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The map label for this gene is bmr3 [H]

Identifier: 218903996

GI number: 218903996

Start: 2728956

End: 2730491

Strand: Reverse

Name: bmr3 [H]

Synonym: BCAH820_2880

Alternate gene names: 218903996

Gene position: 2730491-2728956 (Counterclockwise)

Preceding gene: 218903997

Following gene: 218903994

Centisome position: 51.49

GC content: 36.91

Gene sequence:

>1536_bases
ATGGTTGAGAAGAATAATAAGCTCGGCTTTGTTGTGGCGGGCTTATTGCTAGGTATTTTAATGGCATCAATGGATAATAC
CATTGTCGTAACAGCGATGGGAACGATTGTTGGTGACTTAGGAGGTCTTGAAAACTTTGTATGGGTCGTTTCGGCCTATA
TGGTCGCAGAAATGGCAGGTATGCCGATTTTCGGTAAACTATCAGATATGTATGGTAGAAAGAGATTCTTTATTTTTGGT
TTAATCGTCTTTATGGTTGGTTCGGCACTTTGTGGTACTGCTGAAAATATTACACAGTTAGGTATTTATCGTGCCATTCA
AGGTATTGGTGGCGGGGCATTAGTGCCGATCGCGTTTACAATCGTTTTTGATATTTTCCCTCCTGAAAAGCGCGGGAAAA
TGGGTGGTTTATTTGGAGCGGTATTTGGTTTATCAAGTATTTTCGGGCCATTACTTGGCGCATATATTACAGATTACATT
AGCTGGCACTGGGTATTTTATATTAACTTACCGCTTGGCGTTTTAGCACTTATTTTTATTACGTTCTTTTATAAAGAATC
AAGAGTTCATAGAAAGCAAAAAATTGATTGGTTTGGTGCAATTACTTTAGTTGGTGCAGTAATTTGTTTAATGTTTGCGC
TTGAACTTGGTGGACAAAAATATGATTGGGATTCTACCTTTATTTTAAGTTTATTTGTTGGATTTGCTATTTTAATTATT
TCATTTATTTTTATTGAACGTAAAGTAGAAGAACCGATCATTTCATTTGAGATGTTTAAGCAGCGCTTATTCGGAATGAG
TACCATTATTGCTTTATGTTACGGTGCTGCGTTTATGTCGGCAACTGTATATATTCCGTTATTCATTCAAGGTGTATACG
GAGGTAGTGCAACAAACTCAGGGTTATTACTTTTACCGATGATGTTAGGATCAGTTGTAACAGCGCAGTTAGGCGGATTT
TTAACGACGAAGCTTAGCTACCGAAACATTATGATTATTTCTGCGGTTATTATGCTAATTGGACTATTTTTATTAAGCAC
ATTAACGCCAGAAACAAGTCGCGCATTATTAACAGTGTATATGATTATTATCGGATTTGGAGTTGGATTCTCATTTTCTG
TACTAAGTATGGCAGCTATTCACAACTTTGGTATGGAGCAGCGTGGATCTGCGACTTCAACGAGTAATTTCATTCGTTCA
TTAGGAATGACGCTTGGTATTACAATCTTCGGAATGATTCAAAGAACTGGCTTCCAAGATCAATTAGAAGAAGTATTTAA
AGGTATGAGCGGGGGAATGAATACAAACGCTTTAGGAGATTCAAGAGCTATTCTATCAGAATCGGCAAGATCTCAAATCC
CGCCGCAAATATTAGATAAAATTATTGACGCACTTTCTAGCTCAATTGTCCAAACATTTATGTGGGCATTGGTACCAGCT
GGTTTAGCATTCATATTTATTTTCTTTATGGGGAATGAGCGCATGGTAATGAAGAAACAACAAAAGAATAAAAAAGGCGA
AATATCAAAAGCGTAA

Upstream 100 bases:

>100_bases
AATTAGTGTTGTAGAATATCATACTAGTTTTAAGCAAAAAGGTAATGCAGTCGTAAAAGAACATAAACAAATATAAGTAG
TATGAGGGAGAGCAAGAGAT

Downstream 100 bases:

>100_bases
TGAAAAAGCTCCTTTCCAATTGGAAGGGAGCTTTTTCGTATGTACGGATTTAGATTAGCCTTCGATGATTTCGTATTCTT
CGTCTGCTAATAATAAAATT

Product: drug resistance transporter, EmrB/QacA family

Products: Proton [Cytoplasm]; multidrug [Periplasm] [C]

Alternate protein names: Multidrug-efflux transporter 3 [H]

Number of amino acids: Translated: 511; Mature: 511

Protein sequence:

>511_residues
MVEKNNKLGFVVAGLLLGILMASMDNTIVVTAMGTIVGDLGGLENFVWVVSAYMVAEMAGMPIFGKLSDMYGRKRFFIFG
LIVFMVGSALCGTAENITQLGIYRAIQGIGGGALVPIAFTIVFDIFPPEKRGKMGGLFGAVFGLSSIFGPLLGAYITDYI
SWHWVFYINLPLGVLALIFITFFYKESRVHRKQKIDWFGAITLVGAVICLMFALELGGQKYDWDSTFILSLFVGFAILII
SFIFIERKVEEPIISFEMFKQRLFGMSTIIALCYGAAFMSATVYIPLFIQGVYGGSATNSGLLLLPMMLGSVVTAQLGGF
LTTKLSYRNIMIISAVIMLIGLFLLSTLTPETSRALLTVYMIIIGFGVGFSFSVLSMAAIHNFGMEQRGSATSTSNFIRS
LGMTLGITIFGMIQRTGFQDQLEEVFKGMSGGMNTNALGDSRAILSESARSQIPPQILDKIIDALSSSIVQTFMWALVPA
GLAFIFIFFMGNERMVMKKQQKNKKGEISKA

Sequences:

>Translated_511_residues
MVEKNNKLGFVVAGLLLGILMASMDNTIVVTAMGTIVGDLGGLENFVWVVSAYMVAEMAGMPIFGKLSDMYGRKRFFIFG
LIVFMVGSALCGTAENITQLGIYRAIQGIGGGALVPIAFTIVFDIFPPEKRGKMGGLFGAVFGLSSIFGPLLGAYITDYI
SWHWVFYINLPLGVLALIFITFFYKESRVHRKQKIDWFGAITLVGAVICLMFALELGGQKYDWDSTFILSLFVGFAILII
SFIFIERKVEEPIISFEMFKQRLFGMSTIIALCYGAAFMSATVYIPLFIQGVYGGSATNSGLLLLPMMLGSVVTAQLGGF
LTTKLSYRNIMIISAVIMLIGLFLLSTLTPETSRALLTVYMIIIGFGVGFSFSVLSMAAIHNFGMEQRGSATSTSNFIRS
LGMTLGITIFGMIQRTGFQDQLEEVFKGMSGGMNTNALGDSRAILSESARSQIPPQILDKIIDALSSSIVQTFMWALVPA
GLAFIFIFFMGNERMVMKKQQKNKKGEISKA
>Mature_511_residues
MVEKNNKLGFVVAGLLLGILMASMDNTIVVTAMGTIVGDLGGLENFVWVVSAYMVAEMAGMPIFGKLSDMYGRKRFFIFG
LIVFMVGSALCGTAENITQLGIYRAIQGIGGGALVPIAFTIVFDIFPPEKRGKMGGLFGAVFGLSSIFGPLLGAYITDYI
SWHWVFYINLPLGVLALIFITFFYKESRVHRKQKIDWFGAITLVGAVICLMFALELGGQKYDWDSTFILSLFVGFAILII
SFIFIERKVEEPIISFEMFKQRLFGMSTIIALCYGAAFMSATVYIPLFIQGVYGGSATNSGLLLLPMMLGSVVTAQLGGF
LTTKLSYRNIMIISAVIMLIGLFLLSTLTPETSRALLTVYMIIIGFGVGFSFSVLSMAAIHNFGMEQRGSATSTSNFIRS
LGMTLGITIFGMIQRTGFQDQLEEVFKGMSGGMNTNALGDSRAILSESARSQIPPQILDKIIDALSSSIVQTFMWALVPA
GLAFIFIFFMGNERMVMKKQQKNKKGEISKA

Specific function: Confers resistance to puromycin, tosufloxacin and norfloxacin [H]

COG id: COG0477

COG function: function code GEPR; Permeases of the major facilitator superfamily

Gene ontology:

Cell location: Cell membrane; Multi-pass membrane protein (Potential) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the major facilitator superfamily. EmrB family [H]

Homologues:

Organism=Escherichia coli, GI1789042, Length=403, Percent_Identity=30.0248138957816, Blast_Score=168, Evalue=6e-43,
Organism=Escherichia coli, GI87081983, Length=401, Percent_Identity=29.426433915212, Blast_Score=157, Evalue=2e-39,
Organism=Escherichia coli, GI1788710, Length=441, Percent_Identity=28.5714285714286, Blast_Score=151, Evalue=1e-37,
Organism=Escherichia coli, GI1790195, Length=420, Percent_Identity=24.7619047619048, Blast_Score=135, Evalue=9e-33,
Organism=Escherichia coli, GI1788392, Length=413, Percent_Identity=24.6973365617433, Blast_Score=107, Evalue=1e-24,
Organism=Escherichia coli, GI1790146, Length=162, Percent_Identity=29.0123456790123, Blast_Score=72, Evalue=6e-14,
Organism=Escherichia coli, GI48994889, Length=134, Percent_Identity=31.3432835820896, Blast_Score=67, Evalue=2e-12,
Organism=Saccharomyces cerevisiae, GI6322958, Length=557, Percent_Identity=23.5188509874327, Blast_Score=143, Evalue=7e-35,
Organism=Saccharomyces cerevisiae, GI6319770, Length=466, Percent_Identity=25.9656652360515, Blast_Score=130, Evalue=6e-31,
Organism=Saccharomyces cerevisiae, GI6319783, Length=460, Percent_Identity=23.695652173913, Blast_Score=117, Evalue=4e-27,
Organism=Saccharomyces cerevisiae, GI6325455, Length=529, Percent_Identity=22.8733459357278, Blast_Score=111, Evalue=2e-25,
Organism=Saccharomyces cerevisiae, GI6323735, Length=322, Percent_Identity=30.1242236024845, Blast_Score=110, Evalue=4e-25,
Organism=Saccharomyces cerevisiae, GI6321663, Length=540, Percent_Identity=22.5925925925926, Blast_Score=98, Evalue=3e-21,
Organism=Saccharomyces cerevisiae, GI6324954, Length=398, Percent_Identity=24.8743718592965, Blast_Score=90, Evalue=8e-19,
Organism=Saccharomyces cerevisiae, GI6323520, Length=410, Percent_Identity=24.390243902439, Blast_Score=68, Evalue=3e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004638
- InterPro:   IPR020846
- InterPro:   IPR011701
- InterPro:   IPR016196 [H]

Pfam domain/function: PF07690 MFS_1 [H]

EC number: NA

Molecular weight: Translated: 55829; Mature: 55829

Theoretical pI: Translated: 9.47; Mature: 9.47

Prosite motif: PS50850 MFS

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
5.7 %Met     (Translated Protein)
6.3 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
5.7 %Met     (Mature Protein)
6.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MVEKNNKLGFVVAGLLLGILMASMDNTIVVTAMGTIVGDLGGLENFVWVVSAYMVAEMAG
CCCCCCCHHHHHHHHHHHHHHHCCCCCEEEEEHHHHHHHHCCHHHHHHHHHHHHHHHHHC
MPIFGKLSDMYGRKRFFIFGLIVFMVGSALCGTAENITQLGIYRAIQGIGGGALVPIAFT
CCCHHHHHHHHCCHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHCCCCCHHHHHHHH
IVFDIFPPEKRGKMGGLFGAVFGLSSIFGPLLGAYITDYISWHWVFYINLPLGVLALIFI
HHHHCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHEEEEEEEECCHHHHHHHHH
TFFYKESRVHRKQKIDWFGAITLVGAVICLMFALELGGQKYDWDSTFILSLFVGFAILII
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHH
SFIFIERKVEEPIISFEMFKQRLFGMSTIIALCYGAAFMSATVYIPLFIQGVYGGSATNS
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHEEECCCCCCCC
GLLLLPMMLGSVVTAQLGGFLTTKLSYRNIMIISAVIMLIGLFLLSTLTPETSRALLTVY
CHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHH
MIIIGFGVGFSFSVLSMAAIHNFGMEQRGSATSTSNFIRSLGMTLGITIFGMIQRTGFQD
HHHHHHCCCHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCHH
QLEEVFKGMSGGMNTNALGDSRAILSESARSQIPPQILDKIIDALSSSIVQTFMWALVPA
HHHHHHHHHCCCCCCCCCCCHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHH
GLAFIFIFFMGNERMVMKKQQKNKKGEISKA
HHHHHHHHHHCCCHHHHHHHHCCCCCCCCCC
>Mature Secondary Structure
MVEKNNKLGFVVAGLLLGILMASMDNTIVVTAMGTIVGDLGGLENFVWVVSAYMVAEMAG
CCCCCCCHHHHHHHHHHHHHHHCCCCCEEEEEHHHHHHHHCCHHHHHHHHHHHHHHHHHC
MPIFGKLSDMYGRKRFFIFGLIVFMVGSALCGTAENITQLGIYRAIQGIGGGALVPIAFT
CCCHHHHHHHHCCHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHCCCCCHHHHHHHH
IVFDIFPPEKRGKMGGLFGAVFGLSSIFGPLLGAYITDYISWHWVFYINLPLGVLALIFI
HHHHCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHEEEEEEEECCHHHHHHHHH
TFFYKESRVHRKQKIDWFGAITLVGAVICLMFALELGGQKYDWDSTFILSLFVGFAILII
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHH
SFIFIERKVEEPIISFEMFKQRLFGMSTIIALCYGAAFMSATVYIPLFIQGVYGGSATNS
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHEEECCCCCCCC
GLLLLPMMLGSVVTAQLGGFLTTKLSYRNIMIISAVIMLIGLFLLSTLTPETSRALLTVY
CHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHH
MIIIGFGVGFSFSVLSMAAIHNFGMEQRGSATSTSNFIRSLGMTLGITIFGMIQRTGFQD
HHHHHHCCCHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCHH
QLEEVFKGMSGGMNTNALGDSRAILSESARSQIPPQILDKIIDALSSSIVQTFMWALVPA
HHHHHHHHHCCCCCCCCCCCHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHH
GLAFIFIFFMGNERMVMKKQQKNKKGEISKA
HHHHHHHHHHCCCHHHHHHHHCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: Proton [Periplasm]; multidrug [Cytoplasm] [C]

Specific reaction: Proton [Periplasm] + multidrug [Cytoplasm] = Proton [Cytoplasm] + multidrug [Periplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 9023234; 9384377 [H]