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The map label for this gene is yfkO [H]

Identifier: 218904471

GI number: 218904471

Start: 3201307

End: 3201987

Strand: Direct

Name: yfkO [H]

Synonym: BCAH820_3355

Alternate gene names: 218904471

Gene position: 3201307-3201987 (Clockwise)

Preceding gene: 218904469

Following gene: 218904472

Centisome position: 60.37

GC content: 32.45

Gene sequence:

>681_bases
ATGACAAATACAAATCAAATTACAAAAGAAAAAATTATGGAGGCTTTTCATTTCAGACATGCATGTAAAGAGTTCGATTC
TATGCAAAAAATTTCAGAAGAGGATTTTAAATTTATTTTAGAAACAGGAAGATTATCTCCTTCTTCTTTTGGATATGAAC
CTTGGAAATTTGTTGTCGTACAAAATAAAGAAGTAAGAGAAAAACTACGACCATATTCATGGGGCGCTGGTGGGCAACTT
GCAACAGCTAGCCACTTTGTCATTGTTCTTTCAAGAAATATTAAAGATATGCATTATGATGCAGAGTATATTAAACATAT
GATGAACGATATTATTGAATTACCTGAAGACGCGCAAAAAATTAGATATGAATTCTTTAAAAAGTTTCAGGAAACAGATT
TTAACTTATTACAATCCGACCGTGCTGTATTTGATTGGGCATCAAAGCAAACTTATATTGCTTTAGGGAATATGATGACG
AGTGCAGCTCAAATTGGCATTGATTCTTGTCCAATAGAAGGATTTGATAAAGAGAAAGTAGATTCTTTACTTCGTCAAGA
AGGCATTATTAAAGAAGATAATTTTGAAGTATCTGTAATGGTTGCGTTCGGTTACCGTAAAGAAGAGCCAAAGCGTGATA
AAACAAGACAGACTATGGATACAATTGTTGAATGGATTTAA

Upstream 100 bases:

>100_bases
CTTTTTTGATACTATACCACATAAATGTGCGTACTTGTTTCTATGTTACTTTAAAAATATAATTTGGTTACAAAATACAT
TAATGAAAAGAGGAACTTTT

Downstream 100 bases:

>100_bases
TAAGATAACATGCGCTCCTTATAGCTGTATACTCATACGAAAGAAGTAGAACTTCATTTTTCTTATGTATGAAATTAGTT
TTTCATGGAAAGATATATTA

Product: nitroreductase family protein

Products: NAD(P)(+); @TETR01.txt*5,6,7,8-Tetrahydropteridine!. [C]

Alternate protein names: NA

Number of amino acids: Translated: 226; Mature: 225

Protein sequence:

>226_residues
MTNTNQITKEKIMEAFHFRHACKEFDSMQKISEEDFKFILETGRLSPSSFGYEPWKFVVVQNKEVREKLRPYSWGAGGQL
ATASHFVIVLSRNIKDMHYDAEYIKHMMNDIIELPEDAQKIRYEFFKKFQETDFNLLQSDRAVFDWASKQTYIALGNMMT
SAAQIGIDSCPIEGFDKEKVDSLLRQEGIIKEDNFEVSVMVAFGYRKEEPKRDKTRQTMDTIVEWI

Sequences:

>Translated_226_residues
MTNTNQITKEKIMEAFHFRHACKEFDSMQKISEEDFKFILETGRLSPSSFGYEPWKFVVVQNKEVREKLRPYSWGAGGQL
ATASHFVIVLSRNIKDMHYDAEYIKHMMNDIIELPEDAQKIRYEFFKKFQETDFNLLQSDRAVFDWASKQTYIALGNMMT
SAAQIGIDSCPIEGFDKEKVDSLLRQEGIIKEDNFEVSVMVAFGYRKEEPKRDKTRQTMDTIVEWI
>Mature_225_residues
TNTNQITKEKIMEAFHFRHACKEFDSMQKISEEDFKFILETGRLSPSSFGYEPWKFVVVQNKEVREKLRPYSWGAGGQLA
TASHFVIVLSRNIKDMHYDAEYIKHMMNDIIELPEDAQKIRYEFFKKFQETDFNLLQSDRAVFDWASKQTYIALGNMMTS
AAQIGIDSCPIEGFDKEKVDSLLRQEGIIKEDNFEVSVMVAFGYRKEEPKRDKTRQTMDTIVEWI

Specific function: Reduction Of A Variety Of Nitroaromatic Compounds Using NADH (And To Lesser Extent Nadph) As Source Of Reducing Equivalents; Two Electrons Are Transferred. Capable Of Reducing Nitrofurazone, Quinones And The Anti-Tumor Agent Cb1954 (5- (Aziridin-1-Yl)-2,4

COG id: COG0778

COG function: function code C; Nitroreductase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the nitroreductase family [H]

Homologues:

Organism=Escherichia coli, GI1786792, Length=200, Percent_Identity=29, Blast_Score=72, Evalue=4e-14,

Paralogues:

None

Copy number: 260 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 20 Molecules/Cell In: Stationary Phase, Rich Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000415 [H]

Pfam domain/function: PF00881 Nitroreductase [H]

EC number: 1.-.-.- [C]

Molecular weight: Translated: 26515; Mature: 26383

Theoretical pI: Translated: 5.44; Mature: 5.44

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
4.4 %Met     (Translated Protein)
5.3 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
4.0 %Met     (Mature Protein)
4.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTNTNQITKEKIMEAFHFRHACKEFDSMQKISEEDFKFILETGRLSPSSFGYEPWKFVVV
CCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCEEEEEE
QNKEVREKLRPYSWGAGGQLATASHFVIVLSRNIKDMHYDAEYIKHMMNDIIELPEDAQK
CCHHHHHHCCCCCCCCCCCCCCCCEEEEEEECCCCHHHCCHHHHHHHHHHHHHCCHHHHH
IRYEFFKKFQETDFNLLQSDRAVFDWASKQTYIALGNMMTSAAQIGIDSCPIEGFDKEKV
HHHHHHHHHHHCCHHHHHHCHHHHHHCCCCCEEHHHHHHHHHHHHCCCCCCCCCCCHHHH
DSLLRQEGIIKEDNFEVSVMVAFGYRKEEPKRDKTRQTMDTIVEWI
HHHHHHCCCCCCCCCEEEEEEEECCCCCCCCHHHHHHHHHHHHHCC
>Mature Secondary Structure 
TNTNQITKEKIMEAFHFRHACKEFDSMQKISEEDFKFILETGRLSPSSFGYEPWKFVVV
CCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCEEEEEE
QNKEVREKLRPYSWGAGGQLATASHFVIVLSRNIKDMHYDAEYIKHMMNDIIELPEDAQK
CCHHHHHHCCCCCCCCCCCCCCCCEEEEEEECCCCHHHCCHHHHHHHHHHHHHCCHHHHH
IRYEFFKKFQETDFNLLQSDRAVFDWASKQTYIALGNMMTSAAQIGIDSCPIEGFDKEKV
HHHHHHHHHHHCCHHHHHHCHHHHHHCCCCCEEHHHHHHHHHHHHCCCCCCCCCCCHHHH
DSLLRQEGIIKEDNFEVSVMVAFGYRKEEPKRDKTRQTMDTIVEWI
HHHHHHCCCCCCCCCEEEEEEEECCCCCCCCHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: FMN. [C]

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NAD(P)H; 6,7-Dihydropteridine [C]

Specific reaction: NAD(P)H + 6,7-Dihydropteridine = NAD(P)(+) + @TETR01.txt*5,6,7,8-Tetrahydropteridine!. [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9384377 [H]