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The map label for this gene is vanSB [H]

Identifier: 218904324

GI number: 218904324

Start: 3044663

End: 3046006

Strand: Reverse

Name: vanSB [H]

Synonym: BCAH820_3208

Alternate gene names: 218904324

Gene position: 3046006-3044663 (Counterclockwise)

Preceding gene: 218904325

Following gene: 218904323

Centisome position: 57.44

GC content: 42.34

Gene sequence:

>1344_bases
ATGAAAAAGAATGGGATATTTATTAAGATTTTTGCCTATACGATAGTTGCTATGTTGTTGATTGTTTTCGTAACTACCGC
ATTGTTTTCAGGTCAATTCTTAACACTGTACAGAGCAGTTGAAAGGGAACAAATATTAGTGTCCTATCAGCCTTTGGTCA
ATAGAGTAAAAAATAGTGATTATAACGATATTCCCCTAGCAGCGCAAAGGTTTCGTGACAAAAACCAATCATTTGAGTTT
TGCATTATAGATGATAATGGCGGTGTGATTTACGCTACGCCGGGTGCTGATACCTCGGATAATTTCAAGGGTGACTTTTA
CTATGTGGTGCATAATGATAAAGCCCTCGGCTATTCTGTCGTCGCCCAGACCAGGCCGGGCATAACTGCCTTTTACAATG
AAATAATTGTGCGAGCATTAGTTGCGTTTGCCATTATACTCACCCTCTGCCTTGTGTGCGCGTATGTTTTTGCACGGCAG
ATAACCAAACCCATCAAGCGACTGGCGGATGACGCCGGAAAAATGACAAGGCTTGAAGATGTACCGCAAAGCCTGCCTAT
ACGCCGGGATGAATTAGGCGATTTGTCACGTGATATTCACTCTATGTACGTTAAGTTGAAAGATACCATTTCTCAGTTAG
AAAATGAAATCCTGCGGGTAAGGGAAATGGAGGAAGCACAGCGATATTTTTTTTCGGCGGCTTCACATGAGCTAAAAACA
CCTATCGCGGCAACAAGTATCTTGCTGGAGGGTATGCTCGAAAATGTAGGTGATTACAAAGACCATCCAAAATACTTGCG
CGAATGCATGAAGATGATGGACGCGCAGGACAATGTGATTTCCGAGATACTGGAGATTGTCAACCTGAGCGATGGGAAAA
TCTCTCAGGATTCTGAAAAACTGAAAGTCAGGAAAATAGTGGCTGATATACTGCCTGACTTTCAGATATTAGCTGAAGCA
AACGGTCAGTGCATTGTTATGGACATTCCTGACGGACAAAACTGCCTTGTCGATTCTAAAATGCTTCAAAAAGTACTTTC
TAATATCATTTTGAACGCAGTACAGAATACGCCTCCAGGCGGTGAAATTAGGATATGGAGCGAGCTTGTGGATAAACAAT
ATCGCCTTTGCATACTAAACACAGATGCAAGGATAGACGATACAGTTCTGCCTAAGTTATTTGACCCATTCTACCGTATG
GATAAAGTGCGAGGGCGGAAAAGTGGGCGCAACGGTTTAGGCTTGACCATCGTGCGGAAAACGTTGGAAGTAATGAACAT
TGAATATGCTTTGGAAAACGTTCCGGATGGCGTTCTATTTTGGATGGATTTGCCAAAGACATAA

Upstream 100 bases:

>100_bases
GCAGCACCGTGCATACTCACATTAAAAACCTACGGGCGAAGCTACCGGATAATATCATCAAAACAGTACGCGGAGTGGGC
TACCGATTGGAGGAAACTTC

Downstream 100 bases:

>100_bases
ATAATACAAGGAGAAAAAATAATGAAATCAGAAAACCGAAATAAACTTACAATTACATTGTTTGTAGTATATATGCTGCT
GTTGACAGGCGTCATTCTCT

Product: sensor protein vanSB

Products: NA

Alternate protein names: Vancomycin B-type resistance protein vanSB; Vancomycin histidine protein kinase [H]

Number of amino acids: Translated: 447; Mature: 447

Protein sequence:

>447_residues
MKKNGIFIKIFAYTIVAMLLIVFVTTALFSGQFLTLYRAVEREQILVSYQPLVNRVKNSDYNDIPLAAQRFRDKNQSFEF
CIIDDNGGVIYATPGADTSDNFKGDFYYVVHNDKALGYSVVAQTRPGITAFYNEIIVRALVAFAIILTLCLVCAYVFARQ
ITKPIKRLADDAGKMTRLEDVPQSLPIRRDELGDLSRDIHSMYVKLKDTISQLENEILRVREMEEAQRYFFSAASHELKT
PIAATSILLEGMLENVGDYKDHPKYLRECMKMMDAQDNVISEILEIVNLSDGKISQDSEKLKVRKIVADILPDFQILAEA
NGQCIVMDIPDGQNCLVDSKMLQKVLSNIILNAVQNTPPGGEIRIWSELVDKQYRLCILNTDARIDDTVLPKLFDPFYRM
DKVRGRKSGRNGLGLTIVRKTLEVMNIEYALENVPDGVLFWMDLPKT

Sequences:

>Translated_447_residues
MKKNGIFIKIFAYTIVAMLLIVFVTTALFSGQFLTLYRAVEREQILVSYQPLVNRVKNSDYNDIPLAAQRFRDKNQSFEF
CIIDDNGGVIYATPGADTSDNFKGDFYYVVHNDKALGYSVVAQTRPGITAFYNEIIVRALVAFAIILTLCLVCAYVFARQ
ITKPIKRLADDAGKMTRLEDVPQSLPIRRDELGDLSRDIHSMYVKLKDTISQLENEILRVREMEEAQRYFFSAASHELKT
PIAATSILLEGMLENVGDYKDHPKYLRECMKMMDAQDNVISEILEIVNLSDGKISQDSEKLKVRKIVADILPDFQILAEA
NGQCIVMDIPDGQNCLVDSKMLQKVLSNIILNAVQNTPPGGEIRIWSELVDKQYRLCILNTDARIDDTVLPKLFDPFYRM
DKVRGRKSGRNGLGLTIVRKTLEVMNIEYALENVPDGVLFWMDLPKT
>Mature_447_residues
MKKNGIFIKIFAYTIVAMLLIVFVTTALFSGQFLTLYRAVEREQILVSYQPLVNRVKNSDYNDIPLAAQRFRDKNQSFEF
CIIDDNGGVIYATPGADTSDNFKGDFYYVVHNDKALGYSVVAQTRPGITAFYNEIIVRALVAFAIILTLCLVCAYVFARQ
ITKPIKRLADDAGKMTRLEDVPQSLPIRRDELGDLSRDIHSMYVKLKDTISQLENEILRVREMEEAQRYFFSAASHELKT
PIAATSILLEGMLENVGDYKDHPKYLRECMKMMDAQDNVISEILEIVNLSDGKISQDSEKLKVRKIVADILPDFQILAEA
NGQCIVMDIPDGQNCLVDSKMLQKVLSNIILNAVQNTPPGGEIRIWSELVDKQYRLCILNTDARIDDTVLPKLFDPFYRM
DKVRGRKSGRNGLGLTIVRKTLEVMNIEYALENVPDGVLFWMDLPKT

Specific function: Member of the two-component regulatory system vanS/vanR. Activates the transcription of vanH, vanA and vanX in response to vancomycin which results in vancomycin resistance. VanS activates vanR by phosphorylation [H]

COG id: COG0642

COG function: function code T; Signal transduction histidine kinase

Gene ontology:

Cell location: Cell membrane; Multi-pass membrane protein (Potential) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 histidine kinase domain [H]

Homologues:

Organism=Escherichia coli, GI1788393, Length=295, Percent_Identity=27.4576271186441, Blast_Score=99, Evalue=5e-22,
Organism=Escherichia coli, GI1786600, Length=207, Percent_Identity=26.0869565217391, Blast_Score=88, Evalue=9e-19,
Organism=Escherichia coli, GI1786783, Length=254, Percent_Identity=25.9842519685039, Blast_Score=86, Evalue=5e-18,
Organism=Escherichia coli, GI1790861, Length=311, Percent_Identity=27.9742765273312, Blast_Score=83, Evalue=3e-17,
Organism=Escherichia coli, GI1790346, Length=229, Percent_Identity=26.2008733624454, Blast_Score=70, Evalue=3e-13,
Organism=Escherichia coli, GI1790551, Length=321, Percent_Identity=23.0529595015576, Blast_Score=69, Evalue=8e-13,
Organism=Escherichia coli, GI1789149, Length=352, Percent_Identity=22.7272727272727, Blast_Score=68, Evalue=1e-12,
Organism=Escherichia coli, GI1788549, Length=238, Percent_Identity=27.3109243697479, Blast_Score=67, Evalue=2e-12,
Organism=Escherichia coli, GI87081816, Length=342, Percent_Identity=22.5146198830409, Blast_Score=64, Evalue=2e-11,
Organism=Escherichia coli, GI87082128, Length=313, Percent_Identity=24.6006389776358, Blast_Score=64, Evalue=3e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003594
- InterPro:   IPR003660
- InterPro:   IPR003661
- InterPro:   IPR005467
- InterPro:   IPR009082 [H]

Pfam domain/function: PF00672 HAMP; PF02518 HATPase_c; PF00512 HisKA [H]

EC number: =2.7.13.3 [H]

Molecular weight: Translated: 50753; Mature: 50753

Theoretical pI: Translated: 5.59; Mature: 5.59

Prosite motif: PS50885 HAMP ; PS50109 HIS_KIN

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.6 %Cys     (Translated Protein)
3.1 %Met     (Translated Protein)
4.7 %Cys+Met (Translated Protein)
1.6 %Cys     (Mature Protein)
3.1 %Met     (Mature Protein)
4.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKKNGIFIKIFAYTIVAMLLIVFVTTALFSGQFLTLYRAVEREQILVSYQPLVNRVKNSD
CCCCCEEEEEHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCC
YNDIPLAAQRFRDKNQSFEFCIIDDNGGVIYATPGADTSDNFKGDFYYVVHNDKALGYSV
CCCCCHHHHHHHCCCCCEEEEEEECCCCEEEECCCCCCCCCCCCCEEEEEECCCCCCCEE
VAQTRPGITAFYNEIIVRALVAFAIILTLCLVCAYVFARQITKPIKRLADDAGKMTRLED
EECCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
VPQSLPIRRDELGDLSRDIHSMYVKLKDTISQLENEILRVREMEEAQRYFFSAASHELKT
CHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHC
PIAATSILLEGMLENVGDYKDHPKYLRECMKMMDAQDNVISEILEIVNLSDGKISQDSEK
HHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHCCCHHHHHHHHHHHCCCCCCCCCCHHH
LKVRKIVADILPDFQILAEANGQCIVMDIPDGQNCLVDSKMLQKVLSNIILNAVQNTPPG
HHHHHHHHHHCCCHHHHHCCCCCEEEEECCCCCCCCCCHHHHHHHHHHHHHHHHCCCCCC
GEIRIWSELVDKQYRLCILNTDARIDDTVLPKLFDPFYRMDKVRGRKSGRNGLGLTIVRK
CCEEEHHHHHCCCEEEEEEECCCCCCHHHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHH
TLEVMNIEYALENVPDGVLFWMDLPKT
HHHHHHHHHHHHCCCCCEEEEEECCCC
>Mature Secondary Structure
MKKNGIFIKIFAYTIVAMLLIVFVTTALFSGQFLTLYRAVEREQILVSYQPLVNRVKNSD
CCCCCEEEEEHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCC
YNDIPLAAQRFRDKNQSFEFCIIDDNGGVIYATPGADTSDNFKGDFYYVVHNDKALGYSV
CCCCCHHHHHHHCCCCCEEEEEEECCCCEEEECCCCCCCCCCCCCEEEEEECCCCCCCEE
VAQTRPGITAFYNEIIVRALVAFAIILTLCLVCAYVFARQITKPIKRLADDAGKMTRLED
EECCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
VPQSLPIRRDELGDLSRDIHSMYVKLKDTISQLENEILRVREMEEAQRYFFSAASHELKT
CHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHC
PIAATSILLEGMLENVGDYKDHPKYLRECMKMMDAQDNVISEILEIVNLSDGKISQDSEK
HHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHCCCHHHHHHHHHHHCCCCCCCCCCHHH
LKVRKIVADILPDFQILAEANGQCIVMDIPDGQNCLVDSKMLQKVLSNIILNAVQNTPPG
HHHHHHHHHHCCCHHHHHCCCCCEEEEECCCCCCCCCCHHHHHHHHHHHHHHHHCCCCCC
GEIRIWSELVDKQYRLCILNTDARIDDTVLPKLFDPFYRMDKVRGRKSGRNGLGLTIVRK
CCEEEHHHHHCCCEEEEEEECCCCCCHHHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHH
TLEVMNIEYALENVPDGVLFWMDLPKT
HHHHHHHHHHHHCCCCCEEEEEECCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 8631706; 12663927 [H]