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The map label for this gene is yhfT [H]

Identifier: 218904755

GI number: 218904755

Start: 3471890

End: 3473353

Strand: Reverse

Name: yhfT [H]

Synonym: BCAH820_3639

Alternate gene names: 218904755

Gene position: 3473353-3471890 (Counterclockwise)

Preceding gene: 218904756

Following gene: 218904754

Centisome position: 65.5

GC content: 34.49

Gene sequence:

>1464_bases
ATGGGAATAACAAAAGAATATAAAAAACATGCCTCTTTACAACCGAATAAAATAGCAATAAAGGAAAATGATCGAGTCTT
AACATATAAAGAGTGGTTTGAGTCAGTGTATAAAGTAGCAAACTGGTTGAATGAAAAAGAATCGAAGAATAAAACGATAG
CAATTGTATTAGAAAATCGTATAGAGTTTTTACAACTATTCGCTAGTGCTGCTATGGCAGGATGGGTTTGTGTGCCACTA
GATATAAAGTGGAAACAAGATGAGCTCAAAGAAAGAATTGCAATTAGTAATCCGGATATGATTGTGACAGAGAGATATAA
GTTAAATGATTTACCGGATGAAGAAGGAAGAGTAATTGAAATTGATGAGTGGAAAAGGATGATTGAGAAATATCTTCCTA
CATATTTCCCTATAGAGAATGTACAAAATGCTCCTTTTTATATGGGATTCACATCAGGATCGACTGGAAAAGCAAAAGCG
TTTTTACGTGCGCAACAATCGTGGCTTCATAGTTTTGATTGTAATGTACATGATTTTCATATGAAAAGAGAGGATTCGAT
TTTAATAGCTGGGACGCTTGTTCATTCTCTTTTCTTATACGGGGCAATAAGCGCATTATATGTAGGACAAACGGTTCACA
TTATGAGAAAGTTCATTCCAAATCAAGTATTAGATCAGTTAGAAACTGAAAATATACCAGTTATGTATACAGTTCCGACA
ATGCTTGAATCTCTATATAAAGAAAATAGAGTGATAGAAAATAAAATGAAAATTATTTCGTCAGGGGCGAAATGGGAAGC
TGGAGCGAAAGAAAAAATAAAGAATATCTTTCCTTATGCGAAAAGATATGAATTTTATGGTGCATCGGAACTGAGTTTTG
TAACAGCATTAGTTGATGAAGAGAGCGAAAGAAGGCCAAATTCAGTAGGGAAACCTTGTCACAATGTGCAAGTTCGAGTA
TGTAATGAGGCAGGAAAAGAAGTACAGAAAGGTGAGATAGGAACTGTTTATGTGAAAAGTGATCAGTTTTTTATGGGATA
CATAATAGATGGTGTTTTAGCACGGGAGTTGAATGCAGATGGCTGGATGACAGTGCGAGATGTAGGTTATGAAGATGAAG
AAGGATTTATTTATATTATCGGTAGAGAGAAGAATATGATTTTATTTGGAGGAATTAATATTTTCCCAGAAGAAATAGAA
AGCGTATTACATGAACATCCAGCTGTTGATGAAATAGTTGTAATTGGTGTGGAAGATAGTTACTGGGGTGAAAAACCTGT
CGCGATTGTAAAAGGAAGTGCTACGAGGCAACAATTAAAGCGTTTTTGCTTACAAAGATTATCCTCTTTTAAAATACCGA
AAGAATGGTATTTTGTAGATGAAATACCGTATACAAATAGCGGGAAAATCGCTCGTATGGAAGCAAAGAGTATCATTGAA
AATCAGGAGAAAATATATGAATAG

Upstream 100 bases:

>100_bases
CTCTAAAGAATGGGAAATATACAAATGCGGGATAAGAAAGAGAAGAAACACGTTTATTGCGTGTTTCTTTTTAGTATTTT
GTAAAGAAAGGACATCTCAT

Downstream 100 bases:

>100_bases
AGCAGTTATTGTAGAAGCGAAAAGGACACCTATTGGTAAGAAGAATGGGATGTTAAAAGACTATGAAGTTCAGCAATTAG
CAGCACCGCTTCTTACATTT

Product: acyl-CoA synthetase

Products: AMP; diphosphate; acyl-CoA

Alternate protein names: NA

Number of amino acids: Translated: 487; Mature: 486

Protein sequence:

>487_residues
MGITKEYKKHASLQPNKIAIKENDRVLTYKEWFESVYKVANWLNEKESKNKTIAIVLENRIEFLQLFASAAMAGWVCVPL
DIKWKQDELKERIAISNPDMIVTERYKLNDLPDEEGRVIEIDEWKRMIEKYLPTYFPIENVQNAPFYMGFTSGSTGKAKA
FLRAQQSWLHSFDCNVHDFHMKREDSILIAGTLVHSLFLYGAISALYVGQTVHIMRKFIPNQVLDQLETENIPVMYTVPT
MLESLYKENRVIENKMKIISSGAKWEAGAKEKIKNIFPYAKRYEFYGASELSFVTALVDEESERRPNSVGKPCHNVQVRV
CNEAGKEVQKGEIGTVYVKSDQFFMGYIIDGVLARELNADGWMTVRDVGYEDEEGFIYIIGREKNMILFGGINIFPEEIE
SVLHEHPAVDEIVVIGVEDSYWGEKPVAIVKGSATRQQLKRFCLQRLSSFKIPKEWYFVDEIPYTNSGKIARMEAKSIIE
NQEKIYE

Sequences:

>Translated_487_residues
MGITKEYKKHASLQPNKIAIKENDRVLTYKEWFESVYKVANWLNEKESKNKTIAIVLENRIEFLQLFASAAMAGWVCVPL
DIKWKQDELKERIAISNPDMIVTERYKLNDLPDEEGRVIEIDEWKRMIEKYLPTYFPIENVQNAPFYMGFTSGSTGKAKA
FLRAQQSWLHSFDCNVHDFHMKREDSILIAGTLVHSLFLYGAISALYVGQTVHIMRKFIPNQVLDQLETENIPVMYTVPT
MLESLYKENRVIENKMKIISSGAKWEAGAKEKIKNIFPYAKRYEFYGASELSFVTALVDEESERRPNSVGKPCHNVQVRV
CNEAGKEVQKGEIGTVYVKSDQFFMGYIIDGVLARELNADGWMTVRDVGYEDEEGFIYIIGREKNMILFGGINIFPEEIE
SVLHEHPAVDEIVVIGVEDSYWGEKPVAIVKGSATRQQLKRFCLQRLSSFKIPKEWYFVDEIPYTNSGKIARMEAKSIIE
NQEKIYE
>Mature_486_residues
GITKEYKKHASLQPNKIAIKENDRVLTYKEWFESVYKVANWLNEKESKNKTIAIVLENRIEFLQLFASAAMAGWVCVPLD
IKWKQDELKERIAISNPDMIVTERYKLNDLPDEEGRVIEIDEWKRMIEKYLPTYFPIENVQNAPFYMGFTSGSTGKAKAF
LRAQQSWLHSFDCNVHDFHMKREDSILIAGTLVHSLFLYGAISALYVGQTVHIMRKFIPNQVLDQLETENIPVMYTVPTM
LESLYKENRVIENKMKIISSGAKWEAGAKEKIKNIFPYAKRYEFYGASELSFVTALVDEESERRPNSVGKPCHNVQVRVC
NEAGKEVQKGEIGTVYVKSDQFFMGYIIDGVLARELNADGWMTVRDVGYEDEEGFIYIIGREKNMILFGGINIFPEEIES
VLHEHPAVDEIVVIGVEDSYWGEKPVAIVKGSATRQQLKRFCLQRLSSFKIPKEWYFVDEIPYTNSGKIARMEAKSIIEN
QEKIYE

Specific function: May be involved in fatty acid metabolism [H]

COG id: COG0318

COG function: function code IQ; Acyl-CoA synthetases (AMP-forming)/AMP-acid ligases II

Gene ontology:

Cell location: Partially Membrane-Associated [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the ATP-dependent AMP-binding enzyme family [H]

Homologues:

Organism=Homo sapiens, GI156151445, Length=374, Percent_Identity=27.5401069518717, Blast_Score=121, Evalue=2e-27,
Organism=Homo sapiens, GI187761345, Length=516, Percent_Identity=25, Blast_Score=112, Evalue=1e-24,
Organism=Homo sapiens, GI187761343, Length=516, Percent_Identity=25, Blast_Score=112, Evalue=1e-24,
Organism=Homo sapiens, GI157311624, Length=502, Percent_Identity=24.3027888446215, Blast_Score=89, Evalue=9e-18,
Organism=Homo sapiens, GI157311622, Length=502, Percent_Identity=24.3027888446215, Blast_Score=89, Evalue=9e-18,
Organism=Homo sapiens, GI58082049, Length=501, Percent_Identity=23.1536926147705, Blast_Score=88, Evalue=2e-17,
Organism=Homo sapiens, GI42544132, Length=284, Percent_Identity=27.8169014084507, Blast_Score=87, Evalue=3e-17,
Organism=Homo sapiens, GI38505220, Length=357, Percent_Identity=25.2100840336134, Blast_Score=82, Evalue=1e-15,
Organism=Homo sapiens, GI122937307, Length=371, Percent_Identity=25.8760107816712, Blast_Score=80, Evalue=5e-15,
Organism=Homo sapiens, GI115511026, Length=495, Percent_Identity=23.2323232323232, Blast_Score=79, Evalue=9e-15,
Organism=Homo sapiens, GI13375727, Length=427, Percent_Identity=21.0772833723653, Blast_Score=75, Evalue=1e-13,
Organism=Escherichia coli, GI1788107, Length=514, Percent_Identity=24.5136186770428, Blast_Score=130, Evalue=3e-31,
Organism=Escherichia coli, GI145693145, Length=503, Percent_Identity=24.2544731610338, Blast_Score=123, Evalue=3e-29,
Organism=Escherichia coli, GI1786810, Length=510, Percent_Identity=24.3137254901961, Blast_Score=112, Evalue=5e-26,
Organism=Escherichia coli, GI221142682, Length=202, Percent_Identity=32.6732673267327, Blast_Score=101, Evalue=1e-22,
Organism=Escherichia coli, GI1788595, Length=337, Percent_Identity=25.5192878338279, Blast_Score=92, Evalue=1e-19,
Organism=Escherichia coli, GI1790505, Length=399, Percent_Identity=22.5563909774436, Blast_Score=78, Evalue=1e-15,
Organism=Caenorhabditis elegans, GI17531443, Length=527, Percent_Identity=24.4781783681214, Blast_Score=129, Evalue=4e-30,
Organism=Caenorhabditis elegans, GI32563687, Length=486, Percent_Identity=25.9259259259259, Blast_Score=126, Evalue=2e-29,
Organism=Caenorhabditis elegans, GI17559526, Length=507, Percent_Identity=24.0631163708087, Blast_Score=120, Evalue=2e-27,
Organism=Caenorhabditis elegans, GI17538037, Length=301, Percent_Identity=30.8970099667774, Blast_Score=115, Evalue=5e-26,
Organism=Caenorhabditis elegans, GI17557194, Length=359, Percent_Identity=27.2980501392758, Blast_Score=113, Evalue=2e-25,
Organism=Caenorhabditis elegans, GI17560308, Length=363, Percent_Identity=27.8236914600551, Blast_Score=106, Evalue=3e-23,
Organism=Caenorhabditis elegans, GI71985884, Length=361, Percent_Identity=25.7617728531856, Blast_Score=106, Evalue=3e-23,
Organism=Caenorhabditis elegans, GI71994694, Length=349, Percent_Identity=26.647564469914, Blast_Score=105, Evalue=4e-23,
Organism=Caenorhabditis elegans, GI71994703, Length=349, Percent_Identity=26.647564469914, Blast_Score=105, Evalue=4e-23,
Organism=Caenorhabditis elegans, GI71994690, Length=349, Percent_Identity=26.647564469914, Blast_Score=105, Evalue=5e-23,
Organism=Caenorhabditis elegans, GI17558820, Length=495, Percent_Identity=24.2424242424242, Blast_Score=103, Evalue=2e-22,
Organism=Caenorhabditis elegans, GI71996755, Length=490, Percent_Identity=23.6734693877551, Blast_Score=97, Evalue=1e-20,
Organism=Caenorhabditis elegans, GI17560140, Length=495, Percent_Identity=23.8383838383838, Blast_Score=93, Evalue=3e-19,
Organism=Caenorhabditis elegans, GI25141359, Length=486, Percent_Identity=21.1934156378601, Blast_Score=83, Evalue=3e-16,
Organism=Saccharomyces cerevisiae, GI6319699, Length=524, Percent_Identity=24.8091603053435, Blast_Score=117, Evalue=3e-27,
Organism=Saccharomyces cerevisiae, GI6319264, Length=563, Percent_Identity=23.9786856127886, Blast_Score=96, Evalue=1e-20,
Organism=Drosophila melanogaster, GI24581924, Length=341, Percent_Identity=27.5659824046921, Blast_Score=119, Evalue=3e-27,
Organism=Drosophila melanogaster, GI21355181, Length=486, Percent_Identity=24.6913580246914, Blast_Score=117, Evalue=1e-26,
Organism=Drosophila melanogaster, GI24648260, Length=340, Percent_Identity=26.1764705882353, Blast_Score=105, Evalue=1e-22,
Organism=Drosophila melanogaster, GI21356947, Length=229, Percent_Identity=26.6375545851528, Blast_Score=101, Evalue=1e-21,
Organism=Drosophila melanogaster, GI18859661, Length=498, Percent_Identity=24.0963855421687, Blast_Score=100, Evalue=3e-21,
Organism=Drosophila melanogaster, GI24653035, Length=200, Percent_Identity=28, Blast_Score=94, Evalue=2e-19,
Organism=Drosophila melanogaster, GI21356441, Length=463, Percent_Identity=22.2462203023758, Blast_Score=92, Evalue=6e-19,
Organism=Drosophila melanogaster, GI24648253, Length=507, Percent_Identity=22.2879684418146, Blast_Score=91, Evalue=2e-18,
Organism=Drosophila melanogaster, GI24648255, Length=199, Percent_Identity=29.6482412060301, Blast_Score=89, Evalue=7e-18,
Organism=Drosophila melanogaster, GI21358303, Length=136, Percent_Identity=33.8235294117647, Blast_Score=89, Evalue=7e-18,
Organism=Drosophila melanogaster, GI19922652, Length=356, Percent_Identity=23.314606741573, Blast_Score=89, Evalue=9e-18,
Organism=Drosophila melanogaster, GI161076582, Length=207, Percent_Identity=28.5024154589372, Blast_Score=86, Evalue=8e-17,
Organism=Drosophila melanogaster, GI24656500, Length=344, Percent_Identity=25, Blast_Score=84, Evalue=2e-16,
Organism=Drosophila melanogaster, GI20130357, Length=206, Percent_Identity=25.7281553398058, Blast_Score=84, Evalue=2e-16,
Organism=Drosophila melanogaster, GI281365686, Length=226, Percent_Identity=26.1061946902655, Blast_Score=84, Evalue=2e-16,
Organism=Drosophila melanogaster, GI24667955, Length=402, Percent_Identity=25.1243781094527, Blast_Score=82, Evalue=8e-16,
Organism=Drosophila melanogaster, GI24667959, Length=402, Percent_Identity=25.1243781094527, Blast_Score=82, Evalue=1e-15,
Organism=Drosophila melanogaster, GI62472339, Length=402, Percent_Identity=24.8756218905473, Blast_Score=81, Evalue=1e-15,
Organism=Drosophila melanogaster, GI24648257, Length=199, Percent_Identity=25.1256281407035, Blast_Score=79, Evalue=9e-15,
Organism=Drosophila melanogaster, GI21355225, Length=468, Percent_Identity=22.8632478632479, Blast_Score=74, Evalue=3e-13,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR020845
- InterPro:   IPR000873 [H]

Pfam domain/function: PF00501 AMP-binding [H]

EC number: 6.2.1.3

Molecular weight: Translated: 56208; Mature: 56077

Theoretical pI: Translated: 5.76; Mature: 5.76

Prosite motif: PS00455 AMP_BINDING

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
2.9 %Met     (Translated Protein)
3.9 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
2.7 %Met     (Mature Protein)
3.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MGITKEYKKHASLQPNKIAIKENDRVLTYKEWFESVYKVANWLNEKESKNKTIAIVLENR
CCCCHHHHHHCCCCCCEEEEECCCCEEEHHHHHHHHHHHHHHHHHHCCCCCEEEEEEHHH
IEFLQLFASAAMAGWVCVPLDIKWKQDELKERIAISNPDMIVTERYKLNDLPDEEGRVIE
HHHHHHHHHHHHHCEEEEEEEEEECHHHHHHHHCCCCCCEEEEECEECCCCCCCCCCEEE
IDEWKRMIEKYLPTYFPIENVQNAPFYMGFTSGSTGKAKAFLRAQQSWLHSFDCNVHDFH
HHHHHHHHHHHCCCCCCCCCCCCCCEEEEECCCCCCHHHHHHHHHHHHHHHCCCCCEEEE
MKREDSILIAGTLVHSLFLYGAISALYVGQTVHIMRKFIPNQVLDQLETENIPVMYTVPT
ECCCCCEEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEEHHH
MLESLYKENRVIENKMKIISSGAKWEAGAKEKIKNIFPYAKRYEFYGASELSFVTALVDE
HHHHHHHHCHHHHHHHHHHHCCCCCCCCHHHHHHHHCCHHHHHCCCCCCHHHHHHHHHCC
ESERRPNSVGKPCHNVQVRVCNEAGKEVQKGEIGTVYVKSDQFFMGYIIDGVLARELNAD
HHHCCCCCCCCCCCCCEEEEECHHCCHHHCCCCEEEEEECCCEEEHHHHHHHHHHHCCCC
GWMTVRDVGYEDEEGFIYIIGREKNMILFGGINIFPEEIESVLHEHPAVDEIVVIGVEDS
CCEEEEECCCCCCCCEEEEEECCCCEEEEECCCCCHHHHHHHHHHCCCCCCEEEEEECCC
YWGEKPVAIVKGSATRQQLKRFCLQRLSSFKIPKEWYFVDEIPYTNSGKIARMEAKSIIE
CCCCCCEEEEECCHHHHHHHHHHHHHHHHCCCCCCEEEEECCCCCCCCCEEEHHHHHHHH
NQEKIYE
HHHHHCC
>Mature Secondary Structure 
GITKEYKKHASLQPNKIAIKENDRVLTYKEWFESVYKVANWLNEKESKNKTIAIVLENR
CCCHHHHHHCCCCCCEEEEECCCCEEEHHHHHHHHHHHHHHHHHHCCCCCEEEEEEHHH
IEFLQLFASAAMAGWVCVPLDIKWKQDELKERIAISNPDMIVTERYKLNDLPDEEGRVIE
HHHHHHHHHHHHHCEEEEEEEEEECHHHHHHHHCCCCCCEEEEECEECCCCCCCCCCEEE
IDEWKRMIEKYLPTYFPIENVQNAPFYMGFTSGSTGKAKAFLRAQQSWLHSFDCNVHDFH
HHHHHHHHHHHCCCCCCCCCCCCCCEEEEECCCCCCHHHHHHHHHHHHHHHCCCCCEEEE
MKREDSILIAGTLVHSLFLYGAISALYVGQTVHIMRKFIPNQVLDQLETENIPVMYTVPT
ECCCCCEEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEEHHH
MLESLYKENRVIENKMKIISSGAKWEAGAKEKIKNIFPYAKRYEFYGASELSFVTALVDE
HHHHHHHHCHHHHHHHHHHHCCCCCCCCHHHHHHHHCCHHHHHCCCCCCHHHHHHHHHCC
ESERRPNSVGKPCHNVQVRVCNEAGKEVQKGEIGTVYVKSDQFFMGYIIDGVLARELNAD
HHHCCCCCCCCCCCCCEEEEECHHCCHHHCCCCEEEEEECCCEEEHHHHHHHHHHHCCCC
GWMTVRDVGYEDEEGFIYIIGREKNMILFGGINIFPEEIESVLHEHPAVDEIVVIGVEDS
CCEEEEECCCCCCCCEEEEEECCCCEEEEECCCCCHHHHHHHHHHCCCCCCEEEEEECCC
YWGEKPVAIVKGSATRQQLKRFCLQRLSSFKIPKEWYFVDEIPYTNSGKIARMEAKSIIE
CCCCCCEEEEECCHHHHHHHHHHHHHHHHCCCCCCEEEEECCCCCCCCCEEEHHHHHHHH
NQEKIYE
HHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: Salts [C]

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): 0.0024 {myristate}} 0.0021 {myristate}} 0.0012 {myristate}} 0.0002 {myristate}} 0.0408 {octanoate}} 0.0125 {octanoate}} 0.0059 {octanoate}} 0.00072 {octanoate}} 0.0112 {hexanoate}} 0.0083 {decanoate}} 0.0063 {decanoate}} 0.0043 {dec

Substrates: ATP; long-chain carboxylic acid; CoA

Specific reaction: ATP + a long-chain carboxylic acid + CoA = AMP + diphosphate + an acyl-CoA

General reaction: Acid-thiol ligation; Phosphorylation [C]

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 9384377 [H]