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The map label for this gene is merA [H]

Identifier: 218905648

GI number: 218905648

Start: 4290613

End: 4292253

Strand: Reverse

Name: merA [H]

Synonym: BCAH820_4533

Alternate gene names: 218905648

Gene position: 4292253-4290613 (Counterclockwise)

Preceding gene: 218905649

Following gene: 218905647

Centisome position: 80.94

GC content: 41.99

Gene sequence:

>1641_bases
ATGAAAAAGTATCGAGTAAACGTTCAAGGGATGACTTGCACGGGTTGTGAAGAGCATGTTGCTGTTGCTCTTGAAAACAT
GGGTGCAAAAGAGATTGAAGTGGATTTTCGTCGCGAAGAAGCCGTATTTGAGCTTCCGAATGACGTGGAGGTGGAAAGAG
CGAAAAAGGCGATTTCTGAAGCCAAATACCAGCCAGGAGAAACAGAAGAAGTAAACCAGCAAGAAACGGTGCAACTTGGA
GATGAAGGCGATTATGACTACATTATCATTGGTTCTGGTGGAGCATCCTTTTCATCTGCCATTGAAGCCGCGAAATACGG
AGCGAACGTGGCAATCATCGAACGTGGCACAGTGGGTGGAACATGCGTGAACATCGGATGTGTTCCTTCAAAGACGCTGT
TAAGAGCTGGAGAAATCAATCACATAGCAAAAAACAATCCGTTTGTGGGATTGCACACGTCAGCAGGCAATGTCGATTTG
GCTCCATTGATTAAGCAGAAAAACGATTTGGTGACGGATCTTCGAAACTCGAAATATGTTGATTTAATTGATGACTACGG
CTTTGAATTAATAAAAGGTGAAGCGAAATTCGTGGATGAAAAAACCGTCGAAGTGAATGGCAAGCAGTTATCAGCCAAAC
GATTTTTAATCGCGACAGGTGCTTCACCTGCTGTACCAAAGATTCCTGGATTAGAAGAGGTAGATTTTTTAACAAGTACA
ACATTACTTGAATTAAAACAGGTTCCAAAGCGTCTTACAGTCATCGGTTCAGGATATATCGGTATGGAATTGGGACAACT
ATTCCATCATTTGGGTTCAGAAGTAACATTGATGCAAAGAAGTCCACGACTGTTAAAGGAATACGATCCTGAAGTGTCAG
AAGCGATCACGCAAGCATTAACAGAACAAGGGATTAATTTAGTAACAGGAGCATCCTTTGAACGAATCGAACAAGACGGA
GACGTTAAAAAGGTTCATGTTGAAGTCAATGGCAAGAAGCGAATCATTGAAGCAGATCAATTACTAGTTGCCACTGGAAG
AACACCAAATACGACGACTTTGGATTTAGAGGTAGCAGGTGTTGAAGTAGGCTCCCGTGGTGAAATTATAATCGATGAGT
ATTCAAAAACAACGAATTCAAGCATCTATGCAGCAGGAGATGTGACGCTTGGACCTCAATTTGTATATGTGGCAGCTTAT
CAAGGTGGAATTGCAGCAGGAAATGCCATCGGAGGACTGAATAAAAAGCTGAATTTAGAAGTGGTTCCAGGGGTTACGTT
TACAGCTCCAGCGATTGCAACCGTTGGTTTAACGGAGCAACAAGCAAAAGAAAAAGGCTATGAAGTGAAAACATCCGTCT
TGCCTTTAGATGCCGTTCCGAGAGCCTTGGTTAATCGAGAAACAACAGGTGTATATAAATTAGTGGCAGACGCAAAAACA
ATGAAGATATTAGGGGCTCATGTAGTAGCAGAAAATGCAGGAGAAGTAATTTATGCGGCGACACTGGCTATCAAATTCGG
TTTAACCGTGGAAGATCTCCGAGAAACCATGGCTCCATATTTAACAATGGCAGAAGGTATAAAATTAGCTGCCCTTACGT
TTGATAAAGATGTATCAAAATTATCTTGTTGTGCAGGTTAA

Upstream 100 bases:

>100_bases
GTAACGGTTTCCTTTGATGATAGGAAAACGGATTTACAGGTAATTAAGACGGCAATTTCGGATTTCGGTCTTGGAGTCGA
GTAAAAGGAGGAATATTAAT

Downstream 100 bases:

>100_bases
TCACCTTTCCAGTAAACTTTGATTTACTCCTTAGTTAATGAAAATTTTTATAGAAGACGATTCTTCTCTTAGCGTAGAGA
CAATCGTCTTTTTTTCTTCT

Product: mercuric reductase

Products: NA

Alternate protein names: Hg(II) reductase [H]

Number of amino acids: Translated: 546; Mature: 546

Protein sequence:

>546_residues
MKKYRVNVQGMTCTGCEEHVAVALENMGAKEIEVDFRREEAVFELPNDVEVERAKKAISEAKYQPGETEEVNQQETVQLG
DEGDYDYIIIGSGGASFSSAIEAAKYGANVAIIERGTVGGTCVNIGCVPSKTLLRAGEINHIAKNNPFVGLHTSAGNVDL
APLIKQKNDLVTDLRNSKYVDLIDDYGFELIKGEAKFVDEKTVEVNGKQLSAKRFLIATGASPAVPKIPGLEEVDFLTST
TLLELKQVPKRLTVIGSGYIGMELGQLFHHLGSEVTLMQRSPRLLKEYDPEVSEAITQALTEQGINLVTGASFERIEQDG
DVKKVHVEVNGKKRIIEADQLLVATGRTPNTTTLDLEVAGVEVGSRGEIIIDEYSKTTNSSIYAAGDVTLGPQFVYVAAY
QGGIAAGNAIGGLNKKLNLEVVPGVTFTAPAIATVGLTEQQAKEKGYEVKTSVLPLDAVPRALVNRETTGVYKLVADAKT
MKILGAHVVAENAGEVIYAATLAIKFGLTVEDLRETMAPYLTMAEGIKLAALTFDKDVSKLSCCAG

Sequences:

>Translated_546_residues
MKKYRVNVQGMTCTGCEEHVAVALENMGAKEIEVDFRREEAVFELPNDVEVERAKKAISEAKYQPGETEEVNQQETVQLG
DEGDYDYIIIGSGGASFSSAIEAAKYGANVAIIERGTVGGTCVNIGCVPSKTLLRAGEINHIAKNNPFVGLHTSAGNVDL
APLIKQKNDLVTDLRNSKYVDLIDDYGFELIKGEAKFVDEKTVEVNGKQLSAKRFLIATGASPAVPKIPGLEEVDFLTST
TLLELKQVPKRLTVIGSGYIGMELGQLFHHLGSEVTLMQRSPRLLKEYDPEVSEAITQALTEQGINLVTGASFERIEQDG
DVKKVHVEVNGKKRIIEADQLLVATGRTPNTTTLDLEVAGVEVGSRGEIIIDEYSKTTNSSIYAAGDVTLGPQFVYVAAY
QGGIAAGNAIGGLNKKLNLEVVPGVTFTAPAIATVGLTEQQAKEKGYEVKTSVLPLDAVPRALVNRETTGVYKLVADAKT
MKILGAHVVAENAGEVIYAATLAIKFGLTVEDLRETMAPYLTMAEGIKLAALTFDKDVSKLSCCAG
>Mature_546_residues
MKKYRVNVQGMTCTGCEEHVAVALENMGAKEIEVDFRREEAVFELPNDVEVERAKKAISEAKYQPGETEEVNQQETVQLG
DEGDYDYIIIGSGGASFSSAIEAAKYGANVAIIERGTVGGTCVNIGCVPSKTLLRAGEINHIAKNNPFVGLHTSAGNVDL
APLIKQKNDLVTDLRNSKYVDLIDDYGFELIKGEAKFVDEKTVEVNGKQLSAKRFLIATGASPAVPKIPGLEEVDFLTST
TLLELKQVPKRLTVIGSGYIGMELGQLFHHLGSEVTLMQRSPRLLKEYDPEVSEAITQALTEQGINLVTGASFERIEQDG
DVKKVHVEVNGKKRIIEADQLLVATGRTPNTTTLDLEVAGVEVGSRGEIIIDEYSKTTNSSIYAAGDVTLGPQFVYVAAY
QGGIAAGNAIGGLNKKLNLEVVPGVTFTAPAIATVGLTEQQAKEKGYEVKTSVLPLDAVPRALVNRETTGVYKLVADAKT
MKILGAHVVAENAGEVIYAATLAIKFGLTVEDLRETMAPYLTMAEGIKLAALTFDKDVSKLSCCAG

Specific function: Resistance to Hg(2+) in bacteria appears to be governed by a specialized system which includes mercuric reductase. MerA protein is responsible for volatilizing mercury as Hg(0) [H]

COG id: COG1249

COG function: function code C; Pyruvate/2-oxoglutarate dehydrogenase complex, dihydrolipoamide dehydrogenase (E3) component, and related enzymes

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 2 HMA domains [H]

Homologues:

Organism=Homo sapiens, GI91199540, Length=475, Percent_Identity=33.4736842105263, Blast_Score=228, Evalue=1e-59,
Organism=Homo sapiens, GI50301238, Length=460, Percent_Identity=32.3913043478261, Blast_Score=217, Evalue=2e-56,
Organism=Homo sapiens, GI22035672, Length=468, Percent_Identity=29.0598290598291, Blast_Score=156, Evalue=5e-38,
Organism=Homo sapiens, GI148277065, Length=480, Percent_Identity=28.9583333333333, Blast_Score=141, Evalue=2e-33,
Organism=Homo sapiens, GI33519430, Length=484, Percent_Identity=29.3388429752066, Blast_Score=140, Evalue=2e-33,
Organism=Homo sapiens, GI33519428, Length=484, Percent_Identity=29.3388429752066, Blast_Score=140, Evalue=2e-33,
Organism=Homo sapiens, GI33519426, Length=484, Percent_Identity=29.3388429752066, Blast_Score=140, Evalue=2e-33,
Organism=Homo sapiens, GI148277071, Length=481, Percent_Identity=29.1060291060291, Blast_Score=140, Evalue=2e-33,
Organism=Homo sapiens, GI291045266, Length=493, Percent_Identity=26.7748478701826, Blast_Score=128, Evalue=1e-29,
Organism=Homo sapiens, GI291045268, Length=488, Percent_Identity=25.4098360655738, Blast_Score=106, Evalue=6e-23,
Organism=Escherichia coli, GI87081717, Length=452, Percent_Identity=33.6283185840708, Blast_Score=243, Evalue=2e-65,
Organism=Escherichia coli, GI1786307, Length=463, Percent_Identity=31.9654427645788, Blast_Score=231, Evalue=1e-61,
Organism=Escherichia coli, GI1789915, Length=450, Percent_Identity=34, Blast_Score=216, Evalue=2e-57,
Organism=Escherichia coli, GI87082354, Length=466, Percent_Identity=31.7596566523605, Blast_Score=200, Evalue=2e-52,
Organism=Escherichia coli, GI1789065, Length=193, Percent_Identity=31.0880829015544, Blast_Score=80, Evalue=2e-16,
Organism=Caenorhabditis elegans, GI32565766, Length=463, Percent_Identity=34.1252699784017, Blast_Score=236, Evalue=3e-62,
Organism=Caenorhabditis elegans, GI17557007, Length=476, Percent_Identity=30.0420168067227, Blast_Score=182, Evalue=5e-46,
Organism=Caenorhabditis elegans, GI71983429, Length=455, Percent_Identity=31.4285714285714, Blast_Score=176, Evalue=3e-44,
Organism=Caenorhabditis elegans, GI71983419, Length=455, Percent_Identity=31.4285714285714, Blast_Score=176, Evalue=4e-44,
Organism=Caenorhabditis elegans, GI71982272, Length=498, Percent_Identity=27.9116465863454, Blast_Score=143, Evalue=2e-34,
Organism=Caenorhabditis elegans, GI17559934, Length=262, Percent_Identity=27.0992366412214, Blast_Score=78, Evalue=1e-14,
Organism=Saccharomyces cerevisiae, GI6321091, Length=483, Percent_Identity=35.1966873706004, Blast_Score=237, Evalue=3e-63,
Organism=Saccharomyces cerevisiae, GI6325166, Length=466, Percent_Identity=31.5450643776824, Blast_Score=209, Evalue=9e-55,
Organism=Saccharomyces cerevisiae, GI6325240, Length=469, Percent_Identity=28.1449893390192, Blast_Score=153, Evalue=9e-38,
Organism=Drosophila melanogaster, GI21358499, Length=469, Percent_Identity=35.181236673774, Blast_Score=231, Evalue=7e-61,
Organism=Drosophila melanogaster, GI24640549, Length=488, Percent_Identity=30.7377049180328, Blast_Score=170, Evalue=2e-42,
Organism=Drosophila melanogaster, GI24640551, Length=499, Percent_Identity=30.4609218436874, Blast_Score=170, Evalue=2e-42,
Organism=Drosophila melanogaster, GI24640553, Length=488, Percent_Identity=30.7377049180328, Blast_Score=170, Evalue=3e-42,
Organism=Drosophila melanogaster, GI17737741, Length=499, Percent_Identity=28.2565130260521, Blast_Score=167, Evalue=2e-41,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR016156
- InterPro:   IPR013027
- InterPro:   IPR017969
- InterPro:   IPR006121
- InterPro:   IPR000815
- InterPro:   IPR021179
- InterPro:   IPR004099
- InterPro:   IPR012999
- InterPro:   IPR001327 [H]

Pfam domain/function: PF00403 HMA; PF00070 Pyr_redox; PF07992 Pyr_redox_2; PF02852 Pyr_redox_dim [H]

EC number: =1.16.1.1 [H]

Molecular weight: Translated: 58602; Mature: 58602

Theoretical pI: Translated: 4.76; Mature: 4.76

Prosite motif: PS00076 PYRIDINE_REDOX_1 ; PS01047 HMA_1 ; PS50846 HMA_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
1.5 %Met     (Translated Protein)
2.6 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
1.5 %Met     (Mature Protein)
2.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKKYRVNVQGMTCTGCEEHVAVALENMGAKEIEVDFRREEAVFELPNDVEVERAKKAISE
CCEEEEEEECEEECCCHHHHHHHHHHCCCCEEEEEECCCCCEEECCCCCHHHHHHHHHHH
AKYQPGETEEVNQQETVQLGDEGDYDYIIIGSGGASFSSAIEAAKYGANVAIIERGTVGG
HCCCCCCCCCCCCCCEEEECCCCCCCEEEEECCCCCHHHHHHHHHCCCCEEEEECCCCCC
TCVNIGCVPSKTLLRAGEINHIAKNNPFVGLHTSAGNVDLAPLIKQKNDLVTDLRNSKYV
EEEEECCCCCHHHHHCCCCCCHHCCCCEEEEECCCCCEEHHHHHHCCCHHHHHHCCCCEE
DLIDDYGFELIKGEAKFVDEKTVEVNGKQLSAKRFLIATGASPAVPKIPGLEEVDFLTST
EEHHHCCCEEEECCHHCCCCEEEEECCCEEECEEEEEEECCCCCCCCCCCCCHHHHHHHH
TLLELKQVPKRLTVIGSGYIGMELGQLFHHLGSEVTLMQRSPRLLKEYDPEVSEAITQAL
HHHHHHHCCCEEEEEECCCHHHHHHHHHHHCCCCEEEECCCCHHHHHCCCHHHHHHHHHH
TEQGINLVTGASFERIEQDGDVKKVHVEVNGKKRIIEADQLLVATGRTPNTTTLDLEVAG
HHCCCEEEECCCHHHHHCCCCEEEEEEEECCCEEEEECCEEEEEECCCCCCEEEEEEEEE
VEVGSRGEIIIDEYSKTTNSSIYAAGDVTLGPQFVYVAAYQGGIAAGNAIGGLNKKLNLE
EEECCCCCEEEECCCCCCCCEEEEECCEEECCCEEEEEEECCCCCCCCCCCCCCCEEEEE
VVPGVTFTAPAIATVGLTEQQAKEKGYEVKTSVLPLDAVPRALVNRETTGVYKLVADAKT
EECCCEECCCHHEEECCCHHHHHHCCCEEEEEEECHHHHHHHHHCCCCCCEEEEECCCHH
MKILGAHVVAENAGEVIYAATLAIKFGLTVEDLRETMAPYLTMAEGIKLAALTFDKDVSK
HHHHHHHEEECCCCCEEEEEEEEEEECCCHHHHHHHHHHHHHHHCCCEEEEEEECCCCHH
LSCCAG
HHHCCC
>Mature Secondary Structure
MKKYRVNVQGMTCTGCEEHVAVALENMGAKEIEVDFRREEAVFELPNDVEVERAKKAISE
CCEEEEEEECEEECCCHHHHHHHHHHCCCCEEEEEECCCCCEEECCCCCHHHHHHHHHHH
AKYQPGETEEVNQQETVQLGDEGDYDYIIIGSGGASFSSAIEAAKYGANVAIIERGTVGG
HCCCCCCCCCCCCCCEEEECCCCCCCEEEEECCCCCHHHHHHHHHCCCCEEEEECCCCCC
TCVNIGCVPSKTLLRAGEINHIAKNNPFVGLHTSAGNVDLAPLIKQKNDLVTDLRNSKYV
EEEEECCCCCHHHHHCCCCCCHHCCCCEEEEECCCCCEEHHHHHHCCCHHHHHHCCCCEE
DLIDDYGFELIKGEAKFVDEKTVEVNGKQLSAKRFLIATGASPAVPKIPGLEEVDFLTST
EEHHHCCCEEEECCHHCCCCEEEEECCCEEECEEEEEEECCCCCCCCCCCCCHHHHHHHH
TLLELKQVPKRLTVIGSGYIGMELGQLFHHLGSEVTLMQRSPRLLKEYDPEVSEAITQAL
HHHHHHHCCCEEEEEECCCHHHHHHHHHHHCCCCEEEECCCCHHHHHCCCHHHHHHHHHH
TEQGINLVTGASFERIEQDGDVKKVHVEVNGKKRIIEADQLLVATGRTPNTTTLDLEVAG
HHCCCEEEECCCHHHHHCCCCEEEEEEEECCCEEEEECCEEEEEECCCCCCEEEEEEEEE
VEVGSRGEIIIDEYSKTTNSSIYAAGDVTLGPQFVYVAAYQGGIAAGNAIGGLNKKLNLE
EEECCCCCEEEECCCCCCCCEEEEECCEEECCCEEEEEEECCCCCCCCCCCCCCCEEEEE
VVPGVTFTAPAIATVGLTEQQAKEKGYEVKTSVLPLDAVPRALVNRETTGVYKLVADAKT
EECCCEECCCHHEEECCCHHHHHHCCCEEEEEEECHHHHHHHHHCCCCCCEEEEECCCHH
MKILGAHVVAENAGEVIYAATLAIKFGLTVEDLRETMAPYLTMAEGIKLAALTFDKDVSK
HHHHHHHEEECCCCCEEEEEEEEEEECCCHHHHHHHHHHHHHHHCCCEEEEEEECCCCHH
LSCCAG
HHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 2536669; 10559175; 2067577 [H]