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The map label for this gene is cwlH [H]

Identifier: 218904808

GI number: 218904808

Start: 3524581

End: 3526308

Strand: Reverse

Name: cwlH [H]

Synonym: BCAH820_3692

Alternate gene names: 218904808

Gene position: 3526308-3524581 (Counterclockwise)

Preceding gene: 218904809

Following gene: 218904807

Centisome position: 66.5

GC content: 36.28

Gene sequence:

>1728_bases
ATGAAAAAAGGATTTTATAATGTTTTAGCTGCATCAATTGTATTTAGTATGGCAACGATTCCAAATTATTCCTATGCTAA
CGAACTTGACAAAACGGTTACAGTTTCACCTGATGAACAAGCTTTAAAAAGTATTGAAAACCATATGAAAGATGAAGATG
GTAGAGGAGAAGATAAAGGAGTAAGAAATGAAGTGCAAGGTGAATTTCTTGTACATATAGTGAAAGAGGTACCTTTATAC
GATTCTAGTAATTTTCAAAAAGAGACAGGGGTTCGTATTTCGAATCAAGTCGTAAAAGCTGAGAAGAGAAAAGGAAACGC
TTACTATGTTCAAACTTCATCGGGAACTGGTTGGATACAAAATAGCGATGGGAATGTAGAAGTTAAAGAAATTCACCCGT
TATTAAGTGAGAAGTTAATTGTAAATGAAGAAACCTCTACATACTCTGAACCATTTGCTTCATACAAAGAAGAAAATGTA
CTGGAGCCACAAACTATTCAAGCAATTGGACAGGCTGGAGAATGGTTCCAAGTAAAAATAAATAATGAGATGAAATGGAT
TCATTCACCTTCAGCAAAATTTGAAGGAACAAAAGCTTCGCTTATACAAGGCGCAGCACCAATACGTACAAAATATGCAG
CAGCTATGTATGCTGCACCAATAGAAGAGAAGACAACGGATATTTATGGTGTACCTTTAAAAGAAATGATTGTACCGAAA
GGAAATGAAAATATTCGTCCGGGTTATGCGATGAATCCGAAGTATATTACGATTCATGAGACAGATAATTACAGTGTTGG
GGCAAATGCTAGAAACCATGCGATATATTTATATAATCAAGCAACGGGAACGGAAGATCGTTCAGCGTCGTGGCATTTTA
CAGTAGATGATAAAGAGATTTATCAACACCTGCCATTAAACGAAAATGCTTGGCATGCAGGAGATGGGGCAGAAGGAACT
GGAAATCGAGAATCAATTGCAATTGAAATTGCTGTAAACGAAGATGGTGATTATAATAAAGCCGTTGAAAATGCTCGTAA
ACTAGCAGCTTATTTAATGGGGGAATTAAATATTCCTTTAGAGAATGTAAAAAAACATCAATTTTGGAGTGGGAAGATTT
GTCCAGCCATTATGATTAAAAATAATGGCTGGGAACCATTCTTACAAGGAACAAAAGTGTATTATGATGCGAACCATAAA
GATGATATAACGGGTGGTTGGTACGAAGCAGACATTCGTGAATTAGATAAGCGCGGCATTATGGTTGGAGATGGGAAAGG
TTCTTATTGGCCAGAGCGTCTTGTGACACGTGGTGAATTTGCGAACTTCATTTCAAGATCATTGCAGTTACCTGAAGGGT
CATCGAATTTTAGTGACTTAAATGCAGCACATCCATCGCTAATAGATGGTATTAATCGTGCAGCAAGTGCCGGGATAATA
AGTGGTAGAGGAAATGGGATATTTGCTCCAAATGATACGATTAAACGAGATGAAGTTGTTATCATGATTGACCGTGCACT
GCAATATAAAAAAATTAAAGGTAACTTAGTTCCACTACCATTCTCGGATCAAGATTTAGCATATGATAAACAAGCTGTAC
AACGTGTTTATGGATTAGGCATTGTAAAAGGAAATGAAAATAATGAATTTATGCCGAAAGGTTCAGCAACGCGTGGAGAA
TCAGCAGCATTTATTAATAGAATGTTAGAAGTAATAGAAAGTAATTAA

Upstream 100 bases:

>100_bases
TAAATGGTTATGTTGTTTAATAGTTTAGTAGATAACCTATATTTTGGCTGAGGAGGGGAAATTTAACACGAACCTAATTA
TATGCGTGTTAAGTAGTATG

Downstream 100 bases:

>100_bases
TAAAAAAGCATTCGCTTTTGCGAGTGCTTTTTTATTTTTCAATATGTCACGGAAGGGAGTGGAGGTTTTTGAGTGGAATA
AGGAATGAAAGTGGAGGTGA

Product: surface-layer N-acetylmuramoyl-L-alanine amidase

Products: NA

Alternate protein names: Autolysin; Cell wall hydrolase [H]

Number of amino acids: Translated: 575; Mature: 575

Protein sequence:

>575_residues
MKKGFYNVLAASIVFSMATIPNYSYANELDKTVTVSPDEQALKSIENHMKDEDGRGEDKGVRNEVQGEFLVHIVKEVPLY
DSSNFQKETGVRISNQVVKAEKRKGNAYYVQTSSGTGWIQNSDGNVEVKEIHPLLSEKLIVNEETSTYSEPFASYKEENV
LEPQTIQAIGQAGEWFQVKINNEMKWIHSPSAKFEGTKASLIQGAAPIRTKYAAAMYAAPIEEKTTDIYGVPLKEMIVPK
GNENIRPGYAMNPKYITIHETDNYSVGANARNHAIYLYNQATGTEDRSASWHFTVDDKEIYQHLPLNENAWHAGDGAEGT
GNRESIAIEIAVNEDGDYNKAVENARKLAAYLMGELNIPLENVKKHQFWSGKICPAIMIKNNGWEPFLQGTKVYYDANHK
DDITGGWYEADIRELDKRGIMVGDGKGSYWPERLVTRGEFANFISRSLQLPEGSSNFSDLNAAHPSLIDGINRAASAGII
SGRGNGIFAPNDTIKRDEVVIMIDRALQYKKIKGNLVPLPFSDQDLAYDKQAVQRVYGLGIVKGNENNEFMPKGSATRGE
SAAFINRMLEVIESN

Sequences:

>Translated_575_residues
MKKGFYNVLAASIVFSMATIPNYSYANELDKTVTVSPDEQALKSIENHMKDEDGRGEDKGVRNEVQGEFLVHIVKEVPLY
DSSNFQKETGVRISNQVVKAEKRKGNAYYVQTSSGTGWIQNSDGNVEVKEIHPLLSEKLIVNEETSTYSEPFASYKEENV
LEPQTIQAIGQAGEWFQVKINNEMKWIHSPSAKFEGTKASLIQGAAPIRTKYAAAMYAAPIEEKTTDIYGVPLKEMIVPK
GNENIRPGYAMNPKYITIHETDNYSVGANARNHAIYLYNQATGTEDRSASWHFTVDDKEIYQHLPLNENAWHAGDGAEGT
GNRESIAIEIAVNEDGDYNKAVENARKLAAYLMGELNIPLENVKKHQFWSGKICPAIMIKNNGWEPFLQGTKVYYDANHK
DDITGGWYEADIRELDKRGIMVGDGKGSYWPERLVTRGEFANFISRSLQLPEGSSNFSDLNAAHPSLIDGINRAASAGII
SGRGNGIFAPNDTIKRDEVVIMIDRALQYKKIKGNLVPLPFSDQDLAYDKQAVQRVYGLGIVKGNENNEFMPKGSATRGE
SAAFINRMLEVIESN
>Mature_575_residues
MKKGFYNVLAASIVFSMATIPNYSYANELDKTVTVSPDEQALKSIENHMKDEDGRGEDKGVRNEVQGEFLVHIVKEVPLY
DSSNFQKETGVRISNQVVKAEKRKGNAYYVQTSSGTGWIQNSDGNVEVKEIHPLLSEKLIVNEETSTYSEPFASYKEENV
LEPQTIQAIGQAGEWFQVKINNEMKWIHSPSAKFEGTKASLIQGAAPIRTKYAAAMYAAPIEEKTTDIYGVPLKEMIVPK
GNENIRPGYAMNPKYITIHETDNYSVGANARNHAIYLYNQATGTEDRSASWHFTVDDKEIYQHLPLNENAWHAGDGAEGT
GNRESIAIEIAVNEDGDYNKAVENARKLAAYLMGELNIPLENVKKHQFWSGKICPAIMIKNNGWEPFLQGTKVYYDANHK
DDITGGWYEADIRELDKRGIMVGDGKGSYWPERLVTRGEFANFISRSLQLPEGSSNFSDLNAAHPSLIDGINRAASAGII
SGRGNGIFAPNDTIKRDEVVIMIDRALQYKKIKGNLVPLPFSDQDLAYDKQAVQRVYGLGIVKGNENNEFMPKGSATRGE
SAAFINRMLEVIESN

Specific function: Autolysins are involved in some important biological processes such as cell separation, cell-wall turnover, competence for genetic transformation, formation of the flagella and sporulation. Could play a role in mother cell lysis with CwlC [H]

COG id: COG5632

COG function: function code M; N-acetylmuramoyl-L-alanine amidase

Gene ontology:

Cell location: Secreted (Potential) [H]

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Belongs to the N-acetylmuramoyl-L-alanine amidase 2 family [H]

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002502
- InterPro:   IPR002477 [H]

Pfam domain/function: PF01510 Amidase_2; PF01471 PG_binding_1 [H]

EC number: =3.5.1.28 [H]

Molecular weight: Translated: 64029; Mature: 64029

Theoretical pI: Translated: 5.33; Mature: 5.33

Prosite motif: PS00213 LIPOCALIN

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.2 %Cys     (Translated Protein)
2.3 %Met     (Translated Protein)
2.4 %Cys+Met (Translated Protein)
0.2 %Cys     (Mature Protein)
2.3 %Met     (Mature Protein)
2.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKKGFYNVLAASIVFSMATIPNYSYANELDKTVTVSPDEQALKSIENHMKDEDGRGEDKG
CCCHHHHHHHHHHHHHHHHCCCCHHHHHCCCEEEECCHHHHHHHHHHHHCCCCCCCCCCC
VRNEVQGEFLVHIVKEVPLYDSSNFQKETGVRISNQVVKAEKRKGNAYYVQTSSGTGWIQ
CHHHHHHHHHHHHHHHCCCCCCCCCCHHCCCCHHHHHHHHHHCCCCEEEEEECCCCCEEE
NSDGNVEVKEIHPLLSEKLIVNEETSTYSEPFASYKEENVLEPQTIQAIGQAGEWFQVKI
CCCCCEEHHHHHHHHHCCEEEECCCCCHHHHHHHHHHCCCCCHHHHHHHCCCCCEEEEEE
NNEMKWIHSPSAKFEGTKASLIQGAAPIRTKYAAAMYAAPIEEKTTDIYGVPLKEMIVPK
CCCEEEEECCCCCCCCCHHHHHCCCCCHHHHHHHHHHHCCCCCCCCCEECCCHHHHCCCC
GNENIRPGYAMNPKYITIHETDNYSVGANARNHAIYLYNQATGTEDRSASWHFTVDDKEI
CCCCCCCCCCCCCEEEEEECCCCEEECCCCCCCEEEEEECCCCCCCCCCEEEEEECHHHH
YQHLPLNENAWHAGDGAEGTGNRESIAIEIAVNEDGDYNKAVENARKLAAYLMGELNIPL
HHHCCCCCCCCCCCCCCCCCCCCCEEEEEEEECCCCCHHHHHHHHHHHHHHHHHCCCCCH
ENVKKHQFWSGKICPAIMIKNNGWEPFLQGTKVYYDANHKDDITGGWYEADIRELDKRGI
HHHHHCCCCCCCCCEEEEEECCCCCHHHCCCEEEECCCCCCCCCCCEEHHHHHHHHHCCE
MVGDGKGSYWPERLVTRGEFANFISRSLQLPEGSSNFSDLNAAHPSLIDGINRAASAGII
EEECCCCCCCHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCHHHHHHHHHHHCCEE
SGRGNGIFAPNDTIKRDEVVIMIDRALQYKKIKGNLVPLPFSDQDLAYDKQAVQRVYGLG
ECCCCEEECCCCCCCCCCEEEEECCCHHHHHHCCCEEECCCCCCCCHHHHHHHHHHHHCE
IVKGNENNEFMPKGSATRGESAAFINRMLEVIESN
EEECCCCCCCCCCCCCCCCCHHHHHHHHHHHHHCC
>Mature Secondary Structure
MKKGFYNVLAASIVFSMATIPNYSYANELDKTVTVSPDEQALKSIENHMKDEDGRGEDKG
CCCHHHHHHHHHHHHHHHHCCCCHHHHHCCCEEEECCHHHHHHHHHHHHCCCCCCCCCCC
VRNEVQGEFLVHIVKEVPLYDSSNFQKETGVRISNQVVKAEKRKGNAYYVQTSSGTGWIQ
CHHHHHHHHHHHHHHHCCCCCCCCCCHHCCCCHHHHHHHHHHCCCCEEEEEECCCCCEEE
NSDGNVEVKEIHPLLSEKLIVNEETSTYSEPFASYKEENVLEPQTIQAIGQAGEWFQVKI
CCCCCEEHHHHHHHHHCCEEEECCCCCHHHHHHHHHHCCCCCHHHHHHHCCCCCEEEEEE
NNEMKWIHSPSAKFEGTKASLIQGAAPIRTKYAAAMYAAPIEEKTTDIYGVPLKEMIVPK
CCCEEEEECCCCCCCCCHHHHHCCCCCHHHHHHHHHHHCCCCCCCCCEECCCHHHHCCCC
GNENIRPGYAMNPKYITIHETDNYSVGANARNHAIYLYNQATGTEDRSASWHFTVDDKEI
CCCCCCCCCCCCCEEEEEECCCCEEECCCCCCCEEEEEECCCCCCCCCCEEEEEECHHHH
YQHLPLNENAWHAGDGAEGTGNRESIAIEIAVNEDGDYNKAVENARKLAAYLMGELNIPL
HHHCCCCCCCCCCCCCCCCCCCCCEEEEEEEECCCCCHHHHHHHHHHHHHHHHHCCCCCH
ENVKKHQFWSGKICPAIMIKNNGWEPFLQGTKVYYDANHKDDITGGWYEADIRELDKRGI
HHHHHCCCCCCCCCEEEEEECCCCCHHHCCCEEEECCCCCCCCCCCEEHHHHHHHHHCCE
MVGDGKGSYWPERLVTRGEFANFISRSLQLPEGSSNFSDLNAAHPSLIDGINRAASAGII
EEECCCCCCCHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCHHHHHHHHHHHCCEE
SGRGNGIFAPNDTIKRDEVVIMIDRALQYKKIKGNLVPLPFSDQDLAYDKQAVQRVYGLG
ECCCCEEECCCCCCCCCCEEEEECCCHHHHHHCCCEEECCCCCCCCHHHHHHHHHHHHCE
IVKGNENNEFMPKGSATRGESAAFINRMLEVIESN
EEECCCCCCCCCCCCCCCCCHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8969508; 9384377 [H]