Click here to switch to the map view.

The map label for this gene is pbpB [H]

Identifier: 218903782

GI number: 218903782

Start: 2533884

End: 2536022

Strand: Direct

Name: pbpB [H]

Synonym: BCAH820_2666

Alternate gene names: 218903782

Gene position: 2533884-2536022 (Clockwise)

Preceding gene: 218903781

Following gene: 218903783

Centisome position: 47.78

GC content: 34.64

Gene sequence:

>2139_bases
ATGCATAAACTTCAAACGAATAAAGGCGCGCGCTATTTTATGATTGCTTTTATTGTTTTGTTTCTTATTATGCTTTTGCG
TTTTTTCTATATTCAAGCCGTAGGGGTCGTACATAATGTGAATGTAAAGGATCTTGCAACGGAACAACATAATAAAAATG
GTATTTTGGAAGCAAATCGTGGCACAATATATGATCAAGCTGGAAAAGTCTTAGTTCAAGATTCCACAACGTATCGTGTT
GTTGTGAATTTGAAAGGTAATGGCAAATTGAAAAATAAAGATGAAACCGCAGCGCAATTAGCTGATGCGCTTGAAATTAA
TAAAGAAGATGTGTTAAAGAATTTCCATGAAGGGCGTACGCAAGTTGAAATTGGAAAAGTTGGTCGAAATTTAACGAGAG
AAAAGAAAGAACAAATTAGAAAGTTAAAAATCCCGGGTGTATCTTTTATGCCTGAGAAAGCAAGGGTGTATCCGAATGAA
GATTTTGCATCTTATATACTTGGTTTTGCTAGACCAGACGATAAAGGAAATACAGAAGGGAAATTTGGACTTGAAAAAAG
TTTGGATAAATATTTGCGCTCGACAAACGGGAATATAGAGTATGTGGGAGGGCGAGGAGGTATCCCGCTTACAAATGATA
TAGGAAAAATAGAACCTGCTAAACATGGAAATAACGTATATCTTACGCTTGATAAACAAATTAATAGTTTTTTAGAAGAA
GCGATGAATAAGGCGCAAGATCACTATGATCCTTCTATGTTATTAGGTATCATAGCGGATCCAAAGACGGGGAAAGTGTT
AGCTATGTCTAGTAAACCTAGTTTTAACCCTAATAAAGGGGATATTGAATACTTTTTAAATGATCCAATCGCCAATGCAT
ATGAACCAGGTTCAACGATGAAAGTATTTACGTTAGCTGCAGCAATTAATGAAGGGGTATATAACGGAAAAGAATATTTC
CAATCCGGAAAATATGCAGTTGGTTCATCTGATATAAAAGATCATAATGGTGGATTTGGATGGGGATCTATTACATTTGA
TGAAGGTTTTGAAAGATCATCAAACGTAGCTTTTTCTATTTTAGAAGATCAGTTATTAAAACCAAATAAATTTAAGCAAT
ATATGAACAAATTTGGTTTCAATCAAAAAACTGGAATAGATTTACCTGGTGAAAGTAAAAATACTTTACTATTAAATACA
CAAATTCAACAAGTAACAACTTCTTTCGGACAAGGTTCTACAGTAACTCCAATTCAATTAATCCAAGCCGCTACTGCTAT
AGCAAATGACGGAAAAATGATGCAACCATATATCGTTGACAAAGTTGTAAATCCAACAAACAAACAAGTTATTATGGAAA
ATCAACCGAAAGAAATTGGGAATCCGATAAAAAAAGAGACAGCGGACAAGGTAAGAGACTTAATGGAGCGTGTTATTACG
TCTTCAAAAGGGACTGGTACAATGTATAAAGTGGATGGTTATCCAATAGGAGGTAAGACAGGAACAGCGCAAATTCCGAA
TCCTGAAAATGGACGATATATGGAAGGGAAAGAAAATTATATTTTTTCATTTTTAGGTATGGCTCCGATTGATGATCCGG
AGTTAATTGTATACTTGGCAATTAAACAACCAAAGTTAAAGGGAGACGAGTATGGAGCTCAGCCGCTTGCTGAAATATTT
AAACCAGTTGTGAAAAATAGCCTTGAATATTTAAAGGTGAAACCATACACAGAAAAACAAATGGCCGATGCAACGGAAAA
ATCCCAATTAAAAGTGCAAAACTATGTGAATCAATCCATGGATACGGTAGAGAATAATGTAAAAAAAGAGAGGCTCCAAC
CAATAACTTTAGGAGAAGGAAAAATAATAAAACAATATCCGCAGTCTGGCGAAGTAATGAGTGAAGGAGATCGAATATTT
TTATTAGGAAAAAACGCTACGATGCCCAATTTACAAGGTTGGTCAATGCGTGATGTGATGTATTTTTCTAAACTATTAAA
ATTAGATTTAAAAACTTCAGGTACGGGATATGTAACAAGTCAAAGTATCAAAAAAGGACAAGCTATACAAGAAGGCGATG
TATTGGAACTAGAATTAGAGCCACCGTTACAACCGTTAACAGAGGCAAATACAAATTAA

Upstream 100 bases:

>100_bases
ATTCATACGAAAACTTCGAAACATATAGTATTGAAAGTAAAAATTACATATTTTAAAGCGCATCTTTGCATGTATCAATT
AAAAAAGAAAGGAGCGAAAT

Downstream 100 bases:

>100_bases
TTAGGATATGTGAAGGGTCTATATATAATAGACTCTTTTTTTAATGGTTTAAGTAATTTCACGCACCGTAATTTTTAGAA
AAAATAATAAATAAGATTTT

Product: penicillin-binding protein

Products: NA

Alternate protein names: PBP-2B; Penicillin-binding protein B [H]

Number of amino acids: Translated: 712; Mature: 712

Protein sequence:

>712_residues
MHKLQTNKGARYFMIAFIVLFLIMLLRFFYIQAVGVVHNVNVKDLATEQHNKNGILEANRGTIYDQAGKVLVQDSTTYRV
VVNLKGNGKLKNKDETAAQLADALEINKEDVLKNFHEGRTQVEIGKVGRNLTREKKEQIRKLKIPGVSFMPEKARVYPNE
DFASYILGFARPDDKGNTEGKFGLEKSLDKYLRSTNGNIEYVGGRGGIPLTNDIGKIEPAKHGNNVYLTLDKQINSFLEE
AMNKAQDHYDPSMLLGIIADPKTGKVLAMSSKPSFNPNKGDIEYFLNDPIANAYEPGSTMKVFTLAAAINEGVYNGKEYF
QSGKYAVGSSDIKDHNGGFGWGSITFDEGFERSSNVAFSILEDQLLKPNKFKQYMNKFGFNQKTGIDLPGESKNTLLLNT
QIQQVTTSFGQGSTVTPIQLIQAATAIANDGKMMQPYIVDKVVNPTNKQVIMENQPKEIGNPIKKETADKVRDLMERVIT
SSKGTGTMYKVDGYPIGGKTGTAQIPNPENGRYMEGKENYIFSFLGMAPIDDPELIVYLAIKQPKLKGDEYGAQPLAEIF
KPVVKNSLEYLKVKPYTEKQMADATEKSQLKVQNYVNQSMDTVENNVKKERLQPITLGEGKIIKQYPQSGEVMSEGDRIF
LLGKNATMPNLQGWSMRDVMYFSKLLKLDLKTSGTGYVTSQSIKKGQAIQEGDVLELELEPPLQPLTEANTN

Sequences:

>Translated_712_residues
MHKLQTNKGARYFMIAFIVLFLIMLLRFFYIQAVGVVHNVNVKDLATEQHNKNGILEANRGTIYDQAGKVLVQDSTTYRV
VVNLKGNGKLKNKDETAAQLADALEINKEDVLKNFHEGRTQVEIGKVGRNLTREKKEQIRKLKIPGVSFMPEKARVYPNE
DFASYILGFARPDDKGNTEGKFGLEKSLDKYLRSTNGNIEYVGGRGGIPLTNDIGKIEPAKHGNNVYLTLDKQINSFLEE
AMNKAQDHYDPSMLLGIIADPKTGKVLAMSSKPSFNPNKGDIEYFLNDPIANAYEPGSTMKVFTLAAAINEGVYNGKEYF
QSGKYAVGSSDIKDHNGGFGWGSITFDEGFERSSNVAFSILEDQLLKPNKFKQYMNKFGFNQKTGIDLPGESKNTLLLNT
QIQQVTTSFGQGSTVTPIQLIQAATAIANDGKMMQPYIVDKVVNPTNKQVIMENQPKEIGNPIKKETADKVRDLMERVIT
SSKGTGTMYKVDGYPIGGKTGTAQIPNPENGRYMEGKENYIFSFLGMAPIDDPELIVYLAIKQPKLKGDEYGAQPLAEIF
KPVVKNSLEYLKVKPYTEKQMADATEKSQLKVQNYVNQSMDTVENNVKKERLQPITLGEGKIIKQYPQSGEVMSEGDRIF
LLGKNATMPNLQGWSMRDVMYFSKLLKLDLKTSGTGYVTSQSIKKGQAIQEGDVLELELEPPLQPLTEANTN
>Mature_712_residues
MHKLQTNKGARYFMIAFIVLFLIMLLRFFYIQAVGVVHNVNVKDLATEQHNKNGILEANRGTIYDQAGKVLVQDSTTYRV
VVNLKGNGKLKNKDETAAQLADALEINKEDVLKNFHEGRTQVEIGKVGRNLTREKKEQIRKLKIPGVSFMPEKARVYPNE
DFASYILGFARPDDKGNTEGKFGLEKSLDKYLRSTNGNIEYVGGRGGIPLTNDIGKIEPAKHGNNVYLTLDKQINSFLEE
AMNKAQDHYDPSMLLGIIADPKTGKVLAMSSKPSFNPNKGDIEYFLNDPIANAYEPGSTMKVFTLAAAINEGVYNGKEYF
QSGKYAVGSSDIKDHNGGFGWGSITFDEGFERSSNVAFSILEDQLLKPNKFKQYMNKFGFNQKTGIDLPGESKNTLLLNT
QIQQVTTSFGQGSTVTPIQLIQAATAIANDGKMMQPYIVDKVVNPTNKQVIMENQPKEIGNPIKKETADKVRDLMERVIT
SSKGTGTMYKVDGYPIGGKTGTAQIPNPENGRYMEGKENYIFSFLGMAPIDDPELIVYLAIKQPKLKGDEYGAQPLAEIF
KPVVKNSLEYLKVKPYTEKQMADATEKSQLKVQNYVNQSMDTVENNVKKERLQPITLGEGKIIKQYPQSGEVMSEGDRIF
LLGKNATMPNLQGWSMRDVMYFSKLLKLDLKTSGTGYVTSQSIKKGQAIQEGDVLELELEPPLQPLTEANTN

Specific function: Penicillin-binding proteins (PBPs) function in the late steps of murein biosynthesis. PBP-2B is required for vegetative cell division and sporulation septation. Beta-lactamase inactivates the PBPs by acylating an essential serine residue in the active sit

COG id: COG0768

COG function: function code M; Cell division protein FtsI/penicillin-binding protein 2

Gene ontology:

Cell location: Cell membrane; Single-pass membrane protein [H]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 2 PASTA domains [H]

Homologues:

Organism=Escherichia coli, GI1786272, Length=577, Percent_Identity=26.8630849220104, Blast_Score=155, Evalue=7e-39,
Organism=Escherichia coli, GI1786854, Length=562, Percent_Identity=25.0889679715303, Blast_Score=120, Evalue=4e-28,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR012338
- InterPro:   IPR005543
- InterPro:   IPR005311
- InterPro:   IPR001460 [H]

Pfam domain/function: PF03793 PASTA; PF03717 PBP_dimer; PF00905 Transpeptidase [H]

EC number: NA

Molecular weight: Translated: 79262; Mature: 79262

Theoretical pI: Translated: 9.13; Mature: 9.13

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
3.1 %Met     (Translated Protein)
3.1 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
3.1 %Met     (Mature Protein)
3.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MHKLQTNKGARYFMIAFIVLFLIMLLRFFYIQAVGVVHNVNVKDLATEQHNKNGILEANR
CCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHCCCCCCEECCC
GTIYDQAGKVLVQDSTTYRVVVNLKGNGKLKNKDETAAQLADALEINKEDVLKNFHEGRT
CEEECCCCCEEEECCCEEEEEEEECCCCCCCCCHHHHHHHHHHHHCCHHHHHHHHHCCCC
QVEIGKVGRNLTREKKEQIRKLKIPGVSFMPEKARVYPNEDFASYILGFARPDDKGNTEG
EEEHHHHCCHHHHHHHHHHHEECCCCCCCCCCCCCCCCCHHHHHHHHHCCCCCCCCCCCC
KFGLEKSLDKYLRSTNGNIEYVGGRGGIPLTNDIGKIEPAKHGNNVYLTLDKQINSFLEE
CCCHHHHHHHHHHHCCCCEEEECCCCCCCCCCCCCCCCCCCCCCEEEEEECHHHHHHHHH
AMNKAQDHYDPSMLLGIIADPKTGKVLAMSSKPSFNPNKGDIEYFLNDPIANAYEPGSTM
HHHHHHHCCCCCCEEEEEECCCCCCEEEECCCCCCCCCCCCEEEEECCCCCCCCCCCCCE
KVFTLAAAINEGVYNGKEYFQSGKYAVGSSDIKDHNGGFGWGSITFDEGFERSSNVAFSI
EHHHHHHHHHCCCCCCHHHHHCCCEEECCCCCCCCCCCCCCCEEECCCCCCCCCCCEEEE
LEDQLLKPNKFKQYMNKFGFNQKTGIDLPGESKNTLLLNTQIQQVTTSFGQGSTVTPIQL
HHHHCCCCHHHHHHHHHHCCCCCCCCCCCCCCCCEEEEECHHHHHHHHCCCCCCCCHHHH
IQAATAIANDGKMMQPYIVDKVVNPTNKQVIMENQPKEIGNPIKKETADKVRDLMERVIT
HHHHHHHCCCCCEECCHHHHHHCCCCCCEEEECCCCHHHCCHHHHHHHHHHHHHHHHHHH
SSKGTGTMYKVDGYPIGGKTGTAQIPNPENGRYMEGKENYIFSFLGMAPIDDPELIVYLA
CCCCCCEEEEECCEECCCCCCCCCCCCCCCCCEECCCCCEEEEEECCCCCCCCCEEEEEE
IKQPKLKGDEYGAQPLAEIFKPVVKNSLEYLKVKPYTEKQMADATEKSQLKVQNYVNQSM
EECCCCCCCCCCCCHHHHHHHHHHHCCHHEEEECCCCHHHHHCCHHHHHHHHHHHHHHHH
DTVENNVKKERLQPITLGEGKIIKQYPQSGEVMSEGDRIFLLGKNATMPNLQGWSMRDVM
HHHHHHHHHHHCCCEECCCCCHHHHCCCCCCEECCCCEEEEEECCCCCCCCCCCCHHHHH
YFSKLLKLDLKTSGTGYVTSQSIKKGQAIQEGDVLELELEPPLQPLTEANTN
HHHHHHHHCCCCCCCCEEEHHHHHCCCCCCCCCEEEEECCCCCCCCCCCCCC
>Mature Secondary Structure
MHKLQTNKGARYFMIAFIVLFLIMLLRFFYIQAVGVVHNVNVKDLATEQHNKNGILEANR
CCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHCCCCCCEECCC
GTIYDQAGKVLVQDSTTYRVVVNLKGNGKLKNKDETAAQLADALEINKEDVLKNFHEGRT
CEEECCCCCEEEECCCEEEEEEEECCCCCCCCCHHHHHHHHHHHHCCHHHHHHHHHCCCC
QVEIGKVGRNLTREKKEQIRKLKIPGVSFMPEKARVYPNEDFASYILGFARPDDKGNTEG
EEEHHHHCCHHHHHHHHHHHEECCCCCCCCCCCCCCCCCHHHHHHHHHCCCCCCCCCCCC
KFGLEKSLDKYLRSTNGNIEYVGGRGGIPLTNDIGKIEPAKHGNNVYLTLDKQINSFLEE
CCCHHHHHHHHHHHCCCCEEEECCCCCCCCCCCCCCCCCCCCCCEEEEEECHHHHHHHHH
AMNKAQDHYDPSMLLGIIADPKTGKVLAMSSKPSFNPNKGDIEYFLNDPIANAYEPGSTM
HHHHHHHCCCCCCEEEEEECCCCCCEEEECCCCCCCCCCCCEEEEECCCCCCCCCCCCCE
KVFTLAAAINEGVYNGKEYFQSGKYAVGSSDIKDHNGGFGWGSITFDEGFERSSNVAFSI
EHHHHHHHHHCCCCCCHHHHHCCCEEECCCCCCCCCCCCCCCEEECCCCCCCCCCCEEEE
LEDQLLKPNKFKQYMNKFGFNQKTGIDLPGESKNTLLLNTQIQQVTTSFGQGSTVTPIQL
HHHHCCCCHHHHHHHHHHCCCCCCCCCCCCCCCCEEEEECHHHHHHHHCCCCCCCCHHHH
IQAATAIANDGKMMQPYIVDKVVNPTNKQVIMENQPKEIGNPIKKETADKVRDLMERVIT
HHHHHHHCCCCCEECCHHHHHHCCCCCCEEEECCCCHHHCCHHHHHHHHHHHHHHHHHHH
SSKGTGTMYKVDGYPIGGKTGTAQIPNPENGRYMEGKENYIFSFLGMAPIDDPELIVYLA
CCCCCCEEEEECCEECCCCCCCCCCCCCCCCCEECCCCCEEEEEECCCCCCCCCEEEEEE
IKQPKLKGDEYGAQPLAEIFKPVVKNSLEYLKVKPYTEKQMADATEKSQLKVQNYVNQSM
EECCCCCCCCCCCCHHHHHHHHHHHCCHHEEEECCCCHHHHHCCHHHHHHHHHHHHHHHH
DTVENNVKKERLQPITLGEGKIIKQYPQSGEVMSEGDRIFLLGKNATMPNLQGWSMRDVM
HHHHHHHHHHHCCCEECCCCCHHHHCCCCCCEECCCCEEEEEECCCCCCCCCCCCHHHHH
YFSKLLKLDLKTSGTGYVTSQSIKKGQAIQEGDVLELELEPPLQPLTEANTN
HHHHHHHHCCCCCCCCEEEHHHHHCCCCCCCCCEEEEECCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 8244929; 9384377; 8289242 [H]