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The map label for this gene is ruvA [H]

Identifier: 218905615

GI number: 218905615

Start: 4257383

End: 4259008

Strand: Reverse

Name: ruvA [H]

Synonym: BCAH820_4500

Alternate gene names: 218905615

Gene position: 4259008-4257383 (Counterclockwise)

Preceding gene: 218905617

Following gene: 218905614

Centisome position: 80.32

GC content: 40.41

Gene sequence:

>1626_bases
TTGTTTGAATATGTTACAGGTTACGTGGAGTATGTAGGACCGGAATATGTCGTAATTGATCATAATGGAATTGGTTATCA
AATTTTCACACCGAATCCGTATGTATTTCAAAGAAGTAAGCAAGAAATCCGTGTCTATACATATCATTATGTGAGAGAAG
ATATTATGGCACTTTACGGGTTTAAAACACGTGAAGAGCGTTTATTATTTACAAAGTTGTTAGGGGTGTCTGGTATTGGA
CCAAAAGGTGCTCTTGCAATTTTAGCTTCTGGTCAAACAGGACAGGTCGTTCAAGCGATTGAACATGAAGACGAGAAGTT
TTTAGTGAAGTTCCCGGGCGTCGGAAAGAAAACAGCACGCCAAATGATTTTAGATTTAAAAGGAAAACTAGCAGACGTTG
TACCAGATGCGTTTGTTGATTTATTCTCAGATGAAGAACGTTTTGATGAGAAGAAAGGTTCGTCAGCTGAGCTTGATGAG
GCGCTGGAAGCTCTGCGCGCACTTGGATACGCAGAACGAGAAGTTTCTCGCGTTGTACCAGAGTTATTAAAAGAATCATT
AACGACGGATCAGTATATTAAAAAGGCACTTAGTCTTTTACTAAATGGTAAAGAGGTGAGTATAATGGACGAACGTCTCC
TTTCAGGGGAATCTGCATATGAAGATGCGGATTTAGAATATTCATTACGGCCACAGACGCTCCGTCAGTATATTGGCCAA
GATAAAGCGAAACATAATTTAGAAGTGTTTATTGAAGCGGCGAAAATGCGTGAAGAAACGTTAGATCACGTGCTTTTATA
TGGACCACCAGGACTTGGTAAAACGACGCTTGCAAATATTATTGCCAATGAAATGGGTGTAAATGTTAGAACAACTTCAG
GTCCAGCAATCGAAAGGCCAGGAGATTTAGCAGCTGTATTAACATCGCTTCAACCAGGGGATGTATTATTTATTGATGAA
ATTCATCGTTTGCATAGATCAATTGAAGAAGTACTATATCCTGCGATGGAAGATTTTTGCCTTGATATTGTCATTGGAAA
AGGACCGTCAGCGCGATCTGTACGTTTAGATTTACCGCCATTTACATTAGTTGGAGCAACGACGCGTGCGGGAGCATTAT
CAGCGCCATTACGTGACCGTTTCGGTGTACTTTCAAGATTAGAGTATTACACAGTAGATCAGCTTTCTGCGATTGTGGAA
CGTACAGCAGAAGTATTTGAAGTTGAAATTGATTCGTTAGCTGCACTAGAAATTGCAAGACGTGCTCGTGGTACACCTCG
TATTGCGAATCGTTTATTACGACGTGTACGAGATTTCGCACAAGTTCGTGGTAACGGAACAGTTACGATGGAAATTACGC
AAATGGCATTAGAATTGCTGCAAGTAGATAAATTAGGTCTAGATCATATTGACCATAAATTATTGCTTGGTATTATTGAA
AAATTCCGCGGTGGCCCAGTTGGACTAGAAACGGTTTCGGCAACGATTGGAGAAGAATCTCATACGATTGAAGATGTGTA
TGAGCCATATTTATTACAAATTGGCTTTTTACAACGAACGCCAAGGGGCCGGATTGTAACGCCGCTTGCATATGAGCATT
TCGGAATGGAGATGCCAAAAGTATGA

Upstream 100 bases:

>100_bases
CAGAAGTTTCAAATAGAGAACATTTGTTGGGGTAGTCTCGCTTTTCTATCATTAAATATGTTAAAATGGAATACATGATT
TAAGAGGGAGAGATCGTATT

Downstream 100 bases:

>100_bases
CGGAGATGCCGAAATTGCTTATTACAGCAGGTGTCTTACTTATCGTTGTTGGATTAGCTTGGAAGTTCGTTGGAAGGCTT
CCAGGCGATATTTTTGTGAA

Product: Holliday junction DNA helicase RuvA

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 541; Mature: 541

Protein sequence:

>541_residues
MFEYVTGYVEYVGPEYVVIDHNGIGYQIFTPNPYVFQRSKQEIRVYTYHYVREDIMALYGFKTREERLLFTKLLGVSGIG
PKGALAILASGQTGQVVQAIEHEDEKFLVKFPGVGKKTARQMILDLKGKLADVVPDAFVDLFSDEERFDEKKGSSAELDE
ALEALRALGYAEREVSRVVPELLKESLTTDQYIKKALSLLLNGKEVSIMDERLLSGESAYEDADLEYSLRPQTLRQYIGQ
DKAKHNLEVFIEAAKMREETLDHVLLYGPPGLGKTTLANIIANEMGVNVRTTSGPAIERPGDLAAVLTSLQPGDVLFIDE
IHRLHRSIEEVLYPAMEDFCLDIVIGKGPSARSVRLDLPPFTLVGATTRAGALSAPLRDRFGVLSRLEYYTVDQLSAIVE
RTAEVFEVEIDSLAALEIARRARGTPRIANRLLRRVRDFAQVRGNGTVTMEITQMALELLQVDKLGLDHIDHKLLLGIIE
KFRGGPVGLETVSATIGEESHTIEDVYEPYLLQIGFLQRTPRGRIVTPLAYEHFGMEMPKV

Sequences:

>Translated_541_residues
MFEYVTGYVEYVGPEYVVIDHNGIGYQIFTPNPYVFQRSKQEIRVYTYHYVREDIMALYGFKTREERLLFTKLLGVSGIG
PKGALAILASGQTGQVVQAIEHEDEKFLVKFPGVGKKTARQMILDLKGKLADVVPDAFVDLFSDEERFDEKKGSSAELDE
ALEALRALGYAEREVSRVVPELLKESLTTDQYIKKALSLLLNGKEVSIMDERLLSGESAYEDADLEYSLRPQTLRQYIGQ
DKAKHNLEVFIEAAKMREETLDHVLLYGPPGLGKTTLANIIANEMGVNVRTTSGPAIERPGDLAAVLTSLQPGDVLFIDE
IHRLHRSIEEVLYPAMEDFCLDIVIGKGPSARSVRLDLPPFTLVGATTRAGALSAPLRDRFGVLSRLEYYTVDQLSAIVE
RTAEVFEVEIDSLAALEIARRARGTPRIANRLLRRVRDFAQVRGNGTVTMEITQMALELLQVDKLGLDHIDHKLLLGIIE
KFRGGPVGLETVSATIGEESHTIEDVYEPYLLQIGFLQRTPRGRIVTPLAYEHFGMEMPKV
>Mature_541_residues
MFEYVTGYVEYVGPEYVVIDHNGIGYQIFTPNPYVFQRSKQEIRVYTYHYVREDIMALYGFKTREERLLFTKLLGVSGIG
PKGALAILASGQTGQVVQAIEHEDEKFLVKFPGVGKKTARQMILDLKGKLADVVPDAFVDLFSDEERFDEKKGSSAELDE
ALEALRALGYAEREVSRVVPELLKESLTTDQYIKKALSLLLNGKEVSIMDERLLSGESAYEDADLEYSLRPQTLRQYIGQ
DKAKHNLEVFIEAAKMREETLDHVLLYGPPGLGKTTLANIIANEMGVNVRTTSGPAIERPGDLAAVLTSLQPGDVLFIDE
IHRLHRSIEEVLYPAMEDFCLDIVIGKGPSARSVRLDLPPFTLVGATTRAGALSAPLRDRFGVLSRLEYYTVDQLSAIVE
RTAEVFEVEIDSLAALEIARRARGTPRIANRLLRRVRDFAQVRGNGTVTMEITQMALELLQVDKLGLDHIDHKLLLGIIE
KFRGGPVGLETVSATIGEESHTIEDVYEPYLLQIGFLQRTPRGRIVTPLAYEHFGMEMPKV

Specific function: The ruvA-ruvB complex in the presence of ATP renatures cruciform structure in supercoiled DNA with palindromic sequence, indicating that it may promote strand exchange reactions in homologous recombination. RuvAB is an helicase that mediates the Holliday

COG id: COG2255

COG function: function code L; Holliday junction resolvasome, helicase subunit

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the ruvB family [H]

Homologues:

Organism=Escherichia coli, GI1788167, Length=331, Percent_Identity=60.7250755287009, Blast_Score=430, Evalue=1e-121,
Organism=Escherichia coli, GI1788168, Length=190, Percent_Identity=37.3684210526316, Blast_Score=118, Evalue=8e-28,
Organism=Escherichia coli, GI1787119, Length=132, Percent_Identity=35.6060606060606, Blast_Score=63, Evalue=4e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003593
- InterPro:   IPR004605
- InterPro:   IPR008823
- InterPro:   IPR008824
- InterPro:   IPR011991
- ProDom:   PD005323 [H]

Pfam domain/function: PF05491 RuvB_C; PF05496 RuvB_N [H]

EC number: =3.6.4.12 [H]

Molecular weight: Translated: 60503; Mature: 60503

Theoretical pI: Translated: 5.04; Mature: 5.04

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.2 %Cys     (Translated Protein)
2.0 %Met     (Translated Protein)
2.2 %Cys+Met (Translated Protein)
0.2 %Cys     (Mature Protein)
2.0 %Met     (Mature Protein)
2.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MFEYVTGYVEYVGPEYVVIDHNGIGYQIFTPNPYVFQRSKQEIRVYTYHYVREDIMALYG
CCCHHHHHHHHCCCEEEEEECCCCEEEEECCCCCEECCCCCCEEEEHHHHHHHHHHHHHC
FKTREERLLFTKLLGVSGIGPKGALAILASGQTGQVVQAIEHEDEKFLVKFPGVGKKTAR
CCCHHHHHHHHHHHCCCCCCCCCCEEEEECCCCCHHHHHHHCCCCEEEEECCCCCHHHHH
QMILDLKGKLADVVPDAFVDLFSDEERFDEKKGSSAELDEALEALRALGYAEREVSRVVP
HHHHHHHHHHHHHHHHHHHHHHCCHHHHHHCCCCCCHHHHHHHHHHHHCHHHHHHHHHHH
ELLKESLTTDQYIKKALSLLLNGKEVSIMDERLLSGESAYEDADLEYSLRPQTLRQYIGQ
HHHHHHCCHHHHHHHHHHHHHCCCCCHHHHHHHHCCCCCCCCCCCEEECCHHHHHHHHCC
DKAKHNLEVFIEAAKMREETLDHVLLYGPPGLGKTTLANIIANEMGVNVRTTSGPAIERP
CCCCCCHHHHHHHHHHHHHHHCEEEEECCCCCCHHHHHHHHHHHHCCEEEECCCCCCCCC
GDLAAVLTSLQPGDVLFIDEIHRLHRSIEEVLYPAMEDFCLDIVIGKGPSARSVRLDLPP
CHHHHHHHCCCCCCEEEHHHHHHHHHHHHHHHHHHHHHHEEEEEECCCCCCCEEEECCCC
FTLVGATTRAGALSAPLRDRFGVLSRLEYYTVDQLSAIVERTAEVFEVEIDSLAALEIAR
EEEEECCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
RARGTPRIANRLLRRVRDFAQVRGNGTVTMEITQMALELLQVDKLGLDHIDHKLLLGIIE
HCCCCHHHHHHHHHHHHHHHHHCCCCEEEEHHHHHHHHHHHHHHCCHHHHHHHHHHHHHH
KFRGGPVGLETVSATIGEESHTIEDVYEPYLLQIGFLQRTPRGRIVTPLAYEHFGMEMPK
HHCCCCCCHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHCCCCCCEECCHHHHHHCCCCCC
V
C
>Mature Secondary Structure
MFEYVTGYVEYVGPEYVVIDHNGIGYQIFTPNPYVFQRSKQEIRVYTYHYVREDIMALYG
CCCHHHHHHHHCCCEEEEEECCCCEEEEECCCCCEECCCCCCEEEEHHHHHHHHHHHHHC
FKTREERLLFTKLLGVSGIGPKGALAILASGQTGQVVQAIEHEDEKFLVKFPGVGKKTAR
CCCHHHHHHHHHHHCCCCCCCCCCEEEEECCCCCHHHHHHHCCCCEEEEECCCCCHHHHH
QMILDLKGKLADVVPDAFVDLFSDEERFDEKKGSSAELDEALEALRALGYAEREVSRVVP
HHHHHHHHHHHHHHHHHHHHHHCCHHHHHHCCCCCCHHHHHHHHHHHHCHHHHHHHHHHH
ELLKESLTTDQYIKKALSLLLNGKEVSIMDERLLSGESAYEDADLEYSLRPQTLRQYIGQ
HHHHHHCCHHHHHHHHHHHHHCCCCCHHHHHHHHCCCCCCCCCCCEEECCHHHHHHHHCC
DKAKHNLEVFIEAAKMREETLDHVLLYGPPGLGKTTLANIIANEMGVNVRTTSGPAIERP
CCCCCCHHHHHHHHHHHHHHHCEEEEECCCCCCHHHHHHHHHHHHCCEEEECCCCCCCCC
GDLAAVLTSLQPGDVLFIDEIHRLHRSIEEVLYPAMEDFCLDIVIGKGPSARSVRLDLPP
CHHHHHHHCCCCCCEEEHHHHHHHHHHHHHHHHHHHHHHEEEEEECCCCCCCEEEECCCC
FTLVGATTRAGALSAPLRDRFGVLSRLEYYTVDQLSAIVERTAEVFEVEIDSLAALEIAR
EEEEECCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
RARGTPRIANRLLRRVRDFAQVRGNGTVTMEITQMALELLQVDKLGLDHIDHKLLLGIIE
HCCCCHHHHHHHHHHHHHHHHHCCCCEEEEHHHHHHHHHHHHHHCCHHHHHHHHHHHHHH
KFRGGPVGLETVSATIGEESHTIEDVYEPYLLQIGFLQRTPRGRIVTPLAYEHFGMEMPK
HHCCCCCCHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHCCCCCCEECCHHHHHHCCCCCC
V
C

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA