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The map label for this gene is pdhC [H]

Identifier: 218905101

GI number: 218905101

Start: 3819283

End: 3820572

Strand: Reverse

Name: pdhC [H]

Synonym: BCAH820_3985

Alternate gene names: 218905101

Gene position: 3820572-3819283 (Counterclockwise)

Preceding gene: 218905102

Following gene: 218905100

Centisome position: 72.05

GC content: 40.78

Gene sequence:

>1290_bases
GTGGCATTTGAATTTAAACTACCAGATATCGGTGAAGGTATCCACGAAGGTGAAATCGTAAAATGGTTTATTAAACCAGG
CGACGAAGTAAACGAAGACGACGTACTTCTTGAAGTACAAAATGATAAAGCAGTAGTAGAAATTCCTTCTCCTGTTAAAG
GTAAAGTACTTGAAGTACTTGTAGAAGAAGGTACGGTTGCAGTAGTTGGAGATACATTAATTAAATTTGATGCTCCAGGA
TACGAAAACCTTAAATTTAAAGGCGACGATCATGACGAAGCTCCTAAAGCTGAAGAAGCAAAAGAAGAAGCTCCTAAAGC
TGAAGCTACTCCAGCAGCAACTGCAGAAGTAGTAAATGAGCGCGTAATCGCTATGCCATCTGTTCGTAAATATGCTCGTG
AAAACGGCGTAGACATTCATAAAGTAGCTGGTTCTGGTAAGAACGGTCGTATCGTAAAAGCTGACATCGATGCATTTGCA
AATGGTGGACAAGCAGTAGCAGCAACTGAGGCTCCAGCAGCAGTAGAAGCTACTCCAGCAGCAGCGAAAGAAGAAGCACC
AAAAGCACAACCAATCCCAGCTGGTGAATATCCAGAAACTCGTGAGAAAATGAGTGGTATCCGTAAAGCAATTGCGAAAG
CAATGGTTAACTCTAAACATACAGCTCCTCACGTAACATTAATGGATGAAGTAGATGTAACTGAACTTGTTGCTCACCGT
AAGAAGTTCAAAGCTGTGGCAGCTGACAAAGGTATTAAATTAACTTACCTTCCATACGTTGTTAAAGCTTTAACATCTGC
ATTACGTGAATACCCAATGTTAAACACTTCTTTAGATGATGCTTCTCAAGAAGTAGTTCATAAACATTACTTCAACATCG
GTATCGCAGCTGATACAGACAAAGGTCTATTAGTACCAGTTGTTAAAGATACAGATCGCAAGTCTATCTTCACAATTTCT
AACGAGATCAATGATCTTGCTGGTAAAGCACGTGAAGGTCGTTTAGCTCCTGCTGAAATGAAAGGCGCTTCTTGCACAAT
TACAAACATTGGTTCTGCAGGTGGACAATGGTTCACTCCAGTTATCAACCACCCAGAAGTAGCAATCCTTGGTATCGGCC
GTATCGCTGAGAAACCAGTTGTGAAAAACGGTGAGATCGTTGCAGCTCCAGTATTAGCATTATCTCTAAGCTTTGACCAT
CGTTTAATTGACGGCGCAACTGCTCAAAAAGCATTAAACCAAATTAAACGTCTATTGAATGACCCACAATTATTAGTAAT
GGAGGCGTAA

Upstream 100 bases:

>100_bases
ACTTCTAATTTTTACTTATTCATGTGCAAAAGAAATCAGCACATGCTGATTTCTTTTGCCATATTCCATAATAAAGAAAT
GAATCAGGGGGCTGAATTCC

Downstream 100 bases:

>100_bases
TAACAATGGTAGTAGGAGATTTCCCAATTGAATTAGATACAGTCGTTGTTGGTGCAGGTCCTGGTGGATACGTTGCGGCA
ATTCGTGCAGCACAATTAGG

Product: branched-chain alpha-keto acid dehydrogenase subunit E2

Products: NA

Alternate protein names: Dihydrolipoamide acetyltransferase component of pyruvate dehydrogenase complex; E2; S complex, 48 kDa subunit [H]

Number of amino acids: Translated: 429; Mature: 428

Protein sequence:

>429_residues
MAFEFKLPDIGEGIHEGEIVKWFIKPGDEVNEDDVLLEVQNDKAVVEIPSPVKGKVLEVLVEEGTVAVVGDTLIKFDAPG
YENLKFKGDDHDEAPKAEEAKEEAPKAEATPAATAEVVNERVIAMPSVRKYARENGVDIHKVAGSGKNGRIVKADIDAFA
NGGQAVAATEAPAAVEATPAAAKEEAPKAQPIPAGEYPETREKMSGIRKAIAKAMVNSKHTAPHVTLMDEVDVTELVAHR
KKFKAVAADKGIKLTYLPYVVKALTSALREYPMLNTSLDDASQEVVHKHYFNIGIAADTDKGLLVPVVKDTDRKSIFTIS
NEINDLAGKAREGRLAPAEMKGASCTITNIGSAGGQWFTPVINHPEVAILGIGRIAEKPVVKNGEIVAAPVLALSLSFDH
RLIDGATAQKALNQIKRLLNDPQLLVMEA

Sequences:

>Translated_429_residues
MAFEFKLPDIGEGIHEGEIVKWFIKPGDEVNEDDVLLEVQNDKAVVEIPSPVKGKVLEVLVEEGTVAVVGDTLIKFDAPG
YENLKFKGDDHDEAPKAEEAKEEAPKAEATPAATAEVVNERVIAMPSVRKYARENGVDIHKVAGSGKNGRIVKADIDAFA
NGGQAVAATEAPAAVEATPAAAKEEAPKAQPIPAGEYPETREKMSGIRKAIAKAMVNSKHTAPHVTLMDEVDVTELVAHR
KKFKAVAADKGIKLTYLPYVVKALTSALREYPMLNTSLDDASQEVVHKHYFNIGIAADTDKGLLVPVVKDTDRKSIFTIS
NEINDLAGKAREGRLAPAEMKGASCTITNIGSAGGQWFTPVINHPEVAILGIGRIAEKPVVKNGEIVAAPVLALSLSFDH
RLIDGATAQKALNQIKRLLNDPQLLVMEA
>Mature_428_residues
AFEFKLPDIGEGIHEGEIVKWFIKPGDEVNEDDVLLEVQNDKAVVEIPSPVKGKVLEVLVEEGTVAVVGDTLIKFDAPGY
ENLKFKGDDHDEAPKAEEAKEEAPKAEATPAATAEVVNERVIAMPSVRKYARENGVDIHKVAGSGKNGRIVKADIDAFAN
GGQAVAATEAPAAVEATPAAAKEEAPKAQPIPAGEYPETREKMSGIRKAIAKAMVNSKHTAPHVTLMDEVDVTELVAHRK
KFKAVAADKGIKLTYLPYVVKALTSALREYPMLNTSLDDASQEVVHKHYFNIGIAADTDKGLLVPVVKDTDRKSIFTISN
EINDLAGKAREGRLAPAEMKGASCTITNIGSAGGQWFTPVINHPEVAILGIGRIAEKPVVKNGEIVAAPVLALSLSFDHR
LIDGATAQKALNQIKRLLNDPQLLVMEA

Specific function: The B.subtilis PDH complex possesses also branched-chain 2-oxoacid dehydrogenase (BCDH) activity [H]

COG id: COG0508

COG function: function code C; Pyruvate/2-oxoglutarate dehydrogenase complex, dihydrolipoamide acyltransferase (E2) component, and related enzymes

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 lipoyl-binding domain [H]

Homologues:

Organism=Homo sapiens, GI110671329, Length=432, Percent_Identity=35.1851851851852, Blast_Score=248, Evalue=8e-66,
Organism=Homo sapiens, GI31711992, Length=441, Percent_Identity=29.4784580498866, Blast_Score=176, Evalue=3e-44,
Organism=Homo sapiens, GI203098753, Length=447, Percent_Identity=28.6353467561521, Blast_Score=154, Evalue=2e-37,
Organism=Homo sapiens, GI203098816, Length=447, Percent_Identity=28.6353467561521, Blast_Score=154, Evalue=2e-37,
Organism=Homo sapiens, GI260898739, Length=160, Percent_Identity=34.375, Blast_Score=93, Evalue=4e-19,
Organism=Escherichia coli, GI1786305, Length=433, Percent_Identity=35.796766743649, Blast_Score=231, Evalue=1e-61,
Organism=Escherichia coli, GI1786946, Length=432, Percent_Identity=31.25, Blast_Score=209, Evalue=4e-55,
Organism=Caenorhabditis elegans, GI17537937, Length=429, Percent_Identity=33.5664335664336, Blast_Score=234, Evalue=6e-62,
Organism=Caenorhabditis elegans, GI25146366, Length=423, Percent_Identity=32.8605200945626, Blast_Score=198, Evalue=4e-51,
Organism=Caenorhabditis elegans, GI17560088, Length=450, Percent_Identity=29.7777777777778, Blast_Score=175, Evalue=3e-44,
Organism=Caenorhabditis elegans, GI17538894, Length=308, Percent_Identity=28.8961038961039, Blast_Score=121, Evalue=6e-28,
Organism=Saccharomyces cerevisiae, GI6320352, Length=435, Percent_Identity=31.7241379310345, Blast_Score=188, Evalue=2e-48,
Organism=Saccharomyces cerevisiae, GI6324258, Length=446, Percent_Identity=28.2511210762332, Blast_Score=151, Evalue=2e-37,
Organism=Drosophila melanogaster, GI18859875, Length=431, Percent_Identity=36.6589327146172, Blast_Score=255, Evalue=3e-68,
Organism=Drosophila melanogaster, GI24645909, Length=233, Percent_Identity=33.0472103004292, Blast_Score=135, Evalue=7e-32,
Organism=Drosophila melanogaster, GI24582497, Length=228, Percent_Identity=30.7017543859649, Blast_Score=123, Evalue=3e-28,
Organism=Drosophila melanogaster, GI20129315, Length=228, Percent_Identity=30.7017543859649, Blast_Score=122, Evalue=5e-28,

Paralogues:

None

Copy number: 1120 Molecules/Cell In: Stationary Phase, Rich Media (Based on E. coli). 912 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. 4,000 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003016
- InterPro:   IPR001078
- InterPro:   IPR000089
- InterPro:   IPR023213
- InterPro:   IPR004167
- InterPro:   IPR011053 [H]

Pfam domain/function: PF00198 2-oxoacid_dh; PF00364 Biotin_lipoyl; PF02817 E3_binding [H]

EC number: =2.3.1.12 [H]

Molecular weight: Translated: 45985; Mature: 45854

Theoretical pI: Translated: 5.27; Mature: 5.27

Prosite motif: PS50968 BIOTINYL_LIPOYL ; PS00189 LIPOYL

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.2 %Cys     (Translated Protein)
1.9 %Met     (Translated Protein)
2.1 %Cys+Met (Translated Protein)
0.2 %Cys     (Mature Protein)
1.6 %Met     (Mature Protein)
1.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAFEFKLPDIGEGIHEGEIVKWFIKPGDEVNEDDVLLEVQNDKAVVEIPSPVKGKVLEVL
CCEEECCCCCCCCCCCCCEEEEEECCCCCCCCCCEEEEECCCEEEEECCCCCCHHHHHHH
VEEGTVAVVGDTLIKFDAPGYENLKFKGDDHDEAPKAEEAKEEAPKAEATPAATAEVVNE
HHCCCEEEEECEEEEECCCCCCCCEECCCCCCCCCCHHHHHHHCCCCCCCCHHHHHHHHC
RVIAMPSVRKYARENGVDIHKVAGSGKNGRIVKADIDAFANGGQAVAATEAPAAVEATPA
CEEECCHHHHHHHHCCCEEEEECCCCCCCEEEEEEHHHHCCCCCEEEECCCCCCEECCCC
AAKEEAPKAQPIPAGEYPETREKMSGIRKAIAKAMVNSKHTAPHVTLMDEVDVTELVAHR
HHHHCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHCCCCCCCEEEEECCCCHHHHHHHH
KKFKAVAADKGIKLTYLPYVVKALTSALREYPMLNTSLDDASQEVVHKHYFNIGIAADTD
HHHHHHHCCCCCEEEHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHEEEEEECCC
KGLLVPVVKDTDRKSIFTISNEINDLAGKAREGRLAPAEMKGASCTITNIGSAGGQWFTP
CCEEEEEECCCCCCEEEEEHHHHHHHHCCCCCCCCCHHHCCCCEEEEEECCCCCCCEECC
VINHPEVAILGIGRIAEKPVVKNGEIVAAPVLALSLSFDHRLIDGATAQKALNQIKRLLN
CCCCCCEEEEECCHHCCCCCCCCCCEEEEEEEEEEECCCCHHHCCHHHHHHHHHHHHHHC
DPQLLVMEA
CCCEEEEEC
>Mature Secondary Structure 
AFEFKLPDIGEGIHEGEIVKWFIKPGDEVNEDDVLLEVQNDKAVVEIPSPVKGKVLEVL
CEEECCCCCCCCCCCCCEEEEEECCCCCCCCCCEEEEECCCEEEEECCCCCCHHHHHHH
VEEGTVAVVGDTLIKFDAPGYENLKFKGDDHDEAPKAEEAKEEAPKAEATPAATAEVVNE
HHCCCEEEEECEEEEECCCCCCCCEECCCCCCCCCCHHHHHHHCCCCCCCCHHHHHHHHC
RVIAMPSVRKYARENGVDIHKVAGSGKNGRIVKADIDAFANGGQAVAATEAPAAVEATPA
CEEECCHHHHHHHHCCCEEEEECCCCCCCEEEEEEHHHHCCCCCEEEECCCCCCEECCCC
AAKEEAPKAQPIPAGEYPETREKMSGIRKAIAKAMVNSKHTAPHVTLMDEVDVTELVAHR
HHHHCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHCCCCCCCEEEEECCCCHHHHHHHH
KKFKAVAADKGIKLTYLPYVVKALTSALREYPMLNTSLDDASQEVVHKHYFNIGIAADTD
HHHHHHHCCCCCEEEHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHEEEEEECCC
KGLLVPVVKDTDRKSIFTISNEINDLAGKAREGRLAPAEMKGASCTITNIGSAGGQWFTP
CCEEEEEECCCCCCEEEEEHHHHHHHHCCCCCCCCCHHHCCCCEEEEEECCCCCCCEECC
VINHPEVAILGIGRIAEKPVVKNGEIVAAPVLALSLSFDHRLIDGATAQKALNQIKRLLN
CCCCCCEEEEECCHHCCCCCCCCCCEEEEEEEEEEECCCCHHHCCHHHHHHHHHHHHHHC
DPQLLVMEA
CCCEEEEEC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 1697575; 8969500; 9384377 [H]