| Definition | Bacillus cereus AH820, complete genome. |
|---|---|
| Accession | NC_011773 |
| Length | 5,302,683 |
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The map label for this gene is pulA [H]
Identifier: 218903853
GI number: 218903853
Start: 2607357
End: 2609915
Strand: Reverse
Name: pulA [H]
Synonym: BCAH820_2737
Alternate gene names: 218903853
Gene position: 2609915-2607357 (Counterclockwise)
Preceding gene: 218903854
Following gene: 218903852
Centisome position: 49.22
GC content: 34.32
Gene sequence:
>2559_bases GTGCAAATTACAAAAAGATTAATTAACAAAGCAGTTTTATTACTTACTATAATTGTTATGTTAGCATCTGTTTTCTCTTT TCAAAGTGTAAAGGCAGTTTCAAATTCGAAAACAACTGAAGTTATCATTCATTACAAAGAGCAGTCTGGAAATACAAAAG ACTGGAACCTTTGGATATGGGGAGAAAATTCAAATGGAAAATCATATGAATTTACTGGTGAAGATGAATTTGGAAAGTAC GCAAAGATTAATATAGATGGTGATTATAACCGTTTAGGATTTATCATTCGTACAATTGAGTGGGAAAAAGATGGTGGCGA TCGTTGGATTGAAAATATAAAGGACGGTCGTGCTGAAGTATGGGTTCTTTCTGGTGATGAAAAAGTATATAACGATAAGC CTTCATCGGATCTCTCAATTCAAAAAGCAACTATTGATAGTTTCCATGAAATTACTGTAACGACGAATGTCCCGTTTAAT ATTAAGGAAAAGAAAATTGAAATGGAAGGCATTAAAATTAAGAGTATTAGTCCTTATGATATAAACAGTGGAGATATTAC GAATAAGGTTAAAATAATTACGGAACAGAAAATTGATTTAAAACAAACATACAAAGTTAAAATAGAAAACTTAGCTGATA CGAATACTGAAATCGGTAAAGTCATTCGTAGTGAGGAGTTTGACTATTTATTTTATTACGGTGGCAACGATTTAGGTAAT ATATATACACCACAACATACAAAGTTCCGTGTATGGGCTCCTACTGCGAGTGAAGCGAAGTTAGTTACATATAAAAAATG GAACGATAAAATAGGTACTGAAATAAATATGCAACAAGGTGAAAAGGGCACCTGGAAAGCAGAACTTAAAGGGAATCAAA AAGGTCTCTATTATACGTATAASGTGAAAATTGGTGATAAGTGGACAGAGGCCGTTGATCCGTATGCACGTGCTGCTTCT GTAAATGGAGATAAAGGTGCGGTTGTTGATTTAGAAGAAACAAATCCGAAAAAATGGAACACTAACAAAAAGCCTAAATT GAAAAATCCGGAAGATGCTATTATTTATGAGTTACATGTACGCGATCTTTCTATTCAGCCCGAAAGTGGTATTAAACAGA AAGGGAAGTATTTAGGTGTAACAGAAAAAGGAACAAAAGGGCCTGAAGGTGTGAAAACGGGACTTGATCATATGAAAGAT CTTGGTGTTACACACGTTCAGCTTTTACCAATATTTGATTATGCTTCAGTAAATGAAGAGAAGGTAAACGAACCACAATA TAACTGGGGATATGACCCAAAAAACTTTAACGTTCCAGAGGGGTCCTATTCTACAAATCCATATGAGCCAACTGTTCGAA TAACTGAATTAAAACAAATGATTCAAACACTACATGATAATAATCTTCGAGTGGTGATGGATGTAGTATATAACCATATG TATAACGCTGCTGAATCTAATTTCCATAAGTTGGTTCCAGGTTATTATTATCGTTACAATGAAGATGGAACATTTGCAAA TGGAACTGGAGTAGGAAATGATACGGCTTCAGAAAGAAAAATGATGAGGAAATTTATGATAGATTCCGTCACATATTGGG CAAAAGAATACAATTTAGATGGATTTCGTTTTGATTTAATGGGTATTCATGATTATGAAACGATGAATGAAATACGTAAA GCTGTTAATCAAATTGACCCTTCTATTATACTGCATGGAGAAGGTTGGGATTTAAATACACCCCTTGCAGCTGAGTTGAA AGCAAATCAAAAAAATGCGGAGAAAATGAAAGGAATTGCCCATTTTAACGATAATATACGTGATGGATTGAAAGGTAGTG TGTTTGAAGAGAAAGAAAATGGATTTGTAAATGGAAAGGAAAACATGGAAGACCGTATTAAAAAAGGTATTACGGCAGGT ATTGACTATGATACAAATTCGTCTACCTATCAAGACCCGGAACAGGTATTAACTTATGTGGAAGCACATGACAATCATAC ATTATGGGATAAACTTGAGTTAACTAATCCAGGTGACAGTGAAGAAGTGCGAAAACAAATGCACAAATTGTCTTCTTCAA TTTTATTAACATCACAAGGAATTCCATTTTTACATGCAGGGCAAGAGTTTATGCGGACGAAATACGGTGATCATAATAGT TACAAATCTCCAGATTCGATTAACCAGATGGATTGGCTGCGCCGTGCAACGTTTAATAATGAAGTAGATTATATGAAGGG TTTAATAGAATTACGCAAAAAGTACCCAGCTTTTCGAATGACATCTGCAGAGCAAATAAAAACACACGTATCATTTATTG ATGCTCCGAAAAATACTGTAACTTATACAATAGAGGGGAATAAACATGAATACTTTACAGTGGCTCACAATGCAAATAGA GAAGCTGTTGAAATAACACTTCCATCTAAAGGGCCTTGGAAAGTACTAGTAGATGGCAAGCAGGCGGGAAGTAAACCACT TTATGTTGTCCATGACAATAAAATTAAAGTTCCTGCATTAAGTTCCATTGTTTTAAAATCAGAAAAACCGATTAAATAA
Upstream 100 bases:
>100_bases CGGGGGGTGAAGCAAAAGAGATTTCTAGATTAATTTAAATTGTGTAATGTAGTACGGGCATAATAAATCGTTCGTACTAC TTAAAGAAAGGGTGTATGTG
Downstream 100 bases:
>100_bases AAGCGAAGAGTGTGGAAATAAATACTGAATTGAGCAAAGTTAATAAGGATTGCAAATGAAAAAAGCGAAGTGAAGATTAT TCTCACTTCGTTTTTAAAGT
Product: putative pullulanase
Products: NA
Alternate protein names: Alpha-dextrin endo-1,6-alpha-glucosidase; Pullulan 6-glucanohydrolase [H]
Number of amino acids: Translated: 852; Mature: 852
Protein sequence:
>852_residues MQITKRLINKAVLLLTIIVMLASVFSFQSVKAVSNSKTTEVIIHYKEQSGNTKDWNLWIWGENSNGKSYEFTGEDEFGKY AKINIDGDYNRLGFIIRTIEWEKDGGDRWIENIKDGRAEVWVLSGDEKVYNDKPSSDLSIQKATIDSFHEITVTTNVPFN IKEKKIEMEGIKIKSISPYDINSGDITNKVKIITEQKIDLKQTYKVKIENLADTNTEIGKVIRSEEFDYLFYYGGNDLGN IYTPQHTKFRVWAPTASEAKLVTYKKWNDKIGTEINMQQGEKGTWKAELKGNQKGLYYTYXVKIGDKWTEAVDPYARAAS VNGDKGAVVDLEETNPKKWNTNKKPKLKNPEDAIIYELHVRDLSIQPESGIKQKGKYLGVTEKGTKGPEGVKTGLDHMKD LGVTHVQLLPIFDYASVNEEKVNEPQYNWGYDPKNFNVPEGSYSTNPYEPTVRITELKQMIQTLHDNNLRVVMDVVYNHM YNAAESNFHKLVPGYYYRYNEDGTFANGTGVGNDTASERKMMRKFMIDSVTYWAKEYNLDGFRFDLMGIHDYETMNEIRK AVNQIDPSIILHGEGWDLNTPLAAELKANQKNAEKMKGIAHFNDNIRDGLKGSVFEEKENGFVNGKENMEDRIKKGITAG IDYDTNSSTYQDPEQVLTYVEAHDNHTLWDKLELTNPGDSEEVRKQMHKLSSSILLTSQGIPFLHAGQEFMRTKYGDHNS YKSPDSINQMDWLRRATFNNEVDYMKGLIELRKKYPAFRMTSAEQIKTHVSFIDAPKNTVTYTIEGNKHEYFTVAHNANR EAVEITLPSKGPWKVLVDGKQAGSKPLYVVHDNKIKVPALSSIVLKSEKPIK
Sequences:
>Translated_852_residues MQITKRLINKAVLLLTIIVMLASVFSFQSVKAVSNSKTTEVIIHYKEQSGNTKDWNLWIWGENSNGKSYEFTGEDEFGKY AKINIDGDYNRLGFIIRTIEWEKDGGDRWIENIKDGRAEVWVLSGDEKVYNDKPSSDLSIQKATIDSFHEITVTTNVPFN IKEKKIEMEGIKIKSISPYDINSGDITNKVKIITEQKIDLKQTYKVKIENLADTNTEIGKVIRSEEFDYLFYYGGNDLGN IYTPQHTKFRVWAPTASEAKLVTYKKWNDKIGTEINMQQGEKGTWKAELKGNQKGLYYTYXVKIGDKWTEAVDPYARAAS VNGDKGAVVDLEETNPKKWNTNKKPKLKNPEDAIIYELHVRDLSIQPESGIKQKGKYLGVTEKGTKGPEGVKTGLDHMKD LGVTHVQLLPIFDYASVNEEKVNEPQYNWGYDPKNFNVPEGSYSTNPYEPTVRITELKQMIQTLHDNNLRVVMDVVYNHM YNAAESNFHKLVPGYYYRYNEDGTFANGTGVGNDTASERKMMRKFMIDSVTYWAKEYNLDGFRFDLMGIHDYETMNEIRK AVNQIDPSIILHGEGWDLNTPLAAELKANQKNAEKMKGIAHFNDNIRDGLKGSVFEEKENGFVNGKENMEDRIKKGITAG IDYDTNSSTYQDPEQVLTYVEAHDNHTLWDKLELTNPGDSEEVRKQMHKLSSSILLTSQGIPFLHAGQEFMRTKYGDHNS YKSPDSINQMDWLRRATFNNEVDYMKGLIELRKKYPAFRMTSAEQIKTHVSFIDAPKNTVTYTIEGNKHEYFTVAHNANR EAVEITLPSKGPWKVLVDGKQAGSKPLYVVHDNKIKVPALSSIVLKSEKPIK >Mature_852_residues MQITKRLINKAVLLLTIIVMLASVFSFQSVKAVSNSKTTEVIIHYKEQSGNTKDWNLWIWGENSNGKSYEFTGEDEFGKY AKINIDGDYNRLGFIIRTIEWEKDGGDRWIENIKDGRAEVWVLSGDEKVYNDKPSSDLSIQKATIDSFHEITVTTNVPFN IKEKKIEMEGIKIKSISPYDINSGDITNKVKIITEQKIDLKQTYKVKIENLADTNTEIGKVIRSEEFDYLFYYGGNDLGN IYTPQHTKFRVWAPTASEAKLVTYKKWNDKIGTEINMQQGEKGTWKAELKGNQKGLYYTYXVKIGDKWTEAVDPYARAAS VNGDKGAVVDLEETNPKKWNTNKKPKLKNPEDAIIYELHVRDLSIQPESGIKQKGKYLGVTEKGTKGPEGVKTGLDHMKD LGVTHVQLLPIFDYASVNEEKVNEPQYNWGYDPKNFNVPEGSYSTNPYEPTVRITELKQMIQTLHDNNLRVVMDVVYNHM YNAAESNFHKLVPGYYYRYNEDGTFANGTGVGNDTASERKMMRKFMIDSVTYWAKEYNLDGFRFDLMGIHDYETMNEIRK AVNQIDPSIILHGEGWDLNTPLAAELKANQKNAEKMKGIAHFNDNIRDGLKGSVFEEKENGFVNGKENMEDRIKKGITAG IDYDTNSSTYQDPEQVLTYVEAHDNHTLWDKLELTNPGDSEEVRKQMHKLSSSILLTSQGIPFLHAGQEFMRTKYGDHNS YKSPDSINQMDWLRRATFNNEVDYMKGLIELRKKYPAFRMTSAEQIKTHVSFIDAPKNTVTYTIEGNKHEYFTVAHNANR EAVEITLPSKGPWKVLVDGKQAGSKPLYVVHDNKIKVPALSSIVLKSEKPIK
Specific function: Hydrolyzes The Alpha-1,6-Glucosidic Linkages In Glycogen Which Has First Been Partially Depolymerized By Phosphorylase. Shows Only Very Little Activity With Native Glycogen. [C]
COG id: COG1523
COG function: function code G; Type II secretory pathway, pullulanase PulA and related glycosidases
Gene ontology:
Cell location: Cytoplasm [C]
Metaboloic importance: Non_Essential [C]
Operon status: Not Known
Operon components: None
Similarity: Belongs to the glycosyl hydrolase 13 family [H]
Homologues:
Organism=Escherichia coli, GI2367229, Length=665, Percent_Identity=22.2556390977444, Blast_Score=118, Evalue=1e-27,
Paralogues:
None
Copy number: NA
Swissprot (AC and ID): NA
Other databases:
- InterPro: IPR006047 - InterPro: IPR004193 - InterPro: IPR017853 - InterPro: IPR013781 - InterPro: IPR013783 - InterPro: IPR014756 - InterPro: IPR005323 - InterPro: IPR011840 [H]
Pfam domain/function: PF00128 Alpha-amylase; PF02922 CBM_48; PF03714 PUD [H]
EC number: =3.2.1.41 [H]
Molecular weight: Translated: 97248; Mature: 97248
Theoretical pI: Translated: 6.82; Mature: 6.82
Prosite motif: NA
Important sites: NA
Signals:
None
Transmembrane regions:
None
Cys/Met content:
0.0 %Cys (Translated Protein) 2.3 %Met (Translated Protein) 2.3 %Cys+Met (Translated Protein) 0.0 %Cys (Mature Protein) 2.3 %Met (Mature Protein) 2.3 %Cys+Met (Mature Protein)
Secondary structure:
>Translated Secondary Structure MQITKRLINKAVLLLTIIVMLASVFSFQSVKAVSNSKTTEVIIHYKEQSGNTKDWNLWIW CCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEEEEEECCCCCCCCEEEEEE GENSNGKSYEFTGEDEFGKYAKINIDGDYNRLGFIIRTIEWEKDGGDRWIENIKDGRAEV EECCCCCEEEECCCCCCCCEEEEEECCCCCCEEEEEEEEEECCCCCHHHHHHHCCCCEEE WVLSGDEKVYNDKPSSDLSIQKATIDSFHEITVTTNVPFNIKEKKIEMEGIKIKSISPYD EEEECCCCCCCCCCCCCCEEEEEECCCEEEEEEEECCCCCCHHCEEEECCEEEECCCCCC INSGDITNKVKIITEQKIDLKQTYKVKIENLADTNTEIGKVIRSEEFDYLFYYGGNDLGN CCCCCCCCEEEEEEECCCCCCEEEEEEEECCCCCCHHHHHHHHCCCCCEEEEECCCCCCC IYTPQHTKFRVWAPTASEAKLVTYKKWNDKIGTEINMQQGEKGTWKAELKGNQKGLYYTY CCCCCCCEEEEECCCCCCCEEEEEEECCCCCCCEEECCCCCCCCEEEEECCCCCCEEEEE XVKIGDKWTEAVDPYARAASVNGDKGAVVDLEETNPKKWNTNKKPKLKNPEDAIIYELHV EEEECCCHHHHCCHHHHHCCCCCCCCCEEEECCCCCCCCCCCCCCCCCCCCCEEEEEEEE RDLSIQPESGIKQKGKYLGVTEKGTKGPEGVKTGLDHMKDLGVTHVQLLPIFDYASVNEE EEEEECCCCCHHHCCCEEEECCCCCCCCHHHHHHHHHHHHCCCCEEEEEEEECCCCCCHH KVNEPQYNWGYDPKNFNVPEGSYSTNPYEPTVRITELKQMIQTLHDNNLRVVMDVVYNHM HCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCEEHHHHHHHHHHHCCCCCEEHHHHHHHHH YNAAESNFHKLVPGYYYRYNEDGTFANGTGVGNDTASERKMMRKFMIDSVTYWAKEYNLD HHHHHCCHHHCCCCEEEEECCCCCEECCCCCCCCCHHHHHHHHHHHHHHHHHHHHHCCCC GFRFDLMGIHDYETMNEIRKAVNQIDPSIILHGEGWDLNTPLAAELKANQKNAEKMKGIA CEEEEEEECCCHHHHHHHHHHHHHCCCEEEEECCCCCCCCCCHHEECCCCCHHHHHHHHH HFNDNIRDGLKGSVFEEKENGFVNGKENMEDRIKKGITAGIDYDTNSSTYQDPEQVLTYV HCCCCHHCCCCCCCEECCCCCCCCCCCCHHHHHHCCCEECCCCCCCCCCCCCHHHHHHHH EAHDNHTLWDKLELTNPGDSEEVRKQMHKLSSSILLTSQGIPFLHAGQEFMRTKYGDHNS HCCCCCEEEEEEEECCCCCHHHHHHHHHHHHCCEEEEECCCCCEECCHHHHHHHCCCCCC YKSPDSINQMDWLRRATFNNEVDYMKGLIELRKKYPAFRMTSAEQIKTHVSFIDAPKNTV CCCCCCCHHHHHHHHHHCCCHHHHHHHHHHHHHHCCCEECCCHHHHHHHHHEEECCCCEE TYTIEGNKHEYFTVAHNANREAVEITLPSKGPWKVLVDGKQAGSKPLYVVHDNKIKVPAL EEEEECCCEEEEEEEECCCCCEEEEECCCCCCEEEEEECCCCCCCCEEEEECCEECCCCH SSIVLKSEKPIK HHEEECCCCCCC >Mature Secondary Structure MQITKRLINKAVLLLTIIVMLASVFSFQSVKAVSNSKTTEVIIHYKEQSGNTKDWNLWIW CCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEEEEEECCCCCCCCEEEEEE GENSNGKSYEFTGEDEFGKYAKINIDGDYNRLGFIIRTIEWEKDGGDRWIENIKDGRAEV EECCCCCEEEECCCCCCCCEEEEEECCCCCCEEEEEEEEEECCCCCHHHHHHHCCCCEEE WVLSGDEKVYNDKPSSDLSIQKATIDSFHEITVTTNVPFNIKEKKIEMEGIKIKSISPYD EEEECCCCCCCCCCCCCCEEEEEECCCEEEEEEEECCCCCCHHCEEEECCEEEECCCCCC INSGDITNKVKIITEQKIDLKQTYKVKIENLADTNTEIGKVIRSEEFDYLFYYGGNDLGN CCCCCCCCEEEEEEECCCCCCEEEEEEEECCCCCCHHHHHHHHCCCCCEEEEECCCCCCC IYTPQHTKFRVWAPTASEAKLVTYKKWNDKIGTEINMQQGEKGTWKAELKGNQKGLYYTY CCCCCCCEEEEECCCCCCCEEEEEEECCCCCCCEEECCCCCCCCEEEEECCCCCCEEEEE XVKIGDKWTEAVDPYARAASVNGDKGAVVDLEETNPKKWNTNKKPKLKNPEDAIIYELHV EEEECCCHHHHCCHHHHHCCCCCCCCCEEEECCCCCCCCCCCCCCCCCCCCCEEEEEEEE RDLSIQPESGIKQKGKYLGVTEKGTKGPEGVKTGLDHMKDLGVTHVQLLPIFDYASVNEE EEEEECCCCCHHHCCCEEEECCCCCCCCHHHHHHHHHHHHCCCCEEEEEEEECCCCCCHH KVNEPQYNWGYDPKNFNVPEGSYSTNPYEPTVRITELKQMIQTLHDNNLRVVMDVVYNHM HCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCEEHHHHHHHHHHHCCCCCEEHHHHHHHHH YNAAESNFHKLVPGYYYRYNEDGTFANGTGVGNDTASERKMMRKFMIDSVTYWAKEYNLD HHHHHCCHHHCCCCEEEEECCCCCEECCCCCCCCCHHHHHHHHHHHHHHHHHHHHHCCCC GFRFDLMGIHDYETMNEIRKAVNQIDPSIILHGEGWDLNTPLAAELKANQKNAEKMKGIA CEEEEEEECCCHHHHHHHHHHHHHCCCEEEEECCCCCCCCCCHHEECCCCCHHHHHHHHH HFNDNIRDGLKGSVFEEKENGFVNGKENMEDRIKKGITAGIDYDTNSSTYQDPEQVLTYV HCCCCHHCCCCCCCEECCCCCCCCCCCCHHHHHHCCCEECCCCCCCCCCCCCHHHHHHHH EAHDNHTLWDKLELTNPGDSEEVRKQMHKLSSSILLTSQGIPFLHAGQEFMRTKYGDHNS HCCCCCEEEEEEEECCCCCHHHHHHHHHHHHCCEEEEECCCCCEECCHHHHHHHCCCCCC YKSPDSINQMDWLRRATFNNEVDYMKGLIELRKKYPAFRMTSAEQIKTHVSFIDAPKNTV CCCCCCCHHHHHHHHHHCCCHHHHHHHHHHHHHHCCCEECCCHHHHHHHHHEEECCCCEE TYTIEGNKHEYFTVAHNANREAVEITLPSKGPWKVLVDGKQAGSKPLYVVHDNKIKVPAL EEEEECCCEEEEEEEECCCCCEEEEECCCCCCEEEEEECCCCCCCCEEEEECCEECCCCH SSIVLKSEKPIK HHEEECCCCCCC
PDB accession: NA
Resolution: NA
Structure class: Alpha Beta
Cofactors: NA
Metal ions: NA
Kcat value (1/min): NA
Specific activity: NA
Km value (mM): NA
Substrates: NA
Specific reaction: NA
General reaction: NA
Inhibitor: NA
Structure determination priority: 9.0
TargetDB status: NA
Availability: NA
References: 9453151; 10360571 [H]