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The map label for this gene is ykuM [H]

Identifier: 218905113

GI number: 218905113

Start: 3831287

End: 3832171

Strand: Reverse

Name: ykuM [H]

Synonym: BCAH820_3997

Alternate gene names: 218905113

Gene position: 3832171-3831287 (Counterclockwise)

Preceding gene: 218905114

Following gene: 218905112

Centisome position: 72.27

GC content: 34.01

Gene sequence:

>885_bases
ATGGAAGCTAAGGAAAGAGGGAGAAAGTTGCAAATTGATGATTTTCAAATGATGGTTGTGTTAGCACAGGAATCAAATAT
GAGAAAAGCGGCGGAACGTTTATTTGTTTCACAACCTGCGCTTAGTCAACGATTACAATCTATGGAGAAACAATGGGGAA
TGAAGTTTTTTATTCGCTCGCAAAAAGGATTGACAATTACACCTGAAGGGGAAAAAGTTGCAAATTACGCAAAAGAAATG
CTGCAAAGGGAAGAGGATATTAAAAGTGAGCTAGCTGTTTTTAGAACAGAAACTCATGGGACGTTAAAAATAGCTGTCGC
ATCAGTTATTGGCCAATATTGGCTTCCTCCTGTTTTGAAGAAGTTTGTGCATAAATATCCTTCTGTGAAAATATCGTTGT
TTACTGGCTGGAGTAGTGAAGTGCAAAAGCAATTTTATGAAGGAGACGTTCATGTTGCTATATTGAGAGGAACACAAGAA
TATAAAGGCCAGAAGCAACAATTATTTGAAGATGAACTTTATTTAGTGGATAAAGAAATAAAAGATATTTCGATGTTAAA
AGAAACAAATAGACCATTTATTCAATTTAAAAGTGACTCGACTTATTATGGACAAATACAAAATTGGTGGTATGGTTTAT
TTTCTAATCCACCTAAGAGAACAATTGTCGTTGATCAAATTGAGACTTGTAAACAACTCGTTTTAAATGGCATAGGCTAC
GCGCTTTTACCTTCTACTGTTTTAAAAGAAGTACAAGAAAATATGTATAAAACACCTGTTCAATTAACGAGAGAAACATG
GTTATTGACGAGTGAATCAGCTAGACAATTGAAGCAAGTACAAGCATTTTTAGAAATTATAGAAGAAATTCAAATGGAAA
AATAG

Upstream 100 bases:

>100_bases
TTTCGTGCATATCAGTTGTGTATGTATTATATTTAAAAAGATAAAAAATATAAGAGTATGTAGTTAAATTAAGGATGAAA
AGCTTATTGAGATAAGGTGT

Downstream 100 bases:

>100_bases
GATTATTATCTTGCTACTCGCTCTATCGTTTGGTAGAGTAACATTATAAATGGATTTAGGAGGCAACAAGAATGAAAATG
ATGGACGCTAACGAAATTAT

Product: transcriptional regulator, LysR family

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 294; Mature: 294

Protein sequence:

>294_residues
MEAKERGRKLQIDDFQMMVVLAQESNMRKAAERLFVSQPALSQRLQSMEKQWGMKFFIRSQKGLTITPEGEKVANYAKEM
LQREEDIKSELAVFRTETHGTLKIAVASVIGQYWLPPVLKKFVHKYPSVKISLFTGWSSEVQKQFYEGDVHVAILRGTQE
YKGQKQQLFEDELYLVDKEIKDISMLKETNRPFIQFKSDSTYYGQIQNWWYGLFSNPPKRTIVVDQIETCKQLVLNGIGY
ALLPSTVLKEVQENMYKTPVQLTRETWLLTSESARQLKQVQAFLEIIEEIQMEK

Sequences:

>Translated_294_residues
MEAKERGRKLQIDDFQMMVVLAQESNMRKAAERLFVSQPALSQRLQSMEKQWGMKFFIRSQKGLTITPEGEKVANYAKEM
LQREEDIKSELAVFRTETHGTLKIAVASVIGQYWLPPVLKKFVHKYPSVKISLFTGWSSEVQKQFYEGDVHVAILRGTQE
YKGQKQQLFEDELYLVDKEIKDISMLKETNRPFIQFKSDSTYYGQIQNWWYGLFSNPPKRTIVVDQIETCKQLVLNGIGY
ALLPSTVLKEVQENMYKTPVQLTRETWLLTSESARQLKQVQAFLEIIEEIQMEK
>Mature_294_residues
MEAKERGRKLQIDDFQMMVVLAQESNMRKAAERLFVSQPALSQRLQSMEKQWGMKFFIRSQKGLTITPEGEKVANYAKEM
LQREEDIKSELAVFRTETHGTLKIAVASVIGQYWLPPVLKKFVHKYPSVKISLFTGWSSEVQKQFYEGDVHVAILRGTQE
YKGQKQQLFEDELYLVDKEIKDISMLKETNRPFIQFKSDSTYYGQIQNWWYGLFSNPPKRTIVVDQIETCKQLVLNGIGY
ALLPSTVLKEVQENMYKTPVQLTRETWLLTSESARQLKQVQAFLEIIEEIQMEK

Specific function: This Protein Is A Positive Regulator Of Gene Expression For The Cysteine Regulon, A System Of 10 Or More Loci Involved In The Biosynthesis Of L-Cysteine From Inorganic Sulfate. The Inducer For Cysb Is N-Acetylserine. Cysb Inhibits Its Own Transcription.

COG id: COG0583

COG function: function code K; Transcriptional regulator

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 HTH lysR-type DNA-binding domain [H]

Homologues:

Organism=Escherichia coli, GI1787530, Length=136, Percent_Identity=31.6176470588235, Blast_Score=81, Evalue=7e-17,
Organism=Escherichia coli, GI1787806, Length=292, Percent_Identity=20.2054794520548, Blast_Score=77, Evalue=2e-15,
Organism=Escherichia coli, GI1786401, Length=250, Percent_Identity=27.2, Blast_Score=73, Evalue=2e-14,
Organism=Escherichia coli, GI157672245, Length=115, Percent_Identity=33.0434782608696, Blast_Score=70, Evalue=1e-13,
Organism=Escherichia coli, GI1788296, Length=133, Percent_Identity=27.0676691729323, Blast_Score=70, Evalue=2e-13,
Organism=Escherichia coli, GI1790399, Length=244, Percent_Identity=22.5409836065574, Blast_Score=69, Evalue=3e-13,
Organism=Escherichia coli, GI1788706, Length=130, Percent_Identity=32.3076923076923, Blast_Score=69, Evalue=3e-13,
Organism=Escherichia coli, GI1790208, Length=253, Percent_Identity=23.7154150197628, Blast_Score=69, Evalue=4e-13,
Organism=Escherichia coli, GI1787601, Length=146, Percent_Identity=24.6575342465753, Blast_Score=69, Evalue=4e-13,
Organism=Escherichia coli, GI1788481, Length=230, Percent_Identity=26.0869565217391, Blast_Score=67, Evalue=1e-12,
Organism=Escherichia coli, GI1787589, Length=140, Percent_Identity=26.4285714285714, Blast_Score=67, Evalue=2e-12,
Organism=Escherichia coli, GI1787128, Length=148, Percent_Identity=23.6486486486486, Blast_Score=65, Evalue=4e-12,
Organism=Escherichia coli, GI145693105, Length=126, Percent_Identity=25.3968253968254, Blast_Score=65, Evalue=5e-12,
Organism=Escherichia coli, GI1788887, Length=247, Percent_Identity=24.2914979757085, Blast_Score=64, Evalue=2e-11,
Organism=Escherichia coli, GI1789506, Length=251, Percent_Identity=21.1155378486056, Blast_Score=63, Evalue=2e-11,
Organism=Escherichia coli, GI1789173, Length=152, Percent_Identity=26.3157894736842, Blast_Score=61, Evalue=7e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000847
- InterPro:   IPR005119
- InterPro:   IPR011991 [H]

Pfam domain/function: PF00126 HTH_1; PF03466 LysR_substrate [H]

EC number: NA

Molecular weight: Translated: 34308; Mature: 34308

Theoretical pI: Translated: 9.08; Mature: 9.08

Prosite motif: PS50931 HTH_LYSR

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.3 %Cys     (Translated Protein)
3.4 %Met     (Translated Protein)
3.7 %Cys+Met (Translated Protein)
0.3 %Cys     (Mature Protein)
3.4 %Met     (Mature Protein)
3.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MEAKERGRKLQIDDFQMMVVLAQESNMRKAAERLFVSQPALSQRLQSMEKQWGMKFFIRS
CCCHHCCCEECCCCCEEEEEEECCCCHHHHHHHHHHCCHHHHHHHHHHHHHCCCEEEEEC
QKGLTITPEGEKVANYAKEMLQREEDIKSELAVFRTETHGTLKIAVASVIGQYWLPPVLK
CCCEEECCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHCCCHHHH
KFVHKYPSVKISLFTGWSSEVQKQFYEGDVHVAILRGTQEYKGQKQQLFEDELYLVDKEI
HHHHHCCCEEEEEEECCCHHHHHHHHCCCEEEEEEECCHHHCCHHHHHHHHHHHHHHHHH
KDISMLKETNRPFIQFKSDSTYYGQIQNWWYGLFSNPPKRTIVVDQIETCKQLVLNGIGY
HHHHHHHHCCCCEEEECCCCCEEECHHHHHHHHCCCCCCCEEEHHHHHHHHHHHHHCCCH
ALLPSTVLKEVQENMYKTPVQLTRETWLLTSESARQLKQVQAFLEIIEEIQMEK
HHHHHHHHHHHHHHHHCCHHHHHHHHHEEECHHHHHHHHHHHHHHHHHHHHCCC
>Mature Secondary Structure
MEAKERGRKLQIDDFQMMVVLAQESNMRKAAERLFVSQPALSQRLQSMEKQWGMKFFIRS
CCCHHCCCEECCCCCEEEEEEECCCCHHHHHHHHHHCCHHHHHHHHHHHHHCCCEEEEEC
QKGLTITPEGEKVANYAKEMLQREEDIKSELAVFRTETHGTLKIAVASVIGQYWLPPVLK
CCCEEECCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHCCCHHHH
KFVHKYPSVKISLFTGWSSEVQKQFYEGDVHVAILRGTQEYKGQKQQLFEDELYLVDKEI
HHHHHCCCEEEEEEECCCHHHHHHHHCCCEEEEEEECCHHHCCHHHHHHHHHHHHHHHHH
KDISMLKETNRPFIQFKSDSTYYGQIQNWWYGLFSNPPKRTIVVDQIETCKQLVLNGIGY
HHHHHHHHCCCCEEEECCCCCEEECHHHHHHHHCCCCCCCEEEHHHHHHHHHHHHHCCCH
ALLPSTVLKEVQENMYKTPVQLTRETWLLTSESARQLKQVQAFLEIIEEIQMEK
HHHHHHHHHHHHHHHHCCHHHHHHHHHEEECHHHHHHHHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9384377 [H]