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The map label for this gene is ooxB [H]

Identifier: 218903957

GI number: 218903957

Start: 2696566

End: 2697741

Strand: Reverse

Name: ooxB [H]

Synonym: BCAH820_2841

Alternate gene names: 218903957

Gene position: 2697741-2696566 (Counterclockwise)

Preceding gene: 218903962

Following gene: 218903956

Centisome position: 50.88

GC content: 39.8

Gene sequence:

>1176_bases
GTGAGGCACTGCGACGTTTTAATAATTGGCGGTGGTATTATTGGCTGCTCGATCGCTTATTACACTTCAAAATACGGAAG
AGACGTAACAATCATTGAAAAAGGAGAATTTGTCAGCGGGACGTCTTCACGCTGTGATGGAAATATTTTGGCTATTGATA
AAGACCCGGGGTTTGATAGTCAAATGTCGTTAGTAAGTCAAAAATTAGTAACGGATTTAAGTGAAGAGTTAGAGCACTCA
TTTGAATATAGGGCGCCAGGAAGTATTCTCGTATGTGAGTCAGACGAAGAAATGGAAGCAGCGCAGCAATGGGTGAATCG
TCAAAAAGAAGCGGGTTTACCGTTTCGAATGCTTGATAGGCAAGATATAAGAGCGGAATCGCCATTCTTTGCAGATGATT
TATTAGGCGGGTTAGAATGCGCAACAGATTCGACTGTAAATCCATATCTTCTTGCTTTTTCACTTCTTGCAGAATCGAAG
AAATATGGTACGAAGGCTTTTAATCATACGGAAGTAAAAGAAATGAAAAGAGATAAAGACGGTTCCTTTATTGTAGAAAC
GACAAATGAGACGTTTACTGCGAAGCAAGTGGTGAACGCAGCGGGTGTGTGGGCTCCGAAAATCGGACAAATGTTGGATG
TAAATATCCCCATTGAACCGAGAAAAGGGCATATTATTGTAGCTTCAAGGCAACAACACGTTGGTTGTCGTAAAGTTATG
GAATTTGGTTATTTAATTTCTAAATTTGGCGGAAAACGAAAAGTGGATGCTTTAACTGAAAAATATGGAGTAGCTCTCGT
ATTTGAGCCGACAGAAAGCCAAAATTTTTTAATTGGTAGTAGTAGAGAGTTTGTGGGGTTTCATACGAAGATAAACAACG
AGGTTATTAAATGTATTGCGAATAGAGCAATTCGTTTTTATCCAAAAATGGCGGATATGATGGTGATTCGTTCATATGCT
GGATTACGCCCGTGGACAGAAGATCATTTGCCGATTATTTCACGTGTGGAACATATCCCGAATTACTTTATTGCAGCAGG
GCATGAAGGGGATGGCATTAGTCTTGCCGCGGTTACAGGGAAAGTGATTGAAGAGTTATTAAATGAAAAAGAAACAATCA
TTCCTATTGAACCACTTCGTTTGAGTCGTTTTACAGAAAGGGTGTTAAACGGATGA

Upstream 100 bases:

>100_bases
TACAAATAAAAATATTGTTTGTAAAAATTGTATATATGTACAGTTGGCACGTCAATTGCATAAAAGAAAGTGAGAGGTTT
AGGGGAAGGAGGAGTATTTT

Downstream 100 bases:

>100_bases
GGACACAAAAAGTCTTTACGACGATTGATACACATACGGGTGGGAATCCAACGAGAACATTGATTAGCGGACTACCTAAG
TTACTTGGAGAGACGATGGC

Product: putative glycine oxidase

Products: glycine; formaldehyde; H2O2

Alternate protein names: Octopine oxidase subunit B [H]

Number of amino acids: Translated: 391; Mature: 391

Protein sequence:

>391_residues
MRHCDVLIIGGGIIGCSIAYYTSKYGRDVTIIEKGEFVSGTSSRCDGNILAIDKDPGFDSQMSLVSQKLVTDLSEELEHS
FEYRAPGSILVCESDEEMEAAQQWVNRQKEAGLPFRMLDRQDIRAESPFFADDLLGGLECATDSTVNPYLLAFSLLAESK
KYGTKAFNHTEVKEMKRDKDGSFIVETTNETFTAKQVVNAAGVWAPKIGQMLDVNIPIEPRKGHIIVASRQQHVGCRKVM
EFGYLISKFGGKRKVDALTEKYGVALVFEPTESQNFLIGSSREFVGFHTKINNEVIKCIANRAIRFYPKMADMMVIRSYA
GLRPWTEDHLPIISRVEHIPNYFIAAGHEGDGISLAAVTGKVIEELLNEKETIIPIEPLRLSRFTERVLNG

Sequences:

>Translated_391_residues
MRHCDVLIIGGGIIGCSIAYYTSKYGRDVTIIEKGEFVSGTSSRCDGNILAIDKDPGFDSQMSLVSQKLVTDLSEELEHS
FEYRAPGSILVCESDEEMEAAQQWVNRQKEAGLPFRMLDRQDIRAESPFFADDLLGGLECATDSTVNPYLLAFSLLAESK
KYGTKAFNHTEVKEMKRDKDGSFIVETTNETFTAKQVVNAAGVWAPKIGQMLDVNIPIEPRKGHIIVASRQQHVGCRKVM
EFGYLISKFGGKRKVDALTEKYGVALVFEPTESQNFLIGSSREFVGFHTKINNEVIKCIANRAIRFYPKMADMMVIRSYA
GLRPWTEDHLPIISRVEHIPNYFIAAGHEGDGISLAAVTGKVIEELLNEKETIIPIEPLRLSRFTERVLNG
>Mature_391_residues
MRHCDVLIIGGGIIGCSIAYYTSKYGRDVTIIEKGEFVSGTSSRCDGNILAIDKDPGFDSQMSLVSQKLVTDLSEELEHS
FEYRAPGSILVCESDEEMEAAQQWVNRQKEAGLPFRMLDRQDIRAESPFFADDLLGGLECATDSTVNPYLLAFSLLAESK
KYGTKAFNHTEVKEMKRDKDGSFIVETTNETFTAKQVVNAAGVWAPKIGQMLDVNIPIEPRKGHIIVASRQQHVGCRKVM
EFGYLISKFGGKRKVDALTEKYGVALVFEPTESQNFLIGSSREFVGFHTKINNEVIKCIANRAIRFYPKMADMMVIRSYA
GLRPWTEDHLPIISRVEHIPNYFIAAGHEGDGISLAAVTGKVIEELLNEKETIIPIEPLRLSRFTERVLNG

Specific function: Oxidative cleavage of octopine into L-arginine and pyruvate [H]

COG id: COG0665

COG function: function code E; Glycine/D-amino acid oxidases (deaminating)

Gene ontology:

Cell location: Inner Membrane-Bound [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: NA

Homologues:

Organism=Homo sapiens, GI24797151, Length=398, Percent_Identity=25.6281407035176, Blast_Score=114, Evalue=1e-25,
Organism=Homo sapiens, GI197927446, Length=378, Percent_Identity=21.6931216931217, Blast_Score=76, Evalue=5e-14,
Organism=Homo sapiens, GI21361378, Length=378, Percent_Identity=21.6931216931217, Blast_Score=76, Evalue=5e-14,
Organism=Homo sapiens, GI194306651, Length=209, Percent_Identity=25.8373205741627, Blast_Score=72, Evalue=6e-13,
Organism=Escherichia coli, GI1787438, Length=436, Percent_Identity=21.7889908256881, Blast_Score=94, Evalue=1e-20,
Organism=Escherichia coli, GI1787559, Length=380, Percent_Identity=20.5263157894737, Blast_Score=67, Evalue=2e-12,
Organism=Escherichia coli, GI1788574, Length=315, Percent_Identity=22.2222222222222, Blast_Score=65, Evalue=8e-12,
Organism=Caenorhabditis elegans, GI71994045, Length=228, Percent_Identity=25.8771929824561, Blast_Score=69, Evalue=5e-12,
Organism=Caenorhabditis elegans, GI71994052, Length=235, Percent_Identity=25.531914893617, Blast_Score=66, Evalue=3e-11,
Organism=Drosophila melanogaster, GI20130091, Length=390, Percent_Identity=19.7435897435897, Blast_Score=75, Evalue=8e-14,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR006076 [H]

Pfam domain/function: PF01266 DAO [H]

EC number: 1.5.3.1

Molecular weight: Translated: 43670; Mature: 43670

Theoretical pI: Translated: 5.88; Mature: 5.88

Prosite motif: PS00013 PROKAR_LIPOPROTEIN

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.8 %Cys     (Translated Protein)
2.6 %Met     (Translated Protein)
4.3 %Cys+Met (Translated Protein)
1.8 %Cys     (Mature Protein)
2.6 %Met     (Mature Protein)
4.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRHCDVLIIGGGIIGCSIAYYTSKYGRDVTIIEKGEFVSGTSSRCDGNILAIDKDPGFDS
CCCCCEEEECCCHHHHHHHHHHHHCCCEEEEEECCCCCCCCCCCCCCCEEEEECCCCCCH
QMSLVSQKLVTDLSEELEHSFEYRAPGSILVCESDEEMEAAQQWVNRQKEAGLPFRMLDR
HHHHHHHHHHHHHHHHHHHCCCCCCCCCEEEECCCHHHHHHHHHHHHHHHCCCCEEECCC
QDIRAESPFFADDLLGGLECATDSTVNPYLLAFSLLAESKKYGTKAFNHTEVKEMKRDKD
HHCCCCCCCCHHHHHCCCEECCCCCCCHHHHHHHHHHHHHHHCCCCCCHHHHHHHHCCCC
GSFIVETTNETFTAKQVVNAAGVWAPKIGQMLDVNIPIEPRKGHIIVASRQQHVGCRKVM
CCEEEEECCCCHHHHHHHHHCCCCCCCCCCEEEECCCCCCCCCEEEEECCCHHHHHHHHH
EFGYLISKFGGKRKVDALTEKYGVALVFEPTESQNFLIGSSREFVGFHTKINNEVIKCIA
HHHHHHHHCCCCCHHHHHHHHHCEEEEEECCCCCCEEEECCCCEEEEEEHHHHHHHHHHH
NRAIRFYPKMADMMVIRSYAGLRPWTEDHLPIISRVEHIPNYFIAAGHEGDGISLAAVTG
HHHHHHHHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHCCCEEEEECCCCCCEEEEHHHH
KVIEELLNEKETIIPIEPLRLSRFTERVLNG
HHHHHHHCCCCEEECCCCHHHHHHHHHHHCC
>Mature Secondary Structure
MRHCDVLIIGGGIIGCSIAYYTSKYGRDVTIIEKGEFVSGTSSRCDGNILAIDKDPGFDS
CCCCCEEEECCCHHHHHHHHHHHHCCCEEEEEECCCCCCCCCCCCCCCEEEEECCCCCCH
QMSLVSQKLVTDLSEELEHSFEYRAPGSILVCESDEEMEAAQQWVNRQKEAGLPFRMLDR
HHHHHHHHHHHHHHHHHHHCCCCCCCCCEEEECCCHHHHHHHHHHHHHHHCCCCEEECCC
QDIRAESPFFADDLLGGLECATDSTVNPYLLAFSLLAESKKYGTKAFNHTEVKEMKRDKD
HHCCCCCCCCHHHHHCCCEECCCCCCCHHHHHHHHHHHHHHHCCCCCCHHHHHHHHCCCC
GSFIVETTNETFTAKQVVNAAGVWAPKIGQMLDVNIPIEPRKGHIIVASRQQHVGCRKVM
CCEEEEECCCCHHHHHHHHHCCCCCCCCCCEEEECCCCCCCCCEEEEECCCHHHHHHHHH
EFGYLISKFGGKRKVDALTEKYGVALVFEPTESQNFLIGSSREFVGFHTKINNEVIKCIA
HHHHHHHHCCCCCHHHHHHHHHCEEEEEECCCCCCEEEECCCCEEEEEEHHHHHHHHHHH
NRAIRFYPKMADMMVIRSYAGLRPWTEDHLPIISRVEHIPNYFIAAGHEGDGISLAAVTG
HHHHHHHHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHCCCEEEEECCCCCCEEEEHHHH
KVIEELLNEKETIIPIEPLRLSRFTERVLNG
HHHHHHHCCCCEEECCCCHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: sarcosine; H2O; O2

Specific reaction: sarcosine + H2O + O2 = glycine + formaldehyde + H2O2

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 11481432 [H]