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The map label for this gene is murF [H]

Identifier: 218903320

GI number: 218903320

Start: 2094910

End: 2095650

Strand: Direct

Name: murF [H]

Synonym: BCAH820_2204

Alternate gene names: 218903320

Gene position: 2094910-2095650 (Clockwise)

Preceding gene: 218903318

Following gene: 218903321

Centisome position: 39.51

GC content: 33.65

Gene sequence:

>741_bases
TTGATTCAAATGAATTTGAAAAAACTTGAAGAGGTAATAAATGGAGAAGGATTACACGAATCGTTTCATCATTTAGAAAT
ACATGGTGTCTGCATAGACTCCAAAAATATGAAAGAAGGAAATGTATTCGTTCCAATCATTAGGGTCAAAGATGGACATG
ATTATGTGAAAGAGGCAATGGATAATGGAGCTGTCGCTTCCTTATGGAAAAAGTCTTACGGTACTCCGCCAAAAGGGGTT
CCAATTATATTTGTAGATGACACTTTGTTTGCTTTGCAACAATTGGCACAATTTTATCGAAGAGAGATAAATGTAAAAGT
AATCGGAATTACCGGTAGTAATGGAAAAACTACGGTAAAAGATATAATAGAAGGTATTTTAAGGTCAACTTATAGAGTGC
ATAAAACAAAAGGTAATTTTAATAGTCAAATCGGTTTRCCGTTAACAATTTTAGAGATGAAACGAGATACAGAGTTTTTA
ATACTTGAAATGGGGATGAGTGAAAAAGGATGTCGTATTATTGAAAGGTTCACGTGGAATAGCTTTAGAGGAAATTGTCC
AAAAGTGGATGTGAAAAATCAAAATTTATTTAAAAATGCTTGCATTTTACAAAGTGAAGAGATAGAATACTCTGTAAATA
TAAAAAGTGATGATCAGGAAAAGTACATGATGCAAAGATCTACAGAGAGCAATCGGTTGCTGGAAAGATTGCGATACCGC
ATGATGGAAAGACACCTGTGA

Upstream 100 bases:

>100_bases
AACTAATTGCTTTTTTCATATAAACACCTCACTATAAAATTTTTATTAATATGCGTAAATAAAACTGAAAATATGTAATA
AAATGGAAATAGGTGATAAT

Downstream 100 bases:

>100_bases
GTGTTGCTTTGAACGTGATAATTAGTAAAAGCAAACGGTACCTACCGTTATCAGGACTAAGATGGTCATTATGACAATTT
GGGTGGTACCGCGGAAAAAT

Product: UDP-N-acetylmuramoylalanyl-D-glutamyl-2, 6-diaminopimelate--D-alanyl-D-alanyl ligase

Products: NA

Alternate protein names: D-alanyl-D-alanine-adding enzyme; UDP-MurNAc-pentapeptide synthetase [H]

Number of amino acids: Translated: 246; Mature: 246

Protein sequence:

>246_residues
MIQMNLKKLEEVINGEGLHESFHHLEIHGVCIDSKNMKEGNVFVPIIRVKDGHDYVKEAMDNGAVASLWKKSYGTPPKGV
PIIFVDDTLFALQQLAQFYRREINVKVIGITGSNGKTTVKDIIEGILRSTYRVHKTKGNFNSQIGLPLTILEMKRDTEFL
ILEMGMSEKGCRIIERFTWNSFRGNCPKVDVKNQNLFKNACILQSEEIEYSVNIKSDDQEKYMMQRSTESNRLLERLRYR
MMERHL

Sequences:

>Translated_246_residues
MIQMNLKKLEEVINGEGLHESFHHLEIHGVCIDSKNMKEGNVFVPIIRVKDGHDYVKEAMDNGAVASLWKKSYGTPPKGV
PIIFVDDTLFALQQLAQFYRREINVKVIGITGSNGKTTVKDIIEGILRSTYRVHKTKGNFNSQIGLPLTILEMKRDTEFL
ILEMGMSEKGCRIIERFTWNSFRGNCPKVDVKNQNLFKNACILQSEEIEYSVNIKSDDQEKYMMQRSTESNRLLERLRYR
MMERHL
>Mature_246_residues
MIQMNLKKLEEVINGEGLHESFHHLEIHGVCIDSKNMKEGNVFVPIIRVKDGHDYVKEAMDNGAVASLWKKSYGTPPKGV
PIIFVDDTLFALQQLAQFYRREINVKVIGITGSNGKTTVKDIIEGILRSTYRVHKTKGNFNSQIGLPLTILEMKRDTEFL
ILEMGMSEKGCRIIERFTWNSFRGNCPKVDVKNQNLFKNACILQSEEIEYSVNIKSDDQEKYMMQRSTESNRLLERLRYR
MMERHL

Specific function: Involved in cell wall formation. Catalyzes the final step in the synthesis of UDP-N-acetylmuramoyl-pentapeptide, the precursor of murein [H]

COG id: COG0770

COG function: function code M; UDP-N-acetylmuramyl pentapeptide synthase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the MurCDEF family [H]

Homologues:

Organism=Escherichia coli, GI1786274, Length=171, Percent_Identity=30.4093567251462, Blast_Score=94, Evalue=6e-21,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004101
- InterPro:   IPR013221
- InterPro:   IPR000713
- InterPro:   IPR005863 [H]

Pfam domain/function: PF01225 Mur_ligase; PF02875 Mur_ligase_C; PF08245 Mur_ligase_M [H]

EC number: =6.3.2.10 [H]

Molecular weight: Translated: 28460; Mature: 28460

Theoretical pI: Translated: 8.86; Mature: 8.86

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.6 %Cys     (Translated Protein)
4.5 %Met     (Translated Protein)
6.1 %Cys+Met (Translated Protein)
1.6 %Cys     (Mature Protein)
4.5 %Met     (Mature Protein)
6.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MIQMNLKKLEEVINGEGLHESFHHLEIHGVCIDSKNMKEGNVFVPIIRVKDGHDYVKEAM
CCCCCHHHHHHHHCCCCCCCHHHEEEEEEEEECCCCCCCCCEEEEEEEEECCHHHHHHHH
DNGAVASLWKKSYGTPPKGVPIIFVDDTLFALQQLAQFYRREINVKVIGITGSNGKTTVK
CCCHHHHHHHHHCCCCCCCEEEEEECCHHHHHHHHHHHHHHHCCEEEEEEECCCCCCHHH
DIIEGILRSTYRVHKTKGNFNSQIGLPLTILEMKRDTEFLILEMGMSEKGCRIIERFTWN
HHHHHHHHHHHEEEECCCCCCCCCCCCEEEEEECCCCEEEEEEECCCCCCCHHHHHHCCC
SFRGNCPKVDVKNQNLFKNACILQSEEIEYSVNIKSDDQEKYMMQRSTESNRLLERLRYR
CCCCCCCEEECCCCCHHHHHHEEECCCEEEEEEECCCCHHHHHHHHCCCHHHHHHHHHHH
MMERHL
HHHHCC
>Mature Secondary Structure
MIQMNLKKLEEVINGEGLHESFHHLEIHGVCIDSKNMKEGNVFVPIIRVKDGHDYVKEAM
CCCCCHHHHHHHHCCCCCCCHHHEEEEEEEEECCCCCCCCCEEEEEEEEECCHHHHHHHH
DNGAVASLWKKSYGTPPKGVPIIFVDDTLFALQQLAQFYRREINVKVIGITGSNGKTTVK
CCCHHHHHHHHHCCCCCCCEEEEEECCHHHHHHHHHHHHHHHCCEEEEEEECCCCCCHHH
DIIEGILRSTYRVHKTKGNFNSQIGLPLTILEMKRDTEFLILEMGMSEKGCRIIERFTWN
HHHHHHHHHHHEEEECCCCCCCCCCCCEEEEEECCCCEEEEEEECCCCCCCHHHHHHCCC
SFRGNCPKVDVKNQNLFKNACILQSEEIEYSVNIKSDDQEKYMMQRSTESNRLLERLRYR
CCCCCCCEEECCCCCHHHHHHEEECCCEEEEEEECCCCHHHHHHHHCCCHHHHHHHHHHH
MMERHL
HHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9384377 [H]