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The map label for this gene is gatA2 [H]

Identifier: 218903074

GI number: 218903074

Start: 1863199

End: 1864809

Strand: Direct

Name: gatA2 [H]

Synonym: BCAH820_1957

Alternate gene names: 218903074

Gene position: 1863199-1864809 (Clockwise)

Preceding gene: 218903073

Following gene: 218903075

Centisome position: 35.14

GC content: 35.57

Gene sequence:

>1611_bases
ATGAAGAAATGGGTGAAGGTAACGCTGTCTATCGCTGGAGGCATAGTCTTAGTAGCATGTGCCAGCGGATATTACGTGTA
TAAAAATTATTTTCCAAAGGAACCTGAGCGTATTGTATATGATAAAGAAAGAGTGCTACAACCGATACATAATCAGCTTA
AAGGAATCAATATAGAAAATGTAAAGATAAAAGAAAAAGAAGTTGTAAATGCGACTGTAGATGAGCTTCAAAAAATGATT
GATGATGGAAAATTATCATATGAAGAATTAACGAGTATTTATCTCTTTAGAATACAAGAACATGATCAAAATGGAATAAC
ATTAAATTCTGTTACAGAGATAAATCCTAATGCGATGGAAGAAGCAAGAAAGTTAGATCAAGAGCGAAGTAGAAACAAAA
AGTCGAATTTATATGGTATTCCTGTCGTTGTAAAAGATAATGTACAAACGGCAAAAGTGATGCCGACTAGTGCAGGAACT
TATGTATTAAAAGATTGGATTGCAGATCAAGACGCAACAATTGTGAAGCAGTTGAAAGAAGAAGGGGCATTTGTTTTAGG
AAAAGCGAATATGTCTGAGTGGGCGAATTATTTATCTTTTACAATGCCTAGCGGATATAGCGGGAAAAAAGGACAAAATT
TGAATCCATACGGTCCGATTATGTTTGATACATCGGGATCAAGTTCAGGATCTGCTACAGTAGTTACAGCAGATTTTGCA
CCGCTTGCAGTCGGAACAGAAACGACAGGATCGATTGTAGCACCCGCAGCGCAGCAATCAGTAGTTGGGTTACGTCCATC
TTTAGGTATGGTAAGTAGAACAGGAATTATTCCGTTAGCTGAAACTCTTGATACAGCAGGACCTATGGCAAGAACGGTAA
AAGATGCAGCAACGTTATTTAATGCAATGATAGGTTATGACGAAAAAGATGTTATGACAGAAAAAGTGAAAGATAAAGAA
AGAATTGATTATACAAAGGATTTATCAATAGATGGATTAAAAGGGAAAAAGATAGGGCTTCTTTTTTCTGTAGACCAACA
AGATGAAAATAGAAAAGCAGTAGCAGAAAAGATTAGAAAAGACCTTCAAGATGCAGGTGCGATATTAACTGATTATATTC
AGTTAAACAATGGGGGTGTAGATAATTTACAAACATTAGAATATGAATTCAAGCATAATGTAAACGACTATTTTTCACAG
CAAAAAAATGTGCCGGTAAAATCGTTAGAAGAGATTATAGCGTTTAATAAAAAGGATAGTAAAAAACGAATAAAATACGG
ACAAACATTAATTGAGGCGTCTGAAAAATCTGCTATAACGAAAGATGAGTTTAAAAAAGTAGTGCAAACGAGTCAAGAAA
ATGCAAAAAAAGAGCTAGATAGATATTTAGTAGAAAAGGGTTTAGACGCTTTAGTTATGATTAACAACGAGGAAGTTCTT
CTATCCGCTGTAGCTGGCTATCCAGAATTAGCGGTGCCAGCAGGATATGATAACAATGGAGAGCCAGTAGGTGCAGTATT
TGTCGGGAAGCAATTCGGTGAGAAGGAACTGTTCAATATTGGATATGCGTATGAACAGCAGTCTAAAAATAGAAAACCAC
CTAAACTGTAA

Upstream 100 bases:

>100_bases
TCTTTCATTTTTTCATTTAGCATTTTGATTGGAGAGAGTTAAGTAAAAGGTAACTCTCTTTTTCGTATAAAGAAGCATCT
TATAGAGTGGAGGAAGCAAC

Downstream 100 bases:

>100_bases
AAGGTTAAACGAAAAAAAGAGCTCAACTGAAGTAAGTAGGTGGGCTAATATAAATAGAATCTAACAATATAAAAAGAAAA
AAAGGATTCTTTCTAATTAG

Product: amidase

Products: ADP; phosphate; Gln-tRNA(Gln); L-glutamate

Alternate protein names: Glu-ADT subunit A 2 [H]

Number of amino acids: Translated: 536; Mature: 536

Protein sequence:

>536_residues
MKKWVKVTLSIAGGIVLVACASGYYVYKNYFPKEPERIVYDKERVLQPIHNQLKGINIENVKIKEKEVVNATVDELQKMI
DDGKLSYEELTSIYLFRIQEHDQNGITLNSVTEINPNAMEEARKLDQERSRNKKSNLYGIPVVVKDNVQTAKVMPTSAGT
YVLKDWIADQDATIVKQLKEEGAFVLGKANMSEWANYLSFTMPSGYSGKKGQNLNPYGPIMFDTSGSSSGSATVVTADFA
PLAVGTETTGSIVAPAAQQSVVGLRPSLGMVSRTGIIPLAETLDTAGPMARTVKDAATLFNAMIGYDEKDVMTEKVKDKE
RIDYTKDLSIDGLKGKKIGLLFSVDQQDENRKAVAEKIRKDLQDAGAILTDYIQLNNGGVDNLQTLEYEFKHNVNDYFSQ
QKNVPVKSLEEIIAFNKKDSKKRIKYGQTLIEASEKSAITKDEFKKVVQTSQENAKKELDRYLVEKGLDALVMINNEEVL
LSAVAGYPELAVPAGYDNNGEPVGAVFVGKQFGEKELFNIGYAYEQQSKNRKPPKL

Sequences:

>Translated_536_residues
MKKWVKVTLSIAGGIVLVACASGYYVYKNYFPKEPERIVYDKERVLQPIHNQLKGINIENVKIKEKEVVNATVDELQKMI
DDGKLSYEELTSIYLFRIQEHDQNGITLNSVTEINPNAMEEARKLDQERSRNKKSNLYGIPVVVKDNVQTAKVMPTSAGT
YVLKDWIADQDATIVKQLKEEGAFVLGKANMSEWANYLSFTMPSGYSGKKGQNLNPYGPIMFDTSGSSSGSATVVTADFA
PLAVGTETTGSIVAPAAQQSVVGLRPSLGMVSRTGIIPLAETLDTAGPMARTVKDAATLFNAMIGYDEKDVMTEKVKDKE
RIDYTKDLSIDGLKGKKIGLLFSVDQQDENRKAVAEKIRKDLQDAGAILTDYIQLNNGGVDNLQTLEYEFKHNVNDYFSQ
QKNVPVKSLEEIIAFNKKDSKKRIKYGQTLIEASEKSAITKDEFKKVVQTSQENAKKELDRYLVEKGLDALVMINNEEVL
LSAVAGYPELAVPAGYDNNGEPVGAVFVGKQFGEKELFNIGYAYEQQSKNRKPPKL
>Mature_536_residues
MKKWVKVTLSIAGGIVLVACASGYYVYKNYFPKEPERIVYDKERVLQPIHNQLKGINIENVKIKEKEVVNATVDELQKMI
DDGKLSYEELTSIYLFRIQEHDQNGITLNSVTEINPNAMEEARKLDQERSRNKKSNLYGIPVVVKDNVQTAKVMPTSAGT
YVLKDWIADQDATIVKQLKEEGAFVLGKANMSEWANYLSFTMPSGYSGKKGQNLNPYGPIMFDTSGSSSGSATVVTADFA
PLAVGTETTGSIVAPAAQQSVVGLRPSLGMVSRTGIIPLAETLDTAGPMARTVKDAATLFNAMIGYDEKDVMTEKVKDKE
RIDYTKDLSIDGLKGKKIGLLFSVDQQDENRKAVAEKIRKDLQDAGAILTDYIQLNNGGVDNLQTLEYEFKHNVNDYFSQ
QKNVPVKSLEEIIAFNKKDSKKRIKYGQTLIEASEKSAITKDEFKKVVQTSQENAKKELDRYLVEKGLDALVMINNEEVL
LSAVAGYPELAVPAGYDNNGEPVGAVFVGKQFGEKELFNIGYAYEQQSKNRKPPKL

Specific function: Furnishes a means for formation of correctly charged Gln-tRNA(Gln) through the transamidation of misacylated Glu- tRNA(Gln) in organisms which lack glutaminyl-tRNA synthetase. The reaction takes place in the presence of glutamine and ATP through an activa

COG id: COG0154

COG function: function code J; Asp-tRNAAsn/Glu-tRNAGln amidotransferase A subunit and related amidases

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Belongs to the amidase family [H]

Homologues:

Organism=Homo sapiens, GI222831590, Length=258, Percent_Identity=32.5581395348837, Blast_Score=100, Evalue=5e-21,
Organism=Homo sapiens, GI195972892, Length=247, Percent_Identity=32.3886639676113, Blast_Score=96, Evalue=8e-20,
Organism=Caenorhabditis elegans, GI17543272, Length=235, Percent_Identity=29.7872340425532, Blast_Score=91, Evalue=1e-18,
Organism=Caenorhabditis elegans, GI17537465, Length=519, Percent_Identity=22.3506743737958, Blast_Score=77, Evalue=2e-14,
Organism=Saccharomyces cerevisiae, GI6323950, Length=224, Percent_Identity=35.2678571428571, Blast_Score=106, Evalue=1e-23,
Organism=Saccharomyces cerevisiae, GI6319685, Length=187, Percent_Identity=33.6898395721925, Blast_Score=82, Evalue=2e-16,
Organism=Drosophila melanogaster, GI24648113, Length=507, Percent_Identity=25.4437869822485, Blast_Score=107, Evalue=1e-23,
Organism=Drosophila melanogaster, GI24644968, Length=518, Percent_Identity=22.7799227799228, Blast_Score=79, Evalue=7e-15,
Organism=Drosophila melanogaster, GI21356731, Length=277, Percent_Identity=23.826714801444, Blast_Score=77, Evalue=4e-14,
Organism=Drosophila melanogaster, GI24648435, Length=248, Percent_Identity=29.0322580645161, Blast_Score=74, Evalue=2e-13,
Organism=Drosophila melanogaster, GI24648437, Length=248, Percent_Identity=29.0322580645161, Blast_Score=74, Evalue=2e-13,
Organism=Drosophila melanogaster, GI45550774, Length=248, Percent_Identity=29.0322580645161, Blast_Score=74, Evalue=2e-13,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000120
- InterPro:   IPR020556
- InterPro:   IPR004412 [H]

Pfam domain/function: PF01425 Amidase [H]

EC number: 6.3.5.7

Molecular weight: Translated: 59452; Mature: 59452

Theoretical pI: Translated: 6.01; Mature: 6.01

Prosite motif: PS00013 PROKAR_LIPOPROTEIN

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.2 %Cys     (Translated Protein)
2.2 %Met     (Translated Protein)
2.4 %Cys+Met (Translated Protein)
0.2 %Cys     (Mature Protein)
2.2 %Met     (Mature Protein)
2.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKKWVKVTLSIAGGIVLVACASGYYVYKNYFPKEPERIVYDKERVLQPIHNQLKGINIEN
CCCEEEEEEECCCCEEEEEECCCEEEEECCCCCCCCHHHCCHHHHHHHHHHHHCCCCCCC
VKIKEKEVVNATVDELQKMIDDGKLSYEELTSIYLFRIQEHDQNGITLNSVTEINPNAME
EEECHHHHHHHHHHHHHHHHHCCCCCHHHHHHEEEEEEEECCCCCEEEECEECCCCHHHH
EARKLDQERSRNKKSNLYGIPVVVKDNVQTAKVMPTSAGTYVLKDWIADQDATIVKQLKE
HHHHHHHHHHCCCCCCEEEEEEEEECCCCEEEEECCCCCHHHHHHHHCCCCHHHHHHHHH
EGAFVLGKANMSEWANYLSFTMPSGYSGKKGQNLNPYGPIMFDTSGSSSGSATVVTADFA
CCCEEEECCCHHHHHHHHEEECCCCCCCCCCCCCCCCCCEEEECCCCCCCCEEEEEECCC
PLAVGTETTGSIVAPAAQQSVVGLRPSLGMVSRTGIIPLAETLDTAGPMARTVKDAATLF
CEEECCCCCCCEECCCHHHHHHCCCCCCCCHHCCCCCCHHHHHHCCCHHHHHHHHHHHHH
NAMIGYDEKDVMTEKVKDKERIDYTKDLSIDGLKGKKIGLLFSVDQQDENRKAVAEKIRK
HHHHCCCHHHHHHHHHHHHHHCCCCCCCCCCCCCCCEEEEEEEECCCCCHHHHHHHHHHH
DLQDAGAILTDYIQLNNGGVDNLQTLEYEFKHNVNDYFSQQKNVPVKSLEEIIAFNKKDS
HHHHHHHHHHHHEEECCCCCCCHHHHHHHHHCCHHHHHHHCCCCCHHHHHHHHHCCCCCC
KKRIKYGQTLIEASEKSAITKDEFKKVVQTSQENAKKELDRYLVEKGLDALVMINNEEVL
HHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEECCCCEE
LSAVAGYPELAVPAGYDNNGEPVGAVFVGKQFGEKELFNIGYAYEQQSKNRKPPKL
EHHHCCCCCEECCCCCCCCCCEEEEEEECCCCCCHHHHHCCCHHHHHHCCCCCCCC
>Mature Secondary Structure
MKKWVKVTLSIAGGIVLVACASGYYVYKNYFPKEPERIVYDKERVLQPIHNQLKGINIEN
CCCEEEEEEECCCCEEEEEECCCEEEEECCCCCCCCHHHCCHHHHHHHHHHHHCCCCCCC
VKIKEKEVVNATVDELQKMIDDGKLSYEELTSIYLFRIQEHDQNGITLNSVTEINPNAME
EEECHHHHHHHHHHHHHHHHHCCCCCHHHHHHEEEEEEEECCCCCEEEECEECCCCHHHH
EARKLDQERSRNKKSNLYGIPVVVKDNVQTAKVMPTSAGTYVLKDWIADQDATIVKQLKE
HHHHHHHHHHCCCCCCEEEEEEEEECCCCEEEEECCCCCHHHHHHHHCCCCHHHHHHHHH
EGAFVLGKANMSEWANYLSFTMPSGYSGKKGQNLNPYGPIMFDTSGSSSGSATVVTADFA
CCCEEEECCCHHHHHHHHEEECCCCCCCCCCCCCCCCCCEEEECCCCCCCCEEEEEECCC
PLAVGTETTGSIVAPAAQQSVVGLRPSLGMVSRTGIIPLAETLDTAGPMARTVKDAATLF
CEEECCCCCCCEECCCHHHHHHCCCCCCCCHHCCCCCCHHHHHHCCCHHHHHHHHHHHHH
NAMIGYDEKDVMTEKVKDKERIDYTKDLSIDGLKGKKIGLLFSVDQQDENRKAVAEKIRK
HHHHCCCHHHHHHHHHHHHHHCCCCCCCCCCCCCCCEEEEEEEECCCCCHHHHHHHHHHH
DLQDAGAILTDYIQLNNGGVDNLQTLEYEFKHNVNDYFSQQKNVPVKSLEEIIAFNKKDS
HHHHHHHHHHHHEEECCCCCCCHHHHHHHHHCCHHHHHHHCCCCCHHHHHHHHHCCCCCC
KKRIKYGQTLIEASEKSAITKDEFKKVVQTSQENAKKELDRYLVEKGLDALVMINNEEVL
HHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEECCCCEE
LSAVAGYPELAVPAGYDNNGEPVGAVFVGKQFGEKELFNIGYAYEQQSKNRKPPKL
EHHHCCCCCEECCCCCCCCCCEEEEEEECCCCCCHHHHHCCCHHHHHHCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: ATP; Glu-tRNA(Gln); L-glutamine

Specific reaction: ATP + Glu-tRNA(Gln) + L-glutamine = ADP + phosphate + Gln-tRNA(Gln) + L-glutamate

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 11466286 [H]