAGTAGCGGAGCATGTAGCTCGTAAAGTGCATG TATTATATGATGTAAAGCAAACATTTGTTGAAGAGTTAGAAAAGAATGAGCAATATGAACTGTTTACAGAGTTGGAATTA CCACTTGCACGTGTATTAGCTGATATGGAAGTAAAAGGTGTAAAAGTTGATACAGAACGTCTTCGTAATATGGGAGAAGA ACTTGCAGGTAGATTAAAGGAAATGGAACAGGAAATTTATAAGCTGGCGGGAACAGAATTTAATATTAATTCACCGAAGC AGCTTGGTGTGATTCTGTTTGAGAATTTAAACTTACCGGTTATTAAGAAGACGAAAACAGGTTATTCTACATCAGCAGAT GTATTAGACAAGCTGATGGATCACCATGAAATTATTCCAAACATTTTACATTACCGTCAATTAGGGAAACTCAATTCAAC TTATATTGAAGGTTTATTAAAAGTTGTACATGAAGATTCATCTAAAATTCATACTCGCTTCAATCAAGTATTAACGCAAA CAGGTCGATTAAGTTCAACGGATCCAAACTTGCAAAATATCCCGATTCGATTAGAAGAAGGAAGAAAGATTCGTCAGGCA TTCGTTCCATCAGAAGAAGGATGGATTATGTACGCGGCCGATTATTCACAAATTGAACTTCGTGTATTAGCTCATATTGC CAATGATAAAGGGCTAGTTGAAGCGTTCCAACATGACATGGATATTCATACAAAAACAGCGATGGATGTATTTGGCGTTG AAAAAGATGAAGTAACTTCAAATATGAGACGACAAGCGAAAGCTGTTAACTTCGGAATTGTGTATGGTATTAGTGATTAT GGTCTTTCACAAAACTTAGGAATTACAAGAAAAGCAGCAGCGGAATTTATTGAAAAGTATTTAGAAAGTTTCCCTGGTGT ACAAGAATATATGGATGACATCGTAAAAGATGCGAAGCAAAAAGGATATGTGGCTACATTATTAAATCGTCGCCGTTACA TTCCGGAAATTACGAGTCGTAATTTCAACTTGCGTAGCTTTGCTGAGCGTACAGCTATGAATACACCAATTCAAGGTACT GCAGCAGATATTATTAAAAAAGCGATGATTATTATGGCAGATCGTTTAGAAGAAGAAGGATTACAAGCGCGTCTTCTTCT GCAAGTACACGATGAATTAATATTTGAGGCACCAAAAGAAGAAGTTGAAAAATTAGAGAAGCTTGTACCAGAAGTAATGG AGCATGCAATTGAACTGGCAGTTCCACTGAAAGTTGATTATTCTTACGGTCCAACTTGGTACGACGCAAAATAA
Upstream 100 bases:
>100_bases TTTTCTTCTCTCTATTAGTCATGTTAATATAGAGAGAGGAAAGAAACGGTTTTTTTCTGTGAAAAAAATCATTTCAAATT TGTATGGGGAGGTTTCTGAT
Downstream 100 bases:
>100_bases GGAAGTGATGAAATGCCTGAATTACCAGAGGTTGAAAACGTCAGACGGACACTTGAAAATCTTGTAACAGGAAAAACAAT TGAAGACGTCATTGTTACCT
Product: DNA polymerase I
Products: NA
Alternate protein names: POL I [H]
Number of amino acids: Translated: 877; Mature: 877
Protein sequence:
>877_residues MEKKVVLVDGNNIAYRAFFALPLLNNDKGIHTNAIYGFTMMLMRILEEEKPTHMLVAFDAGKTTFRHKTYSEYKGGRQKT PPELSEQFPFIREMLDAFNVPRYELENYEADDIMGTLAKEASEQGASVKVISGDKDLLQLVSDNTLVCIPRKGITEVDEY TKEALFEKYSLSPKQIIDMKGLMGDQSDNIPGVPGVGEKTAIKLLTQFGTVEEVYENIDQVSGKKLKEKLEANKDQALMS KDLATIITDAPITVNVDDMEYKGYEASDVIPMFENLGFTSLLNKLGVTPEETAPAELDDITFDIVEEVTEEMLQQDSALI VEVQEDNYHKADIQGFGIQNENGCYFIQTDIALKSDAFKEWLADGEMRKYTFDAKRAIVALKWNGIDMQGIDFDLLIAAY LLDPADTDKDFRTVAKMKETHAVKSDEEVYGKGAKRAVPELEIVAEHVARKVHVLYDVKQTFVEELEKNEQYELFTELEL PLARVLADMEVKGVKVDTERLRNMGEELAGRLKEMEQEIYKLAGTEFNINSPKQLGVILFENLNLPVIKKTKTGYSTSAD VLDKLMDHHEIIPNILHYRQLGKLNSTYIEGLLKVVHEDSSKIHTRFNQVLTQTGRLSSTDPNLQNIPIRLEEGRKIRQA FVPSEEGWIMYAADYSQIELRVLAHIANDKGLVEAFQHDMDIHTKTAMDVFGVEKDEVTSNMRRQAKAVNFGIVYGISDY GLSQNLGITRKAAAEFIEKYLESFPGVQEYMDDIVKDAKQKGYVATLLNRRRYIPEITSRNFNLRSFAERTAMNTPIQGT AADIIKKAMIIMADRLEEEGLQARLLLQVHDELIFEAPKEEVEKLEKLVPEVMEHAIELAVPLKVDYSYGPTWYDAK
Sequences:
>Translated_877_residues MEKKVVLVDGNNIAYRAFFALPLLNNDKGIHTNAIYGFTMMLMRILEEEKPTHMLVAFDAGKTTFRHKTYSEYKGGRQKT PPELSEQFPFIREMLDAFNVPRYELENYEADDIMGTLAKEASEQGASVKVISGDKDLLQLVSDNTLVCIPRKGITEVDEY TKEALFEKYSLSPKQIIDMKGLMGDQSDNIPGVPGVGEKTAIKLLTQFGTVEEVYENIDQVSGKKLKEKLEANKDQALMS KDLATIITDAPITVNVDDMEYKGYEASDVIPMFENLGFTSLLNKLGVTPEETAPAELDDITFDIVEEVTEEMLQQDSALI VEVQEDNYHKADIQGFGIQNENGCYFIQTDIALKSDAFKEWLADGEMRKYTFDAKRAIVALKWNGIDMQGIDFDLLIAAY LLDPADTDKDFRTVAKMKETHAVKSDEEVYGKGAKRAVPELEIVAEHVARKVHVLYDVKQTFVEELEKNEQYELFTELEL PLARVLADMEVKGVKVDTERLRNMGEELAGRLKEMEQEIYKLAGTEFNINSPKQLGVILFENLNLPVIKKTKTGYSTSAD VLDKLMDHHEIIPNILHYRQLGKLNSTYIEGLLKVVHEDSSKIHTRFNQVLTQTGRLSSTDPNLQNIPIRLEEGRKIRQA FVPSEEGWIMYAADYSQIELRVLAHIANDKGLVEAFQHDMDIHTKTAMDVFGVEKDEVTSNMRRQAKAVNFGIVYGISDY GLSQNLGITRKAAAEFIEKYLESFPGVQEYMDDIVKDAKQKGYVATLLNRRRYIPEITSRNFNLRSFAERTAMNTPIQGT AADIIKKAMIIMADRLEEEGLQARLLLQVHDELIFEAPKEEVEKLEKLVPEVMEHAIELAVPLKVDYSYGPTWYDAK >Mature_877_residues MEKKVVLVDGNNIAYRAFFALPLLNNDKGIHTNAIYGFTMMLMRILEEEKPTHMLVAFDAGKTTFRHKTYSEYKGGRQKT PPELSEQFPFIREMLDAFNVPRYELENYEADDIMGTLAKEASEQGASVKVISGDKDLLQLVSDNTLVCIPRKGITEVDEY TKEALFEKYSLSPKQIIDMKGLMGDQSDNIPGVPGVGEKTAIKLLTQFGTVEEVYENIDQVSGKKLKEKLEANKDQALMS KDLATIITDAPITVNVDDMEYKGYEASDVIPMFENLGFTSLLNKLGVTPEETAPAELDDITFDIVEEVTEEMLQQDSALI VEVQEDNYHKADIQGFGIQNENGCYFIQTDIALKSDAFKEWLADGEMRKYTFDAKRAIVALKWNGIDMQGIDFDLLIAAY LLDPADTDKDFRTVAKMKETHAVKSDEEVYGKGAKRAVPELEIVAEHVARKVHVLYDVKQTFVEELEKNEQYELFTELEL PLARVLADMEVKGVKVDTERLRNMGEELAGRLKEMEQEIYKLAGTEFNINSPKQLGVILFENLNLPVIKKTKTGYSTSAD VLDKLMDHHEIIPNILHYRQLGKLNSTYIEGLLKVVHEDSSKIHTRFNQVLTQTGRLSSTDPNLQNIPIRLEEGRKIRQA FVPSEEGWIMYAADYSQIELRVLAHIANDKGLVEAFQHDMDIHTKTAMDVFGVEKDEVTSNMRRQAKAVNFGIVYGISDY GLSQNLGITRKAAAEFIEKYLESFPGVQEYMDDIVKDAKQKGYVATLLNRRRYIPEITSRNFNLRSFAERTAMNTPIQGT AADIIKKAMIIMADRLEEEGLQARLLLQVHDELIFEAPKEEVEKLEKLVPEVMEHAIELAVPLKVDYSYGPTWYDAK
Specific function: In addition to polymerase activity, this DNA polymerase exhibits 3' to 5' and 5' to 3' exonuclease activity [H]
COG id: COG0749
COG function: function code L; DNA polymerase I - 3'-5' exonuclease and polymerase domains
Gene ontology:
Cell location: Cytoplasm [C]
Metaboloic importance: Essential [C]
Operon status: Not Known
Operon components: None
Similarity: Contains 1 5'-3' exonuclease domain [H]
Homologues:
Organism=Homo sapiens, GI112420958, Length=516, Percent_Identity=34.1085271317829, Blast_Score=266, Evalue=5e-71, Organism=Homo sapiens, GI139394648, Length=634, Percent_Identity=26.813880126183, Blast_Score=213, Evalue=5e-55, Organism=Escherichia coli, GI1790294, Length=925, Percent_Identity=37.8378378378378, Blast_Score=561, Evalue=1e-161, Organism=Escherichia coli, GI226510970, Length=225, Percent_Identity=32, Blast_Score=124, Evalue=2e-29, Organism=Caenorhabditis elegans, GI86563272, Length=410, Percent_Identity=28.780487804878, Blast_Score=177, Evalue=2e-44, Organism=Drosophila melanogaster, GI17933644, Length=419, Percent_Identity=32.9355608591885, Blast_Score=236, Evalue=6e-62,
Paralogues:
None
Copy number: 400 Molecules/Cell In: Glucose minimal media [C]
Swissprot (AC and ID): NA
Other databases:
- InterPro: IPR002562 - InterPro: IPR020046 - InterPro: IPR020045 - InterPro: IPR002421 - InterPro: IPR020047 - InterPro: IPR019760 - InterPro: IPR001098 - InterPro: IPR018320 - InterPro: IPR002298 - InterPro: IPR008918 - InterPro: IPR003583 - InterPro: IPR012337 [H]
Pfam domain/function: PF01367 5_3_exonuc; PF02739 5_3_exonuc_N; PF00476 DNA_pol_A [H]
EC number: =2.7.7.7 [H]
Molecular weight: Translated: 99419; Mature: 99419
Theoretical pI: Translated: 4.64; Mature: 4.64
Prosite motif: PS00447 DNA_POLYMERASE_A
Important sites: NA
Signals:
None
Transmembrane regions:
None
Cys/Met content:
0.2 %Cys (Translated Protein) 3.3 %Met (Translated Protein) 3.5 %Cys+Met (Translated Protein) 0.2 %Cys (Mature Protein) 3.3 %Met (Mature Protein) 3.5 %Cys+Met (Mature Protein)
Secondary structure:
>Translated Secondary Structure MEKKVVLVDGNNIAYRAFFALPLLNNDKGIHTNAIYGFTMMLMRILEEEKPTHMLVAFDA CCCEEEEEECCCEEEEEEEEEEEECCCCCCCCHHHHHHHHHHHHHHHCCCCCEEEEEEEC GKTTFRHKTYSEYKGGRQKTPPELSEQFPFIREMLDAFNVPRYELENYEADDIMGTLAKE CCCHHCCCCHHHHCCCCCCCCCCHHHCCHHHHHHHHHHCCCCHHHCCCCHHHHHHHHHHH ASEQGASVKVISGDKDLLQLVSDNTLVCIPRKGITEVDEYTKEALFEKYSLSPKQIIDMK HHHCCCEEEEECCCHHHHHHHCCCCEEEECCCCCHHHHHHHHHHHHHHHCCCHHHHHHHH GLMGDQSDNIPGVPGVGEKTAIKLLTQFGTVEEVYENIDQVSGKKLKEKLEANKDQALMS HCCCCCCCCCCCCCCCCHHHHHHHHHHHCCHHHHHHHHHHHCCHHHHHHHHCCHHHHHHH KDLATIITDAPITVNVDDMEYKGYEASDVIPMFENLGFTSLLNKLGVTPEETAPAELDDI HHHHHHHCCCCEEEEECCCCCCCCCCCCCHHHHHCCCHHHHHHHHCCCCCCCCCCCHHHH TFDIVEEVTEEMLQQDSALIVEVQEDNYHKADIQGFGIQNENGCYFIQTDIALKSDAFKE HHHHHHHHHHHHHCCCCEEEEEEECCCCCCCCCEECCCCCCCCEEEEEECCCHHHHHHHH WLADGEMRKYTFDAKRAIVALKWNGIDMQGIDFDLLIAAYLLDPADTDKDFRTVAKMKET HHCCCCHHEEEECCCEEEEEEEECCCCCCCCCHHHHHHHHHCCCCCCCHHHHHHHHHHHH HAVKSDEEVYGKGAKRAVPELEIVAEHVARKVHVLYDVKQTFVEELEKNEQYELFTELEL HCCCCCHHHHCCCHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEHHHHHH PLARVLADMEVKGVKVDTERLRNMGEELAGRLKEMEQEIYKLAGTEFNINSPKQLGVILF HHHHHHHCCCCCCEEECHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCHHCCEEEE ENLNLPVIKKTKTGYSTSADVLDKLMDHHEIIPNILHYRQLGKLNSTYIEGLLKVVHEDS ECCCCCEEEECCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHCCC SKIHTRFNQVLTQTGRLSSTDPNLQNIPIRLEEGRKIRQAFVPSEEGWIMYAADYSQIEL HHHHHHHHHHHHHCCCCCCCCCCCCCCCEEECCCCHHHHHCCCCCCCEEEEECCCCHHHH RVLAHIANDKGLVEAFQHDMDIHTKTAMDVFGVEKDEVTSNMRRQAKAVNFGIVYGISDY HHHHHHHCCCHHHHHHHHCCCCHHHHHHHHHCCCHHHHHHHHHHHHHHCCEEEEEEECCC GLSQNLGITRKAAAEFIEKYLESFPGVQEYMDDIVKDAKQKGYVATLLNRRRYIPEITSR CCCCCCCCHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHCCHHHHHHHHHHCCCHHHCC NFNLRSFAERTAMNTPIQGTAADIIKKAMIIMADRLEEEGLQARLLLQVHDELIFEAPKE CCCHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHCCCHH EVEKLEKLVPEVMEHAIELAVPLKVDYSYGPTWYDAK HHHHHHHHHHHHHHHHHHEEEEEEEECCCCCEECCCC >Mature Secondary Structure MEKKVVLVDGNNIAYRAFFALPLLNNDKGIHTNAIYGFTMMLMRILEEEKPTHMLVAFDA CCCEEEEEECCCEEEEEEEEEEEECCCCCCCCHHHHHHHHHHHHHHHCCCCCEEEEEEEC GKTTFRHKTYSEYKGGRQKTPPELSEQFPFIREMLDAFNVPRYELENYEADDIMGTLAKE CCCHHCCCCHHHHCCCCCCCCCCHHHCCHHHHHHHHHHCCCCHHHCCCCHHHHHHHHHHH ASEQGASVKVISGDKDLLQLVSDNTLVCIPRKGITEVDEYTKEALFEKYSLSPKQIIDMK HHHCCCEEEEECCCHHHHHHHCCCCEEEECCCCCHHHHHHHHHHHHHHHCCCHHHHHHHH GLMGDQSDNIPGVPGVGEKTAIKLLTQFGTVEEVYENIDQVSGKKLKEKLEANKDQALMS HCCCCCCCCCCCCCCCCHHHHHHHHHHHCCHHHHHHHHHHHCCHHHHHHHHCCHHHHHHH KDLATIITDAPITVNVDDMEYKGYEASDVIPMFENLGFTSLLNKLGVTPEETAPAELDDI HHHHHHHCCCCEEEEECCCCCCCCCCCCCHHHHHCCCHHHHHHHHCCCCCCCCCCCHHHH TFDIVEEVTEEMLQQDSALIVEVQEDNYHKADIQGFGIQNENGCYFIQTDIALKSDAFKE HHHHHHHHHHHHHCCCCEEEEEEECCCCCCCCCEECCCCCCCCEEEEEECCCHHHHHHHH WLADGEMRKYTFDAKRAIVALKWNGIDMQGIDFDLLIAAYLLDPADTDKDFRTVAKMKET HHCCCCHHEEEECCCEEEEEEEECCCCCCCCCHHHHHHHHHCCCCCCCHHHHHHHHHHHH HAVKSDEEVYGKGAKRAVPELEIVAEHVARKVHVLYDVKQTFVEELEKNEQYELFTELEL HCCCCCHHHHCCCHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEHHHHHH PLARVLADMEVKGVKVDTERLRNMGEELAGRLKEMEQEIYKLAGTEFNINSPKQLGVILF HHHHHHHCCCCCCEEECHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCHHCCEEEE ENLNLPVIKKTKTGYSTSADVLDKLMDHHEIIPNILHYRQLGKLNSTYIEGLLKVVHEDS ECCCCCEEEECCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHCCC SKIHTRFNQVLTQTGRLSSTDPNLQNIPIRLEEGRKIRQAFVPSEEGWIMYAADYSQIEL HHHHHHHHHHHHHCCCCCCCCCCCCCCCEEECCCCHHHHHCCCCCCCEEEEECCCCHHHH RVLAHIANDKGLVEAFQHDMDIHTKTAMDVFGVEKDEVTSNMRRQAKAVNFGIVYGISDY HHHHHHHCCCHHHHHHHHCCCCHHHHHHHHHCCCHHHHHHHHHHHHHHCCEEEEEEECCC GLSQNLGITRKAAAEFIEKYLESFPGVQEYMDDIVKDAKQKGYVATLLNRRRYIPEITSR CCCCCCCCHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHCCHHHHHHHHHHCCCHHHCC NFNLRSFAERTAMNTPIQGTAADIIKKAMIIMADRLEEEGLQARLLLQVHDELIFEAPKE CCCHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHCCCHH EVEKLEKLVPEVMEHAIELAVPLKVDYSYGPTWYDAK HHHHHHHHHHHHHHHHHHEEEEEEEECCCCCEECCCC
PDB accession: NA
Resolution: NA
Structure class: Unstructured
Cofactors: NA
Metal ions: NA
Kcat value (1/min): NA
Specific activity: NA
Km value (mM): NA
Substrates: NA
Specific reaction: NA
General reaction: NA
Inhibitor: NA
Structure determination priority: 9.0
TargetDB status: NA
Availability: NA
References: 9387221; 9384377 [H]