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The map label for this gene is yofA [H]

Identifier: 218903089

GI number: 218903089

Start: 1879543

End: 1880331

Strand: Reverse

Name: yofA [H]

Synonym: BCAH820_1972

Alternate gene names: 218903089

Gene position: 1880331-1879543 (Counterclockwise)

Preceding gene: 218903101

Following gene: 218903079

Centisome position: 35.46

GC content: 30.04

Gene sequence:

>789_bases
ATGGAAATAAATGATCTTATTATATTCAAAACTGTAGCTAGCGAAGGTTCTATTAGTAAAGCTGCGAAAGAGTTAGGATA
TGTTCAACCAAACGTAACTGAGCGAATCAAAAAATTAGAACTAGAATTAGAAACACCTTTACTACATAGAGATAACAAAG
GCGTTTCGTTGTTACCTTCTGGTGACATTTTACTAGACTACACAAATCAAATATTAACGCTAGTAGAAGAAGCGAAAGAT
AAAATTAAAATGTGTGGTTCTTCTTATATAATCGCGACGTCACAATCGATTTTGACTAATTATGTAAGTAAGCGCATTAA
AGAAAATTTCAGGAATTATCAAATATATGTAGAAAATAGTAGTCATTTACAAAAATTACTGCAGCAACAAAAAGTTGATA
TGGTCATTACTTATGAGGATTACCCGGACACAGCGTTTAAAAAAGTATTTTCCTCTTCAATTTCTATCGGTTTATTAAAA
GTTAAAGAAAAGTGTACCGTTGATTATTCAAAGGAACTCTTCTTCGTTAGCAATGACAAAAAGTGCCCTTTTAGAAATAA
GACTATTCAATTTCTAAAAGAAAATAATTTATCTCAGCGTCAACTTCAACAACTAGATTCTTATTCGCTTATGGAAGAGT
ATATTCAAGACGGAAAAGGCATAGCCTTTTTACCGATTAATAATGATAAATTGGTACCAATTGAAGATGTTCAAATTGAG
AAATTAACTATCCATTTTTTCACAAATCAAAACTCTTTAAAACAAATCCCTGATGAATTATTTAATTAA

Upstream 100 bases:

>100_bases
CCTATATACATAATGATAAAGCGATGACTGACTAACGTAAAATAGGTATAACTTATGCTTGGTATCATTTATAATGATAT
CGTATAAGGAGCTGTGATAA

Downstream 100 bases:

>100_bases
TAAAAAATCCCTCCTTTACGAATCTAAAAGAGGGATTTTCATATTTTATACAATCGATTTTTCACACGTTTTGCTCCACT
TCTGCACTTTTCTCCTTCTT

Product: transcriptional regulator, LysR family

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 262; Mature: 262

Protein sequence:

>262_residues
MEINDLIIFKTVASEGSISKAAKELGYVQPNVTERIKKLELELETPLLHRDNKGVSLLPSGDILLDYTNQILTLVEEAKD
KIKMCGSSYIIATSQSILTNYVSKRIKENFRNYQIYVENSSHLQKLLQQQKVDMVITYEDYPDTAFKKVFSSSISIGLLK
VKEKCTVDYSKELFFVSNDKKCPFRNKTIQFLKENNLSQRQLQQLDSYSLMEEYIQDGKGIAFLPINNDKLVPIEDVQIE
KLTIHFFTNQNSLKQIPDELFN

Sequences:

>Translated_262_residues
MEINDLIIFKTVASEGSISKAAKELGYVQPNVTERIKKLELELETPLLHRDNKGVSLLPSGDILLDYTNQILTLVEEAKD
KIKMCGSSYIIATSQSILTNYVSKRIKENFRNYQIYVENSSHLQKLLQQQKVDMVITYEDYPDTAFKKVFSSSISIGLLK
VKEKCTVDYSKELFFVSNDKKCPFRNKTIQFLKENNLSQRQLQQLDSYSLMEEYIQDGKGIAFLPINNDKLVPIEDVQIE
KLTIHFFTNQNSLKQIPDELFN
>Mature_262_residues
MEINDLIIFKTVASEGSISKAAKELGYVQPNVTERIKKLELELETPLLHRDNKGVSLLPSGDILLDYTNQILTLVEEAKD
KIKMCGSSYIIATSQSILTNYVSKRIKENFRNYQIYVENSSHLQKLLQQQKVDMVITYEDYPDTAFKKVFSSSISIGLLK
VKEKCTVDYSKELFFVSNDKKCPFRNKTIQFLKENNLSQRQLQQLDSYSLMEEYIQDGKGIAFLPINNDKLVPIEDVQIE
KLTIHFFTNQNSLKQIPDELFN

Specific function: Regulates expression of the cell division protein ftsW, and is essential for cell viability during stationary phase [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cytoplasmic [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 HTH lysR-type DNA-binding domain [H]

Homologues:

Organism=Escherichia coli, GI1787806, Length=240, Percent_Identity=25, Blast_Score=69, Evalue=4e-13,

Paralogues:

None

Copy number: 10-20 Molecules/Cell [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000847
- InterPro:   IPR005119
- InterPro:   IPR011991 [H]

Pfam domain/function: PF00126 HTH_1; PF03466 LysR_substrate [H]

EC number: NA

Molecular weight: Translated: 30208; Mature: 30208

Theoretical pI: Translated: 6.30; Mature: 6.30

Prosite motif: PS50931 HTH_LYSR

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
1.5 %Met     (Translated Protein)
2.7 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
1.5 %Met     (Mature Protein)
2.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MEINDLIIFKTVASEGSISKAAKELGYVQPNVTERIKKLELELETPLLHRDNKGVSLLPS
CCCCCEEEEEEECCCCCHHHHHHHHCCCCCCHHHHHHHHHHEECCCEEECCCCCEEEECC
GDILLDYTNQILTLVEEAKDKIKMCGSSYIIATSQSILTNYVSKRIKENFRNYQIYVENS
CCEEEEHHHHHHHHHHHHHHHHHHCCCCEEEEEHHHHHHHHHHHHHHHCCCCEEEEEECC
SHLQKLLQQQKVDMVITYEDYPDTAFKKVFSSSISIGLLKVKEKCTVDYSKELFFVSNDK
HHHHHHHHHCCCCEEEEECCCCCHHHHHHHHCCCCEEEEEEHHHHCCCCCCEEEEEECCC
KCPFRNKTIQFLKENNLSQRQLQQLDSYSLMEEYIQDGKGIAFLPINNDKLVPIEDVQIE
CCCCCHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHCCCCEEEEEECCCCEEEECCCEEE
KLTIHFFTNQNSLKQIPDELFN
EEEEEEEECCCHHHHHHHHHCC
>Mature Secondary Structure
MEINDLIIFKTVASEGSISKAAKELGYVQPNVTERIKKLELELETPLLHRDNKGVSLLPS
CCCCCEEEEEEECCCCCHHHHHHHHCCCCCCHHHHHHHHHHEECCCEEECCCCCEEEECC
GDILLDYTNQILTLVEEAKDKIKMCGSSYIIATSQSILTNYVSKRIKENFRNYQIYVENS
CCEEEEHHHHHHHHHHHHHHHHHHCCCCEEEEEHHHHHHHHHHHHHHHCCCCEEEEEECC
SHLQKLLQQQKVDMVITYEDYPDTAFKKVFSSSISIGLLKVKEKCTVDYSKELFFVSNDK
HHHHHHHHHCCCCEEEEECCCCCHHHHHHHHCCCCEEEEEEHHHHCCCCCCEEEEEECCC
KCPFRNKTIQFLKENNLSQRQLQQLDSYSLMEEYIQDGKGIAFLPINNDKLVPIEDVQIE
CCCCCHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHCCCCEEEEEECCCCEEEECCCEEE
KLTIHFFTNQNSLKQIPDELFN
EEEEEEEECCCHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9384377 [H]