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The map label for this gene is asnO [H]

Identifier: 218902919

GI number: 218902919

Start: 1699562

End: 1700530

Strand: Reverse

Name: asnO [H]

Synonym: BCAH820_1802

Alternate gene names: 218902919

Gene position: 1700530-1699562 (Counterclockwise)

Preceding gene: 218902922

Following gene: 218902918

Centisome position: 32.07

GC content: 38.08

Gene sequence:

>969_bases
ATGTGTGGAATTACAGGTTGGGTAGATTGGAAAGAAGACCTTTCAAATCAACATGTTATTTTAAAAAAGATGGCAAATCG
TATTCAGCATCGCGGTCCTGATGCTGAAGGATTTTGGTTTTCACCTCGTGCGGCCTTTGCACACCGTCGTTTAATTGTAA
TTGATCCAGAAGGCGGGGCACAGCCGAAGACATTTCGTGCTGGTGATTATACGTATGCTCTTACTTATAATGGAGAAATT
TATAATTTCCGTGAGCTGAGAGAACAACTAAGGAAATGTGGTCATACATTTGAAACGCATTCAGATACAGAAGTATTACT
ACATGCTTATTTAGAATGGAAAGAAGACTGCGTGCAACATTTAAACGGCATTTTCGCTTTCGCCTTATGGGATGATCAGA
AGCAACGACTGTTTTTAGCACGTGATCATTTAGGTGTAAAACCGCTCTTTTATACAGAAAGAAATGACAGTATTATTTTC
GGATCTGAAATAAAAGCATTATTAGCTCATCCATCTGTTCCTGCTGAAATTGATGCGAATGGCATAAATGAAATATTTGG
ATTAGGGCTATTTCGAACTCCAGGATGTGGCGTCTTTAAACATATTGAAGAAGTGCGTGCCGGACATTCTATCACATTTA
CACGCGATAAAAAAGTAGTTACAAAGTATTGGAATTTAGAAAGTAAGGTCCATACAGATTCTATAGAAGATACATCATCA
CATATTTTATCTATTTTACAAGATACAGTAAAAAGACAATTAATTGCCGACGTCCCTCTCGTTTGCATGTTATCAGGAGG
GTTAGATTCTAGCGGTATTACTGCACTAGCAGGGAAAGAATTTGCTGCTGAAAATAAAACACTTCATACGTATTCCGTTG
ACTTTGTAAATAGTGCAAAAGATTTCGAGCTGACATTTGCTCGCACTAGTTTAGACGCTCCTTGGGTAAAACGCGTTTCG
AACATGTAG

Upstream 100 bases:

>100_bases
AATTTGATTCATGTAGTACACTATTTATATAGTCATTCTTTAACTTAGTGTACATTTTATTTCGTTAATCTACTATTTTA
AATTAGGAGATGAGATGTGT

Downstream 100 bases:

>100_bases
GGACATCTCATCATGATATTATTGTGAATGCTGAAGAATTAGCAAATCACTTATTCGTTCCCCTCCGTGCAAAAGATTTA
CCGAGCGCTGGCGAAATGGA

Product: asparagine synthetase, glutamine-hydrolyzing

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 322; Mature: 322

Protein sequence:

>322_residues
MCGITGWVDWKEDLSNQHVILKKMANRIQHRGPDAEGFWFSPRAAFAHRRLIVIDPEGGAQPKTFRAGDYTYALTYNGEI
YNFRELREQLRKCGHTFETHSDTEVLLHAYLEWKEDCVQHLNGIFAFALWDDQKQRLFLARDHLGVKPLFYTERNDSIIF
GSEIKALLAHPSVPAEIDANGINEIFGLGLFRTPGCGVFKHIEEVRAGHSITFTRDKKVVTKYWNLESKVHTDSIEDTSS
HILSILQDTVKRQLIADVPLVCMLSGGLDSSGITALAGKEFAAENKTLHTYSVDFVNSAKDFELTFARTSLDAPWVKRVS
NM

Sequences:

>Translated_322_residues
MCGITGWVDWKEDLSNQHVILKKMANRIQHRGPDAEGFWFSPRAAFAHRRLIVIDPEGGAQPKTFRAGDYTYALTYNGEI
YNFRELREQLRKCGHTFETHSDTEVLLHAYLEWKEDCVQHLNGIFAFALWDDQKQRLFLARDHLGVKPLFYTERNDSIIF
GSEIKALLAHPSVPAEIDANGINEIFGLGLFRTPGCGVFKHIEEVRAGHSITFTRDKKVVTKYWNLESKVHTDSIEDTSS
HILSILQDTVKRQLIADVPLVCMLSGGLDSSGITALAGKEFAAENKTLHTYSVDFVNSAKDFELTFARTSLDAPWVKRVS
NM
>Mature_322_residues
MCGITGWVDWKEDLSNQHVILKKMANRIQHRGPDAEGFWFSPRAAFAHRRLIVIDPEGGAQPKTFRAGDYTYALTYNGEI
YNFRELREQLRKCGHTFETHSDTEVLLHAYLEWKEDCVQHLNGIFAFALWDDQKQRLFLARDHLGVKPLFYTERNDSIIF
GSEIKALLAHPSVPAEIDANGINEIFGLGLFRTPGCGVFKHIEEVRAGHSITFTRDKKVVTKYWNLESKVHTDSIEDTSS
HILSILQDTVKRQLIADVPLVCMLSGGLDSSGITALAGKEFAAENKTLHTYSVDFVNSAKDFELTFARTSLDAPWVKRVS
NM

Specific function: Asparagine synthetase involved in sporulation [H]

COG id: COG0367

COG function: function code E; Asparagine synthase (glutamine-hydrolyzing)

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 glutamine amidotransferase type-2 domain [H]

Homologues:

Organism=Homo sapiens, GI168229248, Length=311, Percent_Identity=32.475884244373, Blast_Score=120, Evalue=2e-27,
Organism=Homo sapiens, GI168229252, Length=311, Percent_Identity=32.475884244373, Blast_Score=120, Evalue=2e-27,
Organism=Homo sapiens, GI168229250, Length=311, Percent_Identity=32.475884244373, Blast_Score=120, Evalue=2e-27,
Organism=Homo sapiens, GI296010848, Length=285, Percent_Identity=32.6315789473684, Blast_Score=115, Evalue=4e-26,
Organism=Homo sapiens, GI296010852, Length=211, Percent_Identity=30.8056872037915, Blast_Score=79, Evalue=6e-15,
Organism=Homo sapiens, GI296010850, Length=211, Percent_Identity=30.8056872037915, Blast_Score=79, Evalue=6e-15,
Organism=Escherichia coli, GI1786889, Length=287, Percent_Identity=33.7979094076655, Blast_Score=133, Evalue=1e-32,
Organism=Caenorhabditis elegans, GI25147560, Length=287, Percent_Identity=27.8745644599303, Blast_Score=103, Evalue=8e-23,
Organism=Caenorhabditis elegans, GI25147557, Length=287, Percent_Identity=27.8745644599303, Blast_Score=103, Evalue=9e-23,
Organism=Caenorhabditis elegans, GI71993933, Length=287, Percent_Identity=27.8745644599303, Blast_Score=103, Evalue=1e-22,
Organism=Saccharomyces cerevisiae, GI6321563, Length=292, Percent_Identity=32.1917808219178, Blast_Score=127, Evalue=2e-30,
Organism=Saccharomyces cerevisiae, GI6325403, Length=301, Percent_Identity=31.2292358803987, Blast_Score=122, Evalue=6e-29,
Organism=Drosophila melanogaster, GI45553209, Length=286, Percent_Identity=30.4195804195804, Blast_Score=110, Evalue=2e-24,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR006426
- InterPro:   IPR001962
- InterPro:   IPR017932
- InterPro:   IPR014729 [H]

Pfam domain/function: PF00733 Asn_synthase [H]

EC number: =6.3.5.4 [H]

Molecular weight: Translated: 36503; Mature: 36503

Theoretical pI: Translated: 6.75; Mature: 6.75

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.6 %Cys     (Translated Protein)
1.2 %Met     (Translated Protein)
2.8 %Cys+Met (Translated Protein)
1.6 %Cys     (Mature Protein)
1.2 %Met     (Mature Protein)
2.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MCGITGWVDWKEDLSNQHVILKKMANRIQHRGPDAEGFWFSPRAAFAHRRLIVIDPEGGA
CCCCCCCCCHHHHCCCCEEHHHHHHHHHHHCCCCCCCCEECCHHHHHCCEEEEECCCCCC
QPKTFRAGDYTYALTYNGEIYNFRELREQLRKCGHTFETHSDTEVLLHAYLEWKEDCVQH
CCCEEECCCEEEEEEECCCEECHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHH
LNGIFAFALWDDQKQRLFLARDHLGVKPLFYTERNDSIIFGSEIKALLAHPSVPAEIDAN
HCCEEEEEEECCCCCEEEEECCCCCCCCEEEECCCCCEEEHHHHHHHHCCCCCCCEECCC
GINEIFGLGLFRTPGCGVFKHIEEVRAGHSITFTRDKKVVTKYWNLESKVHTDSIEDTSS
CHHHHHHCCCCCCCCCHHHHHHHHHHCCCEEEEECCCHHHHHHHCCCCCCCCCCCHHHHH
HILSILQDTVKRQLIADVPLVCMLSGGLDSSGITALAGKEFAAENKTLHTYSVDFVNSAK
HHHHHHHHHHHHHHHHCCCEEEEEECCCCCCCEEEECCCHHHCCCCEEEEEEEEECCCCC
DFELTFARTSLDAPWVKRVSNM
CEEEEEEECCCCCHHHHHHCCC
>Mature Secondary Structure
MCGITGWVDWKEDLSNQHVILKKMANRIQHRGPDAEGFWFSPRAAFAHRRLIVIDPEGGA
CCCCCCCCCHHHHCCCCEEHHHHHHHHHHHCCCCCCCCEECCHHHHHCCEEEEECCCCCC
QPKTFRAGDYTYALTYNGEIYNFRELREQLRKCGHTFETHSDTEVLLHAYLEWKEDCVQH
CCCEEECCCEEEEEEECCCEECHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHH
LNGIFAFALWDDQKQRLFLARDHLGVKPLFYTERNDSIIFGSEIKALLAHPSVPAEIDAN
HCCEEEEEEECCCCCEEEEECCCCCCCCEEEECCCCCEEEHHHHHHHHCCCCCCCEECCC
GINEIFGLGLFRTPGCGVFKHIEEVRAGHSITFTRDKKVVTKYWNLESKVHTDSIEDTSS
CHHHHHHCCCCCCCCCHHHHHHHHHHCCCEEEEECCCHHHHHHHCCCCCCCCCCCHHHHH
HILSILQDTVKRQLIADVPLVCMLSGGLDSSGITALAGKEFAAENKTLHTYSVDFVNSAK
HHHHHHHHHHHHHHHHCCCEEEEEECCCCCCCEEEECCCHHHCCCCEEEEEEEEECCCCC
DFELTFARTSLDAPWVKRVSNM
CEEEEEEECCCCCHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9353931; 9384377; 10498721 [H]