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The map label for this gene is alaXL [H]

Identifier: 218904751

GI number: 218904751

Start: 3468422

End: 3469624

Strand: Direct

Name: alaXL [H]

Synonym: BCAH820_3635

Alternate gene names: 218904751

Gene position: 3468422-3469624 (Clockwise)

Preceding gene: 218904748

Following gene: 218904753

Centisome position: 65.41

GC content: 36.99

Gene sequence:

>1203_bases
TTGGAGCAAAAATTATATTACACTGACGCTTATAAGCAAGACTTTACTACAAAAGTTATAAAGCAAGATTATGATAAAGA
CGGTAACTTATACGTTATTTTAAACGAAACGGTATTTTACCCAACAGGTGGCGGGCAACCCCATGATACTGGAACTTTAA
ATGGCATTGCTGTACTTGGTGTTGAAGAAGTCGACGAAGAAATCCGCCACTTCATTGCAGAACAATTACATACAGAAGAA
GTAGAAGGTAAAATTAATTGGGAGCGCCGTTTTGATCATATGCAACAACATGCGGCTCAGCACATTTTATCTGCTGCTTT
TTGGGATTACTTTAACATACCTACGATTGGATTCCATCTTGGTAAAGAAACGGTAACAATTGATTTAGAAACAGAAAATT
TACATGCAGAAACGGTTGAAAAAGCAGTACAAATTGCGAACAAAATTGTTTTTGAAAATCATCCAATTCTTATTAAATGG
ATGAACTTAGAAGAAGCGAAAACATTACCACTTCGCAAAGAACCGACTATGACAGAAAATATACGCGTTGTTATGATCGA
AAACTTTGATTATAACGGCTGTGGCGGAACACACCCGAAACGTACAGGTGAAGTAGGGCCTATTCAAGTACTAGGATGGG
AACGTAATAAAGGCGGCATACGATTAACATTTATCGCTGGTTGGCGCACACTTAAATTAATGGGACAACAGCAACAAATT
ATGAAGGATGTTTCTAAACAATTAAACAGTAGCGAAGCTGATATTCCAGCAAAAGTAGCACAACTTCTTACTTCTCAAAA
AGAAAATGAAAAAGCAATGCAAACAATGACTGAAAAATTATTATATACAGAGGCAAACGAACTACTTCAACAACCTACCA
AAATACATGCTGGCATACTTATTTCTAAAGTGTTTACAAATCGTTCTATGCAAGAAATCGCAAAGCTATCCGCTATTATT
ACAGAACAACAAGAGCATGCCATTACATATTTCGTTATTGAAAACGAGGACAAATTACAATGCATCCTCGCTTGTGGAAA
AACAGTCACACTCGATATGAATGCTCTTTTAAAAGAGGCACTTCCTACAATTGAAGGAAAAGGTGGCGGAAATAAAAAGA
GTGCTCGCGGCGGTGGGAAAGCGATGATGAGCGGGGATGAGTTTTTAAATCAACTTATTTCTTCTTTACAGTCAGCAGTC
TAA

Upstream 100 bases:

>100_bases
GCAAGAAAATTCTAAAAATAGTTTCTTTCTTCTGAAATATTAGGAAACGAATTTCGATTGCTTTACAATGAAACTAGCGA
ACACGAAAGGGGAAAGAAAA

Downstream 100 bases:

>100_bases
AACCTGTTTATGAAGGATAGCTAACGGCTATCCTTCCACTATATTTATAGGCCTATATTTCGTTTTAACCCTTGATAACT
CCCTGCATTTAGAAAACCTT

Product: alanyl-tRNA synthetase family protein

Products: AMP; diphosphate; L-alanyl-tRNAAla [C]

Alternate protein names: AlaX-L; Alanyl-tRNA deacylase AlaX-L [H]

Number of amino acids: Translated: 400; Mature: 400

Protein sequence:

>400_residues
MEQKLYYTDAYKQDFTTKVIKQDYDKDGNLYVILNETVFYPTGGGQPHDTGTLNGIAVLGVEEVDEEIRHFIAEQLHTEE
VEGKINWERRFDHMQQHAAQHILSAAFWDYFNIPTIGFHLGKETVTIDLETENLHAETVEKAVQIANKIVFENHPILIKW
MNLEEAKTLPLRKEPTMTENIRVVMIENFDYNGCGGTHPKRTGEVGPIQVLGWERNKGGIRLTFIAGWRTLKLMGQQQQI
MKDVSKQLNSSEADIPAKVAQLLTSQKENEKAMQTMTEKLLYTEANELLQQPTKIHAGILISKVFTNRSMQEIAKLSAII
TEQQEHAITYFVIENEDKLQCILACGKTVTLDMNALLKEALPTIEGKGGGNKKSARGGGKAMMSGDEFLNQLISSLQSAV

Sequences:

>Translated_400_residues
MEQKLYYTDAYKQDFTTKVIKQDYDKDGNLYVILNETVFYPTGGGQPHDTGTLNGIAVLGVEEVDEEIRHFIAEQLHTEE
VEGKINWERRFDHMQQHAAQHILSAAFWDYFNIPTIGFHLGKETVTIDLETENLHAETVEKAVQIANKIVFENHPILIKW
MNLEEAKTLPLRKEPTMTENIRVVMIENFDYNGCGGTHPKRTGEVGPIQVLGWERNKGGIRLTFIAGWRTLKLMGQQQQI
MKDVSKQLNSSEADIPAKVAQLLTSQKENEKAMQTMTEKLLYTEANELLQQPTKIHAGILISKVFTNRSMQEIAKLSAII
TEQQEHAITYFVIENEDKLQCILACGKTVTLDMNALLKEALPTIEGKGGGNKKSARGGGKAMMSGDEFLNQLISSLQSAV
>Mature_400_residues
MEQKLYYTDAYKQDFTTKVIKQDYDKDGNLYVILNETVFYPTGGGQPHDTGTLNGIAVLGVEEVDEEIRHFIAEQLHTEE
VEGKINWERRFDHMQQHAAQHILSAAFWDYFNIPTIGFHLGKETVTIDLETENLHAETVEKAVQIANKIVFENHPILIKW
MNLEEAKTLPLRKEPTMTENIRVVMIENFDYNGCGGTHPKRTGEVGPIQVLGWERNKGGIRLTFIAGWRTLKLMGQQQQI
MKDVSKQLNSSEADIPAKVAQLLTSQKENEKAMQTMTEKLLYTEANELLQQPTKIHAGILISKVFTNRSMQEIAKLSAII
TEQQEHAITYFVIENEDKLQCILACGKTVTLDMNALLKEALPTIEGKGGGNKKSARGGGKAMMSGDEFLNQLISSLQSAV

Specific function: Functions in trans to edit the amino acid moiety from mischarged charged tRNA(Ala) [H]

COG id: COG2872

COG function: function code R; Predicted metal-dependent hydrolases related to alanyl-tRNA synthetase HxxxH domain

Gene ontology:

Cell location: Cytoplasm (Probable) [H]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the class-II aminoacyl-tRNA synthetase family. Editing domain AlaX-L subfamily [H]

Homologues:

Organism=Homo sapiens, GI13376886, Length=376, Percent_Identity=30.8510638297872, Blast_Score=141, Evalue=1e-33,
Organism=Homo sapiens, GI217416402, Length=376, Percent_Identity=30.8510638297872, Blast_Score=140, Evalue=1e-33,
Organism=Escherichia coli, GI1789048, Length=365, Percent_Identity=23.8356164383562, Blast_Score=89, Evalue=7e-19,
Organism=Saccharomyces cerevisiae, GI6324288, Length=293, Percent_Identity=24.5733788395904, Blast_Score=84, Evalue=5e-17,
Organism=Drosophila melanogaster, GI18543305, Length=396, Percent_Identity=26.2626262626263, Blast_Score=119, Evalue=4e-27,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR018165
- InterPro:   IPR018164
- InterPro:   IPR003156
- InterPro:   IPR018163
- InterPro:   IPR012947 [H]

Pfam domain/function: PF02272 DHHA1; PF01411 tRNA-synt_2c; PF07973 tRNA_SAD [H]

EC number: 6.1.1.7 [C]

Molecular weight: Translated: 45064; Mature: 45064

Theoretical pI: Translated: 5.62; Mature: 5.62

Prosite motif: PS50860 AA_TRNA_LIGASE_II_ALA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
3.2 %Met     (Translated Protein)
4.0 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
3.2 %Met     (Mature Protein)
4.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MEQKLYYTDAYKQDFTTKVIKQDYDKDGNLYVILNETVFYPTGGGQPHDTGTLNGIAVLG
CCCCEEECCHHHHHHHHHHHHHHCCCCCCEEEEEECEEEEECCCCCCCCCCCCCCEEEEE
VEEVDEEIRHFIAEQLHTEEVEGKINWERRFDHMQQHAAQHILSAAFWDYFNIPTIGFHL
HHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEEE
GKETVTIDLETENLHAETVEKAVQIANKIVFENHPILIKWMNLEEAKTLPLRKEPTMTEN
CCEEEEEEEECCCCCHHHHHHHHHHHHHHHCCCCCEEEEEECCCCCCCCCCCCCCCCCCC
IRVVMIENFDYNGCGGTHPKRTGEVGPIQVLGWERNKGGIRLTFIAGWRTLKLMGQQQQI
EEEEEEECCCCCCCCCCCCCCCCCCCCEEEEEEECCCCCEEEEEEECCHHHHHHCHHHHH
MKDVSKQLNSSEADIPAKVAQLLTSQKENEKAMQTMTEKLLYTEANELLQQPTKIHAGIL
HHHHHHHCCCCCCCCHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHH
ISKVFTNRSMQEIAKLSAIITEQQEHAITYFVIENEDKLQCILACGKTVTLDMNALLKEA
HHHHHCCCHHHHHHHHHHHHHCCCCCEEEEEEEECCCCEEEEEECCCEEEEEHHHHHHHH
LPTIEGKGGGNKKSARGGGKAMMSGDEFLNQLISSLQSAV
CCCCCCCCCCCCCCCCCCCCEEECHHHHHHHHHHHHHHCC
>Mature Secondary Structure
MEQKLYYTDAYKQDFTTKVIKQDYDKDGNLYVILNETVFYPTGGGQPHDTGTLNGIAVLG
CCCCEEECCHHHHHHHHHHHHHHCCCCCCEEEEEECEEEEECCCCCCCCCCCCCCEEEEE
VEEVDEEIRHFIAEQLHTEEVEGKINWERRFDHMQQHAAQHILSAAFWDYFNIPTIGFHL
HHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEEE
GKETVTIDLETENLHAETVEKAVQIANKIVFENHPILIKWMNLEEAKTLPLRKEPTMTEN
CCEEEEEEEECCCCCHHHHHHHHHHHHHHHCCCCCEEEEEECCCCCCCCCCCCCCCCCCC
IRVVMIENFDYNGCGGTHPKRTGEVGPIQVLGWERNKGGIRLTFIAGWRTLKLMGQQQQI
EEEEEEECCCCCCCCCCCCCCCCCCCCEEEEEEECCCCCEEEEEEECCHHHHHHCHHHHH
MKDVSKQLNSSEADIPAKVAQLLTSQKENEKAMQTMTEKLLYTEANELLQQPTKIHAGIL
HHHHHHHCCCCCCCCHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHH
ISKVFTNRSMQEIAKLSAIITEQQEHAITYFVIENEDKLQCILACGKTVTLDMNALLKEA
HHHHHCCCHHHHHHHHHHHHHCCCCCEEEEEEEECCCCEEEEEECCCEEEEEHHHHHHHH
LPTIEGKGGGNKKSARGGGKAMMSGDEFLNQLISSLQSAV
CCCCCCCCCCCCCCCCCCCCEEECHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: ATP; @ALAN01.txt*L-alanine!; tRNAAla [C]

Specific reaction: ATP + @ALAN01.txt*L-alanine! + tRNAAla = AMP + diphosphate + L-alanyl-tRNAAla [C]

General reaction: Aminoacylation; Esterification [C]

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 9679194 [H]